Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 79 , Issue 3
Showing 1-16 articles out of 16 articles from the selected issue
  • Kazuhide MORINO
    1982 Volume 79 Issue 3 Pages 765-771
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Fourteen male 6 months old beagle dogs were given solution of N-ethyl-N'-nitro-N-nitro-soguanidine at a concentration of 150μg/ml or 100μg/ml for 5 to 9 months.
    Eight out of 14 dogs developed esophageal squamous cell carcinoma, and four developed epithelial dyslasia of the esophagus without squamous cell carcinoma. Good correlation was found between the conditions of administration of ENNG and the induced esophageal lesions. For the induction of squamous cell carcinoma, administration in the condition of 150μg/ml for 6 months or 100μg/ml for 9 months was suitable. For the selective induction of early esophageal carcinoma, the condition of 100μg/ml for 8 months was suitable.
    For the induction of epithelial dysplasia without carcinoma, 100μg/ml was suitable. It was clarified that by the selection of the concentration and the period of administration of ENNG, it is possible to induce esophageal squamous cell carcinoma or epithelial dysplasia selectively.
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  • Hiroshi SAKAUE
    1982 Volume 79 Issue 3 Pages 772-781
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to investigate the influence of acute liver injury on gastric secretion, gastric analysis was performed on eighteen(18) patients with acute hepatitis in its icteric and recovery stage. The maximal acid output, peak acid output and peak acidity were found to be significantly more elevated in the icteric stage than in the recovery stage, suggesting a status of increased acid secretion in the former. To clarify the cause, the influence of serum gastrin, plasma histamine and the vagus nerve on gastric secretion was studied. The results suggest that the gastric hypersecretion in the icteric stage was mainly due to increased vagal tone with minimal contribution on the part of serum gastrin and plasma histamine.
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  • Itsuo AMIOKA, Terukatsu ARIMA, Shin OKAMOTO, Hideo NAGASHIMA
    1982 Volume 79 Issue 3 Pages 782-789
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The relationship between gastric mucosal prostaglandin (PG) and acute gastric mucosal lesions caused by oral administration of phenylbutazone 200mg/kg was examined in rats. PGE and PG (A+B) were extracted, separated from the PG group on silicic acid column chromatography, and converted into PGB by alkaline treatment. Following above procedures, the PG was measured with the RIA double antibody method using anti-PGB1 serum.
    In the control rats, PGE was found approximately 10 times higher in concentration compared to PG (A+B). PG quantities in the experimental rats given the phenylbutazone were, both PGE and PG (A+B), significantly lower (p<0.001) at 6 hours after administration. And the PG remained low at 54 hours after, by which time the lesions had healed. These data shows that recovery from gastric mucosal lesions and the increase of endogenous PG are not related to each other.
    Furthermore, proglumide, an agent that promotes healing of ulcers, was administrated to determine whether or not the increase of gastric mucosal PG facilitates the recovery from gastric ulcer lesions. The accompanying increase of endogenous gastric mucosal PG was not observed. Therefore, proglumide did not cause the increase of gastric mucosal PG.
    These results proved that the decrease of endogenous gastric mucosal PG is related directly to the cause of acute gastric mucosal lesions. But, recovery from acute gastric mucosal lesions is not necessarily accompanied by the PG increase.
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  • Hiroshi MIKI
    1982 Volume 79 Issue 3 Pages 790-797
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The method for microassay of tissue plasminogen activator in gastric mucosa specimen obtained by endoscopy was studied by modifying the Astrup's method, and established a new useful and highly reproducible micromethod, applying 0.02% fibrin-agar plate for the preparative extract of tissue plasminogen activator that the activity had a linear relationship in semilogarithmic plotting with the diameter of lysis windows.
    Using this method, the change in plasminogen activator activity in the gastric mucosa was investigated in patients with gastric ulcer.The mucosal specimens were obtained at the margin of ulcer and at the distal area from ulcer by endoscopy, and control specimens were taken from mucosa of normal stomach.
    The plasminogen activator activity in the gastric mucosa of patients with active gastric ulcer showed a significant increase regardless of distance from ulcer. Further detailed observations, however, revealed that the increased activity was decreased significantly after medical treatment.
    On the other hand, the activity in the gastric mucosa of patients with ulcerated gastric cancer showed normal.
    These results suggest that the increase of tissue fibrinolytic activity in the gastric mucosa is involved in the formation of active gastric ulcer.
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  • Saku Tomita
    1982 Volume 79 Issue 3 Pages 798-807
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    VIP release in response to various factors was studied in normal subjects and patients with peptic ulcer. The mechansim of VIP release was also investigated by in vitro experiments using a perifusion system. Plasma VIP was measured by radioimmunoassay after extraction with acidacetone method. The fasting levels of plasma VIP were 86±4 pg/ml and no difference was observed on sex and age in control subjects. The plasma VIP levels were within normal range in the majority of patients with peptic ulcer.
    The ingestion of a meat soup, protein, sodium-taurocholate or ursodeoxycholic acid elevated plasma VIP levels as well as duodenal acidification. However, oral administrations of glucose and fat, and intravenous infusions of calcium and pancreatic hormones did not show any effect on plasma VIP levels.
    Oral administration of ursodeoxycholic acid had no effect on plasma VIP levels in three out of four patients with duodenal ulcer. The in vitro experiment demonstrated that VIP release from human duodenal mucosa into the perifusate was markedly stimulated by the perifusion of solution of pH 2 and taurocholate (1 mM and 5 mM), but not affected by those of glucose, fatty acid, amino acid, insulin and glucagon. Somatostatin showed no effect on basal level of VIP from the duodenal mucosa.
    These results indicated that VIP release induced by meal ingestion depends upon the combination of food components and that duodenal acidification and bile salts may participate, at least in part, in the postprandial VIP release.
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  • Yoshio MUNAKATA
    1982 Volume 79 Issue 3 Pages 808-819
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Three types of the lymphocytic cytotoxicities in vitro (NK: natural killing, ADCC: antibody dependent cell-mediated cytotoxicity, CMC: cytotoxic T lymphocyte (CTL) -mediated cytotoxicity) against Chang liver cells and the human hepatoma cell line (HCC-M) were studied by electron microscopy. Healthy human non-E rosette forming lymphocytes were added to Chang liver cells and HCC-M in the NK assay. They were also added to Chang liver cells pretreated with a rabbit anti-LSP serum and to HCC-M pretreated with a rabbit anti-HCC-M serum in the ADCC assay. In the assay of CMC, the induction of CTL was performed by the mixed lymphocyte tumor cell culture (MLTC), which was the 4-day mixed culture of HCC-M treated with mitomycin C and the healthy human E-rosette forming cells. Close contact between the lymphocytes and the target cells was essential for any type of the cytotoxicities. Especially, the ruthenium red-positive surface coats on the outer surfaces of both cell plasma membranes were thickened in part and closely associated with each other. This connection of both cell surface coats was considered to be most important for the lymphocytic cytotoxicities. By scanning electron microscopy, NK cells were shown to be villous on cell surface, while K cells relatively villous. Compared with NK and K cells, CTL appeared to be smooth on the surface. NK cells were about 5.5-6.0μ in diameter, whereas K cells were smaller (4.4-4.8μ) and CTL were larger (6.0-6.3μ) . The point contact forms (PC) were frequently seen at the early stage of the mixed culture, whereas the broad contact forms (BC) were observed in the later stage. PC was more abundant in NK and ADCC, while BC was more numerous in CMC. The number of target cells showing emperipolesis observed by light microscopy was large at the later stage of the mixed culture than at the early stage. Emperipolesis was more frequently seen in NK and ADCC, as compared with that in CMC. Especially, this was most abundantly observed in ADCC against Chang liver cells. Therefore, emperipolesis was considered to be the most important cytotoxic contact form in ADCC.
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  • Michiyasu YOSHITSUGU, Misao MAEHARA, Hitoshi SUGAYA, Takashi HARADA
    1982 Volume 79 Issue 3 Pages 820-826
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    A relationship between hepatic lipid content and various blood hormone levels including insulin (IRI) was studied in 23 obese subjects showing more than 15% over ideal body weight without diabetes mellitus and alcohol abuse (14 men, 9 women).
    The hepatic lipid content in obese patients did not correlate with fasting blood glucose levels and the sum of glucose values during 50g OGTT (∑BS), but positively with basal levels of serum IRI, the sum of IRI values during OGTT (∑IRI), ΔMRI/ΔBS at 30 min, ∑IRI/∑BS and fasting FFA levels. Thus it is suggested that hyperinsulinemia due to a diminished insulin sensitivity of the peripheral tissues is of importance for hepatic fatty change in the obese subjects.
    The hepatic lipid content was not influenced by basal levels of cortisol, thyroxine and growth hormone.
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  • Naoki SAOTOME, Eii KARASAWA, Makoto MIKI, Takatsugu UENO, Masao OHTO, ...
    1982 Volume 79 Issue 3 Pages 827-835
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Linear array electronic real-time ultrasonography proved to be useful for the diagnosis of obstructive changes of the bile ducts and differential diagnosis of jaundice.
    Forty control subjects and 83 patients with biliary tract diseases constituted the groups not associated with jaundice, and 142 patients with obstructive jaundice and 33 patients with hepatic diseases made up the jaundiced groups.
    The bile ducts were visualized in all of the control subjects and the diameters less than 5.5mm. In the patients with hepatic diseases, diameters of the bile ducts were the same as those in the control. In the patients with obstructive changes of the bile ducts, they were significantly dilated. In patients with bile duct stones but not jaundiced, they were dilated from 5 to 15 mm.
    In patients with jaundice due to obstructive diseases of the bile ducts, sites and ends of the obstructing lesions were demonstrated with a high success rate, and most of the lesions were correctly diagnosed.
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  • Yoshihiro TAKASAWA, Noriyoshi SUZUKI, Wataru TAKAHASHI, Ikunoshin UEMA ...
    1982 Volume 79 Issue 3 Pages 836-844
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The authors examined on dissolution and disintegration of calcium bilirubinate stone in human bile on the basis of the fundamental studies performed at our department.
    The results obtained were as follows; 1) Slice of calcium bilirubinate stone was readily soluble in the bile with a single addition of tetrasodium salt of ethylenediaminetetraacetic acid (EDTA•4Na). 2) Calcium bilirubinate stone was dissolved in bile with addition of all the three agents of EDTA•4Na, ursodeoxycholate (URSO), and heparin in a more accelerated manner than or in a comparable rate to the case of bile with single addition of EDTA•4Na. 3) The pH of the bile with addition of the triple combination of EDTA•4Na-URSO-heparin was 8.72±0.15, which was slightly more neutral than the case of bile with a single addition of EDTA•4Na (pH9.55±0.05), 4) In the dogs with bile duct dilatation, no abnormality was seen in the results of the hepatic function tests and serum calcium level during the period of irrigation of bile dut for 4 weeks. Nor was there any abnormality in gallbladder and the common bile duct histopathologically. No finding of toxic hepatitis was seen in liver.
    In conclusion, the solution of the triple combination of EDTA•4Na-URSO-heparin was found to be effective for dissolution and disintegration of calcium bilirubinate stones, and it was estimated that the solution would have extremely little disturbances to human bodies.
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  • Atsushi OHKI, Makoto OTSUKI, Choitsu SAKAMOTO, Hosai YUU, Shigeaki BAB ...
    1982 Volume 79 Issue 3 Pages 845-849
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The effect of endogenous secretin on pancreatic growth and exocrine function was investigated. To release endogenous secretin, 500mg/Kg B.W.1-phenyl-1-hydroxy-n-pentane(PHP) was give via orogastric tube once a day for 20 days or twice a day for 10 days.
    The pancreatic wet weights and pancreatic amylase contents in rats treated with oral PHP 500mg/Kg B.W. twice a day for 10 days and once a day for 20 days were not different from those in controls treated with 0.15M NaCl solution. Time course of amylase output and juice flow in respose to 0.1ng/ml caerulein from the isolated pancreas treated with oral PHP was the same as that from normal controls. Moreover, basal, maximal and the total outputs of amylase and pancreatic juice were not influenced by oral PHP treatment.
    Although oral PHP stimulates endogenous secretin release, oral administration of PHP once a day for 20 days and twice a day for 10 days had no effect on pancreatic exocrine function.
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  • Hisao OGUCHI, Atsuo NAGATA, Hidemitsu HIRABAYASHI, Kentaro TAKEUCHI, Y ...
    1982 Volume 79 Issue 3 Pages 851-857
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In the follow-up study of 53 patients with chronic calcifying pancreatitis, 5 patients developed extrapancreatic malignancy (9.4%). On the other hand, we found no pancreatic cancer in this series. There were, however, 4 patients with pancreatic cancer coexisting pancreatic calcifications, 3 cases of whom showed large stones with characteristic cast-like appearance.
    The incidence of malignancy in 211 autopsied cases with pancreatic lithiasis was investigated in annual reposts by Japanese Society of Pathology for recent 10 years between 1969 and 1978. Of these 211 cases, 31 had a pancreatic cancer (14.8%) and 55 had an extrapancreatic malignancy (26.2%).
    These results indicate that complications of pancreatic and extrapancreatic malignancy should be kept in mind during the follow-up of patients with pancreatic lithiasis.
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  • Ribun MIZUNO, Tetsuo HAYAKAWA, Aiji NODA, Takaharu KONDO, Akira SAKAKI ...
    1982 Volume 79 Issue 3 Pages 858-863
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    A method for duodenal chymotrypsin determination was deviced using glutaryl-L-pheny-lalanine-p-nitroanilide(GPNA) as a substrate and diazo-reaction for colorimetry.
    This GPNA method was simple, accurate and specific for duodenal chymotrypsin determination. Chymotrypsin activity obtained by the GPNA method correlated significantly with that by a benzoyl-L-tyrosine ethylester(BTEE) method.
    Duodenal chymotrypsin was significantly inactivated at 37°C Cafter 15 minutes or at 20°C after 60 minutes, but it was stable at 0°C for 2 hours or at -20°C for 7 days.
    Duodenal chymotrypsin output during pancreozymin secretin test had significant correlation with both amylase and trypsin outputs. In pancreatic cancer, chymotrypsin output decreased more than the amylase output. This dissociated reduction of chymotrypsin suggests that the chymotrypsin output can be a sensitive parameter for the exocrine pancreatic insufficiency caused by pancreatic cancer.
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  • Shigeru TSUKAGOSHI, Toshihiko SHIMODA, Tsutomu KARASAWA, Toshio SHIKAT ...
    1982 Volume 79 Issue 3 Pages 864-871
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Hepatitis B virus (HBV) has been considered to be replicated solely in human or primate liver cells, thus, an organotropic virus. However, recent studies indicated the possibility of extrahepatic replication of HBV including in the pancreas. Along the line of the proposed possibility, the present study is intended to detect HBs Ag and HBc Ag in the pancreas by immunohis-tological study.
    Thirty eight patients with HBs antigenemia were studied by indirect and direct immuno-fluorescent methods, and indirect and direct immunoperoxidase methods, using formalin-fixed, paraffin-embedded sections of pancreatic tissue, to detect HBs Ag and HBc Ag. Shikata's orcein technique was also done for HBs Ag detection. Specificity was checked by absorption test for indirect methods, and blocking test for direct methods.
    HBs Ag was positively stained in 21 patients out of the 38 patients, and the localization of HBs Ag was proved in pancreatic acinar cells, intralobular and interlobular ductal cells. HBc Ag was detected only in pancreatic acinar cells in the 6 cases which also had HBs Ag in the pancreas. HBV associated antigens were not found which Langerhans' islets. Control studies using 25 patients with no HBs antigenemia were all negative.
    This study proved the localization of HBs and HBc Ag in pancreatic acinar cells and ductalcells. The preasent study suggests further evidence of HBV replication in the pancreas.
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  • Koji YAKABI, [in Japanese], [in Japanese], [in Japanese], [in Japanese ...
    1982 Volume 79 Issue 3 Pages 872-875
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Tomikazu YAMAMOTO, Kazuichi OKAZAKI, Fuminori MORIYASU, Tadahiko SHIOM ...
    1982 Volume 79 Issue 3 Pages 876-879
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Keiko YAMAUCHI, Kazuyuki YOSHIZAKI, Tatsuo NANBA, Kazuya HIGASHINO
    1982 Volume 79 Issue 3 Pages 880-884
    Published: March 05, 1982
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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