Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 74 , Issue 6
Showing 1-14 articles out of 14 articles from the selected issue
  • Kouichi ONODERA, Masaru HAYAKAWA
    1977 Volume 74 Issue 6 Pages 709-719
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    The cell kinetics of rat colonic tumors induced by N-methyl-N'-nitro-N -nitrosoguanidine was studied. The autoradiographic examination was not able to distinguish the cancerous sion from the adenomatous one, and also the life span of tumor cells was various. There were some regions with tall crypts in spite of no atypia, and in such regions, thesite of cell division was magnified from the bottom of the crypt near to the surface.
    Considering from our results, the process of tumor occurence was estimated as follows:(1) The site of cell division was growing wide from the bottom of the crypt near to the surface.(2) The crypt grew taller.(3) The small protuberance without atypia was formed.(4) Atypia appeared in the protuberance.
    Download PDF (14069K)
  • Takahiko OHYA
    1977 Volume 74 Issue 6 Pages 720-731
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    In order to clarify the mechanism of hepatic dye uptake, association of ICG with serum proteins, measurement of hepatic ICG uptake in rat and man were investigated. Lipoproteins had a higher affinity for ICG dye than albumin. The ability of hepatocytes to take up dye became rate limiting at very high dye concentrations. Removal rate of ICG in human subject showed the same relationship to the dose administered as was bound in the rat when initial removal rate and dye dosage were plotted according to the Lineweaver-Burk method. Administration of bilirubin decreased the rate of hepatic ICG dye uptake without significantly changing the maximum hepatic uptake of ICG, suggesting competitive inhibition of ICG uptake. Exchange diffusion was demonstrated in studies using the isolated rat liver perfusion and isolated hepatocytes. Unlabeled ICG or BSP added to the extracellular medium, intracellular 35S-ICG was transported out of the cells although the extracellular concentration was higher than the intracellular concentration.
    From these facts, it might be concluded that ICG dye was transported into the liver cells by a membrane mediated system.
    Download PDF (2909K)
  • I. Clinical Investigation
    Toshikazu YOSHIKAWA, Shuhei TAKEMURA, Haruki KATO, Nobuyoshi YOKOE, Mi ...
    1977 Volume 74 Issue 6 Pages 732-739
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    It has already been reported that patients with liver diseases show decreased serum concentration of vitamin E compared to normal. However, the significance of vitamin E in liver diseases has not been clarified.
    One hundred patients with liver diseases were investigated for their serum vitamin E, and it was found that acute hepatitis, fulminant hepatitis, and alcoholic hepatitis showed decrease in vitamin E concentration, while normal in chronic hepatitis, liver cirrhosis, and in hepatic cancer.
    It is interesting that the serum vitamin E level showed good correlation to that ofβlipoprotein, indicating that vitamin E is proportional to theβ-lipoprotein level.
    Attempt was also made to investigate the role of vitamin E on the dissociation of hemolytic complement activity in serum and plasma, reported by Kondo et al. as “cold activation of complement” (J. Immunol., 117: 486, 1976), however.
    Download PDF (814K)
  • with special reference to newly detected two isoenzyme
    Kazumasa MIKI, Hiroshi SUZUKI, Shiro IINO, Hirohumi NIWA, Toshitsugu O ...
    1977 Volume 74 Issue 6 Pages 740-747
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Alkaline phosphatase (ALP) isoenzymes in carcinoma of the stomach were investigated on their enzymological and immunological properties. Carcinoma tissue specimens were obtained surgically from 37 cases of gastric, 14 cases of colonic, and 1 case of esophageal carcinoma. Their ALPs were extracted with n-butyl alcohol by a modification of the method of Morton. The aqueous extract was used for analysis. The results were as follows.
    ALP obtained from gastric carcinoma tissues of 37 patients was separated into 5 bands (ALPa, ALPb, ALPc, ALPd, and ALPe) by polyacrylamide-gel disc electrophoresis. It was concluded that ALPa was similar in its enzymological and immunological properties to hepatic-type ALP, ALPb was similar to placental-type ALP, ALPc to intestinal-type ALP, ALPd to hepatoma ALP (Warnock's variant ALP), and ALPe was similar to fetal intestinaltype ALP except for neuraminidase sensitivity. ALPd appeared in 2 out of 37 cases (5%). ALPe was a newly found isoenzyme, and its electrophoretic mobility was similar to fetal intesinal ALP. ALPe appeared in 1 out of 37 cases (3%). Those ALP isoenzymes (ALPb, ALPd and ALPe) represent an abnormal gene expression of cancer cells in the process of oncodevelopmental differentiation.
    Download PDF (3637K)
  • Kazuo TARAO, Koi SO, Tamaki MOROI, Takao IKEUCHI, Osamu ENDO, [in Japa ...
    1977 Volume 74 Issue 6 Pages 748-754
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Endotoxin was studied by Limulus lysate assay on ascites and plasmas of 29 cirrhotic patients presenting with the ascites and esophageal varices and on plasmas of 17 control cirrhotic patients with only esophageal varices. Of the patients with ascites, endotoxin was positive in 23 (79.3%) of the ascitic fluid, and 22 (75.9%) of the plasma. In contrast, endotoxemia was found in only 23.5%(4 of 17) of the control patients.
    Significant difference was found between the two groups in the incidence of endotoxemia (P<0.01). Of the 29 ascitic fluids 17 showed high titer endotoxin measuring more than 10-3μg/ml. Scintiscanning of liver using198Au showed marked decrease in hepatic uptake in 11 of the 17 endotoxemic cases. The mortality rate (47.8%) in patients with endotoxemia was significantly higher (P<0.05) than that without endotoxemia (16.7%).
    However, no endotoxemic case showed hypotention and only two cases showed a fever of above 37.5°C. The occurrence of endotoxin tolerance is suspected. A long range follow-up studies revealed sustained endotoxemia in the cirrhotic patients.
    Download PDF (763K)
  • Takeshi OKADA
    1977 Volume 74 Issue 6 Pages 755-764
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Serum lysozyme levels of viral hepatitis patients were studied to clarify the clinical significance since the antiviral effect was reported. Lysozyme levels on the 98 cases of various liver diseases were detected by tubidometric method and the relations between the lysozyme level and the incidence of HBs antigen and HBs antibody were studied. There was no correlation between the lysozyme levels and the incidence of HBs antigen in the serum of acute hepatitis patients, however there was some relation between the lysozyme level and HBs antibody titre in some cases. Lysozyme levels of chronic hepatitis and liver cirrhosis were low in HBs antigen positive patients compared to in HBs antigen negative patients. On the cases of acute hepatitis, chronic hepatitis and liver cirrhosis, the lysozyme levels were increased compared with those of control cases. The degree of degenerated hepatic cells, phagocytes and Kupffer cells in the regions of various liver diseases, was related with the lysozyme levels. However, there was no lysozyme activity in the serum of the fulminant hepatitis. Thus, lysozyme level was closely related with the pathogenesis of viral hepatitis and important for the diagnosis of various liver diseases.
    Download PDF (1340K)
  • Hikaru WATANABE, Nobuo HIWATASHI, Shoichi YAMAGATA
    1977 Volume 74 Issue 6 Pages 765-773
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Based on the clinical results in 159 cases of ulcerative colitis experienced in our Department during the years 1954-1975, various clinical aspects such as incidence, laboratory findings, complications, treatment and prognosis have been investigated. From these observations, the following results were obtained.
    1) Ulcerative colitis is not as rare as previously considered. It is slightly female predominant and most often found in adolescents and adults. However, one of the most interesting findings is the secondary increase in incidence in older people, producing a bimodal curve, as described by Evansf & Acheson (1965) and Burch et al.(1969).
    2) In 158 of 159 cases (99.4%), the rectum was affected. Therefore proctosigmoidoscopy, including biopsy and cytologic examination, is important for diagnosis.
    3) Classical ulcerative colitis such as total colitis and left-sided colitis is characterized by mucous bloody stool as its chief symptom and often accompanied by fever, loss of weight, a rise in blood sedimentation rate, anemia, leucocytosis, hypoproteinemia, lowering of A/G ratio and hypokalemia. These findings, except for bloody stool, were not commonly found in proctitis. However, findings of proctosigmoidoscopy, cytologic examination, biopsy and radiological examination between classical ulcerative colitis and proctitis were similar. On the other hand, the lesion progressed to a part or all of the colon in 7 of 38 cases (18.4%) of proctitis during the observation period. Proctitis is, therefore, thought to be a part of ulcerative colitis.
    4) The evaluation of medical treatment discloses that remission was obtained in 21 of 46 cases (45.7%) of total colitis, in 24 of 35 cases (68.6%) of left-sided colits and in 31 of 38 cases (81.6%) of proctitis. Surgical intervention became necessary in 24 of 46 cases (52.2%) of total colitis, in 5 of 29 cases (17.2%) of left-sided colitis and in only one of 31 cases.(3.2%) of proctitis.
    5) Follow up studies revealed the recurrence of symptoms in many cases, although most patients were capable of maintaining normal lives.
    Download PDF (1049K)
  • Eiji SATO, Keiji FUJITA, Takehiko INAGAKI, Kyoichi INOUE, Hiroshi SASA ...
    1977 Volume 74 Issue 6 Pages 774-778
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Nicotinic acid provocation test was performed in three cases of Gilbert's disease and five normal controls.
    Maximum arising amount (maximum value after injection level before injection) of serum total bilirubin was 1.60±0.64mg/dl in Gilbert's disease and 0.52±0.12mg/dl in normal control (p<0.05), thus suggesting that nicotinic acid provocation test was significant procedure for the diagnosis of Gilbert's disease.
    Simultaneous increase in concentration of serum iron was observed in the tested cases, but not found in case with splenectomy.
    It was supposed that increase in serum indirect bilirubin in this test may be related to intrinsic bilirubin load due to hemolysis induced by stimulation of reticuloendothelial system of spleen, bone marrow and liver.
    From the result of nicotinic acid provocation test bilirubin metabolism in hemolytic disease, Dubin Johnson syndrome, Rotor's syndrome and hepatic cirrhosis with indirect hyperbilirubinemia was briefly discussed.
    Download PDF (618K)
  • Yozo IIDA, Susumu KAWAMURA, Kiwamu OKITA, Sumio URAYAMA, Toshihiko TAK ...
    1977 Volume 74 Issue 6 Pages 779-789
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Three cases of primary duodenal cancer and 5 cases of metastatic cancer to the duodenum were reported and analyzed from clinical view points. According to statistical study, the frequency of the cancer in the small intestine is about 3% of the cancer which developed in intestine and over the half of cancers which were seen in small intestine was duodenal cancer.
    Clinical signs and symptomes of the duonenal cancer are not typical at the time of onset, and routine roentgenographic examination of the G-I tract failed to make clear its possibility. Therefore, when its diagnosis was established, already it metastasised to the general.
    Development of duodenal fiberscope is remarkable, never-the-less, diagnosis of early duodenal cancer is still difficult. Therefore, roentgenographic examination should be done not only duodenal bulb but also to the Treitz's ligament, and even the observation of the ampullary region is necessary in endoscopic examination.
    Download PDF (15210K)
  • A Case Report
    Shuhei NOMOTO, Takeshi KITAGAWA, Eiichi IKEBUKURO, Makoto ITO, Tsutomu ...
    1977 Volume 74 Issue 6 Pages 790-794
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Download PDF (2730K)
  • Tsuneo FUKUSHIMA, Kazumasa USUDA, Naoki ISHIGURO, Kiichiro HORI, Kazuh ...
    1977 Volume 74 Issue 6 Pages 795-801
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Download PDF (4797K)
  • Nozomi YAMAGUCHI, Masashi KIMOTO, Hajime OHOMI, Hideki INOUE, Akira AO ...
    1977 Volume 74 Issue 6 Pages 802-808
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
  • 1977 Volume 74 Issue 6 Pages 809-817
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Download PDF (1364K)
  • 1977 Volume 74 Issue 6 Pages 818-847
    Published: June 05, 1977
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    Download PDF (5401K)
feedback
Top