1) An isotonic saline solution containing 154mEq/l sodium, and 154mEq/l chloride was instilled into the pylorus- and cardia-ligated stomachs of intact and common-bile-duct-ligated rats for 1-hour period. 8ml of the test solution were instilled into the stomach, and immediately after the instillation, 3ml of the fluid were aspirated from the stomach (sample 1). After lapse of 1-hour test period, the fluid was gently aspirated until the stomach was collapsed (sample 2). Sample 1 and sample 2 were served for quantitative analysis of hydrogen, sodium, potassium, and chloride ions concentration. Under restraint, the test for 1-hour period was serially repeated 5 times in the each rat. Cimetidine 25mg/kg was intraperitoneally administered in 3 groups (intact and icteric) of rats, in which the same instillation experiment as above was done.
2) Jaundice had no influence on net gain and loss of electrolytes in saline solution instilled into the stomach of rats.
3) The rate of suppressing gastric acid output with administration of Cimetidine 25mg/kg was between 39.9% and 65.3%.
4) Irrespective of presence or absence of jaundice and of administration or none of cimetidine, it was found in all but one test periods that the sum of net gain of cations (H
+, Na
+, K
+) was almost equivalent to net gain of anion (Cl
-).
5) On the basis of Hollander's two component hypothesis, an autoregulation-system, which kept the net ion output"cations minus anion"in the parietal component at an almost equivalent level to the net ion output"anions minus cations"in the nonparietal component, was supposed to be acting in the rat stomach under restraint.
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