Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 76 , Issue 10
Showing 1-17 articles out of 17 articles from the selected issue
  • Kazumasa MIKI, Hiroshi SUZUKI, Hirohumi NIWA, Shiro IINO, Jun MIYAZAKI ...
    1979 Volume 76 Issue 10 Pages 1921-1928
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Alkaline phosphatase (ALP) zymograms from rat gastric carcinoma tissues showed a broad single active band by 5 % polyacrylamide-gel disc electrophoresis, however, after treatment with neuraminidase, they were divided into 2 sharp active bands, A band (Rf=19%) and B band (Rf=32%). Enzymologically and immunologically, B band showed similar properties to those of rat intestinal ALP isozyme, but A band had different ones.
    ALP siozymes in the glandular stomachs of Wistar strain rat experimental intestinal metaplasia and carcinoma induced by N-methyl-N'-nitro-N-nitrosoguanidine at a concentration of 83 μg/ml in the drinking water for 17 weeks, were studied.
    Ten of 13 cases (77%) of ALP zymogram from the glandular mucosa of stomach showed B and only and the other 3 cases (23%) showed A band only.
    The ALP activity of one carcinoma tissue showed 100 times higher than that of the surrounding glandular mucosa. We proposed the possibility that rat experimental cancer cells produced extraordinary high amount of ALP is due to the one of the abnormal gene expressions of the cancer cells.
    We suggested that this rat experimental system will be a useful approach for the study of the development of intestinal metaplasia and gastric carcinoma in human stomach
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  • Keiichiro KONDO
    1979 Volume 76 Issue 10 Pages 1929-1941
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Sixty-two patients with gastric ulcer (GU) and 59 patients with duodenal ulcer (DU) were each divided into two groups according to the values for MAO to histalog stimulation; normosecretor with a MAO below 20 mEq/h and hypersecretor with a MAO above 20 mEq/h. Following investigations were executed on these patients. The gastric acid response to insulin hypoglycemia was determined after an intravenous administration of 10 U of regular insulin. The serum gastrin responses to insulin and meat extract were measured by radioimmunoassay using CIS-gastrin kit. The tissue gastrin concentration in the mucosa of pyloric gland area was also measured by radioimmunoassay. The evidences of gastritis accompanying with GU or DU were observed microscopically. Based on these studies, the differences in clinical and physiological aspects between patients with GU and DU were definitely analysed.
    Functioning parietal cell mass (FPCM), functioning G-cell mass (FGCM) and vagal capacity were all increased in hypersecretor of DU. FGCM and vagal capacity were also increased in normosecretor of DU, but FPCM was decreased in number in this group. The increases of FPCM and vagal capacity were observed even in hypersecretor of GU, whereas FGCM showed only a slight increase, comparing to that of normosecretor of GU. In normosecretor of GU, FPCM was decreased, and vagal capacity was significantly limited. From these results, it was noted that a part of patients with GU has similar clinical features with patients of DU. The incidence in all the patients with GU was about 30 per cent. Accordingly, the treatment for hypersecretor of GU should be considered in a different manner from that for normosecretor of GU.
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  • Yoshiaki HORIE
    1979 Volume 76 Issue 10 Pages 1942-1954
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Recently it has been noticed the organic microcirculatory disturbance in the bowel wall may result in the ischemic intestinal lesions. To clarify the pathophysiology of the ischemic lesions due to minute vessels occlusion, microbarium suspension was injected into the mesenteric vessels of the mongrel dogs. The degree of the bowel damage studied histologically was nearly parallel to the injected amount of microbarium suspension. The sigmoidoscopic examination was performed in some of the dogs.
    With the small amount of barium suspension, the mucosal surface became uneven and protrusive, accompanied with hemorrhage and superficial necrosis. With one or two weeks, these reactive changes subsided gradually. As these lesions were confined only in the mucosal layers, the pathological process were transient and reversible. When the ischemic changes involved the submucosal or muscular layers beyond the bases of crypts, the ulceration developed later, followed by high incidence of the stenosis due to scar formation with severe fibrosis within two weeks. If the injected amount of barium suspension was large, the total layers of the bowel wall became necrotic within a few days, and the dogs died rather rapidly due to the intestinal perforation. In electronmicroscopic study, the changes of the intestinal mucosal epithelium appeared distinctly in 30 minutes after barium injection.
    From the results of our experimental studies, we concluded that the minute vessels occlusion promoted a series of the ischemic changes such as hemorrhage, ulceration and necrosis of the bowel wall, the severity of these changes being propotional to the degree and extent of the occlusion.
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  • Kazuaki KMISAKA, Masanobu TSURU, Masanori HIRANO, Tooru INAGAKI, Kenic ...
    1979 Volume 76 Issue 10 Pages 1955-1959
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Human ligandin and acidic Z protein concentrations were quantitated by radioimmunoassay in sera and liver supernatant obtained from controls and patients with liver diseases. Serum Z protein values were statistically correlated with serum ligandin in patients with liver cirrhosis (r=0.57 p<0.01)(39 samples), but not in acute hepatitis (r=0.35)(23 samples) and chronic hepatitis (r=0.197)(37 samples). Serum concentrations of Z protein were all decreased in hyperbilirubinemic patients (Gilbert's Syndrome 5 pts., Dubin Johnson Syndrome 2 pts., Rotor hyperbilirubinemia 1 pts.). Liver ligandin and Z protein concentrations were decreased generally in various liver diseases.
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  • 3. Comparison with Chang liver cell as a target cell and studies on the surface marker of cytotoxic lymphocyte
    Toshio MORIZANE, Kanji TSUCHIMOTO, Tetsu WATANABE, Akijiro OHTSUKA, Ke ...
    1979 Volume 76 Issue 10 Pages 1960-1969
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The cytotoxicity of peripheral blood lymphocytes against Chang liver cell and LiC-l (Ohtsuka) was studied by microcytotoxicity assay using separated lymphocyte subpopulations of E rosette forming cells and non E rosette forming cells. Cell surface markers of cytotoxic lymphocytes against these target cells were studied by direct immunofluorescence method.
    T cell depleted population of the peripheral blood lymphocytes exhibited the strong cytotoxic activity against Chang liver cell in patients with chronic active hepatitis and liver cirrhosis. The lymphocytes from healthy donors showed only slight cytotoxicity against Chang liver cell. The lymphocytes in the process of cytotoxicity against Chang liver cell in vitro showed Fc receptor on cell surface which was detected by FITC labeled human heat aggregated IgG, but not surface Ig by FITC labeled anti-human Ig goat antibody. This effector against Chang liver cell was considered to be mainly "K cell".
    The cytotoxicity of peripheral blood lymphocytes from healthy donor against Chang liver cell was enhanced by addition of sera from patients with chronic active hepatitis. This cytotoxicity was mediated by the lymphocytes which belonged to T cell depleted population and exhibited Fc receptor on cell surface but not surface Ig. This effector was con- sidered to be mainly "K cell".
    The statistically significant difference was not observed in the cytotoxicity against LiC-1 between healthy controls and patients with liver cirrhosis or hepatoma. The cytotoxicity against LiC-1 was mediated by the lymphocytes which belonged to T cell depleted population and exhibited Fc receptor on cell surface and some of which exhibited surface Ig also. This effector was considered to be mainly "natural killer cell (NK cell) ".
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  • Yasuni NAKANUMA, Goroku OHTA
    1979 Volume 76 Issue 10 Pages 1970-1978
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Among 171autopsy cases of infants there were 11 cases with polycystic kidney, and 6 cases with anomalous intrahepatic bile ducts. All three cases with type I polycystic kidney (Potter) and 2 of 5 cases with type II polycystic kidney showed anomalous intrahepatic bile ducts. Five of 6 infants with anomalous intrahepatic bile ducts showed polycystic kidney. Therefore, it seemed likely that anomalous intrahepatic bile ducts and polycystic kidney were intimately associated in infants.
    Three cases with type I polycystic kidney showed proliferation and tortuosity of the dilated intrahepatic bile ducts, while in 2 cases with type II polycystic kidney proliferated and tortuous intrahepatic bile ducts were not dilated and rather transformed into the cordlike structure. This finding and other morphological differences in the liver of two types of polycystic kidneys suggested that morphological and morphogenetical differences might be present in the intrahepatic bile duct lesions between in infants with type I and those with type II polycystic kidney.
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  • Masuyoshi CHO
    1979 Volume 76 Issue 10 Pages 1979-1992
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The dissolving phenomenon of human cholesterol gallstones composed of more than 80% cholesterol was studied by using scanning electron microscope (SEM). Gallstones were divided into two groups, that is, a control group in which gallstones from 3 patients were incubated in beagle bile and another group which consisted of gallstones from 11 patients treated with chenodeoxycholic acid (CDCA).
    The incubation of gallstones in beagle bile reduced 16.5-39.4% of their weight. Comparing with the SEM features of the preincubated gallstones the most remarkable change after incubation was decrease in the number of small crystalline lumps. The facets of these platy crystals were waveshaped, their edges were irregularly shaped and their corners were rounded off. Still more, laminary plates were piled up closely before incubation, but after it, hollow gaps appeared between each plate. These changes were observed in both of the outer surface and the core and considered to result from the dissolution of the cholesterol crystals by beagle bile.
    The SEM features of the gallstones from the patients treated with CDCA were identical to those of the control group and this suggests the process of dissolution of human cholesterol gallstoens by CDCA.
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  • Yutaka INAGAKI, Yasuyuki TANAKA, Taro TAKAMURA, Kenji SAKAKIBARA, Nobu ...
    1979 Volume 76 Issue 10 Pages 1993-2003
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Serum CPK level was examined in 5 cases of acute pancreatitis and 4 cases of relapsing pancreatitis who were admitted to Chukyo Hospital during the past one year. An elevation in serum CPK level was found in 4 cases of acute pancreatitis and in 2 cases of relapsing pancreatitis. The degree and duration of the CPK elevation seemed to be related to the seriousness of the disease. The greater part of CPK isozyme was occupied by MM component, and there were no abnormal patterns on ECG compatible with acute myocardial infarction. These findings suggest that the origin of CPK in pancreatitis is retroperitoneal muscles and/or diaphragm. Following possibilities were considered as the etiologic factors of CPK elevation in pancreatitis:
    1) Muscular digestion and necrosis by pancreatic juice.
    2) Muscular microemboli due to local or disseminated intravascular clotting.
    3) Hyperpermeability of fascia and muscular cell membrane due to peripancreatic inflammation.
    4) Compression to muscles by hematoma or exudate.
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  • Toshinari KIMURA
    1979 Volume 76 Issue 10 Pages 2004-2016
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to develop a new enzymatic diagnoses of pancreatic diseases, elastolytic activity (elastase activity) in the duodenal juice was assayed using the specific synthetic substrate, and serum elastase l concentration was measured by radioimmunoassay.
    The revised method using N-succinyl-(L-alanine)3-P-nitroanilide (SUC-(Ala)i-NA) as a synthetic substrate was shown to be more sensitive and useful than those using N-benzoyl-L-alanine-methyl ester (BAME) in determining elastase activity of the duodenal juice. The changes in the elastase activity were demonstrated to parallel with those of the amylase concentration in normal healthy subjects during the pancreozymin-secretin test (PS test) and a high correlation between the elastase output and the amylase output (P<0.001) was observed. Six out of 14 patients with questionable chronic pancreatitis, with normal results from the current PS test, showed definitely low elastase output. In 4 of the 6 cases, endoscopic retrograde pancreatography (ERP) and operative procedures were performed, resulting in definite findings of chronic pancreatitis.
    Serum elastase l concentrations were significantly high in relapsing acute pancreatitis, chronic relapsing pancreatitis and in the early stage of pancreatic cancer, although serum amylase concentrations were only slightly high or were normal. In contrast, serum elastase l concentrations in calcifying chronic pancreatitis and in the terminal stage of pancreatic cancer were significantly low, whereas the serum amylase concentrations were within normal range.
    These results suggest that patients with chronic pancreatitis tend to have more severe impairment in the secretion of elastase than of amylase in the PS test and that the measurement of serum elastase l offers a more specific and sensitive parameter of pancreatic function than does amylase estimation.
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  • Katsuhiro ENDO, Masaru KOIZUMI, Yoshihiko KANAZAWA, Shin YOSHIDA, Mori ...
    1979 Volume 76 Issue 10 Pages 2017-2023
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We have designed four lumen tube which have enabled us to aspirate duodenal juice infusing endogenous stimuli into the lower part of the duodenum. Using this tube, exocrine pancreatic secretion and immunoreactive secretin (IRS) level have been simultaneously observed in response to intraduodenal infusion of l-phenyl-l-hydroxy-n-pentane (PHP) and 0.1N HCl in normal subjects, patients with diabetes mellitus, and patients with chronic pancreatitis.
    1) Pancreatic flow rate and bicarbonate concentration have increased as two times as those of basal level after infusion of PHP or HCl in normal subjects. IRS levels have also increased correspondently in normal subjects. The same tendency have been observed in patients with diabetes mellitus.
    2) Exocrine pancreatic secretion have not changed after infusion of PHP or HCl in patients with chronic pancreatitis. Pancreatic flow rate, bicarbonate concentration and amylase output have been significant low in patients with chronic pancreatitis compared with those of normal subjects and patients with diabetes mellitus.
    3) Pancreatic flow rate and bicarbonate concentration have increased as about two times as those of basal level after both PHP and HCl infusion. On the other hand, although pancreatic amylase output has increased significantly after infusion of HC1 compared with basal secretion, it has not increased after infusion of PHP. Therefore we have concluded that PHP has been more specific substance for releasing secretin than HCl.
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  • III. A new ultrasonic aspiration biopsy transducer with electronic linear scan ultrasonotomograph
    Takeo YAMANAKA, Yukio YOSHIDA, Ken KIMURA
    1979 Volume 76 Issue 10 Pages 2024-2030
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Ultrasonically guided percutaneous fine needle aspiration biopsy for cytological diagnosis by a new ultrasonic transducer with electrolinear scanning ultrasonic diagnostic equipment was performed on patients with pancreatic cancer.
    This new ultrasonic aspiration biopsy transducer has made this procedure more simple and safe. This is because the image of the new ultrasonic real time transducer is dynamic and persistent, permitting actual time observation of physiological movements i.e., respiratory displacement and needle movement.
    On the other hand, an ultrasonic aspiration biopsy transducer with contact compound scan ultrasonotomograph was useful for a small target because of its clear image resolution, but it is not suitable for mobile targets.
    In regard to usefulness on the percutaneous aspiration biopsy of the pancreas, at present, there is no absolute superiority between the two kinds of ultrasonic aspiration biopsy transducer. It is rational that these two ultrasonic aspiration biopsy transducers should be used properly, according to the status and to the extent of mass lesion of each patient.
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  • Shigeru MORICHIKA, Koichi NAGATA, Takashi TANIGAWA, Masanobu IMAI, Hit ...
    1979 Volume 76 Issue 10 Pages 2031-2037
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hiroo ONISHI, Masaru SHIMIZU, Teruo TAKAI, Yasuhiko KAWADE, Kazuo AMAN ...
    1979 Volume 76 Issue 10 Pages 2038-2043
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Fulminant hepatitis due to the massive hepatic necrosis is the most serious syndrome in hepatic inflammations, exhibiting various complications such as cerebral edema, renal dysfunction and impaired blood coagulation system. It is usually difficult to examine the liver histologically during hepatic coma. In this paper, the histological changes in the liver both during and after recovery from fulminant hepatitis were described in a 59 years old woman. On the liver specimen obtained at the initial phase of coma, normal hepatic structure was diffusely destroyed, showing hepatic cell necrosis, degeneration, bleeding and inflammatory cell infiltration. Chronic hepatitis i.e. destruction of the limiting plates, and inflammatory cell infiltration was revealed on two liver biopsy specimens obtained after two and six months, respectively.
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  • Shozo UCHIYAMA, Takako MIZUNO, Yoshiko SAMESHIMA, Takaya TANAKA, Yoshi ...
    1979 Volume 76 Issue 10 Pages 2044-2051
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The report was made on a case of 33 year-old man with multi-hormone producing insulinoma. One of his sisters had a case of WDHA syndrome which could suggested his insulinoma to be a hereditary type.
    The tumor found in the pancreatic tail using abdominal ultrasound, ERCP and angiography was diagnosed as an insulinoma according to Whipple's Triad as well as with giucagon test.
    Mesurement of many hormones such as insulin, glucagon, VIP, ACTH, andβ-MSH in the tumor verified it as multi-hormone producing APUDoma.
    Additional concepts concerning the central nervous disturbance induced by hypoglycemia were discussed in ad hoc literatures.
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  • Osamu NAKAZAWA, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1979 Volume 76 Issue 10 Pages 2052
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Masashi IHORI, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1979 Volume 76 Issue 10 Pages 2053
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Kazuyuki NAGATA, [in Japanese], [in Japanese], [in Japanese], [in Japa ...
    1979 Volume 76 Issue 10 Pages 2054
    Published: October 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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