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Takeshi OKUBO, Yozo WATANABE, Tsutomu KIDOKORO, Kazuhiko ISHIHARA, Kyo ...
1986 Volume 83 Issue 6 Pages
1111-1116
Published: 1986
Released on J-STAGE: December 26, 2007
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Quantitative changes of gastric mucosal glycoproteins were examined during the process in restraint and water immersion stress in rats. The stomaches were isolated before stress loading, 2, 4, 6 and 8 hours after stress loading, and Ulcer Index (U.I.) was calculated. U.I. showed a trend to gradually increase after stress loading, but no more increase was noted after 6hrs, or later.
The glycoprotein in mucosa of the corpus of stomach was isolated and analysed quantitatively. The result revealed that hexose level of 5.71±0.24mg/g of dry tissue weight before stress loading was significantly decreased after 2hrs, and decreased to 75% after 6hrs and 68% after 8hrs, respectively. Changes of carbohydrate composition in gastric mucosal glycoproteins were examined during stress loading. The result showed that galactose and fucose were decreased.
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Koichi UESAKA
1986 Volume 83 Issue 6 Pages
1117-1125
Published: 1986
Released on J-STAGE: December 26, 2007
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Duodenal mucosal changes induced in rats by subcutaneous injection of cysteamine were observed by stereo, light, and electron microscopy in order to clarify the mechanism of ulcer formation. Initial lesions revealed by electron microscopy were nonspecific degenerations of absorptive epithelial cells of the duodenum 2 hours after administration of 200mg/kg body weight of cysteamine. Microvilli and mitochondria of the injured cells were destroyed. Nuclei lost parachromatin and the cytoplasm became electron-lucent. Such degenerated absorptive epithelial cells were most prominent at the tips of villous folds and exfoliated in progress of cell injury. Thereafter, villous cores were denuded, and erosion and ulcer developed. Endothelial cells of small vessels in lamina propria were scarcely injured. Duodenal mucosal damage was not always accompanied with decrease of mucin in Brunner glands. Therefore, initial lesions of duodenal ulcer seemed to be caused by direct cytotoxic action of cysteamine on epithelial cells.
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With Special Reference to the Experimental Studies Using Germfree Rats
Hiromichi YAMAGUCHI
1986 Volume 83 Issue 6 Pages
1126-1134
Published: 1986
Released on J-STAGE: December 26, 2007
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To elucidate the role of Clostridium difficile in pseudomembranous colitis, germfree rats were monoassociated with a toxin-producing strain of C. difficile. Watery diarrhea was observed in all animals after monoassociation during several days and mortality rate were 20% in this group. No pseudomembrane formation was observed in both died and survived rats in this group. Furthermore, improvement of inflammatory changes in the intestine was observed during two weeks after monoassociation.
Ligation of the rectum was added to the monoassociated rats to make the situation of pooling feces. After a few days of the additional procedure, the pseudomembrane similar to the clinical cases was successfully observed at the oral portion of the colon above the rectal ligation. In addition, much more enterotoxin was measured in the ligated group than the non-ligated group.
From these results, it could be suggested that C. difficile have the potential role for producing pseudomembranous colitis. It was demonstrated in this series of experiment that the factors of producing pseudomembranous colitis were not only abnormaly increased bacteria but also increased enterotoxin caused by the stasis of intestinal content.
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Inhibition of Proliferation in Colonic Cancer with Indomethacin Treatment
Mitsumasa KOIKE, Saburo NAKAZAWA, Takayuki OZAWA
1986 Volume 83 Issue 6 Pages
1135-1142
Published: 1986
Released on J-STAGE: December 26, 2007
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In order to know the role of endogenous prostaglandins (PGs) in 1, 2-dimethylhydrazine (DMH) induced colonic cancer of rats, the effect of indomethacin (IND), which inhibits biosynthesis of PGs, on the promotion of the tumors was studied. On the 29th week of the treatment, the animals were sacrificed 48 hours after final administration of IND, and the resected colon was subject to the morphologic study and determination of PGs. By administering IND in the initial period of 16 weeks, the incidence of colonic cancers did not decrease significantly. On the other hand, the sum of the size of colonic cancers decreased distinctly during the latter period of 12 weeks by administration of IND. The levels of PGE in non-cancerous colonic mucosa decreased obviously in animals given IND in the latter 12 weeks. But PGE levels of colonic cancers did not change. There were no regularities of the PGF
2α levels in either non-cancerous mucosa nor cancer. The results suggests that the increase of the PGE levels promote proliferation of colonic cancer in DMH treated rats.
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Hiromasa OHTA
1986 Volume 83 Issue 6 Pages
1143-1152
Published: 1986
Released on J-STAGE: December 26, 2007
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Lymphoid cell subsets of the large bowel in ulcerative colitis (30 specimens) were studied by indirect immunoperoxidase staining using mouse anti-human monoclonal antibodies. In inflamed mucosa of ulcerative colitis, the ratio of Leu 3a+/Leu 2a+ had a tendency to be high in the lamina propria and the number of Leu 3a+ in the epithelium increased. The staining patterns of HLA-DR+ and Leu 10+ weresimilar between controls and ulcerative colitis, but in some cases with ulcerative colitis the epithelial cells of inflamed mucosa were stained with anti-HLA-DR antibody. IgG-, IgM-, IgE-, IgD- containg cells increased in number in the inflamed lamina propria of ulcerative colitis. IgE-, IgD- containing cells increased in number even in non-inflamed area of ulcerative colitis.
In conclusion, changes in immunoregulatory T cells, and increased antibody producing cells including IgE which is able to mediate an immediate hypersensitive reaction, were seen in ulcerative colitis.
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Motohide SHIMAZU, Haruo AOKI, Seiichi MITA, Makoto MIYAKITA
1986 Volume 83 Issue 6 Pages
1153-1160
Published: 1986
Released on J-STAGE: December 26, 2007
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We studied the effects of dopamine on hepatic and systemic hemodynamics during surgery in 15 patients with gallstones and normal liver function. Portal venous flow (PVF) and hepatic artery flow (HAF) were measured by local thermodilution and electromagnetic flowmeter, respectively.
Intravenous infusion of dopamine at a dosage of 3μg/kg/min caused a significant increase in PVF from a control value of 818±256ml/min (mean±SD) to 1018±361ml/min, but no significant change in HAF. Systemic circulation including cardiac output as determined by Swan-Ganz catheter showed no significant change. We conclude, therefore, that PVF increased independently of cardiac output. Portal venous pressure showed no apparent change, while portal venous resistance decreased significantly.
Dopamine also elevated portal venous PO
2, thereby causing an increase in oxygen supply to the liver combined with an increase in PVF.
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Characteristics of the Hepatocytotoxic Factors Released from the Activated Liver Adherent cells
Hiroko TSUTSUI, Keiji MIYAJIMA, Yasuhiro MIZOGUCHI, Yoshihide SAKAGAMI ...
1986 Volume 83 Issue 6 Pages
1161-1167
Published: 1986
Released on J-STAGE: December 26, 2007
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When heat-killed
Propionibacterium acnes (
P. acnes) and a small amount of endotoxin lipopolysaccharide (LPS) were injected intravenously into mice at a week's interval, most of the mice died ofmassive liver necrosis. When the adherent cells were isolated from the mouse liver 7 days after intravenous injection of
P. acnes and activated with LPS
in vitro, they released some hepatotoxic factors in their culture supernatants. This cytotoxic factors were fractionated by gel filtration using a Sephadex G-75 column and were suspected to be protein-natured substances having molecular weight 10 to 40K dalton. The cytotoxic activity was lost by trypsin digestion although none of RNase-, Dnase- and neuraminidase-treatments affected thir cytotoxic activity. These cytotoxic factors seemed to be rather heat-unstable materials, because their activity were almost destroyed during incubation for 30 minutes at 56°C.
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Hisao TAKAHASHI
1986 Volume 83 Issue 6 Pages
1168-1175
Published: 1986
Released on J-STAGE: December 26, 2007
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To evaluate if chronic ethanol administration modifies the hepatotoxicity of halothane, male Wistar strain rats fed chronically on liquid diet containing ethanol (36% of total calories) were anesthetized with 1% halothane under 10 or 35% O
2 concentration for 2 hours. Twenty-four hours after anesthesia, the liver of these animals showed multif ocal or patchy necrosis in the primarily centrilobular regions with polymorphonuclear and lymphocytic cell infiltration. Serum GOT and GPT activities in these animals were also elevated. Further, increase of lipid peroxide contents and decrease of glutathione peroxidase activities were demonstrated in both groups. The degree of these changes were stronger in 10% O
2 group than 35% O
2 group. Moreover, in 10% O
2 group, the animals showed decrease of hepatic reduced glutathione and increase of serum inorganic fluoride. These data suggest that reductive metabolism of halothane were potentiated by induction of microsomal drug metabolizing enzymes and enhancement of hepatic oxygen consumption produced on account of chronic ethanol administration, and that lipid peroxidation induced by the intermediate metabolite may cause the hepatic injury, because protective effect against lipid peroxidation were decreased in the liver on such conditions.
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Naoki MATSUO, Hajime OHISHI, Fusayuki NAKAGAWA, Toshihiko MURATA, Tets ...
1986 Volume 83 Issue 6 Pages
1176-1186
Published: 1986
Released on J-STAGE: December 26, 2007
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The prognosis was evaluated in 11 patients with hepatocellular carcinoma who survived for morethan 3 years after TAE. Nine of them are still alive. The longest follow up patients extended to 4 years and 4 months. Ten patients had a nodular type and six patients of them were less than 5cm. Portal obstruction was localized in the 2nd and 3rd branches. Lung metastsis was detected in one patient and daughter nodules were detected in seven patients. In two patients PTO was performed because of bleeding from esophago-cardial varices. The AFP level exceeded 100ng/ml in seven patients before TAE. In the Child classification, ten patients of them were classified as A. Gelatine sponge was used as an embolic material for all the cases, fuor patients of whom were combined with Lipiodol. Five patients of all performed repeated TAE at relatively short intervals, favorable prognosis was obtained. TAE that were combined with Lipiodol seemed effective for the management of collaterals that developed byfollowing TAE.
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Yukihiko NARUKI, Mamoru NISHINO, Yoshitsugu YOKOSAWA, Hiroki SHIMURA, ...
1986 Volume 83 Issue 6 Pages
1187-1191
Published: 1986
Released on J-STAGE: December 26, 2007
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Masahiro MIZUNO, Satoru MIYATA, Atsushi ARAKI, Hiroshi HAMASHIMA, Tosh ...
1986 Volume 83 Issue 6 Pages
1192-1198
Published: 1986
Released on J-STAGE: December 26, 2007
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A Case Report and Review of the Literature
Takeshi SEKINE, Yasuo SUDA, Kayako SHIMAMURA, Yukio NOGUCHI
1986 Volume 83 Issue 6 Pages
1199-1203
Published: 1986
Released on J-STAGE: December 26, 2007
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Tadashi IWAO, Takahumi HIND, Humihiko YAMASHITA, Hajima HISANAGA, Humi ...
1986 Volume 83 Issue 6 Pages
1204-1208
Published: 1986
Released on J-STAGE: December 26, 2007
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Toshihumi YOSHIOKA, Akiharu WATANABE, Rhoji USUMOTO, Tetsuya SHIOTA, M ...
1986 Volume 83 Issue 6 Pages
1209-1213
Published: 1986
Released on J-STAGE: December 26, 2007
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Yoshifumi HIGASHIMOTO, Seiichi HIMENO, Masanori KUROKAWA, Yasuhisa SHI ...
1986 Volume 83 Issue 6 Pages
1214-1220
Published: 1986
Released on J-STAGE: December 26, 2007
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Keiichi KUBOTA, Hiroshi KAWAI, Yoshikura HARAGUCHI, Toshihisa TAKAHASH ...
1986 Volume 83 Issue 6 Pages
1221-1224
Published: 1986
Released on J-STAGE: December 26, 2007
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[in Japanese], [in Japanese]
1986 Volume 83 Issue 6 Pages
1225-1227
Published: 1986
Released on J-STAGE: December 26, 2007
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Masao ICHINOSE, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
1986 Volume 83 Issue 6 Pages
1228
Published: 1986
Released on J-STAGE: December 26, 2007
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Shigetoshi FUJIYAMA, [in Japanese], [in Japanese], [in Japanese], [in ...
1986 Volume 83 Issue 6 Pages
1229
Published: 1986
Released on J-STAGE: December 26, 2007
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Yukio YOKOI, [in Japanese], [in Japanese], [in Japanese], [in Japanese ...
1986 Volume 83 Issue 6 Pages
1230
Published: 1986
Released on J-STAGE: December 26, 2007
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Namiki IZUMI, [in Japanese], [in Japanese], [in Japanese], [in Japanes ...
1986 Volume 83 Issue 6 Pages
1231
Published: 1986
Released on J-STAGE: December 26, 2007
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Mutsunori SHIRAI, [in Japanese]
1986 Volume 83 Issue 6 Pages
1232
Published: 1986
Released on J-STAGE: December 26, 2007
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Shuichi NOJIRI, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
1986 Volume 83 Issue 6 Pages
1233
Published: 1986
Released on J-STAGE: December 26, 2007
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