Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 78 , Issue 7
Showing 1-18 articles out of 18 articles from the selected issue
  • Akira NAKAYASU
    1981 Volume 78 Issue 7 Pages 1363-1369
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Effect of intravenous (i. v.) injection of Sulpiride on arginine-stimulated serum gastrin (GA) and growth hormone (GH) was studied in 5 normal subjects, 7 gastric ulcer patients (GU), and 8 duodenal ulcer patients (DU). Serum immunoreactive GA and GH were measured at preinjection and 1, 2, 5, 10, 15, 30, and 60 min, following i. v. L-arginine (0.1g/kg BW). On another occasion, 100 mg of Sulpiride with 200 ml of saline was infused for 45 min. and L-arginine (0.1g/kg BW) was injected 30 min. after the start of Sulpiride infusion. Serum immunoreactive GA and GH were assayed before Sulpiride infusion and at preinjection and 1, 2, 5, 10, 15, 30, 60 min. following i. v. Larginine.
    Serum immunoreactive GA response to L-arginine was significantly suppressed by Sulpiride infusion at 1 min. (p<0.05), 1 and 5 min. (p<0.05), and 1 min. (p<0.05) in normal subjects, GU, and DU, respectively. Serum immunoreactive GH response to L-arginine was significantly suppressed by Sulpiride infusion only in GU (at 30 min., p<0.05). Fasting immunoreactive GA and GH were not suppressed by Sulpiride infusion.
    It was demonstrated that 1) arginine-stimulated GA is significantly suppressed by Sulpiride infusion in normal subjects, GU, and DU, and 2) arginine-stimulated GH is significantly suppressed by Sulpiride infusion only in GU. It is suggested that 1) Sulpiride, dopaminergic receptor blocker, might suppress arginie-stimulated GA and GH through adrenergic receptor control mechanism, and 2) GH secretory mechanism in GU might be excessively sensitive to inhibitory effect of the adrenergic receptor control mechanism.
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  • Tomoaki URAKAWA, Kimiyuki HORI, Yuuichi MATSUNAGA, Shinzo NAITO, Yasuh ...
    1981 Volume 78 Issue 7 Pages 1370-1379
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The authors inflicted restrained and water-immersion stress on intact rats and investigated the correlation between stress ulcer formation and changes of amines and mucosubstances in the gastric mucosa, gastric motility, gastric blood flow and the pH of gastric juice were performed with the lapse of time in each rats. Also the effect of cimetidine and vagotomy on the prevention against an acute ulcer was discussed.
    The results were summarized as follows:
    1. The etiology of an acute gastric ulcer was at first due to the disturbance in the circulation in the gastric mucosa by the released amines and then due to the complicated factors such as gastric hypermotility, hyperacidity and lower gastric mucosal resistance.
    2. Cimetidine and vagotomy had a good preventional effect against stress ulcer formation.
    3. Cimetidine was assured to have a protecting effect against ulcer formation not only by the inhibitory effect of gastric secretion but also by the improvement effect of the circulation in the gastric mucosa.
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  • Hisashi MATSUMOTO, Osamu MASAMUNE, Yasunori NUNODE, Saburo OHSHIBA
    1981 Volume 78 Issue 7 Pages 1380-1387
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The purpose of this study is to compare oral bile acid tolerance test with vitamin B12 absorption test in order to evaluate the significance as the test to examine the absorptive capacity of ileum. Vitamin B12 absorption test has been used to determine the functional integrity of the ileum. As bile acid is also mainly absorbed from the ileum, it is presumed that change of serum bile acid after its oral administration reflects absorptive capacity of the ileum if liver function is not impaired. Upon this assumption, the change of the total serum bile acid was determined after 300 mg ursodeoxycholic acid oral administration. Bile acid was measured by enzyme-fluorometric method. Vitamin B12 absorption test was consecutively performed. Value was represented as ratio of urinary 57Co excretion to its oral dose. The subjects selected were five normal volunteers, 6 cases of gastroduodenal ulcer, 5 cases of ulcerative colitis and 4 cases of diffuse ileal disease. Following results were obtained; 1) The elevation of serum bile acid reached to the peak at 30 or 45 min after oral administration of 300 mg ursodeoxycholic acid in normal volunteers, cases of both gastro-duodenal ulcer and ulcerative colitis. While, there were no marked elevation of the serum bile acid in cases with diffuse ileal disease. 2) In vitamin B12 absorption test, urinary excretion of administered 57Co-V. B12 was less remarkable in cases with diffuse ileal disease than in cases with other gastrointestinal disease. 3) Correlation between bile acid tolerance test and vitamin B12 absorption test was demonstrated (r=0.64, Y=1.4+6.4, p<0.05).
    Therefore, it was concluded that bile acid tolerance test is available as the test for examining the absorptive capacity of the ileum.
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  • With Special Reference to Secretory Component Localization and IgATransport Mechanism
    Toshio ASAI, Hiroshi NAGURA, Keiichi WATANABE
    1981 Volume 78 Issue 7 Pages 1388-1398
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The peroxidase-labelled direct antibody method after Nakane was used for the localization of secretory component (SC) andIgAin colonic tumors and related disorders, e.g. adenocarcinoma, adenoma and hyperplastic polyp.SCwas found in identical ultrastructural sites in adenoma or hyperplastic polyp epithelium as in the epithelium from normal subjects, i.e. the perinuclear spaces, cisternae of the rough endoplasmic reticulum, lamellae and vesicles of the Golgi complexes, basolateral plasma membrane (but not apically beyond the tight junctions) and vesicles in the cytoplasm. The immunohistochemical characteristics ofSC, i.e. amount and localization in adenomas and hyperplastic polyps, are closely aligned with those of the normal colonic mucosal epithelia showing maturity of varying degrees. That is, the epithelia of adenomas exhibit immaturity, whereas those of hyperplastic polyps indicate hypermaturity. For instance, the immunoelectron microSCopic observation revealed that the immature goblet cells in adenomas exhibited SC synthesis, while mature goblet cells contained littleSCin their cytoplasm. These results suggest that SC could be a good indicator for maturation and differentiation of colonic epithelial cells. In contrast to those benign tumors, the staining intensity and intra-and extra-cellular fine localization of SC were quite variable from cell to cell in adenocarcinoma. In the adenocarcinomasSCwas demonstrated not only on apical plasma membranes and the surfaces of microvilli of the neoplastic cells, but also in the epithelial interstices and surrounding connective tissues. These immunohistochemical observations provided a morphological evidence of "loss of polarity" in malignant cells. Although theSC-mediatedtransportmechanism for dimericIgAwas rather well maintained in these colonic tumors, tranSCellulartransportfor sIgAthrough the neoplastic cells was on occasion retarding.
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  • Mikio ZENIYA, Yumiko KATO, Hiroshi TAKAHASHI, Masamichi DEURA, Yoshika ...
    1981 Volume 78 Issue 7 Pages 1399-1404
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    To clarify the immune mechanism of cytotoxicity in chronic active hepatitis, the liver cell injury by antibody dependent cell-mediated cytotoxicity (ADCC) was studied, lymphocyte cytotoxity was observed by micro-cytotoxicity assay in patients with chronic active hepatitis using isolated rat hepatocyte coated by patients serum as a traget cell and normal peripheral blood lymphocytes as a effector cell, with a ratio of target cell to effector cell of 1: 50. More prominant cytotoxicity was observed in T-depleted lymphocyte fraction.
    After absorption of the patients' serum with LSP (Meyer zum Buschenfelde) lymphocyte cytotoxicity was decreased. These result show in chronic active hepatitis ADCC may play a role of liver cell injury and LSP was one of the important factor to mediate the cytotoxicity. But the percent population of K-cell in peripheral blood was decreased in chronic active hepatitis. This change of Kcell population show that the inhibitory factor of K-cell exists beside the antibodies which induce ADCC in Patients serum.
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  • Yasuhiro MIZOGUCHI, Hiroko TSUTSUI, Yoshihide SAKAGAMI, Takashi SHIBA, ...
    1981 Volume 78 Issue 7 Pages 1405-1411
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The specific antibody against the liver cell membrane was coated to the isolated liver cells and liver cell damage was induced by adding the peripheral blood lymphocytes as the K cell source. When liver injury was estimated by the determination of albumin biosynthesis in liver cells, a marked reduction of the synthesis was observed by ADCC reaction.It was also shown that intracellular levels of lipid peroxide were significantly increased by the ADCC-mediated liver cell damages.Moreover, a cytotoxic factor which caused the liver cell injury and the increase of intracellular lipid peroxide formation was detected in cell culture supernatant of ADCC reaction.This factor was fractionated by the gel filtration using a Sephadex G-75 column.Both factor acting cytotoxic on the isolated liver cells and factor causing the lipid peroxide formation in liver cells were detected in the same fraction of culture fluid.
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  • Takero NAKAGOMI
    1981 Volume 78 Issue 7 Pages 1412-1418
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to study the intrahepatic cholestasis by the experimental alcoholic liver injury, effect of ethanol on bile canalicular structure and it's function were studied. Male-Donryu rats were fed on the 36% ethanol-containing liquid diet for 1 and 9 months.
    After 9 months feeding, the bile canaliculi were dilated and microvilli were lost and decreased in number, these changes were already observed after 1 month, though not so remarkable.
    On 1 month feeding, bile flow was significantly decreased and bile acid secretion was also decreased, and serum bile acid concentration elevated by two times. As to biliary bile acid composition, deoxycholic acid decreased by a half and ursodeoxycholic acid increased by about four times.
    These ultrastructual and functional changes were quite similar to the drug-induced intrahepatic cholestasis.
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  • Kazuo FUKUDA
    1981 Volume 78 Issue 7 Pages 1419-1430
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In 50 subjects with liver cirrhosis lower esophageal sphincter (L.E.S.) function was evaluated by measuring basal LES pressure (LESP) and LES response to the administration of intravenous tetragastrin or oral glycine, concerning to evaluate the presence of gastroesophageal reflux. Basal LESP in liver cirrhosis was not significantly different from that of 16 healthy controls. There was no correlation between basal LESP and serum concentration of endogenous gastrin, estrone or progesterone. However basal LESP significantly increased up to 244±45 mmH2O in patients with histroy of variceal bleeding. It was suggested that anatomical factors such as variceal form, protal hypertension and/or ascites were attributed to this rise of basal LESP. No response of LESP to exogenous gastrin administration was recognized in 9 of 36 cases with liver cirrhosis. Also the decrease of LESP response to oral glycine administration was significant in liver cirrhosis.
    These data indicated that the gastroesophageal reflux would easily appear more frequently in patients with liver cirrhosis than healthy controls. Therefore the accurate evaluation of LES function should be done by studying the changes of LESP in various stimulation tests.
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  • Atsushi KIYOHASHI, Hisao SHIBATA, Sadanobu OZAWA, Motonobu ICHINO, Sho ...
    1981 Volume 78 Issue 7 Pages 1431-1436
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Serum 5'-NPD-V was assayed in the patients with hepatocellular carcinoma, metastatic carcinoma of the liver and benign liver diseases. All cases with jaundice (serum bilirubin_??_2.5 mg/dl) were excluded from the analysis, because jaundice can cause a non-specific positive reaction in this assay. Eighteen normal individuals were all negative for 5'-NPD-V. 12 out of 13 patients with hepatocellular carcinoma were positive. Negative results were found in 67% of 60 patients with liver cirrhosis. 75% of 36 patients with metastatic carcinoma of the liver were positive, only 15% of 34 patients in whom liver metastasis was not detected by surgery, angiography, CT scan or liver scintigram were positive.
    From the fact that 5'-NPD-V was detected even in 4 hepatocellular carcinoma patients with normal levels of a-Fetoprotein and the positive rate of 5'-NPD-V was higher than that of CEA in patients with metastatic carcinoma of the liver, 5'-NPD-V is considered a potential biochemical means for diagnosing hepatocellular carcinoma and metastatic carcinoma of the liver in combination with other tumor markers.
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  • Junichi TAZAWA, Namiki IZUMI, Yasushi HASUMURA, Jugoro TAKEUCHI, Masaa ...
    1981 Volume 78 Issue 7 Pages 1437-1442
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    To see whether Non-A, Non-B virus infection would involve the etiologic significance in non-B, non-lupoid (cryptogenic) type of chronic active hepatitis (CAH), 32 patients, diagnosed histologically, were divided into three groups (9 cases of hepatitis B virus related type, 10 cases of lupoid type and 13 cases of non-B, non-lupoid type), and clinico-pathological features of the non-B, non-lupoid type were compared with those of other two groups.
    Clinical features of non-B, non-lupoid type of CAH were showed to be apparantly different from those of lupoid type of CAH: Instead, it was found that clinico-pathological features of non-B, non-lupoid type were comparatively similar to those of hepatitis B virus related type. In non-B, nonlupoid type of CAH, however, a history of blood transfusion was common. it is likely, therefore, that Non-A, Non-B virus infection plays a role in inducing non-B, non-lupoid type of CAH.
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  • Masaaki EBARA, Yukihiro TSUCHIYA, Masao OHTO
    1981 Volume 78 Issue 7 Pages 1443-1451
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We have deviced new techniques for percutaneous transhepatic bile drainage (PTBD), one under fluoroscopic control using a long guide-stylet and the other with realtime ultrasonic guidance using a puncture transducer. The fluoroscopic method was successful in all of the 187 patients who uderwent the procedure, and the real-time ultrasound method was successful in 66 of 68 patients. There were no serious complications caused by these procedures except for one patient who developed a massive intraperitoneal hemorrhage due to inadvertent puncture of hemangioma of the liver. The performance of ultrasonic procedure was particularly easy and reliable because both the puncture needle and the aimed bile duct were observed during the procedure, making prior PTC unnecessary. It was safely performed even in patients suffering from severe acute suppurative cholangitis, and no serious complications due to the performance occurred.
    Decompression of the biliary tract by these procedure was effective in decreasing jaundice and suppressing infection in patients with mechanical obstructive jaundice and/or acute suppurative cholangitis.
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  • Seiki MATSUNO, Eishi MIYASHITA, Jun MATSUMOTO, Koichi SASAKI
    1981 Volume 78 Issue 7 Pages 1452-1458
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Determinations were made of the Zn and Mg contents of pancreatic juice in 12 cases undergoing external pancreatic duct drainage following pancreaticoduodenectomy in a study undertaken to investigate the output and excretion pattern of trace elements in pancreatic juice and also to explore their relations to pancreatic enzyme secretion and fibrosis of the pancreas. The results indicate that the output of Zn in pancreatic juice ran parallel with amylase activity and was found to be closely related to secretion of pancreatic enzymes. Relatively smaller variations were noted of the Mg content in pancreatic juice during the postoperative course, with the pattern of Mg excretion in pancreatic juice being dependent upon the amount of pancreatic juice secreted. The output of both Zn and Mg in pancreatic juice was found to correlate with the volume of the residual pancreatic parenchyma. The amylase/Zn concentration ratio for pancreatic Juice remained stationary postoperatively in all cases and was shown to have a negative correlation with the parenchymal volume. The findings suggest that the amylase/Zn concentration ratio can be a reliable indication of the degree of pancreatic fibrosis or of pancreatic exocrine function.
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  • Hideki NISHIWAKI, Katsusuke SATAKE, Kaoru UMEYAMA, W.Y. Chey
    1981 Volume 78 Issue 7 Pages 1459-1465
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The effect of pancreatic juice diversion from the duodenum on plasma secretin level and pancreatic secretion after meal ingestion were investigated in 5 dogs with pancreatic fistula and gastric canula.
    Exclusion of pancreatic juice resulted in a mean peak secretin level of 80.6±18.2 pg/ml and 8.3±1.7 ng/ml/150 min. of integrated scretin response for 150 minutes after meal ingestion; this coincided with a significant increase in pancreatic volume and bicarbonate out put. However, reinfusion of the collected pancreatic juice decreased the response of secretin secretion; a mean peak level of 57.0±15.0 pg/ml and the integrated secretin secretion of 5.0±0.8 ng/ml/150 min, which was acompanied by a decrease in pancreatic volume and bicarbonate output.
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  • Isao TAKEDA, Satoshi NAKANO, Kimio KITAMURA, Hajime WATAHIKI, Hiroshi ...
    1981 Volume 78 Issue 7 Pages 1466-1469
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yuji TANAKA, Hatsumi HASHIBA, Kazunori KUMADA, Yasuhiro SUGA, Yoshifuj ...
    1981 Volume 78 Issue 7 Pages 1470-1474
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hideo AMANO, Kazuo HARIMA, Tsuyoshi AIBE, Yuji NACATOMI, Masao KAWASHI ...
    1981 Volume 78 Issue 7 Pages 1475-1479
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • N SATO, [in Japanese], [in Japanese], [in Japanese], [in Japanese], [i ...
    1981 Volume 78 Issue 7 Pages 1480
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hideyasu KIYOHARA, [in Japanese]
    1981 Volume 78 Issue 7 Pages 1481
    Published: July 05, 1981
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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