The synthesis of plasma albumin was studied in perfused livers of normal and chronic CCl4 intoxicated rats with carbonate-14C. Simultaneously, he perfused livers were studied histologically. Livers of normal and the intoxicated rats were perfused with heparinized rat blood diluted with Krebs-Henseleit bicarbonate buffer for 3 hours. The rate of albumin synthesis was calculated from the specific activity of newly formed urea and the total 14C in guanido carbon of arginine in albumin. The intoxicated livers were classified morphologically by their stages into 5 groups, i.e., group A (acute stage), B (nonseptal fibrotic stage), C (septal fibrotic stage), D (cirrhotic stage) and E (cirrhotic stage with ascites). Serum albumin concentration decreased significantly in proportion to the progress of the intoxication, and it was observed remarkably in group E. Comparing with normal liver, the rate of albumin synthesis decreased in group A, but improved in group B. According to the stages of intoxication from group C to E, the synthesis rate decreased gradually. Besides, no significant changes of serum albumin concentration between group C and D was observed, but the synthesis rate of group D decreased compared with that of C. From these results, the close relation was observed between the albumin synthesis rate and the histologic findings of CCl4 intoxicated liver, especially, necrosis and regeneration of hepatocytes. Moreover, it was observed that there was significant relation between the synthesis rate and serum albumin concentration in the intoxicated rats, and that the decrease of the synthesis was more remarkable than that of albumin concentration from the regression line obtained.
In order to re-evaluate the relationship between gastric acid secretion and the peptic ulcer, gastric analysis had been performed on 48 in-patients consisting of 8 cases of normal, 9 cases of duodenal ulcer and 31 cases of gastric ulcer, under stimulation of gastrin-like tetrapeptide (Tetragastrin). At that time, phenol red dilution method was applied to expect the accuracy of the results. The results were as follows: 1. The ratio of the aspirated volume to the intragastric volume of gastric juice ranged from 24.4 to 94.1%, and had 59.5±16.8% as average. 2. In normal subjects, BSVR, BAO, SVR and MAO were 46.0±16.9ml/30min., 2.79±1.49mEq/30min., 186.7±14.7ml/h. and 19.36±4.27mEq/h. respectively. 3. The capacity of the acid secretion was reduced in proportion to the atrophic gastritis. This was also recognized from the difference of the acid secretion in the patient's age or the location of ulcer. 4. In duodenal ulcer, both basal secretion and the response to Tetragastrin were much higher than those in the normal or gastric ulcer. Furthermore, the ratio of BAO to MAO was large, showing the relative increase in the basal secretion. 5. There was no relationship between the acid secretion and the development or the healing tendency of gastric ulcer. 6. With the healing of gastric ulcer, BAO was gradually decreased, but no change was showed in MAO. In some of the intractable cases of gastric ulcer, BAO was increased progressively.
The effects of several organic anions on Bilirubin excretion were studied in rats with biliary fistula, and the following results were obtained. 1) Pretreatment of rats with phenobarbital increases excretion of endogenous as well as exogenous bilirubin. A study with 3H-delta-aminolevulinic acid has suggested an increase of biliary 3H-bilirubin in an early pnase of the administration of the former, and hence increase of early labeled bilirubin. Increased biliary excretion of unconjugated bilirubin load was seen only shortly after the administration. 2) Novobiocin inhibited the biliary excretion of bilirubin, suggesting an inhibition in the process of bilirubin excretion. The effect was rather short, but competition as substrate for excretion seems rather unlikely. 3) A mutual inhibition for excretion is obvious between BSP and bilirubin. The biliary excretion of BSP was markedly reduced by coadministration of bilirubin, and the reduction was most pronounced in the fraction of nonconjugate BSP during the early phase of administration. The same was true with 131I-BSP. These results indicate that the biliary excretion of bilirubin as a function of hepatocyte is influenced by a number of organic anions and various mechanisms during the transport in the cell, which involves several biochemical processes.