Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 75, Issue 3
Displaying 1-14 of 14 articles from this issue
  • Sotaro FUJIMOTO, Kunihiko KIMOTO, Keiichi KAWAI, Takanori HATTORI, Shi ...
    1978 Volume 75 Issue 3 Pages 257-264
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    On identifying gastrin cell using indirect immunoperoxidase method, authors analyzed the process of tissue specimens before the immunological procedures, and investigated the merits of this method comparing with immunofluorescence.
    Sections stained with indirect immunoperoxidase method can be observed with conventional microscope, and furthermore, adding the poststaining to them, glandular structure in the back-ground becomes much clear, and the autofluorescent cells can be excluded. From these points of view, it can be said that the indirect immunoperoxidase method is more suitable for identifying gastrin cells than the immunofluorescence.
    As to the treatment of tissue specimens, the conventional techniques, i.e. fixation with formalin and embedding into paraffin, is available for obtaining the specific stain to gastrin cells, and this specific stainability of gastrin cell is sufficiently preserved even in a section which was stained with hematoxylin and eosin one year before.
    It can be emphasized that these procedures are convenient and suitable for histological investigations.
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  • A relationship between gastric acid secretion and the time of"healing"
    Hirouki KATO, Toshihiko MATSUMOTO, Yoshio NISIDA, Ituo MIYAZAKI
    1978 Volume 75 Issue 3 Pages 265-271
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The relations between the gastric acid secretion and the time of healing in twenty-two patientswith duodenal ulcers, who were undergone conservative therapies at our out patient departmentduring the period from May, 1974 to December, 1976, were studied for the purpose of predictioningthe prognosis of duodenal ulcer. Following results were obtained:
    1) The time of healing tends to prolong as the values of B.A.O. and M.A.O. (tetragastrin stimulated) increase. Especially, all of the five patients whose B.A.O. values were exceeded 7mEg/h required more than eight weeks for healing.
    2) Patients with a low value of M.A.O. (insulin stimulated) were judged to be healed withinfour weeks. In contrast, no particular tendency in time of healing was observed among patients witha high value of M.A.O..
    3) Recurrence of ulcer was recognised in three patients (14%). The gastric acid secretionbefore treatment was high in one of these patients, but low in two patients especially in B.A.O.. There seemed to be no relationship between the gastric acid secretion and ulcer recurrence.
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  • Toshifumi HIBI
    1978 Volume 75 Issue 3 Pages 272-283
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Immunoblobulin containing cells in the lamina propria of the small intestine or colon, wereinvestigated in various diseases of the intestine with abnormality in serum immunoblobulin. In fourof 7 cases with protein losing gastroenteropathy, IgA containing cells of the jejunal mucosa weredecreased in number. However the decrease of IgA containing cells was not seen in 5 cases withBehcet's disease which might show lymphangiectasia in the small intetine same as protein losingenteropathy. The relationship between IgA containing cells and the formation of lymphangiectasiain the small intestine was not observed. In 13 cases with ulcerative colitis, there were no definitetendency on the number of immunoblobulin containing cells of the colonic mucosa.
    Moreover IgA containing cells in the small intestine of nude mice, which had no or few IgAcontaining cells, were studied before and after human thymus grafting. IgA containing cells weresignificantly increased in number after thymus grafting. These results suggested that thymus mightplay an important role in local immune mechanisms of the gut.
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  • Tadashi MIYOSHI, Takeshi OHHARA, Mikito MORIOKA
    1978 Volume 75 Issue 3 Pages 284-293
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Studies on carcinogenesis and extension of 1, 2-dimethylhydrazine (DMH) induced large-bowel cancer were carried out in 40 rats using a complete autopsy at 220 days from the start of the treatment.
    The histological changes of the colon treated with DMH were divided into 4 lesions by serial sectioning of the entire colons: 1) atypical glands, 2) adenoma, 3) non-invasive cancer, and 4) invasive cancer.
    As many as 86% of colonic adenocarcinomas were originated as de novo cancer, and theremaining 14% arose from adenoma. Carcinogenesis of this model showed higher incidence in theleft half of the colon, and a tendency of multiplicity.
    Histological studies revealed well differentiated adenocarcinoma, poorly differentiatedadenocarcinoma and mucinous carcinoma, with high incidence of peritoneal dissemination andabdominal lymphnode metastasis.
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  • Masakatsu KANO
    1978 Volume 75 Issue 3 Pages 294-305
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Low serum lipids are a feature of the patients with malabsorptive state but no exact data existon the individual plasma lipoproteins in such patients. In this study, the concentration of totalcholesterol, triglyceride and phospholipid of very low (d<1.006), low (1.006<d<1.063), and high(d>1.063) density lipoproteins (VLDL, LDL, HDL) were investigated in fasting plasma from 15healthy control subjects and 9 patients with various kinds of secondary malabsorption.
    Patients with malabsorption comfirmed by 131I-triolein test, had lower level of cholesterol inLDL and HDL, which were 59.5% and 71.6% of those from healthy control subjects. Phospholipidsin LDL and HDL were also decreased to 92.0% and 71.2% of the controls. Calculation of the molarratio of cholesterol: triglycerides: phospholipids revealed a major difference between healthy controlsand patients, but VLDL composition remained almost unchanged. Another striking finding was adecreased percentage of lionleate (C18:2) in all lipoprotein fatty acids, especially in cholesterolestersof LDL and HDL. These changes in LDL were proportional to the degree of malabsorption andreflect on the changes in whole plasma, irrespective of whether they were due to the defect of intraluminaldigestive procedure or of intestinal absorption. High levels of triglyceride in VLDL implycarbohydrate induction, presumably due to relatively less severe malabsorption of carbohydrate thanthat of fat.
    These results suggest that LDL lipids can be extensively modified by impaired absorpion ofcholesterol and long chain fatty acids, and that fatty acids in lipoproteins at least partially be inducedfrom carbohydrate metabolism in the patients with secondary malabsorption.
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  • Norio KOBAYASHI
    1978 Volume 75 Issue 3 Pages 306-314
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Binding of indocyanine green (ICG) to human serum protein was investigated using by gel filtration, electrophoresis and ultracentrifugation.
    ICG bound to α-lipoprotein (α-Lp), β-lipoprotein (β-Lp), and albumin (Alb) and α2-globulin(α2Gl) of human serum. At low concentration of ICG in serum (1×10-5M) it bound obviously to β-Lp. At high concentration (1×10-3M) it bound to β-Lp, α-Lp, and Alb and α2Gl. There were aproportional relation between binding of ICG to each protein and ICG concentration in serum.
    Binding of ICG to serum protein revealed two groups of binding sites by Rosethal plot withstepwise elution method. One was a high-affinity and low-capacity, and the other was a low-affinityand high-capacity. The binding of sulfobromophthalein to human serum revealed one group ofbinding site as high-affinity and low-capacity.
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  • Teisuke KAMATA, Kenzo KOBAYASHI, Mitsuru HATAYAMA
    1978 Volume 75 Issue 3 Pages 315-323
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    In order to make clear pathophysiology of the bleeding in the gastrointestinal tract in patientswith chronic hepatic disease, the macroscopic and histological findings of the mucosa of thealimentary tract of autopsied cases with chronic hepatic disease were examined. The results weresummarised as follows.:
    1) Gastrointestinal bleeding in 29 (81%) out of 36 autopsied cases was revealed clinically, and 14 cases (39%) of them were fatal for its bleeding and 15 cases (42%) of them were not fatal for it.
    2) Esophageal varices and congestion and petechia on the mucosa of alimentary tract wereclosely related to gastrointestinal bleeding macroscopically, especially congestion was observed athighest rate (90%) on gastric mucosa.
    3) Significant changes were observed in the mucosa of the gastrointestinal tract histologically.
    a) The vein in the submucosal layer of the esophagus was dilated remarkably, and the thickness and the elasticity of its wall were not even.
    b) The capillary in the mucosal layer from esophagus to rectum was much proliferated and dilated, especially in the gastric mucosa.
    These findings suggested that the gastric mucosa were seemed to be the most important bleeding source in patients with chronic hepatic disease.
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  • Tsuneaki ARISHIMA
    1978 Volume 75 Issue 3 Pages 324-336
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Clinical significance of serum GOT and its isoenzymes and GPT was studied in hepatocellularcarcinoma comparing with those of patients with acute and chronic liver disease. In addition, human liver and hepatoma tissue, obtained during surgical operation and postmortem autopsy wereexamined for activities of those enzymes. Experimental hepatoma, induced by 3' -Me-DAB in rats, was also studied in the same way as in human, (GOT isoenzymes were separated by immunoadsorbentmethod with specific antibody to supernatant GOT.)
    The results in human cases were as follows; (1) elevation of serum GOT activity appears earlierthan that of GPT with increase of GOT/GPT ratio through the clinical course, (2) incleasedmitochondrial GOT level appearing in serum was suggestive of severity of liver injury in nonhepatomaand hepatoma liver, (3) serum mitochondrial GOT was lesser than 20% of the total serum GOT activity in hepatoma, (4) tissue activities of the enzymes were decreased either in hepatoma ornon-hepatoma portion of the liver and decrease of GPT activity was especially predominat inhepatoma tissue.
    Similar results were obtained in experimentally induced hepatoma; (1) serum GOT activity washigher than GPT activity with increased GOT/GPT ratio approximately 3 fold, (2) activities of theisoenzymes in hepatoma tissue were reduced, especially GOT of the supernatant fraction.
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  • Analyses of Anatomical Pattern and Rigidity and Dilatation of the System as Pathological features
    Hirohiko YAMASE
    1978 Volume 75 Issue 3 Pages 337-349
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    For the purpose of analysing the patterns of cholangiographic changes in various pathological conditions and correlating them with each other, study was made in 52 autopsy and 483 clinical materials. Stereographic technic was employed in 100 of these 535 cases.
    1) Stereographic technic was particularly useful in delineating intrahepatic biliary system, and might well be a valuable adjunct for clinical purposes.
    2) There was a great variation in the pattern of intrahepatic biliary system in the right lobe in particulary. Based on the mode of the confluence of the first to the third order radicals mainly of the right side hepatic ducts, no fewer than 35 types of variation were counted in the pattern of the main intrahepatic bile ducts, in an analysis of 100 cases. The mode of the junction of the right and left hepatic ducts could be divided basically into 6 types.
    3) The degrees of rigidity of the duct contour observed in cholangiograms and thickening of the duct wall in the microscopical examinations were in close correlation.
    4) Rigidity of the bile duct was considered to be essentially indicative of the inflammatory process exsisting in the biliary system. Therefore, this finding in cholangiograms might well be a useful parameter in the differential diagnosis of various disease processes in the biliary system.
    5) It was also suggested that dilatation of the intrahepatic bile duct was mainly dependent on the degree and duration of the obstructive process, but that the role played by co-existing thickening of the duct wall might be as important.
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  • Hideoki NOSHI, Tadasu TSUJII, Hiroshi FUKUI, Tsutomu MATSUI, Yoshiaki ...
    1978 Volume 75 Issue 3 Pages 350-358
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    It was studied what influence chenodeoxycholic acid (CDCA) has on the metabolism of cholesterol and bile acids in squirrel monkeys fed on cholesterol gallstone formation diet (Sucrose+Butter+Cholesterol).
    In squirrel monkeys fed on cholesterol gallstone formation diet, cholesterol concentration in bile and liver increased in spite of decrease of hepatic HMG-CoA reductase activity, and bile acids concentration in bile decreased with low activity of 7α-hydroxylase. Such metabolic pattern produced lithogenic bile.
    Addition of CDCA on cholesterol gallstone formation diet inhibited HMG-CoA reductase moreover, and decreased hepatic free cholesterol and biliary cholesterol concentration. On the other hand, 7α-hydroxylase activity was also inhibited by CDCA, but biliary bile acids concentration increased.
    Finally, lithogenicity of bile in squirrel monkeys fed on cholesterol gallstone formation diet was improved by administration of CDCA.
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  • Makoto OTSUKI, Choitsu SAKAMOTO, Mitsuo MAEDA, Kuniyasu OKANO, Hosai Y ...
    1978 Volume 75 Issue 3 Pages 359-365
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The effect of intraduodenally infused 1-phenyl-1-hydroxy-n-pentane (PHP) on portal venous plasma secretin concentrations, pancreatic flow rate and amylase output was investigated simultaneously on pentobarbital anestheized rats. Moreover, the effect of exogenously administered somatostatin (GIF) on PHP-stimulated secretions of secretin and the exocrine pancreas was studied.
    Portal venous immunoreactive secretin (IRS) concentration rose from 78.8±4.5pg/ml before to 335.4±68.9pg/ml 5min after initiation of the infusion of PHP 200mg/kg/min, while insignificant changes in the plasma IRS levels were observed following infusion of PHP 50mg/kg/min. However, the responses of the exocrine pancreas to these different doses of PHP were near the same.
    Although administration of GIF (5μg/kg/h) almost completely suppressed 200mg/kg/min PHP-stimulated release of secretin, pancreatic flow rate and amylase secretions were only partially inhibited.
    These results indicate that the stimulation of pancreatic exocrine function by PHP is a complex phenomenon involving the release of secretin and other unidentified factors which have the stimulatory effects of pancreatic secretion; PHP acts as a weak stimulant for the release of secretin but a strong stimulant for the release of other factors.
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  • S. INOUE, H. MASUDA, [in Japanese], [in Japanese], [in Japanese], [in ...
    1978 Volume 75 Issue 3 Pages 366-373
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    A case of Adenomyoma of the gallbladder (fundal type) with gastric polyps is reported. Case, 61 year-old female was admitted to Akita University Hospital because of precise examination of gastric polyp, which was detected in the mass survey of the stomach. Gastric polyps were located on the anterior wall of the antrum. Biopsy specimens under direct vision showed the hyperplastic polyp. Cholecystography demonstrated a minute filling defect on the fundus of the gallbladder. A nodular lesion without central ulceration on the fundus of the gallbladder and Gastric polyps were found in the excised specimen. Histologically, irregular tubular formation of mucosa and hyperplasia of muscular fibers were observed.
    There were many terminologies and classifications for non-inflammatory lesions of the gallbladder. But, adenomyoma of the gallbladder is a comparative rare lesion and previously reported literatures are reviewed.
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  • Yasuni NAKANUMA, Toshihisa SHINTANI, Goroku OHTA, Mikio KUMAGAYA
    1978 Volume 75 Issue 3 Pages 374-380
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    A 64 year-old woman of primary biliary cirrhosis with abnormal intrahepatic portal vein was reported. A slight varicosis of the esophagus and cardia of the stomach was found 6 months after her initial symptoms of pruritus and jaundice and she died of massive G-I bleeding due to portal hypertension two and a half years after her initial symptoms. Surgical liver biopsy (early stage) and autopsy (middle stage) showed abnormal changes of intrahepatic portal vein: luminal narrowing, thickened wall of portal vein composed of fibroelastosis and hyperplasia of smooth muscles. These changes of the intrahepatic portal vein seemed to be associated with the appearance of portal hypertension in this case.
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  • Michiya NAKAJIMA, Muneharu NAKAYAMA, Hiroshi SAITO, Hirokichi SASAKI, ...
    1978 Volume 75 Issue 3 Pages 381-385
    Published: 1978
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Multiseptate gallbladder is an extremely rare congenital anomaly, the number of reported cases not having exceeded ten throughout the world. This is the account of a 37 year old man with this condition who had had a long history of occasional nausea, vomit and abdominal pain. Intravenous cholecystography demonstrated a gallbladder with an irregularly outline, and tomography disclosed thin septa inside. The surgically removed gallbladder looked like a honeycomb on the cut surface. The bile was whitish in the fundus and dark brown in the neck, suggesting disturbed bladder function. Histologically, muscular layers in the partitions were continuous from the gallbladdeer wall. He has been free of the past complaints after surgery which were attributable to dyskinesia of the gallbladder.
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