Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 84 , Issue 3
Showing 1-24 articles out of 24 articles from the selected issue
  • In Association with Acetylcholine Release
    Mitsuharu NAKAMOTO, Tomoaki URAKAWA, Yoichi SAITOH
    1987 Volume 84 Issue 3 Pages 653-660
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Inhibitory mechanism of dopamine on rat gastric motility was investigated in association with acetylcholine (ACh) release. Vagotomy or sympathectomy had no effect on the inhibitory action of dopamine, indicating that the site of action of dopamine is in the gastric wall. Electrical transmural stimulation induced a neurogenic muscular contraction and [3H] ACh release from the muscle strips of rat stomach preloaded with [3H] choline. Dopamine at the concentration of 10-4M inhibited significantly by 41% the [3H] ACh release induced by electrical transmural stimulation.
    The inhibitory effect of dopamine was significantly antagonized by domperidone, but not by adrenergic blocking agents such as phentolamine or propranolol.
    These results indicate that dopamine plays an important role in the regulation of gastric motility by reducing ACh release through specific dopamine receptor located on the postganglionic cholinergic neurons.
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  • Taeko MIYAHARA
    1987 Volume 84 Issue 3 Pages 661-669
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Various classes of immunoglobulin (Ig) in sera from 26 patients with ischemic colitis (IC) and 40 patients with antibiotic associated colitis (AAC) were measured and various classes of Ig containing cells and secretory component (SC) were investigated using immunofluorescence technique.
    The mean value of serum IgE level was significantly increased in irregular ulcer type (IU type) than that of longitudinal ulcer or erosion type (LUE type), AAC, and normal control. The number of IgE containing cells in the biopsy specimens of IU type was much higher than that of LUE type. AAC, and normal control.
    These findings suggest that the immediate hypersensitivity might play one of the important part of pathogenesis in IU type.
    In the patients with AAC, the percentage proportions of IgA containing cells and stainability of SC in the inflammed mucosa and normally appeared mucosa were significantly decreased. These results reveal the disturbance of the local defense mechanism concerning with IgA secretory-immunoglobulin system over the total colon. Then it is suggested that in the patient with AAC the entire colonic mucosa might have a possibility to be fallen into inflammation.
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  • Hiroko TSUTSUI, Yasuhiro MIZOGUCHI, Keiji MIYAJIMA, Yoshihide SAKAGAMI ...
    1987 Volume 84 Issue 3 Pages 670-674
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We have previously shown that acute hepatic failure is easily induced in animals by the intravenous injection of heat-killed Propionibacterium acnes and lipopolysaccharide endotoxin at a week's interval. And hepatocytotoxic factor(s) produced by the fully activated liver adherent cells might involve these liver injuries. Gomisin A a lignan component from Shizondra chinensis, blocked the induction of such severe liver damages.
    In this report, we tried to clarify the mechanism by which this medicine protected the mice from being suffered of liver injuries. We examined in vitro the effect of gomisin A both on hepatocytotoxic activity of liver adherent cells and on liver parenchymal cells. Firstly, gomisin A suppressed releasing of cytotoxic factor from the activated liver adherent cells. Secondly, it also could keep liver parenchymal cell membrane stable even in the presence of cytotoxic substances.
    These results suggested that gomisin A might inhibit the induction of acute hepatic failure in our system, partialy because of blocking release of hepatocytotoxic factor and of stabilizing membrane of liver parenchymal cells.
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  • Hidetsugu SAITO, Toshio MORIZANE, Akiko IINO, Kanji TSUCHIMOTO, Yoshik ...
    1987 Volume 84 Issue 3 Pages 675-683
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We developed a murine monoclonal antibody, designated H2, which reacts specifically with human hepatocytes. In the previous study it was demonstrated by immunoperoxidase technique that the reactivity of this H2 monoclonal antibody was restricted to human hepatocytes. We have proposed that the antigen defined by this monoclonal antibody is designated "human liver-specific antigen 1 (HLSA 1)". We report here the partial characterization of HLSA1. HLSA1 was thought to make a large conglomerate consisting of a unit protein with 15000 dalton molecular weight. HLSA1 was stable at 4°C or 37°C but destroyed by incubation at 60°C for 60min. It was stable at pH 8.0 but destroyed at pH 4.0 and also destroyed by trypsin treatment. HLSA1 was shown to have affinity to some lectins and this result suggest that HLSA1 is a glycoprotein which possesses carbohydrate moieties. Moreover, it was demonstrated by immunoelectron microscopy that HLSA1 was distributed in the perinuclear space, rough endoplasmic reticulum and cell membrane of human hepatocytes. This suggests that HLSA1 is a cell membrane-associated protein.
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  • Hiroko MATSUDA, Masahiro MATSUMOTO, Noriaki MIZUSHIMA, Katsutoshi TAMA ...
    1987 Volume 84 Issue 3 Pages 684-689
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Changes of liver functions associated with endoscopic sclerotherapy (EST) was investigated in 50 cirrhotic patients with remarkable esophageal varices. The indices used for the evaluation of hepatic function was the increase of total bilirubin (ΔT. Bil.) in cases who were treated by paravariceal injection of sclerosant and the ratio between the increase of total bilirubin and the increase of LDH (I.I.) in cases treated by intravariceal injection of sclerosant. In a group of patients with preserved hepatic reserve (Group I, 28 cases), Δ T.Bil. was 0.44±0.37mg/dl and I.I. 0.28±0.19, respectively. In contrast, in patients who showed diminished hepatic reserve prior to EST (Group III, 8 cases), a significant deterioration of liver function was observed after EST (Δ T. Bil. 1.65±0.68mg/dl, I.I. 1.01±0.70, p<0.01). Among the patients who revealed hepatic reserve between that of Group I and Group III (Group II, 14 cases), the extent of the deterioration of liver function after EST was also between Group I and III. Recovery of increased serum bilirubin after EST to pre-treatment level was delayed in Group III.
    It is concluded that the deterioration of liver function can occur after EST among cirrhotic patients especially those with diminished hepatic reserve prior to EST.
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  • Hironobu OHTA, Minoru NOMOTO, Toshihiko OZAKI, Tomoteru KAMIMURA, Fumi ...
    1987 Volume 84 Issue 3 Pages 690-697
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Clinical investigation was carried out in twenty cases with ruptured hepatocellular carcinoma during 1979-1986. The rupture occured spontaneously in 12 patients, but 8 patients had the cause or predisposing factor of rupture such as endoscopy, defecation, infusion of adriamycin. The initial symptoms were the sudden on set of pain, shock and muscular defence, ileus, vomiting and fever. Transcatheter arterial embolization (TAE) was performed in five patients. Two among five patients showed longterm survival, but the others worsened rapidly after TAE and died within short period.
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  • Motonobu SUGIMOTO
    1987 Volume 84 Issue 3 Pages 698-705
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The major glutathione S-transferases have been purified from three apparently normal human livers: two obtained at surgery and one at autopsy. Purification was by sequential gel filtration, glutathione affinity chromatography, and chromatofocusing.
    All three livers exhibited the same major cationic transferase peaks from chromatofocusing at pH 9.0 and 8.7 (designated C1 and C2, respectively) and several (2-3) minor peaks. The major anionic form (designated A1) from two livers eluted at the same position (pH 5.4), and the major form from the third liver (designated N1) eluted near neutral (pH 6.8). The transferase from erythrocytes eluted at pH 4.6.
    In SDS-polyacrylamide gel electrophoresis, C1, C2 and Al showed the same single subunit (25, 000) whereas N1 was different (26, 000). The erythrocyte enzyme had a smaller subunit (23, 500). The amino acid composition and substrate specificity were very similar in C1, C2 and A1 whereas those of N1 were different.
    Urea/SDS-polyacrylamide gel electrophoresis resolved the apparent single subunit of C1, C2 and A1 into two distinct subunits. C1 was a homodimer of the faster migrating subunit; A1 was a homodimer of the slower migrating subunit; and C2 was a heterodimer of those two subunits.
    The results confirm; (1) large interindividual differences in content of each form; (2) in contrast to previous reports, close similarity in subunit, amino acid composition and substrate specificity between cationic and anionic forms; and (3) a new subunit heterogeneity in which the cationic and anionic transferases are homo or heterodimers of two distinct subunits.
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  • Osamu NISHIDA, Fuminori MORIYASU, Takefumi NAKAMURA, Nobuyuki BAN, Tak ...
    1987 Volume 84 Issue 3 Pages 706-711
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We compared the abdominal hemodynamic state in idiopathic portal hypertension (IPH) with that in liver cirrhosis with splenomegaly (LCS). Eleven IPH and 15 LCS cases were studied. The proper hepatic, right hepatic, left hepatic and superior mesenteric arteries were wider in LCS than IPH patients. There was no difference, however, in the width of the splenic srtery. Using an ultrasonic duplex system, we found a tendency for the superior mesenteric venous blood flow volume to be greater in LCS than in IPH. No other differences in blood flow parameters could be detected, and there was no difference in the oxygen pressure of the portal vessels in each of the diseases. However, the difference between portal and wedged hepatic venous pressures was found to be greater in IPH than in LCS.
    There was a statistically significant difference between the arterial hemodynamics in IPH and LCS, and in the site of vascular resistance within the liver, but not in portal hemodynamics.
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  • Nobuhiro TOKIKUNI, Shujiro TAKASE, Akira TAKADA
    1987 Volume 84 Issue 3 Pages 712-718
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The activities of serum glutamate dehydrogenase (GDH), ornithine carbamyl transferase (OCT) and mitochondrial GOT (m-GOT), which are liver mitochondrial enzymes with different sublobular localization, were determined simultaneously in patients with drug induced liver injury and acute viral hepatitis to elucidate the usefulness of the determinations of these enzymes in the diagnosis of drug induced liver injury. Serum GDH activity was higher in drug induced liver injury than in acute type A hepatitis, and conversely, serum OCT activity was lower. Serum m-GOT levels were significantly lower in drug induced liver injury than in various types of viral hepatitis. The ratios of m-GOT to cytosolic GOT, however, were significantly higher in drug induced liver injury than in various types of viral hepatitis. In almost all cases in drug induced liver injury, the ratios of GDH/OCT were high and the values were nearly 1.0. In acute viral hepatitis, only 3 cases of NANB hepatitis showed higher ratios over 1.0. These results suggest that GDH/OCT ratios accurately reflect the sublobular distribution of hepatocellular damage, and that the determination of GDH/OCT ratios is a useful parameter in the diagnosis of drug induced liver injury.
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  • Yasuhiro MIZOGUCHI, Keiji MIYAJIMA, Mizuho KITAMURA, Hiroko TSUTSUI, H ...
    1987 Volume 84 Issue 3 Pages 719-725
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Although lymphocyte transformation and lymphokine production have been frequently used for the identification of the causative drug in patients with drug-induced allergic hepatitis, these immunological parameters are not always positive in all patients. This may be reflected in the population of suppressor cells. In order to augment these immunological reactions, we devised a method in which indomethacin was added to the lymphocyte culture simultaneously with antigen (drug and carrier). In patients who did not show any positive lymphocyte transformation or lymphokine (cholestatic factor) production by stimulation with the causative drug in the presence of the suitable carrier, the addition of indomethacin was found to enhance the immunological responses. That is, lymphocyte transformation by the causative drug was significantly induced.
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  • With Special Reference to the Effect of High Molecular Substances
    Hisashi YAMAGUCHI, Fumiaki SHINYA, Wataru TAKAHASHI, Noriyoshi SUZUKI
    1987 Volume 84 Issue 3 Pages 726-732
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We analyzed both calcium concentration in bile and inhibitory effect of bile on β-glucuronidase activity in order to study the regulatory factors for the precipitation of calcium bilirubinate. Hepatic bile samples with and without gallstone disease was used in this study. The centrifuged supernatants of each bile samples were divided into two fractions using ultrafiltration technique (fraction molecular weight 1, 000). Both fractions and the supernatants were tested for the inhibitory effect on bacterial β-glucuronidase activity and the calcium concentration. Almost all inhibitory substances of β-glucuronidase existed in a high molecular phase of bile. In the bile with calcium bilirubinate stones, both inhibitory effect on bacterial β-glucuronidase and calcium trapping capacity were significantly lower than in the bile with mixed stone and without gallstone.
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  • Kazuichi OKAZAKI, Yasuro YAMAMOTO, Soichi KAGIYAMA, Yoshiya SAKAMOTO, ...
    1987 Volume 84 Issue 3 Pages 733-740
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Duodenoscopic measurements of pressures in the papillary sphincter zone and pancreatic main duct in 20 control subjects and 31 patients with chronic pancreatitis including 10 cases of alcoholic chronic pancreatitis and 21 cases of idiopathic chronic pancreatitis.
    The pancreatic main ductal pressure was significantly higher in the patients with alcoholic (55.7± 28.9mmHg) or idiopathic chronic pancreatitis (44.5±25.8mmHg) than in the controls (16.2± 8.7mmHg), but there was no significant difference between patients with alcoholic and idiopathic chronic pancreatitis. There was no significant difference between control subjects and patients with chronic pancreatitis in the peak and basal pressure of the sphincter of Oddi. Nevertheless, the basal pressure and the frequency of the papillary sphincter waves in patients with alcoholic chronic pancreatitis are higher than those in normal subjects, but there were no significant differences between patients with idiopathic pancreatitis and normal subjects.
    These data suggest that increased pancreatic ductal pressure in patients with alcoholic pancreatitis may be due to papillary dysfunction.
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  • Noriaki NAKAMURA, Masaki HIROTA, Kiyoyasu FUKUSHIMA, Shiro FUNATSU, Ka ...
    1987 Volume 84 Issue 3 Pages 741-748
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Paraffin embedded 64 pancreatic tissues including 26 cases of resectable pancreatic carcinomas, 12 cases of probe biopsy, 9 cases of operated chronic pancreatitis, and 17 cases of biopsied normal pancreas were examined by indirect immunoperoxidase staining. ABO and Lewis blood type were determined by hemagglutination test, and ABO blood type in saliva was done by hemagglutination inhibition assay. Monoclonal antibodies recognizing Lea, sialylated Lea, Lex, and sialylated Lex were produced by the immunizations of mice with colon cancer line, mixture of colon cancer lines, HL60, and the extract of stomach cancer tissue, respectively.
    In non-cancerous tissue of chronic pancreatitis and normal pancreatic tissues, small pancreatic ducts adjacent to acini were stained by anti-A, B, and H antibodies, and its staining patterns were compatible to individual blood type. Staining of pancreatic ducts with anti-Lea and antisialylated Lea antibodies was observed in 92.3% of tested samples. Lewis blood type of negatively stained individuals was Lewis (a-b-). Lex was observed in only 15.4% of cases, but sialylated Lex was not seen at all in non-cancerous tissues.
    On the contrary, in 38 cancerous tissues, the deletion of ABH blood type antigen was seen in 21 to 38 (55.8%) cases, and incompatible blood type antigen was not detected. Furthermore, the positively of tumor-associated antigens was as follows: 33 cases (86.8%) in sialylated Lea, 6 cases (15.8%) in Lex and 28 cases (73.7%) in sialylated Lex.
    From these studies, the following conclusions were obtained:
    1) In non-cancerous pancreatic ducts, blood type compatible carbohydrate antigen is synthesized.
    2) In pancreatic cancers, blood type carbohydrates are synthesized incompletely, followed by the deletion of the antigens.
    3) This deletion of blood type carbohydrates in pancreatic tissues is significantly observed in nonsecretor cases.
    4) In pancreatic tissues, sialylated Lea is tumor-associated antigen. However, sialylated Lex is tumor-specific antigen and could be must useful tumor marker.
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  • Toshihiko IIJIMA, Masaji NAMBU, Takuro MIYAMURA
    1987 Volume 84 Issue 3 Pages 749-753
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Mikiko SHIGEMITU, Koichi JYOYA, Ryosaku HARADA, Ryusuke SYUTO, Syuji I ...
    1987 Volume 84 Issue 3 Pages 754-757
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yumiko KAMOGAWA, Atuko KANEKO, Masahiko TOMIMATU, Tuyoshi KURIHARA, Et ...
    1987 Volume 84 Issue 3 Pages 758-762
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Kunio SUZUKI, Teruhisa AKEYAMA, Masaki TOGAWA, Masaaki SUZUKI, Tsutomu ...
    1987 Volume 84 Issue 3 Pages 763-767
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hirohiko SAKAMOTO, [in Japanese], [in Japanese], [in Japanese], [in Ja ...
    1987 Volume 84 Issue 3 Pages 768
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Takeo ASADA, [in Japanese], [in Japanese], [in Japanese]
    1987 Volume 84 Issue 3 Pages 769
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Tsuyoshi BANDO, [in Japanese], [in Japanese]
    1987 Volume 84 Issue 3 Pages 770
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Junichi SHODA, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1987 Volume 84 Issue 3 Pages 771
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Comparison with anti-estrogen treated liver
    Yasuo HIMENO, [in Japanese], [in Japanese], [in Japanese], [in Japanes ...
    1987 Volume 84 Issue 3 Pages 772
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Kiyohide KIOKA, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1987 Volume 84 Issue 3 Pages 773
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Satoru TAMURA, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1987 Volume 84 Issue 3 Pages 774
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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