In the previous study, abnormal fluorescent substances which appear in the urine of patients of gastrointestinal diseases and their relationship with FAD were investigated by means of paper partition chromatography, and indoxyl sulfate was determined by absorptive phenomenon on Rf. 0.55, kynurenine showed bluish fluorescent spot on Rf. 0.45 under ultraviolet irradiation; and these two substances were observed to decrease after the administration of FAD. Therefore, relationship between FAD and metabolism of indole nucleus was experimentally investigated. Following results were obtained: 1) In the urine of normal rabbits, the excretion of metabolite of tryptophan system showed considerable fluctuation individually and daily. Dietary influences were, however, more marked: it was concluded that vitamins in the food, especially riboflavin, pyridoxine, folic acid and ascorbic acid showed much influences. 2) When tryptophan was given to rabbits, the urinary riboflavin showed increases two days after the cessation of the tryptophan administration. 3) Urinary excretion of indican in the patient with diseases of gastrointestinal system was confirmed to increase more than in normal person, and to decrease by the addition of FAD. 4) When indole was given orally to normal rabbits, excretion of urinary indican and urinary anthranilic acid increased. In case where FAD was given in addition, oxidation of indole to indican was acceralated and this indican was excreted more rapidly. The amount of indican was decreased by the addition of FAD. 5) When indole was given to the rat subcutaneously, indican content in the blood, liver, kidneys and lungs increased. If FAD was added to indole, indican content in them elevated in the early stage and then decreased more rapidly. In the other hand, tryptophan, kynurenine and anthranilic acid in the liver showed some tendency of increase after the administration of indole.
E. E. G. in 101 cases suffering from essential hypertension were observed. The results can be classified into four groups according to the E. E. graphic patterns. Group A; Cases without any pathologic patterns in E. E. G.. Group B ; Cases with slight pathologic patterns in E. E. G.. Group C; Cases characterized with rapid waves; the mean period 84 msec. Group D; Cases with the appearance of slow waves. Following results are obtained, that is to say, group A is 36%, group B. 13%, group C. 36%, group D. 15% of all cases examined. 1) In essential hypertension there can not be observed any special E. E. graphic pattern. 2) There exists no clear correlation between E. E. graphic patterns and chief claims, renal function, Ecg., the classification according to K. W., findings of fuudus oculi, B. P. of central retinal artery, arterial B. P.. 3) Relationship between circulatory dynamics and E. E. G.; Group A are frequently found in W+E types and M types are often determined in group D. 4) In some cases, the E. E. graphic patterns show the changes of brain more clearly than the other clinical findings do. 5) The appearance of abnormal waves can be examined more closely by means of deep respiration and breathholding test than of merely deep respiration. 6) No special change of E. E. G. is observed in the breathholding test in health. 7) The breathholding test examined in essential hypertension reveals the occurrence of rapid waves “en salve” with large amplitude. 8) In the breathholding, test observed in epilepsy, there occurs spike and wave during hyperpnea, while it disappears during apnea. 9) The breathholding test after medication of priscol. It is apt to turn the slow waves found in essential hypertension into normal pattern or into rapid waves, the same procedure performed in epilepsy shows no changes in E. E. G.. 10) Breathholding test after the use of Kallikrein facilitates the appearance of α waves and inhibits the rapid waves. 11) Low % and 100% of O2 were inhaled by the same individual; E. E. G. found in both examinations are similar patterns. Improvement or deterioration of E. E. graphic patterns can not pararell to those of clinical findings. This suggests that E. E. graphic changes must be not only understood medicaly, but also psychologically. 12) Apresoline turnes often the pathologic E. E. G. observed in es sential hypertension into normal pattern; one of important causes is thought to be the increase in the cerebral blood flow. Slow waves can be not frequently recognized immediately before the attack of hypertensive encephalopathy. 13) The degrees of lowering of blood pressure in the patients who are medicated with R. S. is remarkable roughly in proportion to the increase in mean period and in mean amplitude. In addition it may be pointed out that R. S. does not reveal sleep spindle. 14) R. S.-E. E. graphic pattern is quite similar to the pattern in the medication of Deserpidine.
1. There were many feverish cases in the patients of malignant tumor in which no complications had been found; and these fever-types were febris remittens et intermittens, febris continua, fever paroxysm similar to malaria, inconstant fever and slight fever etc., slight fever cases were more often seen. 2. After administrating antitumor-drugs to patients with malignant tumor, there were cases in which fever-types changed. Some cases with probably some pyrogenic substances in their malignant tumor tissue were observed. 3. After an injection of the water extract of malignant tumor tissue into the auricular vein of rabbits, increase of the body temperature was observed; on the other hand no variation of body temperature was observed by injecting intravenously the water extract of normal muscle tissue of a tumor-bearing-animal. 4. Increase of body temperature was observed immediately after injection of some dosis of the polysaccharide fraction extracted from the malignant tumor tissue into the auricular vein of rabbits; hypothermia appeared at first with remarkable increase of body temperature thereafter when the polysaccharide fraction was injected into the regio hypothalamica of rabbits. 5. No pyrogenic action was observed in the protein fraction or phospholipid fraction extracted from the malignant tumor tissue but was observed in the polysaccharide fraction extracted from the normal muscle tissue of tumor-bearing-animals. 6. Nitrogen-mustard N-oxide (MBAO) depressed the pyrogenic action of the polysaccharide fraction extracted from malignant tumor, but glucuronic acid, saccharogen and actinomycin did not influence the pyrogenic action of the abovementioned substance. 7. Among the polysaccharide fractions extracted from the malignant tumor administered with MBAO, glucuronic acid and actinomycin, there were cases in which the-pyrogenic action of these polysaccharide fractions decreased; the decrease of the pyrogenic action of the same substances extracted from the tumor administered both cysteine and large dosis of MBAO was more remarkable, probably due to the protective action of cysteine for MBAO.
I performed the study on kidney function for the purpose of clearing the genesis of edema. In the patients with edematous diseases, I measured GFR, RPF, TmPAH, TmG, electrolytes (Na, K, Cl) clearance and antidiuretic substance (ADS) in serum and urine. First, I compared the kidney function of edematous states with that of non-edematous states. Generally, GFR was less than about 80 cc/min. in the edematous cases, but edema did not always appear below this value. RPF, TmG, TmPAH also decreased in edematous states. From these facts, the genesis of edema is not cleared by only the decrease in the kidney function. Then, I supposed that humoral factors take some parts. I noticed that ADS increased in serum and urine of edematous states and that this increase was not paralleled to the grade of edema in some cases. In edematous states, Na, Cl clearances decreased and K clearance did not show a constant change. Next, I studied the diuretic effects in edematous diseases when many diuretics and organ preparates were administerd to edematous patients. Digitalis glycosides, mercurial diuretics, diamox, mictine and theophylline preparates, and cortisone, insulin and pitressin were used for this study. Each preparate had various effect upon the kidney function. Then, I observed the action of heat and rectangular low frequency current upon the human kidney. In 19 cases, antityphoid vaccine and TTG were inoculated and in 21 cases hot bathing, cold pressor test, spinal electrification of low frequency of rectangular pulse were examined. In many cases, the kidney function temporarily decreased and ADS of serum increased. This condition occured shortly after the administration of drug and electrification and appeared a kind of shock. I used TTG for the fever therapy, and in the rectangular low frequency current therapy I regulated very slowly the grade of the electrification at the beginning and the end. Then I noticed that the kidney function was scarecely varied.