1) Dose responses of acid secretory capacity by AOC-tetragastrin (oil suspension) and insulin (Novo lente) were examined in Schild's rats. Optimal dosis of AOC-tetragastrin and insulin were 1mg per day and 2 u. per day respectively. 2) After administration of AOC-tetragastrin (oil suspension 1mg per day for 21 days) and insulin (Novo lente 2 u. per day for 21 days), 3H-thymidine 250μCi was injected i.p. 30 minutes before killing. Labeling index of generative cells in the fundic mucosa was 28.6% in AOC-tetragastrin administration, 27.2% in insulin and 14-17% in control. 3) AOC-tetragastrin (oil suspension 1mg per day) and insulin (Novo lente 2 u. per day) were administered in rats for 35 days. AOC-tetragastrin caused marked increases in the volume, in the total parietal cell population, ands light increases in the total chief cell polupation of the fundic mucosa. Insulin caused increases in the total chief cell population of the fundic mucosa, but had no effect on the total parietal cell population. In the administration of AOC-tetragastrin together with insulin, the total parietal cell population was very much the same as that in AOC-tetragastrin only, and the total chief cell population was very much the same as that in insulin only.
In order to know the regenerative pattern of gastric ulceration, the following investigations were carried out. The experimental gastric ulcer composed with the normal mucosa on the one side, and with the bases of glands on the other, was made. Regeneration of both sides were histologically compared. At the margins composed with the bases of glands, differentiated cells transformed into undifferentiated cells two days after. The cells changed into undifferentiated foveolar cells 5 days after, and differentiated to matured foveolae 14 days after. At the margins composed with normal mucosa, regenerative cells in the deep part of the newly developed epithelium were closely resembled to the regenerative cells at the margins of the bases of glands. It seemed that the regenerative cells in the deep part of the newly developed epithelium were derived from differentiated cells. On the upper side of the newly developed epithelium at the normal margins, unmatured foveolae appeared 3 days after and matured foveolae developed 5 days after. The regenerative foveolae at the upper side of the newly developed epithelium seemed to be derived from foveolae or undifferentiated cells in the neck region of glands. These findings suggest that gastric mucosal defect was repaired by either differentiated cells in the bases of glands or both the differentiated cells and undifferentiated cells in the neck of glands.
The eight components of serum glycoprotein were determined by single radial immunodiffusion method in 28 patients with cancer of the pancreas, 17 patients with chronic pancreatitis, one patient with acute pancreatitis and 53 patients with non-pancreatic diseases with or without cancer. The results of determination of serum glycoprotein were as follows: 1) The mean concentration of serum α1-antitrypsin and α1-acid-glycoprotein in patients with cancer of the pancreas was significantly higher than that in patients with chronic pancreatitis. The mean concentration of prealbumin and transferrin was moderately lower, but there was no significant alteration of the other glycoprotein fractions. 2) An increase of α1-antitrypsin and α1-acid-glycoprotein fractions, and a decrease of prealbumin and transferrin fractions of the serum in the patients with cancer of the pancreas, liver and biliary tract were noted. These results, however, were not obtained in patients without malignant tumor. 3) Increased α1-antitrypsin and α1-acid-glycoprotein, and decreased prealbumin and transferrin returned to the normal limits following clinical improvement of patients. On the basis of these findings, it was suggested that the variations of serum glycoprotein levels might be resulting from neoplastic diseases and their clinical processes.
Polyacrylamide gel electrophoresis made possible the quantitative fractionation of pepsins in human gastric juice. Human gastric juice pepsins were separated into four major fractions and seven minor fractions by this newly developed method. Fraction 1 had a pH optimum different from fractions 2, 3 and 4. When incubated at pH 3.5, fraction 1 was found to be more stable, thereby suggesting that it may be identical to gastricsin. Little change was observed in the relative proportions of these four major fractions, when the pepsin patterns of gastric juice obtained from normal controls were compared with those from the patients with gastric and duodenal ulcer. It was demonstrated that pepsin patterns obtained from gastric juice, before and after gastrin stimulation, and those from acidified fundic mucosal extracts were essentially similar. Therefore, it is not possible to conclude that each pepsin fraction is secreted from corresponding pepsinogen producing cell.
In many drug-induced allergic hepatitis, peripheral lymphocytes were transformed by the stimulation with a given drug in the presence of autologous serum. However, when rat liver microsome fraction or soluble liver specific antigen fraction was added to the culture instead of autologous serum, the drug-induced lymphocyte transformation was more efficiently shown than autologous serum, while rat liver mitochondria fraction was less effective. On the other hand, in the cases of drug-induced allergic eruption, the addition of liver subcellular fractions were for less effective to induce the lymphocyte transformation than autologous serum. These results may suggest that liver subcellular components involve in pathogenesis of the drug-induced allergic hepatitis.