Mixed addition of both erythrosine (ES) and N-phenylthioacridone (TA) is effective to sensitize azides, though ES has no effect in sensitization of azides. This super additivity in sensitization suggests a complex formation between ES and TA. Then, the interaction between both compounds was investigated by spectroscopic measurements in absorption and emission. Addition of TA to ES in ethanol gives a new absorption band near at 600nm. The new band is caused by a complex formation between ES and TA. The complex absorption can not be observed in a case between ES and N-phenylacridone (PA) which has almost the same chemical structure as TA except of a difference between oxygen and sulfur atom. This fact indicates an important role of sulfur atom in the structure for the complex formation between ES and TA. The Benesi-Hildebrand equation for the complex absorption gives the molecular extinction coefficient of the complex to be 9430 (mol/
l)
-1cm
-1 and the association constant to be 279 (mol/
l)
-1. In order to confirm the complex formation, the fluorescence quenching of ES by addition of TA was also investigated. The Stern Volmer plots showed a linear relation in the low concentration region of TA, and stronger quenching in the high concentration than that expected from the relation. The slope for the Stern Volmer plots gave the association constant between ES and TA to be 290 (mol/
l)
-1. The resulted value was well coincident with the value estimated from the absorption spectruum of the associated complex. The temperature dependence of fluorescence quenching for the associated complex indicated -38.24kJ mol
-1 for
ΔHc and -81.21J mol
-1K
-1 for
ΔSc. Thus, the fluorescence quenching and the complex formation support existence of a strong interaction between ES and TA. It is concluded that the complex formation is a main reason for the super additivity in sensitization of azides by ES and TA.
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