Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 76 , Issue 6
Showing 1-15 articles out of 15 articles from the selected issue
  • Haruyuki SHINOZAKI
    1979 Volume 76 Issue 6 Pages 1219-1234
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Few studies have been made on the regeneration of the mucosa in the duodenal ulcer, particularly on the role of lamina propria in the healing process of the ulcer. In this paper, the results of the histological and histochemical studies on the regeneration of the mucosa in duodenal ulcers are presented. Duodenal ulcers were produced by the clamping and clamping cortisone method devised by Umehara and Tabayashi. Sprangue-Dawley male rats weighing 200g were used. After keeping without food for 24 hours, the bulb of the duodenum was exposed and clamped with a metal plate, 8×4mm in size. The procedure for making this ulcer was almost the same as that for making gastric ulcer by clamping and clamping-cortisone method. The rats were killed on the first and third ulcer days, the first ulcer week and thereafter every week for eight weeks.
    The bulb of the duodenum was removed and histological preparations were made by routine procedures. The preparation were stained with H.E. for histological examination, and with Azo-dye for studying alkaline phosphatase in the tissue.
    The results are summarized as follows: The epithelium in the marginal zone of the ulcer formed an immature epithelial layer from the first ulcer day and began to extend toward the base of the ulcer. As the epithelial layer matured, regeneration of the villi and Lieberkuhns' gland followed. Thus, the duodenal ulcers made by the clamping method (acute ulcer) healed almost completely within 4 or 5 weeks. The capillaries, which were stained red by the Azo-dye method, developed from the lamina propria of the marginal mucosa and extended to the base of the ulcer, accompanying the regeneration of the epithelium. It seems that there is a close relationship between the regeneration of the capillaries from the lamina propria and of the mucosal epithelium. In the animals treated with cortisone, the healing process progressed more slowly in comparison with that of the clamping ulcer group. In the animals in which a retardation of the healing appeared, hyperproliferation of the connective tissues and inflammation were seen at the base of the ulcer. Because of these pathological changes of the base of the ulcer, it seemed that desquamation of the epithelium occurred frequently in those animals. In conclusion, it was proved that the development of the blood vessels and the perivascular connective tissues of the lamina propria plays an important role in the healing of the duodenal ulcer; that hyperproliferation of granulation tissue of the base of the ulcer may inhibit the healing of the ulcer; and that the clamping and clamping-cortisone methods are very useful for studying not only the gastric ulcer, but also the duodenal ulcer.
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  • Yukihiko NARUKI, Minoru NAKANO, Sachio OHTSUKA
    1979 Volume 76 Issue 6 Pages 1235-1245
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The factors elevating blood CEA levels were examined correlating with the histological findings in 102 cases with gastric cancer. The blood CEA levels showed higher in proportion to the classification of Borrmann's IV, III, II and I type. The positive percent of blood CEA levels in early gastric cancer was 20% regardless of its histological types. In advanced cancer, however, blood CEA levels were influenced by the degree of differentiation, being high in well differentiated types and low in poorly differentiated types. The blood CEA levels became higher as the cancer invaded deeper, but it was not correlated with the presence of metastasis to regional lymph nodes. About the infiltration type (INF), the positive rate of blood CEA became higher in proportion to INF γ, β and α. The positive rate of blood CEA increased according to the progression of the invasion to lymph-vessels and veins. Especially, in the cases of moderately differentiated tubular adenocarcinoma, the blood CEA levels showed high according to vein invasion. Vascular invasion was one of the major factors of CEA passing from the tissue into the blood.
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  • Nozomi YAMAGUCHI, Hideki INOUE, Hiromasa OGAWA, Hajime OHOMI, Kunihiko ...
    1979 Volume 76 Issue 6 Pages 1246-1251
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Serum CEA concentrations were measured in healthy persons and in patients with both malignant and non-malignant diseases by means of a radioimmunoassay utilizing commercial CEA kit (France, CIS-CEA kit). This study is summarized as follows:
    1. The radioimmunoassay kit for CEA measurement using the double antibody technique (CIS-CEA kit) is more useful than other commercial CEA kits at all points of sampling, techniques, cost and reappearance. In our laboratory, carcinoembryonic antigen levels of 0-20 ng per milliliter were considered normal and levels higher than 20 ng per milliliter abnormal.
    2. It is important to bear in mind that the CEA assay is non-specific and elevated levels are occasionally observed in the benign diseases such as inflammatory diseases of the intestine and liver as well as in malignant neoplasms. In this study, the percentage of patients with malignant disorders with an increase in serum CEA concentration was 37%, and the incidence of high serum CEA levels in benign diseases was 9%. In malignant disorders, pancreatic cancer and colorectal carcinoma showed markedly high incidence of increasing in serum CEA concentration.
    3. It is difficult to detect early cancers by means of a radioimmunoassay for CEA. However, the most valuable aspect of CEA measurement at present appears to be in the monitoring of therapy and clinical course. Serial CEA measurements were perhaps of some value in predicting progression of malignant diseases. The levels of CEA may relate to the extent of the malignant neoplasms.
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  • Norio MATSUKURA
    1979 Volume 76 Issue 6 Pages 1253-1258
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Sequential studies on intestinal metaplasia in the glandular stomach of rats induced by N-propyl-N'-nitro-N-nitrosoguanidine(PNNG) were performed. PNNG was given to 56 rats in their drinking water at the level of 100 μg/ml for 44 weeks, and then, tap water until the termination of the experiment. The rats were killed periodically to examine the intestinal metaplasia and tumor in the glandular stomach. The intestinal metaplasia was first found at the 19th week in the glandular stomach of a rat and the incidence of intestinal metaplasia was 57% in the rats killed during the periods between the 19th-28th week. The incidence increased according to the experimental days and 100% of the rats had intestinal metaplasia at the 88th week. Carcinoma in the glandular stomach was first found at the 69th week. There was a great difference in the time of appearance between intestinal metaplasia and gastric carcinoma. In the control rats, intestinal metaplasia was first found at the 50th week and thereafter 20-40% of the rats had intestinal metaplasia. No gastric tumor was fcund in the control rats.
    Metaplastic glands were observed by transmission electron-microscopy and autoradiography. Metaplastic glands showed irregularly developed microvilli and goblet cells with pale mucin granules by electron-microscopy. Autoradiography showed labeled nuclei at the lower half of the metaplastic glands like doudenal epithelium.
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  • Ultrastructual findings of the Colon Surface in the Early Lesion
    Shigekazu HAYASHI
    1979 Volume 76 Issue 6 Pages 1259-1270
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Ulcerative lesions of the large intestines of rabbits were induced by 1% amylopectin sulphate which was given with drinking water. Observation of the intestine was begun the day after the initiation of amylopectin sulphate administration. Even in the very early stage when there were no endoscopic and macroscopic findings, a few positive findings were seen in all specimens by means of the dissecting microscope and the scanning electron microscope. Early dissecting microscopic pictures showed irregular and subdivided colonic areas. Following that, punctuate and linear erosion appeared and ulceration developed. Pictures of early lesions taken with the scanning electron microscope revealed cell adhesion surrounding cryptal openings. Next destructive degeneration of these cells and crypts took place. Early changes of the microvilli were observed as fine hairlike projections, ballooning polypoid shapes and a chrysanthemum-like blooming. Following that, microvilli became sparse with deformity and gradually disappeared.
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  • Tadashi KANOH, Haruto UCHINO, Hiroo FURUKAWA
    1979 Volume 76 Issue 6 Pages 1271-1276
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In recent years the immunological aspects of the gastrointestinal tract have received much attention. Immunological mechanisms at the mucosal surface can operate as protecting the milieu ixtérieur from the milieu ixtérieur. These special mechanisms are concerned especially with secretory IgA. A full appreciation of secretory immune system is essential for understanding the pathophysiology of gastrointestinal diseases. However, many important problems remain still unsolved. There have been many conflicting reports about the cellular origin of secretory component(SC), which is one of the most basic subjects. Immunohistochemical studies of intestinal biopsies from patients with primary immunodeficiency diseases have been undertaken to obtain more information about the cellular origin of SC. We have found unique pictures in biopsy specimens from the duodenum of a patient with common variable immunodeficiency. A specific anti-SC antiserum did not stain the intestinal columnar cells, whereas goblet cells were shown to contain SC. The pathophysiological significance of such findings is discussed in relation to the literature.
    The cellular origin of SC, including discordant findings between human and animal intestine, should be continuously investigated.
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  • Serum GPT Values and Endocrine-Metabolic Features in Obese Subjects
    Michiyasu YOSHITSUGU, Toru IWASE, Ryushi SASSA
    1979 Volume 76 Issue 6 Pages 1277-1283
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    76 obese subjects, all of whom were 30 to 50 per cent above ideal body weight, were devided into the cases with normal and abnormal serum GPT values, and compared with the two groups about the fasting levels of serum insulin, blood glucose and serum lipids, and the degree of abnormality of the glucose tolerance tests (OGTT) in both sexes.
    1) In males 16 in 43 obese subjects had abnormal S-GPT values, and the fasting serum insulin and blood glucose levels of the group with abnormal S-GPT values were significantly higher than those of the group with normal S-GPT. There were no significant differences about the degree of the OGTT and the levels of serum lipids between the both groups with normal and abnormal S-GPT values.
    2) In females only 2 cases in 33 obese had abnormal S-GPT values.
    3) In both sexes the S-GPT values proved significantly related to the fasting serum insulin levels, that were lower in females than in males. The non-hyperinsulinism in the female obese may explain that there are few cases with abnormal S-GPT values.
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  • Yasutsugu KONO
    1979 Volume 76 Issue 6 Pages 1284-1294
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In an attempt to clarify the role of the liver in the histamine metabolism, the changes of the plasma histamine levels in the portal and hepatic venous blood, the number of the tissue mast cells in the liver and the in vitro histamine release in the liver were studied following the administration of either histamine chloride or compound 48/80 into intact and carbon tetrachloride treated dogs.
    The plasma histamine level of the portal blood was higher than that of the hepatic venous blood in both control and CCl4-treated dogs before the administration of the test drugs. The plasma histamine levels were similar in both control and CCl4-treated dogs after the administration of histamine chloride, while the level in CCl4-treated dogs was higher than that in controls after the administration of compound 48/80.
    There was no definite change in the number of the tissue mast cells in the liver of both control and experimental dogs.
    The rate of the release of histamine in the liver, tested in vitro, was much higher in CCl4-treated dogs than in controls.
    The important role of the liver in the histamine metabolism was indicated by the changes in the plasma histamine levels observed in the present study. Histamine is inactivated in the liver and the hepatic disturbance bring about the acceleration of histamine release.
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  • Akiharu WATANABE, Syosaku HAYASHI, Toshihiro HIGASHI, Takahiro OBATA, ...
    1979 Volume 76 Issue 6 Pages 1295-1305
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    An elemental diet of maltose and amino acid mixture containing mainly branched chain amino acids was applied to 5 patients with fulminant hepatitis and primary hepatoma, since they were complicated with catheter sepsis or intractable ascites. The continuation of the diet resulted in an improvement of negative nitrogen balance and normalization of distorted serum aminogram in these patients. The enteral alimentation is the most physiological nutritional supply, and the combination with intravenous hyperalimentation provides easily rational nutritional supporting system in patients with severe liver diseases.
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  • Satoshi NAKANO, Taku KUMADA, Kimio KITAMURA, Hajime WATAHIKI, Isao TAK ...
    1979 Volume 76 Issue 6 Pages 1306-1314
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Serum ferritin was measured and clinical evaluation was discussed on 198 patients with various diseases including hepatic malignancy.
    1) M.V. ± S.D. of serum ferritin in normal male and female persons were 58.9±44.8 and 27.3±24.0ng/ml respectively.
    2) In fourty three % of the patients with liver cirrhosis, 50% of hepatitis, choledochoand chole-lithiasis, 74% of hepatoma, 67% of metastatic carcinoma of liver, 38% of gastric cancer, 25% of colon cancer and pancreatic cancer and 88% of biliary malignancy, abnoemal values of serum ferritin were obtained.
    3) Significant correlation between serum ferritin and GOT was observed only in the patients with hepatitis. (P<0.01)
    4) In the patients with hepatoma, correlation between serum ferritin and AFP was significantly positive (P<0.05) if AFP was above 104 ng/ml, and negative (0.05<P<0.1) if AFP was below 104 ng/ml.
    Significantly high values of serum ferritin were obtained in the cases whose tumor was an large as above one third of the whole liver comparing with those, below 4×4cm in diameter. (P<0.05)
    In the two cases with hepatoma, serum ferritin persisted high values even though AFP dropped postoperatively.
    5) No significant correlation between serum ferritin and CEA was observed in the cases with primary and metastatic liver cancer but 78% of the patients having normal CEA and high serum ferritin was primary liver cancer.
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  • Chitose TOBARI, Masahiro NOGUCHI, Sachio OHTSUKA, Hiroshi KUROSAWA, Ma ...
    1979 Volume 76 Issue 6 Pages 1315-1324
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    New radionuclide technetium-99m-(Sn)-PI(Pyridoxylidene isoleucine)cholescintigraphy was performed for 31 patients with hepatobiliary diseases and 5 normal volunteer subjects.
    After intravenous injection, the serial scintigraphies of the normal subjects were obtained at about 5min. after for common bile duct, at about 12min. after for gallbladder and duodenum. These images were obviously comfirmed to be clear and early evaluation compared with prior I-131 compounds in clinically. In addition, the morphological and functional characteristics were satisfactorily evaluated for normal volunteer subjects, complete biliary obstruction, gallbladder stones, acute cholecystitis, biliary dyskinesia and hepatitis by the serial images.
    This radionuclide is combined with Tc-99m and physiological substances such as pyridoxal and isoleucine, so that this ideal radionuclide is strongly suggested to the clinical application instead of I-131 compounds because of low radiation doses and no side effects. Tc-99m-(Sn)-PI cholescintigraphy was also very available for the patients with hepato-biliary diseases from the new born to the aged for the diagnosis and follow up studies after operation and radiotherapy.
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  • Tetsuo TAKAYAMA
    1979 Volume 76 Issue 6 Pages 1325-1336
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Incomplete ligation of the main pancreatic duct resulted in formation of intraductal calculi in 16 out of 28 dogs after six months.
    The calculi were analized to be composed principally of calcium carbonate, which were similar to the human pancreatic calculi.
    Pancreatogram of dogs with pancreatic calculi showed marked dilatation of the pancreatic duct, but similar changes were also observed in dogs without pancreatic calculi six months after ligation.
    Histological alterations were periductal and perilobular fibrosis, and mild damage of parenchyma.
    Pancreatic secretory response to caerulein and secretin showed a marked decrease in flow, amylase and protein secretions, but concentrations of amylase and protein did not change significantly six months after ligation. Calcium and magnesium concentrations in the juce increased in the calcifying group.
    Endocrine function of the pancreas did not change significantly.
    From these results, it may be considered that the predisposing factors to calculus formation of pancreas are as follows; localized stasis of pancreatic flow, continnation of acinar secretion and qualitative changes of pancreative juice.
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  • Haruo MUGITANI, Sachio OHTUKA, Toshikazu SHINBO, Junichi YATA
    1979 Volume 76 Issue 6 Pages 1337-1349
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    IgG-FcR+T cells (subsets of the T cells) and PHA (phytohemagglutinin) response of lymphocytes and helper T cell activity were studied in 209 cases with cancer of the digestive organs. IgG-FcR+T cell showed 11.36±7.8% in the nontreated group and 7.98±6.3% in the group in which a tumor was removed.
    An increase in IgG-FcR+T cells and a decrease in PHA response were found in 5.8% of the patients with cancer stage I, in 42.8% of the patients with stage II, in 33.3% of the patients with stage III, and in 52.3% of the patients with stage IV. An increase in IgG-FcR+T cells and a decrease in helper T cell activity were found in 20% of the patients with stage I, in 20% of the patients with stage II, in 25% of the patients with stage III, and in 35.7% of the patients with stage IV. An increase in IgG-FcR+T cells and a decrease in PHA response were found in 12.5% of the patients with early stage of gastric cancer. On the other hand, an increase in IgG-FcR+•T cells and a decrease in helper T cell activitywe re found in 18.7% of them.
    An increase in IgG-FcR+T cells and a decrease in PHA response were found in 25.5% of the patients with advanced gastric cancer.
    An increase in IgG-FcR+T cells and a decrease in helper T cell activity were found in 37.9% of them.
    These results revealed an increase in suppressor T cells in patients with cancer of the digestive organs and a marked decrease in function of the T cells in according with the degree of cancer.
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  • Special reference to treatment of Zollinger-Ellison syndrome with cimetidine
    Keiko SHIRATORI, Sinichiro WATANABE, Yukiko OHTA, Masataka MARUYAMA, T ...
    1979 Volume 76 Issue 6 Pages 1350-1357
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hachinen AKITA, Hisashi TANIKAWA, Taketo KATSUKI
    1979 Volume 76 Issue 6 Pages 1358-1364
    Published: June 05, 1979
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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