The content of glucaric acid was evaluated in 72 gallbladder bile specimens from patients with gastric diseases, gallstones and other biliary tract diseases. Also total bilirubin, activity of β-glucuronidase and bacterial infection were studied in all specimens.
(1) The content of glucaric acid estimated was 200.7, 78.1 and 40.0μg/ml on an average, in control group, cholesterol stone cases and calcium bilirubinate stone cases, respectively. Apparently these gallstone patients had a lower concentration of glucaric acid than control group. In comparison with this, the specimens taken from pure pigment stone cases and calculous hemolytic jaundice cases contained much the same or rather more amount of glucaric acid.
(2) Specimens from control group, cholesterol stone cases and calcium bilirubinate stone cases contained total bilirubin in the concentration range of 100-700, 0-250 and 14-80mg/dl respectively.
(3) The activity of β-glucuronidase was low in control group, pure pigment stone cases and calculous hemolytic jaundice cases, as it was high in cholesterol stone cases (at around pH 4.6) and calcium bilirubinate stone cases (at around pH 6.8).
(4) It was revealed that the control specimens contained high concentration of glucaric acid and showed lower activity of β-glucuronidase. In cholesterol stone cases, there was inclined to be estimated less glucaric acid when activity of β-glucuronidase was intense, and vice versa. On the contrary, this mutual relation was not recognized in the specimens of calcium bilirubinate stone cases.
These facts may suggest that glucaric acid-1, 4-lactone estimated as glucaric cid in the present study inhibits activity of β-glucuronidase in bile. Also it is interesting there was relative to the content of glucaric acid and of total bilirubin in control group, but not in calculous cases.
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