Premalignant fibroepithelial tumor occurred in a 67-year-old woman. The lesion clinically and histologically characteristic of the disease was found on the right upper part of the pubic area. Other two lesions diagnosed as mixed carcinoma and basal squamous cell carcinoma coexisted on the abdomen.
This report describes the analytical data of skin surface lipid and comedo of Yusho (chlorobiphenyl poisoning) patients by thin layer chromatography and gas chromatography. 1) In a female group of Yusho patients, triglyceride content was decreased. It was assumed to be the results of decreasing of triglyceride production from sebaseous gland. 2) Lipid metabolism of keratin in comedo of Yusho was seemed to have a great quantity of keratin. 3) The content of palmitic acid and palmitoleic acid of skin surface lipid and comedo was decreased, comparing with controls in Yusho. 4) Differences of lipid metabolism between Yusho and Acne vulgaris were observed: the hypofunction of normal sebaseous gland distributed at whole skin surface of Yusho was the significantly important symtom in contrast to that of the sebaceous gland of Acne vulgaris only with comedo.
Enzyme activities in the human fetal skin of phosphorylase (P-lase), NADP-linked glucose-6-phosphate dehydrogenase (NADP-G-6PDH), NAD-linked lactic dehydrogenase (NAD-LDH), alkalin phosphatase (AlP-tase), acid phosphatase (AcP-tase), NAD-linked isocitric dehydrogenase (NAD-ICDH), succinic dehydrogenase (SDH), cytochrome oxidase (Cyt O), NAD-linked malic dehydrogenase (NAD-MDH), beta-glucuronidase (B-G), non-specific esterase (NsE) and leucine aminopeptidase (LAP) were histochemically detected during the development from fourth to seventh month. All of these enzyme reactions were gradually increased as the human embryos grew older. Concerning to these enzyme reactions two different patterns of activities were observed. One of these is a group of functional enzymes, which are related to the fundamental physiologic function of embryonic tissues or cells. These enzyme activities are increased as the embryos grow older and still demonstrated even in the adult human skin. Another is a group of embryonic enzymes, which are demonstrated at a certain time during the embryonic development but not in adult human skin. Functional ones are twelve enzymes above mentioned and embryonic enzymes are AlP-tase in epidermal basal layer, AcP-tase in hair papillae and con-nective tissue cells and NAD-ICDH, NAD-MDH and LAP in periderms. In comparison with the intensity of reaction between embryonic and adult skin, the embryonic epidermis showed stronger activities of AcP-tase and NsE, on the other hand, the epidermis of adult skin showed stronger activities of NAD-LDH, NAD-ICDH, SDH, Cyt O, NAD-MDH, B-G and LAP. The reaction of P-lase and NADP-G-6PDH revealed no differences between embryonic and adult skin. Glycolytic pathways during embryonic development are predominantly Embden-Meyerhof pathway and hexose monophosphate shunt, on the other hand, citric acid cycle in adult skin. Enzyme histotopography showed that enzyme activities of Embden-Meyerhof pathway are found in whole epidermis (whole layer active type), those of citric acid cycle were noticed in lower layer of epidermis (lower layer active type) and those of hexose monophosphate shunt or lysosome were observed in upper layer of epidermis (upper layer active type) and in sebaceous glands. These findings may indicate that reduced NADP which is produced by hexose monophosphate shunt is related to the synthesis of fatty acids and steroids in sebaceous glands and is used for the reaction of glutathion reductase in epidermis. Lysosomal enzymes may be related to the autolysis of cells of sebaceous glands.
Histochemical studies were reported on D-glucuronolactone dehydrogenase, the enzyme of the metabolic pathway from D-glucuronic acid to D-glucaric acid, dealing with the normal and diseased skin. The following results were obtained. 1) It was found that the optimum incubation medium for the assay was as follows; glucuronolactone 100mg, DPN 8mg, 1/15M phosphate buffer (pH 7.8) 9ml and distilled water 1ml. 2) The existence of activities of D-glucuronolactone dehydrogenase was found to be distributed in cytoplasm of cells of the granular layer, the Malpighii layer, the inner and the outer hair sheaths, the sebaceous gland, the eccrine sweat gland and duct and fibroblasts of the normal human skin. The similar findings were also observed in mouse skin. 3) Comparative observations between activities of D-glucuronolactone dehydrogenase and beta-glucuronidase in the skin with pathological changes, were done. It was resulted that localization of both enzymes was found to be almost identical, while activities was shown to be parallel or adverse each other. These findings may presume that D-glucuronolactone dehydrogenase plays a role as a feed back control enzyme of beta-glucuronidase.