Using Starr's type ballistic bed modified by H. A. Blaire, cause of the origin of K wave and effects of various depressant agents were studied in arterial hypertension. Keith-Wagener's classification was also investigated hemodynamically. Thus the following results were gained. 1) In hypertension, deepening of K wave and increase in respiratory variation were more frequently observed. K wave was assumed to be the resultant of peripheral arteriolar resistance, of impact wave in the aorta, of bounding wave of the aortic arch and of impact wave of the atrioventricular septum. 2) An acute administration of Pendiomide, Vegolysen-T and Ansolysen reduced stroke volume and minute volume, but JK/IJ was increased in many cases. Apresoline and Methobromine showed frequently the opposite results, however, decrease in the systemic blood pressure did not always correspond to the change of JK/IJ, which would be due to different occurrence of the homeostasis in the medication of different kind of agents. 3) Keith-Wagener's classification established from point of view of the morbid course does not clarify the expression of the decompensated states in hypertensive cases. As to this question there were performed the statistical and hemodynamical investigations.
It is a well-known fact that the variation of serum protein arises at athletic exercises and sports proteinuria appears for a moment after severe exercises. At this research, the variation of serum protein was studied with the electrophoresis method in various types of athletic exercises. The electrophoresis method was carried out with sports proteinuria concentrated by lyophilization. 1) In serum protein the increase of albumin and total protein was found immediately after exercise. In globulin, it was indicated either a decreased or an invariable value immediately after exercise. The greater the increase of total protein and albumin, the more marked decrease of globulin was observed. γ-globulin decreased more markedly than α-globulin and β-globulin. However, in 45 minutes after exercise, it recovered to value before exercise. The variation is greatest in 10, 000 metre race and decreases in the following order: 3, 000 metre race, modified Master's two-step test, medium boating-exercise, bicycle-ergometer and slight boating-exercise. 2) As to the effects of application of Cortisone, ACTH and ACE for some examine with modified Master's two-step test, no striking variation was found with ACE in comparison to the control cases without ACE. In ACTH and Cortisone cases, total protein and albumin immediately after exercise indicated a slight increase in comparison to the control cases. At the same time, it was found the increase of globulin in ACTH and Cortisone cases too. However, in 45 minutes after exercise, it recovered to value before exercise. 3) In most cases the fractions of sports proteinuria was examined as in the case of renal disease consist of de-fibrinogenated plasma albumin, α-globulin, β-globulin and γ-globulin. According to the degree of various exercises, it was found the variation in composition of the fractions.
In-vitro experiment was conducted on the action of tropolone (Trp) and its eight derivatives against Japanese B encephalitis virus (Nakayama strain). The substance to be examined, dissolved in tween 80, was added to the supernatant fluid obtained by ultracentrifuging the brain emulsion of virus-inoculated mice. After incubating at 22°C for 1 hour, the mixture was intracerebrally inoculated into a fixed number of mice. Comparing the result with the control, logarithm difference (log diff.) of LD50 was computed. Antiviral potency is represented by -log diff. The result was as follows. At 1000 γ/cc the following values were registered: Hinokitiol (Hi) (-log diff.=4.2), Hi-natrium (2.5), Trp (4.1), 3-monobromotrp (3.2), 3-5-dibromotrp (3.3), 3-7-dibromotrp (5.3), 3-5-7-tribromotrp (4.9), mercaptotropone (2.0) 3-5-7-tribromo-2-aminotropone showed no potency against virus dilutions stronger than 10-6. Hi, Trp, 3-7-dibromotrp and 3-5-7-tribromotrp showed considerable potency at 100 γ/cc. Even at 10γ/cc Hi and Trp registered a slight potency. On the other hand, it was noted that the antiviral potency of 3-monobromotrp and 3-5-dibromotrp at 100 γ/cc was very weak, and that Hi-Natrium and mercaptotropone revealed no potency at 100 γ/cc.
The anticoagulant effect of charoninsulfuric acid, a polysaccharide sulfuric acid obtained from the mucus of [Charonia lampas] was investigated both in vivo and in vitro. The results obtained were as follows: 1) Charoninsulfuric acid is a strong anticoagulant. The clotting time of whole blood was prolonged slightly by adding only 1γ charoninsulfuric acid to 1ml. of rabbit blood or 0.1γ to 1ml. of human blood. The more its concentration increased, the longer the clotting time became, and in concentration of over 50γ in 1ml. of rabbit or human blood the clot was not detected over 24 hours. when charoninsulfuric acid was intravenously injected (1 mg per Kg of body weight), the clotting time of rabbit whole blood was prolonged and reached the maximal level after 15 minutes. Then, the clotting time decreased gradually to normal level within 1 hour or so. 2) In experiments in vitro, charoninsulfuric acid produced no remarkable prolongation of prothrombin time of rabbit plasma by adding charoninsulfuric acid up to 20γ per ml. of blood, while prothrombin time of human plasma was prolonged by adding only 0.05γ per ml. of blood and the more the concentration increased, the more the prothrombin time was prolonged. Prothrombin time of rabbit plasma measured before and after the intravenous injection of charoninsulfuric acid (1mg. per Kg. of body weight) revealed no remarkable change. 3) Stable factor (SPCA) was not affected by charoninsulfuric acid both in vitro and in vivo. 4) Charoninsulfuric acid has not only anti-labile factor activity but also anti-prothrombin activity. 5) Antithromboplastic activity of charoninsulfuric acid was demonstrated by thromboplastin generation test both of Matsuoka-Yamanaka and of Biggs-Douglas. 6) Thromboplastin dilution curve of rabbit plasma, which means the prothrombin time measured with serial twofold diluted rabbit brain extract, was also prolonged after the intravenous injection of charoninsulfuric acid (1mg. per Kg. of body weight). 7) When charoninsulfuric acid was intravenously injected (1mg. per Kg. of body weight), leucocyte and platelet count of rabbit were temporarily decreased, while the erythrocyte count showed no remarkable change.
In 25 cases of unexpandable lung treated with artificial pneumoperitoneum to close the pleural dead space, clinical studies on mediastinal shift and mediastinal hernia were performed and following results were obtained: 1) Either mediastinal shift or mediastinal hernia was observed in 21 cases (81.0%), while only 4 cases (16.0%) were without both of them. 2) The longer the pleural dead space existed, the more often mediastinal shift and hernia was found. 3) In most of cases with remarkable mediastinal shift, mediastinal hernia was not or only in slight degree observed, while remarkable hernia was likely to be observed in cases without or with mediastinal shift of slight degree. 4) Mediastinal shift, which was additionally increased or newly formed by artificial pneumoperitoneum, was observed in 14 of 25 cases (56.0%). In 5 cases with mediastinal hernia the degree of hernia was increased in only one case by artificial pneumoperitoneum. 5) The dead space was closed more earlier in cases without mediastinal shift or hernia than in cases with them. In some patients among the latter the dead space failed to be closed. 6) Function of the affected lung was not improved even after the dead space had been closed. And the dysfunction of the opposite lung, which happened to occur due to expected overexpansion, was not observed in most cases. 7) The lung wss forced to be deformed and expanded in horizontal axis as the result of the collaps in apico-caudal axis due to artificial pneumoperitoneum. In this case the deformity of the lung was observed not only in the affected side with dead space, but also in the opposite side. Mediastinal shift or hernia is thought to be formed under these circumstances. These factors seems to play a great role of closing pleural dead space.