Endoscopic pancreatography was carried out on 86 patients with pancreatic and biliary disorders, and function tests of the pancreas, including pancreozymin secretin test, urinary amylase determination and glucose tolerance test, were also done on 62 of them. On the other hand, pancreatography of the isolated pancreas taken off in the autopsy was also made in 31 dead cases. 1)The accessory pancreatic duct was visualized in 60% of post mortem examination and in 40% of clinical cases.2)In the patients whose diameter of the main pancreatic duct were over 4.1mm, abnormal functions of the exocrine pancreas were observed more frequently. 3)In the cases, observed such abnormal findings as tortuosities and distortions of the pancreatic duct, irregularities of caliber, seudodiverticula and parenchymal reflexes in the pancreatography, abnormal function tests were detected more often than another cases. 4)Judging from function tests, irregularity of the caliber might be a most reliable abnormal finding.(p<0.05). 5)Although there were no straight relationship between both morphologic and function tests, remarkable morphologic changes are apt to accompany abnormal functions of the pancreas.
Acid mucopolysaccharides (A-MPS) in the gastric wall of rats were measured by the carbazole method and electrophoresis using two buffers, Ca and Ba acetate. A new method of calculating percentage fractions of A-MPS, i.e. hyarulonic acid (HA), heparitin sulfate (HS), chondroitin sulfate B (ChS-B) and chondroitin sulfate A-k-C (ChS-A+C), by analizing the differences in the bands obtained from two electrophoresis, was devised. Using these methods, changes in the A-MPS of the gastric wall during the course of clamping and clamping-cortisone ulcer were observed. The content of the uronic acid measured by the carbazole method was 56.9μg per 100 mg dry weight of the stomach wall in normal rats. The content of total uronic acid increased 2.5 times in the gastric wall at the 1st week and decreased to the normal level at the 3rd week after the clamping ulcer was made. However, in the C-C ulcer, the increase of the acid was only 1.6 times that of the normal stomach at the 1st week and returned to the normal level at the 3rd week. This fact seems to show that cortisone suppressed a reactive increase of A-MPS in the early stages of the healing of the ulcer and resulted in a delay of healing. By measuring the uronic acid separately in the ulcerated portion and non-ulcerated portions, it was found that the acid content was 1.5 times greater in the ulcerated portion than that of the non-ulcerated portion, in the C-ulcer. However, in the C-C ulcer, no difference between the two portions were observed. The percentage fraction of ChS-B increased 2.3 times at the 1st week in the C-ulcer, but not in the C-C ulcer. The percentage fraction of ChS-B increased remarkably 3.7 times that of the normal level and the other fractions of the A-MPS except HS decreased at the 3rd week. In the C-C ulcer, the percentage fraction of ChS-B increased 5.0 times and all the fractions decreased at the same time. The increase of the ChS-B fraction seemed to be attributed to the increase of the connective tissue in the base of the ulcer.
Nine patients with pancreatic diseases and thirteen patients without pancreatic diseases were subjected to the examination of parotid saliva. The results revealed apparently that the volume flow rate of parotid saliva was decreased, and the maximum bicarbonate concentration and amylase content in parotid saliva were reduced in patients with pancreatic diseases in comparison with patients without pancreatic diseases. The possibility was emphasized that pancreatic diseases might be diagnosed on the basis of changes of volume flow rate, the maximum bicarbonate concentration and amylase content of parotid saliva.