Normal white male rats, weighing about 200gm., were fed on the diet composed of 0.06% 3' Methyl 4 dimethylaminoazobenzene with basal diet. Rats were killed at 4, 8, 12 and 16 weeks after beginning of feeding of this diet, and the livers were removed for succinic dehydrogenase (SDH), Diphosphopiridine Nucleotide diaphorase (DPN), Adenosine Triphosphatase (ATP) and Hematoxyline and Eosine stain. The liver obtained from the rat after 4 weeks feeding showed marked proliferation of oval shaped cells at the portal space of the liver and which showed little activities of SDH and DPN, although the parenchymatous cells showed regeneration but normal zonal difference and normal activities of these enzymes. After 8 or 12 weeks of feeding these oval shaped cells assumed the shape of grandular architecture surrounded by proliferating connective tissue which showed significant ATP activity, and some of the cells which formed the grandular architecture showed irregular in size with significant SDH and DPN activity in the cytoplasma. After 16 weeks of feeding, Those cells which aquired significant SDH and DPN activities became hepatocellular carcinoma and the cells which did not aquire any those activities became cholangiocellular carcinoma. The SDH and DPN activities of the hepatocellular carcinoma were decreased with the grow, and the activities were then located at the cell memblane of the hepatocellular carcinoma.
Roentgenologic, endoscopic, biopsy and cytopathological procedures are generally used as diagnostic measures for detection of gastric cancer. On the other hand, physiological or biochemical measures of diagnosis, such as measurement of 32P uptake are not generalized. This is, especially in the case of gastric carcinoma, because the uptake ratio of 32P of normal mucosa and cancer tissue are not distinctly different and because the fixation of the etecting probe over the gastric lesion is technically difficult. In my method the small GM counter probe, which is accompanied with a gastrofiberscope, is inserted into the stomach under local anesthesia. The tip of the probe is controlled and fixed over the mucosal lesion under the direct vision controll of the gastrofiberscope, and the 32P uptake of various area are counted. This method is divised for the purpose of detecting the malignancy according to the uptake difference of 32P of normal and carcinoma tissues. Ten cases of benign gastric lesions and 17 cases of gastric carcinoma are measured. Right diagnostic ratios are 100% on benign lesions and 76.5% on gastric carcinoma. The relationship between the 32P uptake ratio and microscopic features are studied. Schirrhous and mutinous carcinoma with small cancer cell density are usually small in uptake ratio of 32P in my study.