Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 77 , Issue 3
Showing 1-15 articles out of 15 articles from the selected issue
  • Kenji SAKURAI, Hiroyukis ITO
    1980 Volume 77 Issue 3 Pages 355-360
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In an attempt to do an objective analysis of the bowel sounds of the new borns which were recorded on magnetic cassette tapes, the frequency spectra and the distribution of the bowel sound durations were studied.
    For better sound/noise ratios, all recordings were made using our double cylinder suction type pick up apparatus.
    The spectrum analysis done by the soundspectrograph (SSG) and fast Fourier analysis (FFT) revealed that frequency did exceed 1500Hz only rarely and that the shaded stripe patterns drawn by the SSG corresponded well to the frequency spectra obtained by FFT.
    When the unit time is set to lmsec, the distribution probability of the sound duration follows Poisson's phenomenon, with relatively small variance.
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  • Yoshinosuke TANAKA, Kenzo KOBAYASHI, Masami MURAI, Teruyoshi KATAYAMA, ...
    1980 Volume 77 Issue 3 Pages 361-367
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We studied immunoadjuvant activity of phospholipids extracted from each homologous animal tissues (intestine, liver and brain) to elucidate the immunological role of the tissue phospholipids in autoimmune diseases including ulcerative colitis.
    Each tissue could elicit the delayed hypersensitivity and antibody production to protein antigen. Especially, negatively charged phospholipids fraction owned the strong activities as immunoadjuvants. Moreover, crude intestinal phospholipids had too the same activity to ECA, suggesting that intestinal phospholipids play some roles in the pathogenesis of ulcerative colitis.
    Slightly chronic inflammations of the colon tissue were observed in the ECA sensitized guinea pigs.
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  • Kazunori HOSHIKA, Toshihico KANOO, Nobumi HISAMOTO, Akira HUSHIMI, Jun ...
    1980 Volume 77 Issue 3 Pages 368-376
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    This report deals with some pathogenetic factors of Giardia induced malabsorption syndrome in patient with reduced secretory immunoglobulin A in duodenal aspirate. Control studies were carried out with asymptomatic giardiasis.
    In the patient with reduced SIgA in duodenal aspirate, Giardia trophozoites and bacteria are present in numberless on the duodenal mucosa and dense filamentous coating of trophozoite covered to fuzzy coat of the microvilli. It is possible that these conditions can cause some disturbance of the surface absorption process of microvilli.
    Competition by large number of Giardia and overgrowthed bacteria for dietary nutrients may be a factor of malabsorption.
    Flagyl effectively eliminated the parasites in asymptomatic patient, but the patient with reduced SIgA in duodenal aspirate, Flagyl-therapy was not effective as a result of oneyear-follow-up.
    In two healthy carriers of the cysts, after one year, giardia cysts can not detected in stool without treatment.
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  • Akitaka NONOMURA, Joji HARATAKE, Tetsuji KARINO, Goroku OHTA
    1980 Volume 77 Issue 3 Pages 377-385
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Normal lymphocyte cytotoxicity to cultured liver cells pretreated with test sera was studied according to the method of Kawanishi (Gastroenterology, 73: 549, 1977). Target liver cells used were two established cell lines; one was Coon cells established by Dr. Coon, H.G. in 1968 from adult rat liver and the other was RLC 1005 established by Dr. Tsunematsu, Y. in 1978 from faetal rat liver. And rat faetal fibroblast primary culture was used as control target cells.
    After preincubation of cultured liver cells with CALD (chronic active liver disease) sera, normal non-immune lymphocyte cytotoxicity against these cultured liver cells was significantly increased in comparison with control sera treatment. In an absence of the lymphocytes, sera tested alone did not showed cytotoxicity to the cultured liver cells. After treatment of rat fibroblasts with CALD sera, however, normal lymphocyte cytotoxicity to rat fibroblast was not increased in comparison with control sera treatment. Augmentation of normal lymphocyte cytotoxicity to CALD sera-treated liver cells was significantly blocked with aggregated IgG. And cytotoxicity was closely related to the presence of immunoglobulin capable of binding to target liver cell membranes in the CALD sera.
    These results indicated that cellular machanism of the augmented cytotoxicity reaction of normal lymphocytes against CALD sera-treated hepatocytes might an ADC C (antibodydependent cell-mediated cytotoxicity) and it might play some roles in pathogenesis of CALD.
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  • Masataka IWASAKI, Izumi MARUYAMA, Naoki IKEZIRI, Masahide ABE, Toyoaki ...
    1980 Volume 77 Issue 3 Pages 386-394
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Endotoxemia was frequently found in severe liver diseases; 75% of fulminant hepatitis, 61.9% of hepatoma and 56.8% of liver cirrhosis cases. Serum total bilirubin levels in fulminant hepatitis and liver cirrhosis showed to be lower in cases with endotoxemia in comparison with those of cases without endotoxemia. Endotoxemia was frequently found in liver cirrhosis and hepatoma cases in which liver scintigram of 99mTc-phytate showed marked atrophy.
    Endotoxemia was usually found in liver cirrhosis cases in which hepatic blood flow index by 99mTc-phytate was under 0.124 or BSP (45 min) and ICG tests were both over 34%.
    Disappearance of endotoxemia was noted in, about 50% of liver cirrhosis and hepatoma cases treated with Lactulose.
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  • IV. Electron Microscopic Studies on the In Vitro Interaction Between Human Lymphocytes and Human Cultured Hepatoma Cells
    Yoshio MUNAKATA, Masaya ODA, Toshio MORIZANE, Tetsu WATANABE, Kanji TS ...
    1980 Volume 77 Issue 3 Pages 395-409
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Scanning and transmission electron microscopic studies were performed on the cytotoxicity of the lymphocytes, from healthy human subjects or from patients with hepatoma, against human cultured hepatoma cells (LiC-1 Ohtsuka). The target cells were more strongly injured by the lymphocytes from healthy subjects, especially by non-E rosette forming cells with many microvilli, than by those from patients with hepatoma.
    Close contact between the lymphocytes and the target hepatoma cells was considered to be essential for target cell injury. At the initial stage of the mixed culture, the microvilli or the uropod projected from the lymphocyte surface were intimately connected with the microvilli on the target cell (interdigitation). After 24 hour incubation, more broad contact was seen between the plasma membranes of both cells (peripolesis), concomitant with many blebs on the target cell, indicating the degeneration of the cell. The plasma membrane of the lymphocyte deeply brought into the target cell like a pseudoinclusion body was entirely attached to that of the target cell (emperipolesis). In this form of cell contact, most prominant ultrastructural alterations, i.e. an increase in autophagic vacuoles and lysosomes and large bleb formation, were observed in the target cell. At the terminal stage, the target cell was found to be in a lytic necrosis. At high magnification of the cell contact area, the narrow gap (200-400 Å in width) was always present between the opposed plasma membranes. The intercellular gap, however, was filled with the ruthenium redpositive surface coats on the outer surfaces of both plasma membranes, which were closely associated or fused each other. The lymphocytes may affect the target hepatoma cell through the connection of both cell surface coats.
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  • Mamoru KOBAYASHI, Kazumi SATO, Hiroshi SHIMADA, Koichiro SHINMYO, Fumi ...
    1980 Volume 77 Issue 3 Pages 410-414
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Intravenous glucagon test was performed in twelve cases with obstructive jaundice and in eleven normal persons as control.
    Mean fasting blood sugar level was 103.mg/dl in the former and 94.1mg/dl in the latter. After glucagon injection, an average increment of the blood sugar level was 48.2mg/dl and 42.4mg/dl, respectively. The difference between two groups was not statistically significant. The average blood sugar level reached the fasting level before 60 minutes after glucagon injection in control group but not in jaundice group. This finding is interpreted to indicate accelerated glycogenolysis in obstructive jaundice.
    Two minutes after glucagon injection, mean serum insulin level reached 44.6μU/ml in jaundice group and 73.3μU/ml in control group, but it returned to the fasting level within 60 minutes in these two groups. There was no significant difference between two groups in the serum insulin level.
    As in our previous report, this immediate elevation of serum insulin was not noted in the cases with obstructive jaundice after intravenous glucose injection.
    Therefore, these findings seems to indicate the existence of the different mechanism of pancreatic β-cell stimulation between glucagon and glucose.
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  • Takashi SUZUKI
    1980 Volume 77 Issue 3 Pages 415-422
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The size changes of gallbladder were investigated in 99 subjects by ultrasonotomography after infusion of biligraphin and injection of caerulein. In normal controls, the enlargement ratio by biligraphin and the maximal contractile ratio by caerulein were 154% and 58.5% respectively. There was a significanct inverse correlation between the enlargement ratio and the maximal contractile ratio. The enlargement ratio was decreased in patients with liver cirrhosis and pancreas head cancer. The maximal contractile ratio was decreased in patients with cholelithiasis and pancreas head cancer. The results suggest that the measurement of enlargement ratio and of maximal contractile ratio is a useful tool for evaluating gallbladder function.
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  • Amylase Isoenzyme and Electron Microscopic Findings
    Toshio FUJIOKA
    1980 Volume 77 Issue 3 Pages 423-432
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Pancreozymin-Secretin test (PS test), endoscopic retrograde pancreatography (ERP) and quantitative measurement of serum amylase isoenzyme-P were performed in 101 patients suspected to have chronic pancreatitis. These data were compared with light and electron microscopic findings of biopsy specimens from 12 cases out of 101 patients.
    The mean value of amylase isoenzyme-P was 97 IU/l in normal subjects (n=25) and its value did not change before and after PS test. In the cases of minimal pancreatitis diagnosed by ERP (MIP), the value of amylase isoenzyme-P increased from 127 IU/l to 201 IU/l by the stimulation of pancreozymin and secretin (n=47).
    Light microscopic examination revealed only mild fibrosis and cell infiltration. Electron micrograph showed ruptured basement membrane, secretory granule fluxing into the stroma (SG), markedly dilated rough endoplasmic reticulum (RER) and swelling of mitochondria (M).
    Furthermore, filamentous materials were found in the lumen of acinal cells and intercalated portion and the obstruction of lumen by the plug of these materials played a role in the progression of the pancreatic damage.
    Measurement of serum amylase isoenzyme-P was thought to be useful for early diagnosis of the minimal pancreatic damage.
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  • Role of Acidic Mucopolysaccharides in Pancreatic Stone Formation
    Ryohei IZUMI
    1980 Volume 77 Issue 3 Pages 433-444
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to elucidate the mechanism of pancreatic stone formation, acidic mucopolysaccharides of human pancreatic juice and pancreatic stone were examined biochemically.
    And also, histochemical studies were made on the pancreatic ductal cells, in healthy and pathological conditions, which were estimated to secret the mucopolysaccharides into the pancreatic juice.
    The following results were obtained.
    Acidic mucopolysaccharides are composed of acidic mucoprotein, hyaluronic acid, heparan sulfate and chondroitin sulfate A. In chronic relapsing pancreatitis, acidic mucopolysaccharides of the pancreatic juice are composed of heparan sulfate mainly. But in chronic pancreatitis with severe fibrosis, acidic mucopolysaccharides of the pancreatic juice are composed of hyaluronic acid and acidic mucoprotein mainly. Composition of acidic mucopolysaccharides of pancreatic stone is similar to those of pancreatic juice in chronic relapsing pancreatitis.
    It is estimated that changes of the composition of the acidic mucopolysaccharides in pancreatic juice are related to the changes of the pancreatic ductal cells.
    From these biochemical and histochemical studies, the acidic mucopolysaccharides found in the pancreatic juice and stone were assumed to play important roles in the pancreatic stone formation.
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  • Minoru UKIDA, Sadaka IWAHARA, Yoshihiro SHIMADA, Yasuhiro ISHIKAWA
    1980 Volume 77 Issue 3 Pages 445-450
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Tadao KABAKINO, Norihumi MIYASAKI, Hakaru OTUKA, Hiroharu ISOMOTO, His ...
    1980 Volume 77 Issue 3 Pages 451-454
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Michio KIMURA, Yukihiro TSUCHIYA, Masao OHTO, Takanori ONO, Hiromitsu ...
    1980 Volume 77 Issue 3 Pages 455-460
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Kazuhiko OKABE, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1980 Volume 77 Issue 3 Pages 461
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Katsuhiro ENDO, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1980 Volume 77 Issue 3 Pages 462
    Published: March 05, 1980
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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