Mycoscience
Online ISSN : 1618-2545
Print ISSN : 1340-3540
49 巻, 6 号
選択された号の論文の11件中1~11を表示しています
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  • Akiko Yamagashira, Chihiro Iwai, Masahiko Moroishi, Masakazu Misaka, Y ...
    2008 年 49 巻 6 号 p. 351-358
    発行日: 2008年
    公開日: 2023/03/31
    ジャーナル オープンアクセス

    The population structure of Magnaporthe oryzae from green foxtail (Setaria viridis) in Japan was examined by DNA fingerprint analyses using the transposable elements MGR586 and MAGGY as probes. Fifteen M. oryzae isolates from green foxtail were collected from 11 Japanese prefectures so that a macrogeographic population of this pathogen is represented. All the 15 isolates were sorted into distinct haplotypes by DNA fingerprint analyses with both probes. Furthermore, similarities between the DNA fingerprint profiles of the 15 isolates were exclusively low; i.e., if lineages are arbitrarily established based on greater than 70% similarities in isolates, the 15 isolates could be categorized into 13 distinct lineages by DNA fingerprinting with both probes. We also examined the MGR586 DNA fingerprint variations of this pathogen in 9 microgeographic populations each of which contained 20 to 24 isolates collected from a 1 m2 or 50 m2 area. In all the 9 populations, more than 2 haplotypes, which shared less than 70% similarities, were identified in the DNA fingerprint profiles. These results suggested that M. oryzae isolates from the green foxtail in Japan possessed a complex lineage structure, even at the microgeographic scale.

  • Izumi Okane, Prasert Srikitikulchai, Kyoko Toyama, Thomas Læssøe, Soms ...
    2008 年 49 巻 6 号 p. 359-372
    発行日: 2008年
    公開日: 2023/03/31
    ジャーナル オープンアクセス

    A study of the diversity, taxonomy, and ecology of endophytic Xylariaceae (Ascomycota) was carried out. In this study, we obtained isolates of Xylariaceae from healthy, attached leaves and teleomorphic stromata on decayed plant materials in a permanent plot at Khao Yai National Park (Thailand). In addition, strains deposited beforehand were selected in which both endophytic strains isolated from living plant tissues and saprobic strains from fruit bodies were included. Consequently, 405 strains of Xylariaceae (273 endophytic and 132 saprobic strains, including identified strains) were studied to reveal the diversity and taxonomy of endophytes and the relationships between those endophytes and saprobic Xylariaceae in Thailand that have been recorded according to fruit-body formation on decayed plant materials. Analysis of 28S rDNA D1/D2 sequences revealed 21 xylariaceous species inhabiting tropical foliage at the site, and several species that are already known as saprobes appear to be among those isolated from living leaves. Furthermore, several clades that consisted of only endophytic strains were found, and some of these have no known matches in public DNA sequence banks.

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