Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 77, Issue 2
Displaying 1-12 of 12 articles from this issue
  • II. Growth Behavior of Various Histological Types and Prognosis of the Patient
    Katsunori NAKATANI, Yoichi KONISHI, Nobuyuki MIYAGI, Seiichi TAKAHASHI ...
    1980 Volume 77 Issue 2 Pages 173-178
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The growth behavior of various histological types of stomach carcinomas in nude mice and the prognosis of the patients were studied. Transplantability of 60 stomach cancer patients was 51% (31 out of 60 cases). The tumor growth pattern in the nude mice was classified as rapid type, slow type or persistent type. The growth pattern was not related to the histological types. However, there was a correlation between growth pattern and clinical stages of the patients. All of the tumors classified as stage 11 were the persistent type, and many of the tumors in stage III and stage IV were the rapid type. In Borrmann's classification, many of Borrmann 3 type tumors but none of the 4 type tumors showed the rapid type. The prognosis of the donor patients is as follows: In 8 cases whose tumors showed rapid type, 6 out of 8 died within 12 months after the operation and 2 out of them are still surviving 6 months after the operation; in 19 cases whose tumors showed persistent type, 7 out of 19 cases were survived more than 12 months and 9 out of them are still surviving.
    These results suggest that there might be a correlation between the growth pattern of the stomach carcinomas in nude mice and the prognosis of the patients.
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  • Junnosuke KOJIMA, Masako KANATANI, Nobuto NAKAMURA, Tohru KASHIWAGI, F ...
    1980 Volume 77 Issue 2 Pages 179-184
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Changes of glycylproline dipeptidyl aminopeptidase (GPDA) and r-glutamyl transpeptidase (γ-GTP) activities were compared in the serum and liver tissue of the rats with 3'-methyl DAB induced hepatoma. Subcellular distribution patterns of those enzymes were also studied in the process of carcinogenesis. Serum GPDA activity in rats with azodye-induced hepatoma was significantly higher than that in healthy rats, but serum GPDA activity was inclined to be elevated early after 3'-methyl DAB administration in comparison with the changes of γ-GTP. The specific activity of GPDA was decreased in the microsomal fraction and increased in the supernatant fraction of hepatic cancer tissue, suggesting that the enzyme is released from the microsomal fraction of the liver. Subcellular distribution pattern of GPDA was completely different from that of γ-GTP in hepatic cancer tissue.
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  • Tsuneo KIMURA
    1980 Volume 77 Issue 2 Pages 185-194
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The effect of bile acids such as chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA), cholic acid (CA) and deoxycholic acid (DCA) on the cultured cells (Chang's cells) was studied. Under the treatment with each bile acid, lactic dehydrogenase (LDH) activity in culture media and cell viability were measured, and morphological changes were studied with an electronmicroscopy. LDH activity was increased and cell viability was decreased by the addition of any of the bile acids. LDH activity in cell medium and cell viability were depending upon concentrations of these added bile acids. Under the treatment with CDCA (1.0mM), electronmicrograms revealed the cells treated with CDCA having wall-inclusion vesicles, dilated ER and edematous plasma membrane. Similar findings were seen in cells treated with UDCA (7.5mM), but they were attenuated as compared with CDCA treated cells.
    The degree of the cytotoxicity of bile acids was different each other. CDCA and DCA were more toxic, whereas UDCA and CA were less toxic. Taurine conjugative bile acids were less toxic to the cells than free bile acids.
    The increment in LDH activity by CDCA was decreased after preincubation with UDCA or by addition of UDCA and CDCA in the media. However, CA did not decrease the increment in LDH activity as UDCA did.
    These results suggest that the liver damage under administration of CDCA may be not only by the action of increased lithocholic acis but also by toxicity of CDCA itself.
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  • Takashi KANO, Hideko MIYAGAWA, Yasutoshi MUTO, Yoshiyata TAKAHASHI
    1980 Volume 77 Issue 2 Pages 195-205
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    It is well established that glucagon brings about elevation in plasma cyclic AMP (c-AMP) and stimulates glycolysis and gluconeogenesis in the liver which is a target organ of the hormone. The present study was undertaken to explore whether or not responses of both plasma c-AMP and glucose to intravenous injection of glucagon (1mg) become useful indicators to estimate hepatic reserve. In comparison with normal subjects, marked reductions in plasma c-AMP and glucose responses were observed in liver cirrhosis (8 cases) and none of the responses found in fulminant hepatitis (2 cases). On the other hand, patients with intrahepatic cholestasis (3 cases) revealed hyper-responders. In addition, significant correlations between the responses and liver function tests (i.e., hepaplastin test, choline esterase, ICG R15 and KICG) were observed in 6 normal subjects and 15 patients with various hepatobiliary disorders.
    These results strongly suggest that the responses of both plasma c-AMP and glucose to exogenous glucagon are of practical importance for evaluation of severity of hepatocellular damage as well as of clinical indication of glucagon-insulin therapy in fulminant hepatic failure. Further clinical and experimental data obtained in this study indicate that the reduction in response to exogenous glucagon may due to decrease of AT levels in the liver tissue and/or reduced binding capacity of glucagon receptor on the liver cell membrane under a continuous hyperglucagonemia in severe hepatic damage.
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  • Ken KIMURA, Kenichi IDO, Masahiko HORIGUCHI, Wakahiro NOGAMI, Yuzuru F ...
    1980 Volume 77 Issue 2 Pages 206-213
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    A method of percutaneous transhepatic portal catheterization (PTPC) is now widely applied not only for clinical diagnosis and treatment, but also for clinical investigations of the hepatoportal system. As clinical applications of PTPC, portography, measurement of portal pressure and even embolization of the gastric coronary vein in portal hypertension have become available. As clinical investigations, on the other hand, it is possible to perform comparative studies between portal and peripheral blood, for the purpose of investigating metabolic mechanism of the liver in a certain pathophysiological state. And, this technique of PTPC is now performed almost as a routine or an essential examination in the liver, biliary and pancreatic diseases in our department, obtaining useful informations which is decisive of diagnosis and treatment.
    PTPC was done in 78 cases (56 cases of hepatic diseases and 22 cases of biliary stones), with successful performance in 75 cases. The successful rate was high up to 96%. As to complications, one case of possible intra-abdominal hemorrhage, 2 cases of transient fever and 2 cases of temporal exacerbation of liver function tests were encountered in the present study, which were satisfactorily controlled by medical treatments. From the experience, PTPC is considered fairly safe procedure. The external diameters of the catheters were from 1.35 to 1.58mm.
    At the same time, technical method of PTPC, portal pressures in various hepatic disorders and serum levels of IRI and CPR in both portal and peripheral systems were reported.
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  • Akitaka NONOMURA, Joji HARATAKE, Tetsuji KARINO, Goroku OHTA
    1980 Volume 77 Issue 2 Pages 214-220
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    In our previous experiments, lymphocytes from patients with chronic active hepatitis (CAH) were highly cytotoxic to cultured rat liver cells (Coon cells) in comparison with lymphocytes from healthy persons. The present studies were undertaken to investigate a) the precise lymphooyte subset cytotoxic to Coon cells in patients with CAH and healthy persons and b) whether the Coon cell cytotoxic lymphocyte subset peculiar to CAH is present.
    Cytotoxic activity was found in the same lymphocyte fractions between patients with CAH and healthy persons. Cytotoxic activity was found in lymphocyte fraction of weak glass adherent cell, EA-RFC, EAC-RFC, low affinity E-RFC, aminoethylisothiuroniumbromide (AET)-treated E-RFC, non AET-treat E-RFC and non E-RFC, whereas lymphocyte fraction of non EA-RFC, non EAC-RFC and hot E-RFC did not show cytotoxicity. Among these cytotoxic lymphocyte fractions the weak glass adherent cell fraction from patients with CAH showed significantly higher % cytotoxicity in comparison with that of healthy persons. These results indicate that cytotoxic lymphocytes to Coon cells, both in patients with CAH and in healthy persons, are composed of heterogenous lymphocyte subsets, and Coon cell cytotoxic lymphocyte subsets peculiar to CAH are not present and highly cytotoxic activity of patients with CAH to Coon cells may be mediated by spontaneous cell mediated cytotoxicity (SCMC) or by other mechanisms mediated by lymphocytes indistinguishable from SCMC.
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  • ESPECIALLY ROLE OF THE CONJUGATED BILIRUBIN IN GALLBLADDER BILE
    Ikunoshin UEMATSU, Noriyoshi SUZUKI, Wataru TAKAHASHI
    1980 Volume 77 Issue 2 Pages 221-230
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Semiquantitative analysis of conjugated bilirubin (CB) in gallbladder bile was done by diazotization after Van Roy & Heirwegh's method and separation using thin-layer chromatography, in order to clear the role of conjugated bilirubin in bile of cholesterol gallstone cases, especially in mixed stone cases.
    Gallbladder bile was analysed in three groups, control (C) group, pure cholesterol stone (P-C) group, and mixed stone (M-S) group.
    Each proportion of azopigment in C-group was 13.7±1.2 (M±SE; %), 1.9±0.3, 4.9± 0.4, trace, 18.6±0.9, 60.9±1.9 in α0, α2, α3, β, γ, and δ fraction, respectively. In M-S group, there was a significant decrease (P<0.001) in proportion of δ fraction as compared with C-group. However, in P-C group, the proportion of azopigment showed approximately as same pattern as in C group. On the other hand, only in M-S group, there was significant negative correlation (P<0.01) between lithogenic index (L.I.) and α0/δ ratio (α0 is the most hydrophobic azopigment, and δ, the most hydrophilic one, therefore the increase of α0/δ ratio may show the increase of the hydrophobic tendency of CB in bile).
    It was deduced that, in the cases of lower L.I., stone formation needed the increase of the hydrophobic tendency of CB in bile, especially in mixed stone cases.
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  • Kyoko MIYASAKA, Hiroshi OKADA, Masataka HAYASHI, Yasushi KUYAMA, Yoshi ...
    1980 Volume 77 Issue 2 Pages 231-238
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Pancreatic involvement of 38 patients with Sjögren's syndrome was examined.
    Ten among 29 patients revealed abnormal findings in PS test and 5 among 13 in ERCP. These abnormalities were mild, however, not correlative to the stage of sialogram, and observed in the patients over the fifth decade in age.
    Beta 2 microglobulin concentration in pancreatic juice of the patients with Sjögren's syndrome was slightly elevated compared to that of control patients.
    The analysis of serum amylase isozyme demonstrated markedly low level of salivary type amylase in the patients with advanced changes in sialography, suggesting positive correlation between the concentration of serum salivary type amylase and the histological changes in the salivary glands.
    This study might suggest that the pancreas might be involved in the disease process of Sjögren's syndrome.
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  • Shinjiro YOSHIMOTO, Ryuji OHNISHI, Makoto BEPPU, Sachiko DOI, Michimas ...
    1980 Volume 77 Issue 2 Pages 239-245
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Endoscopic retrograde pancreatic parenchymography was performed in 152 cases, and good pancreatic parenchymogram was obtained in 70 cases, for whom, diagnostic possibility was pursued on chronic pancreatitis and pancreatic carcinoma by this method. In cases of severe chronic pancreatitis a partial or whole defects in pancreatic parenchymal picturers were showed by this method. While irregular pancreatic contour and non-uniform parenchymal picture were observed in moderate chronic pancreatitis.
    The pancreatic surface seen on X-ray in cm2 was divided by the greatest diameter of the main pancreatic duct in mm for obtaining the ratio of S/D, as a result, significantly lower value was obtained in the group of chronic pancreatitis as compared with normal group.
    By this method, pancreatic carcinoma was demonstrated as the filling defect of pancreatic field, and this method was useful for the diagnosis of carcinomatous invasion to the duodenal side of the pancreas and carcinoma which occurred at the peripheral part of the pancreas without affecting the main pancreatic duct.
    No particular and coincidental symptom was observed in 152 cases treated with this method.
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  • Makoto YAMAMOTO
    1980 Volume 77 Issue 2 Pages 246-255
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Salivary and pancreatic amylase clearance to creatinine clearance ratios in 19 patients with chronic renal failure, were compared with those obtained for normal control subjects.
    Hyperamylasemia was frequently observed. Both serum salivary and pancreatic amylase levels were elevated in most patients. The highest serum amylase activity was 774IU/L, less than 2 times value of normal upper limit.
    Amylase clearance (0.42±0.24 ml/min) was reduced markedly in chronic renal failure. Salivary and pancreatic amylase clearances changed in parallel to that of total amylase, but these clearances were by different ratios reduced to about one fifth (0.30±0.18ml/min, salivary) and one seventh (0.53±0.32 ml/min, pancreatic) of those in controls. Normal control values were 2.45±0.73ml/min (total), 1.45±0.68ml/min (salivary) and 3.69±1.01ml/min (pancreatic), respectively.
    Amylase clearance to creatinine clearance ratio of the patients with chronic renal failure (4.47±2.84%) was significantly higher (p<0.05) than that in controls (2.60±0.59%). The clearance ratio for salivary amylase (3.48±2.87%) was also significantly higher (p<0.01) than in controls. But this ratio for pancreatic amylase (5.33±2.81 %) was not significantly higher. Corresponding control values were 1.52±0.46% (salivary) and 3.96±0.97% (pancreatic).
    Of the nine patients who showed raised clearance ratios, eight had less than 10ml/min of creatinine clearance value and one had 10.2ml/min. Amylase clearance to creatinine clearance ratio in chronic renal failure was inversely related to creatinine clearance (r=-0.5587, p<0.05). All patients studied did not have clinical evidence of pancreatic disease, indicating that in the presence of severe chronic renal failure a high clearance ratio need not be indicative of acute pancreatitis.
    These results are compatible with the suggestion that in severe renal failure the loss of nephrons results in decreased fractional reabsorption of amylase in the tubules.
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  • Takeshi KASAJIMA, Haruhide SHINZAWA, Chikako TAKAHASHI, Shigeru HAGA, ...
    1980 Volume 77 Issue 2 Pages 257-261
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • M. HAYASHI, [in Japanese], [in Japanese], [in Japanese], [in Japanese] ...
    1980 Volume 77 Issue 2 Pages 262
    Published: February 05, 1980
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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