Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 82 , Issue 8
Showing 1-22 articles out of 22 articles from the selected issue
  • Yoshitake IKEDA
    1985 Volume 82 Issue 8 Pages 1843-1852
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The mechanism of cysteamine induced ulcer formation has long been known to be due to increased acid output and pepsin activity. Howerver, the role of cysteamine in the pathogenesis of duodenal ulcer has remained unknown. The aim of this study was to periodically evaluate the changes of aggressive and defensive factors in cysteamine induced rats.
    We investigated the impairment of the local duodenal blood flow by means of the H+ clearance technique. Further, we have measured gastric acidity and pepsin activity. In addition, organ distribution of 35S-cysteamine detected with liquid scintillation counter and autoradiography was investigated. The duodenal ulcer incidence at 12 and 24 hours was higher than at 3 hours and duodenal mucosal blood flow decreased significantly 30 minutes after cysteamine administration. Gastric acidity and pepsin activity were increased 3 hours after administration. The activity of 35S-cysteamine in the duodenum was the highest at 1 hour and high level activity was maintained for 24 hours. 35S-cysteamine-labelled cells were located to the mucosal layer accompanied with dilatation of lymph vessels and infiltration of inflammatory cells at 30 minutes and 1 hour.
    I would like to emphasize the correlation between the change of duodenal microcirculation and ulcer formation on the basis of 35S-cysteamine accumlation in the duodenum. In conclusion, it is suggested that impairment of duodenal microcirculation may be due to direct destruction of the blood vessels or due to release of vasoactive amine from the tissue at a secondary step following cysteamine administration. Further, it may contribute to ulcer formation in rats.
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  • Yoshio HOSHIHARA, Sohtaro FUKUCHI, Kazuo HAYAKAWA, Naoyuki YAMADA, Yuk ...
    1985 Volume 82 Issue 8 Pages 1853-1857
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The intensity of Argon laser induced fluorescence at the normal mucosa of the gastrointertinal tract is relatively weak at about 540 and 580nm, and strong at about 560 and 600nm. This mechanism is unknown. We report its mechanism and the possibility of its clinical application.
    By spraying epinephrine solution to the mucosa or strongly pushing the optical quartz fiber bundles for measurement against the mucosa, the intensity of the fluorescence increased and its spectrum showed a smooth declined slope probably due to no absorption of the fluorescence by blood in the tissue.
    We made a chamber of two pieces of slide glass and placed it on the fixed resected specimen of the stomach, in which the mucosal layer was removed and the muscularis mucosa was exposed. When the chamber was filled with distilled water, Argon laser induced fluorescence spectrum on it showed a smooth curve. But when it was filled with hemoglobin solution, the spectrum pattern was similar to that of the normal mucosa.
    The partial absorption of the Argon laser induced fluorescence by hemoglobin resulted in the irregular fluorescence spectrum curve such as troughs at about 540 and 580nm and peaks at about 560 and 600nm.
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  • Hajime KUWATA, Kazuhiko ISHIHARA, Susumu OHARA, Haruya OKABE, Kyoko HO ...
    1985 Volume 82 Issue 8 Pages 1858-1864
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The effects of 17S, 20-dimethyl-6-oxo PGE1, methyl ester (PGE1, ) and 15(R)-15-methyl PGE2 (PGE2) on the quantitative changes of gastric mucosal glycoproteins as well as the degree of macroscopical mucosal lesion induced by 70% ethanol in rat were studied.
    The mucus glycoproteins in gastric mucosa were isolated from the corpus region after single oral administration or previous administration of PGE1, or PGE2.
    1) Previous administration (1 hr) of PGE1, or PGE2 markedly reduced the severity of macroscopical mucosal damage and prevented the quantitative reduction of gastric mucus glycoproteins induced by ethanol. However, the rate of the prevention was limited to about 90% of the control. The result suggests the presence of surface mucosal damage without obvious macroscopical lesion.
    2) Single administration of PGE1, or PGE2 occurred no significant change of mucus glycoprotein content compared with that of control.
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  • Takeo YAMANAKA, Yukio YOSHIDA, Norio UENO, Ken KIMURA, Takako YAMAGUCH ...
    1985 Volume 82 Issue 8 Pages 1865-1874
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Diagnosis of the degree of vertical invasion of gastric cancer by means of endoscopic ultrasonography is described, together with some problems.
    At present, the gastric cancer is echogenically divided into the following four groups according to the degree of invasion: a group of the invasion is so far limited to the mucosa (m-cancer group), a group of the invasion has spread to the submucosa (sm-cancer group), a group of the invasion has spread to the musclaris propria or subserosa (pm•ss-cancer group) and a group of the invasion has reached to the serosa (s-cancer group).
    In the present study, diagnostic accuracy of the degree of vertical invasion by endoscopic ultrasonogram is 87% (13/15 cases) in the m-cancer group, 83% (10/12 cases) in the sm-cancer group, 84% (16/19 cases) in the pm•ss-cancer group and 100% (4/4 cases) in the s-cancer group.
    The most common mis-diagnosis resulted from the limits of the ultrasonogram, i.e. the lesions of cancer and ulcer scars can not be differentiated in some cases, and the slight invasion of cancer cells can also not be detected ultrasonographically.
    Anyway, detailed information on the degree of vertical invasion of gastric cancer, which can not be determined by X-ray or ordinary endoscopy, can easily be obtained by means of endoscopic ultrasonography.
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  • Kazuo TARAO, [in Japanese], [in Japanese], Akira SAKURAI, Kazuhiro HAY ...
    1985 Volume 82 Issue 8 Pages 1875-1883
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Recently, it was demonstrated that a significant proportion of the non-sporing anaerobes, especially Bacteroides spp., produces urease. Since these organisms account for more than 99% of the total faecal flora, it was suggested that the gram-negative anaerobes make a major contribution to the generation of ammonia in hepatic encephalopathy in liver cirrhosis, and that minor components such as aerobic gram negative bacilli would be insignificant. To make clear of the problem, we administered a nonabsorbable antibiotics, vancomycin hydrochrolide, which is effective against anaerobic but ineffective against aerobic gram negative rods, to four patients with hepatic encephalopathy repeatedly, and studied the changes in clinical symptoms, blood ammonia, EEG and in faecal flora. With the administration of vancomycin, clinical symptoms of encephalopathy were improved, blood ammonia was decreased, and EEG was improved. These improvements were followed by a decrease in number of anaerobic gram-negative rods, especially Bacteroides. Besides, almost no change was observed in number of aerobic gram negative rods. Changes in blood ammonia level corresponded very well with changes in the number of anaerobic Bacteroides. It was concluded that the major contributor to the ammonia production in hepatic encephalopathy in cirrhotics was the Bacteroides species.
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  • Masao OMATA, Osamu YOKOSUKA, Junko MORI, Osamu YAMANAKA, Yasuhisa MATS ...
    1985 Volume 82 Issue 8 Pages 1884-1888
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Three hundred and twenty three HBsAg seropositive patients were tested for delta infection. Four (1.2%) were positive for delta antibody. None of them was I.V. drug users and had blood transfusion. HBV DNA was suppresed both in the serum and the liver in two patients. Hepatocellular degeneration, and spotty necrosis were prominent in 2 of 3 patients who were biopsied.
    These data indicates delta infection previals, although low in frequency, among"ordinary" Japanese HBsAg carriers.
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  • Osamu NISHIDA, Fuminori MORIYASU, Takefumi NAKAMURA, Nobuyuki BAN, Ken ...
    1985 Volume 82 Issue 8 Pages 1889-1893
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We measured the cross-sectional area and blood flow velocity of the superior mesenteric vein in 20 normal subjects, 20 patients with chronic hepatitis and 20 patients with liver cirrhosis. The cross-sectional area was 1.13±0.54cm2 in the liver cirrhosis patients, 0.72±0.23cm2 in the normal subjects and 0.83±0.34cm2 in the chronic hepatitis patients. There was a statistically significant difference between the liver cirrhosis patients and normal subjects (p<0.01). Blood flow velocity was 14.1± 3.7cm/sec in the liver cirrhosis patients, 14.8±3.9cm/sec in normal subjects and 13.4±3.1cm/sec in chronic hepatitis. There was no statistically significant difference between these groups. The ratio between the major axis and minor axis was 1.21±0.12 in liver cirrhosis, 1.36±0.18 in normal subjects and 1.31±0.29 in chronic hepatitis. There was a statistically significant difference between the liver cirrhosis patients and normal subjects. We assumed that the change in cross-sectional shape was due to elevation of the portal venous pressure.
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  • Kazuro YOSHIDA, Atsushi OZAWA
    1985 Volume 82 Issue 8 Pages 1894-1900
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Bacterial endotoxins in plasma play an important role in clinical course and prognosis of hepatobiliary disease, but the satisfied method for the quantitaion of endotoxins has not been established. We have developed a sensitive method to determine endotoxins by the change of fluorescent polarization using amebocyte lysate labeled with a fluorescent probe, fluorescamine. By this method endotoxins were detected at the concentration of 1×10-7μg/ml in physiological saline solution and in distilled water. Endotoxins in low concentration of glucose solution and of amino acid solution were able to be determined. Endotoxins in high concentration of glucose solution could be determined by diluting with physiological saline solution. Endotoxins added to human plasma were almost completely recovered by using the chloroform extraction and the freeze-dry treatment. However, plasma which showed positive limulus test could not be assayed in some samples by this method.
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  • Akihiko MASU, Wataru TAKAHASHI, Noriyoshi SUZUKI, Junichi GOTO, Toshio ...
    1985 Volume 82 Issue 8 Pages 1901-1907
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    A precise and sensitive method for the separation and determination of bile acid glucuronides in human bile is described. Bile acids in human bile were hydrolyzed by cholylglycine hydrolase and separated into the unconjugated, glucuronide, and sulfate fractions by ion-exchange chromatography on a newly developed lipophilic gel, piperidinohydroxypropyl Sephadex LH-20. Bile acid glucuronides were then hydrolyzed by the β-glucuronidase preparation from E. coli, and the free bile acids were determined by the enzymatic method. This new assay system requires only 1 to 10μl of human bile and is applicable to the quantitation of bile acid glucuronides in bile with satisfactory precision and reproducibility.
    Using this method, the bile acid glucuronide levels in gallbladder bile were measured in clinical cases. The amounts of glucuronides relative to total bile acids were 1.1±0.4% and 1.8±0.6% in patients with and without cholecystolithiasis, respectively. The composition ratio of glucuronides in lithocholates was larger than in total bile acids, while that in cholates was smaller.
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  • Norio UENO, Takeo YAMANAKA, Tetsuo KASANO, [in Japanese], Hiroyoshi AR ...
    1985 Volume 82 Issue 8 Pages 1908-1913
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The ultrasonographic feature of the gallbladder was chronologically studied in 42 patients with acute liver damage, and the following results were obtained. When the ultrasonographic feature of the gallbladder was classified into 4 types according to it's pathological changes of pattern, the majority of the gallbladders of the patients in acute stage belonged to more than type 2. When compared with liver function tests such as GOT, GPT, total bilirubin, prothrombin consumption test and serum albumin levels, these parameters deteriorated as the type advanced up to type 4. Additionally, the ultrasonic follow up of the gallbladder was found useful for the differetial diagnosis of fulminant hepatitis from other acute liver damage, and for the judgement of the stage and prognosis of hepatitis.
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  • Tatuhiko TANAKA, Masahiro YAMAMOTO, Shuichi OKUMURA, Harumasa OYANAGI, ...
    1985 Volume 82 Issue 8 Pages 1914-1923
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Diagnostic significance of measurement of serum pancreatic trypsin inhibitor (PSTI) by radioimmunoassay (RIA) was studied on canine experimental acute pancreatitis.
    The PSTI was purified from canine pancreatic juice and a reliable RIA system for canine PSTI has been developed, serum PSTI level in healthy dogs was measured to be 4.3±4.0ng/ml (mean±SD). Using this RIA, the changes in the serum PSTI were evaluated in the canine experimental model. It began to show a high level immediately after development of pancreatitis (highest value 50.8± 54.0ng/ml) and maintained the high level in the necrotizing pancreatitis.
    In contrast, the edematous pancreatitis of mild model of acute pancreatitis had a lower level of the PSTI. Substantial difference was observed between edematous and necrotizing pancreatitis.
    In addition, the PSTI level remained signifivantly higher in the group of dead animals than that in the group of survivors. It is suggested that the measurement of the time course changes of the serum PSTI was useful not only for evaluating the severity but also the prognostic interpretation in acute pancreatitis.
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  • Kazuichi OKAZAKI, Yasuro YAMAMOTO
    1985 Volume 82 Issue 8 Pages 1924-1931
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    To determine the significance of manometric pressure of the pancreatic duct, duodenoscopic measurements of the papillary sphincter zone and pancreatic main duct in control subjects (n=20) and patients with chronic pancreatitis (n=20), and the effects of exogenous glucagon or secretin were studied. There was not any significant difference between control subjects and patients with chronic pancreatitis without papillitis in the motility of the sphincter of Oddi. The pancreatic main ductal pressure in patients with chronic pancreatitis (54.5±29.9mmHg) was significantly higher than that in control subjects (16.2±8.7mmHg). In patients (n=6) with minimal-change chronic pancreatitis (MIP) without dilatation of pancreatic main duct, patients (n=10) with moderate-change chronic pancreatitis (MOP) and patients (n=4) with advanced-change chronic pancreatitis (ADP), the pancreatic main ductal pressure showed 51.8mmHg, 62.8mmHg and 37.8mmHg on average, respectively. The viscosity of pure pancreatic juice, which appeared to be one of constituents of resistance to secretion of pancreatic juice, in patients with chronic pancreatitis (5.8 centi-Poise: cP) in the basal secretory phase was significantly higher than that in control subjects (1.61 cP). But there was not any relationship between pancreatic main ductal pressure and viscosity of pure pancreatic juice. These data lead to the hypothesis that increased pancreatic ductal pressure in patients with chronic pancreatitis without papillitis appeared not to be due to papillary dysfunction, but to increased viscosity or other unknown factors; for example, basal secretory pressure, and then initially increased pancreatic ductal pressure may induced dilatation of the pancreatic main duct. Moreover, glucagon or secretin has a reducing effect on pancreatic ductal pressure in patients with chronic pancreatitis.
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  • Takefumi NAKAMURA, Fuminori MORIYASU, Nobuyuki BAN, Osamu NISHIDA, Ken ...
    1985 Volume 82 Issue 8 Pages 1932-1939
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    For the purposes of clinical application of an ultrasonic B-mode combined pulsed Doppler duplex system (a duplex system) and quantitative measurement of the blood flow in the abdominal arteries, we investigated the canine superior mesenteric artery and carotid artery using the duplex system and an electro-magnetic flowmeter simultaneously. We then tried to measure quantitatively the blood flow in the human superior mesenteric artery (SMA), splenic artery (SPA), and common hepatic artery (CHA) using the duplex system.
    In the canine superior mesenteric artery and carotid artery, the time-average blood flow volume was calculated as the product of the time-average blood flow velocity, which was gained from the time-integral of the maximum Doppler shift frequency, multiplied by the time-average cross-sectional area obtained from the M-mode. The time-average blood flow volume values gained using the Doppler duplex system and the electro-magnetic flowmeter showed a tight linear correlation (r=0.98), and the ratio between the values was 1:0.62.
    The blood flow volume in healthy adults was as follows; SMA 478±166ml/min, SPA 370± 181ml/min, CHA 254±131ml/min (mean±S.D.). The mean pulsatility index of each artery was 0.85 in SMA, 0.75 in SPA, and 0.79 in CHA. The pulsatility index of the SMA was significantly higher than that of either the SPA or CHA (p<0.001), and this indicated that the blood flow resistance of the SMA is larger than that of either the SPA or CHA.
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  • Saeko KAMEYA, Tsutomu WATANABE, Hideo SHIBATA
    1985 Volume 82 Issue 8 Pages 1940-1943
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Akira SUGITA, Tsuneo FUKUSHIMA, Masaru KAWAMOTO, Hiroshi SUWA, Akira K ...
    1985 Volume 82 Issue 8 Pages 1944-1947
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Report of two cases
    Yukiya YOSHIDA, Mitsuyo HASHIMOTO, Naoyuki YAMADA, Kazuo HAYAKAWA, Sot ...
    1985 Volume 82 Issue 8 Pages 1948-1953
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yutaka SASAGAWA, Hiroyuki ODA, Kazuaki KAMISAKA, Hidenori MAEZAWA, Rei ...
    1985 Volume 82 Issue 8 Pages 1954-1959
    Published: 1985
    Released: December 26, 2007
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  • Hiroshi TSUJI, Koichiro MURAI, Eiji KAJIWARA, Kimihiro AKAGI, Mikihisa ...
    1985 Volume 82 Issue 8 Pages 1960-1964
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yoshihisa SHIZAWA, Yoshiko KOIKE, Noboru KASAI, Michihiro SUZUKI, Shin ...
    1985 Volume 82 Issue 8 Pages 1965-1970
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Masato NAGINO, Shigehiko KONDOH, Kazuo TAKAYANAGI
    1985 Volume 82 Issue 8 Pages 1971-1976
    Published: 1985
    Released: December 26, 2007
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  • Toshio FUJIKI, [in Japanese]
    1985 Volume 82 Issue 8 Pages 1977
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Masahiro NAKAMURA, [in Japanese], [in Japanese], [in Japanese], [in Ja ...
    1985 Volume 82 Issue 8 Pages 1978
    Published: 1985
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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