Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 84 , Issue 6
Showing 1-22 articles out of 22 articles from the selected issue
  • The 1st Report
    Hiroyuki KARASAWA
    1987 Volume 84 Issue 6 Pages 1201-1210
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The inhibitory effect of omeprazole on histamine-, carbachol- and tetragastrin-stimulated gastric acid secretion was studied both in vivo and in vitro using guinea pig, being compared with that of H2-receptor antagonist. In vivo, omeprazole inhibited acid secretion induced by these three secretagogues. The inhibitory effect was found to be aproximately 11-17 times more potent than cimetidine on histamine stimulated gastric acid secretion. In vitro studies using isolated parietal cells, omerprazole dose dependently inhibited acid secretion induced by not only three secretagogues but also di-butyryl cyclic AMP, while H2-receptor antagonists did not inhibit di-butyryl cyclic AMP stimulated acid secretion. This suggests that omeprazole, different from H2-receptor antagonist, acts on the distal portion from the site of activation of cyclic AMP.
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  • Hideaki KOSEKI
    1987 Volume 84 Issue 6 Pages 1211-1218
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    This study on the effect of oxygenation under high pressure (OHP) on gastric mucosa and experimental gastric ulcer in rats showed that (1) in untreated rats, OHP decreased significantly pepsin activity, but there was no significant change in gastric mucosal blood flow, hexosamine contents in gastric mucosa, gastric motility, pH of gastric juice, and gastrin contents in gastric mucosa. (2) The appearance of indomethacin-induced gastric ulcer was significantly inhibited by OHP, but in the water immersion and restraint stress, there was no significant change of ulcer appearance between control and OHP groups. The mechanism by which OHP inhibited the indomethacin-induced ulcer seems that OHP restored significantly gastric mucosal blood flow decreased by indomethacin. However, there was no significant difference of hexosamine contents after indomethacin administration between OHP and control groups. Accordingly, it was thought that OHP was a safe therapeutic maneuver with no side effect and applicable to the treatment of gastric lesion caused by micro-circulatory disturbance.
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  • Hiroaki ISHIMARU, Kazumi WATANABE, Takahide MATSUDA
    1987 Volume 84 Issue 6 Pages 1219-1224
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Effects of peripheral blood mononuclear cells (MNC) of asymptomatic HBsAg carriers (ASC) on LIF (leucocyte migration inhibition factor) production of peripheral blood MNCs of B type hepatitis were studied by indirect LIF test which examined the influence of supernatants of co-culture of MNCs on leucocyte migration of healthy persons. Namely, LIF production of MNCs stimulated by HBsAg, was evaluated with indirect LIF test.
    The results of our tests were described below.
    1) Supernatants of cultures of addition of HBsAg to peripheral blood MNCs of patients with convalescence of B type acute hepatitis or B type chronic active hepatitis, inhibited migration of leucocytes of healthy persons. Peripheral blood MNCs of these cases positive for LIF test were supplied to indirect LIF test described below. But, supernatants of culteres of addition of HBsAg to peripheral blood MNCs of healthy persons, ASCs or patients with NANB type CAH, did not inhibit migration of leucocytes of healthy persons.
    2) The supernatants of co-cultures of B type hepatitis MNCs and HBsAg in addition of peripheral MNCs of ASC, released inhibition of leucocyte migration.
    3) The supernatants of co-cultures of B type hepatitis MNCs and HBsAg in addition of cimetidinine-pretreated peripheral blood MNCs of ASC, inhibited leucocyte migration.
    4) The supernatants of co-cultures of B type heaptitis MNCs and HBsAg in addition of peripheral blood MNCs of NANB type CAH, did not release the inhibition of leucocyte migration.
    5) The supernatants of co-cultures of NANB type hepatitis MNCs and HBsAg in addition of peripheral blood MNCs of ASC, hardly influenced the capacity of leucocyte migration.
    6) The supernatants of co-cultures of peripheral blood MNCs of chronic hepatitis with LIF test positive for PPD and PPD in addition of peripheral blood MNCs of ASC, inhibited leucocyte migration.
    These results suggested that HBsAg specific suppressor cells in peripheral blood MNCs of ASC existed and worked to suppress LIF production of peripheral blood MNCs stimulated by HBsAg.
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  • Minoru YASUHARA, Yoshiro MATSUDA, Akira TAKADA
    1987 Volume 84 Issue 6 Pages 1225-1232
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Serum immunoreactive prolyl hydroxylase β-subunit (IR β-PH) levels were measured using monoclonal antibodies in various liver diseases and the levels were compared with serum procollagen type III N-terminal peptide (Pro-III-N-P) leveles. In almost all cases of chronic hepatitis and liver cirrhosis, serum IR β-PH levels were significantly elevated and tended to increase parallel to the degree of activity of hepatitis. Serum Pro-III-N-P levels showed nearly the same changes as those of IR β-PH in chronic hepatitis and cirrhosis. However, serum Pro-III-N-P levels were increased in almost all cases of acute hepatitis and metastatic liver cancer, but serum IR β-PH levels were in the normal range in metastatic liver cancer and elevated in only half of the cases of acute hepatitis. Although the correlation between serum IR β-PH and Pro-III-N-P levels was statistically significant in all cases of liver diseases, significant correlation was observed in only a few deseases. These results indicated that serum IR β-PH reflected more selectively the synthesis of heaptic collagen than Pro-III-N-P and that serum IR β-PH is one of the most sensitive markers of the hepatic fibrogenesis.
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  • Takao YOSHIDA
    1987 Volume 84 Issue 6 Pages 1233-1243
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to examine the mechanism of increased cyclic GMP levels in noncancerous liver diseases such as liver cirrhosis (decompensated) and acute hepatitis (the initial stage), in situ perfusion experiments were tried in rat livers treated with D-galactosamine (Gal-N) intraperitoneally, as a model of acute hepatitis in the rat.
    Perfusate cyclic GMP levels at 24, 48, and 72 hours afrter Gal-N injection increased significantly compared with that of control. Carbachol-induced cyclic GMP production increased at 24, 48, 72 hours, especially at 72 hours after the treatment. The response caused by carbachol was absolutely inhibited by atropine. Increased 3H-thymidine uptake in the liver was also found at the same time, indicating a close-relationship between the cyclic GMP production and the liver regeneration. Insulin affected additively on cyclic GMP production in regenerated livers.
    These results indicate that cyclic GMP may play an important role in hepatic regeneration through the muscarinic receptor in the liver.
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  • Toshihiko KAWASAKI, Fuminori MORIYASU, Nobuyuki BAN, Takashi TAMADA, Y ...
    1987 Volume 84 Issue 6 Pages 1244-1250
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Phosphorus-31 magnetic resonance spectroscopy was used to assess the changes in hepatic high-energy phosphate metabolites in rabbits with obstructive jaundice. The rabbits, which had undergone operative ligation of the common bile duct, were studied using a 2.0 Tesla whole-body magnetic resonance imager.
    Comparison of the peak phosphorus signal values relative to α-ATP showed that the peak phosphodiester and γ-ATP values in the livers of the one-day-after-ligation group were significantly lower than those in the control group, and the peak phosphomonoester and phosphodiester values in the five-days-after-ligation group were larger than those in the control group, but not significantly.
    Comparison of the peak T1 values in the one-day-after-ligation group with those of the control group revealed that the T1 value of phosphodiester was significantly larger than that in control group.
    It is suggested that dysfunction of phospholipid metabolism appears in the early phase of hepatic dysfunction due to obstructive jaundice.
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  • Kentaro YOSHIOKA, Shinichi KAKUMU, Akehiko FUJI, Hirobumi TAHARA, Keni ...
    1987 Volume 84 Issue 6 Pages 1251-1257
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    To elucidate the nature of the non-keratin component of Mallory bodies (MBs), monoclonal anti-Mallory body antibody, anti-NMB-3, was prepared using cell fusion technique. Anti-NMB-3 reacted with MBs in the liver but not with intermediate filaments (IFs) of normal human liver or skin. Anti-NMB-3 reacted with MBs of alcoholic hepatitis, Wilson's disease, primary biliary cirrhosis, fatty liver of diabetes mellitus, hepatocellular carcinoma, and griseofulvin-treated mice under immunofluorescence microscopy. In western blotting, anti-NMB-3 reacted with the bands of molecular weight (MW) 42 and 70-92 kd of MBs separated by SDS-polyacrylamide gel electrophoresis, while polyclonal anti-keratin antibody reacted with the bands of MW 45, 55, 62, and 66 kd. These results indicate that anti-NMB-3 reacts with a non-keratin component of MBs. Anti-NMB-3 may be useful for the study of the nature of MBs, and immunofluorescence microscopy with anti-NMB-3 may be a good tool for detecting MBs in liver specimens.
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  • Takuro MIYAMURA, Shigeyuki UCHIDA
    1987 Volume 84 Issue 6 Pages 1258-1264
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The effect of serum lipoprotein fractions on the normal lymphocyte proliferation induced by Con A, PHA and PWM was studied in chronic liver disease patients and in normal subjects.
    Very low density lipoprotein+low density lipoprotein (VLDL+LDL) fraction obtained from chronic liver disease patients showed the suppression of lymphocyte proliferation similar to that of normal subjects. High density lipoprotein (HDL) fraction obtained from chronic liver disease patients showed a significant degree of suppression (p<0.01), whereas HDL fraction from normal subjects did not show suppression. The suppression of HDL fraction in chronic liver disease patients showed a significant correlation with apoprotein E (apo E) concentration (p<0.002), even though there was no difference in apo E concentration between chronic liver disease patients and normal subjects. These results suggest that the suppression was due to abnormal apo E and/or an alteration of the lipid portion of the HDL.
    In conclusion, lipoproteins, especially HDL, play an important role in the modulation of immune response in chronic liver disease.
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  • Hiroshi SUZUKI, Michihiro SUZUKI, Kazuhiko OKABE, Toshiyuki UCHIKOSHI
    1987 Volume 84 Issue 6 Pages 1265-1271
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Serum immunoreactive prolyl hydroxylase (SIRPH) was measured by a new method of sandwich enzyme immunoassay using monoclonal antibody reacting with the beta-subunit of human prolyl hydroxylase. The determination of SIRPH has been evaluated as a useful diagnostic parameter of hepatic fibrogenesis. However, in some cases of liver cirrhosis or hepatocelular carcinoma remarkable elevation of SIRPH was observed. The exact mechanism of the elevation remains to be elucidated more in detail, however, some stimulative mechanism on the induction and secretion of this enzyme might be involved in these diseases besides hepatic fibrogenesis.
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  • Chie KODAMA, Yasuhiro MIZOGUCHI, Machiyo SAKAI, Yohko KONDO, Yoshihide ...
    1987 Volume 84 Issue 6 Pages 1272-1278
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Indoleacetic acid, a metabolite of tryptophan, increases in the blood serum at an early stage in the induction of liver injuries. To investiage whether this indoleacetic acid is derived from hepatocytes, we measured the levels of tryptophan, tryptamine and indoleacetic acid in hepatocytes as well as their culture fluids at various intervals after loading tryptophan on isolated hepatocytes. As a result, we found that the levels of tryptamine and indoleacetic acid in both hepatocytes and their culture supernatants increased significantly in a time-dependent manner. Pargyline, an inhibitor of monoamine exidase which catalyzes the formation of indoleacetic acid from tryptamine, caused an increase in the tryptamine level and a decrease in the indoleacetic acid level in isolated hepatocytes and their culture fluids. These results indicated that there is a metabolic pathway which produces indoleacetic acid from tryptophan in hepatocytes.
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  • Comparison with CT
    Masaharu YOSHIKAWA, Masaaki EBARA, Masao OHTO, Yoshiro WATANABE, Shoui ...
    1987 Volume 84 Issue 6 Pages 1279-1288
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    MRI (Magnetic Resonance Imaging) was performed in 74 patients with chronic hepatitis, liver cirrhosis, idiopathic portal hypertension, Budd-Chiari syndrome, extrahepatic protal vein occlusion, Wilson disease and hemochromatosis. We measured relaxation time of the liver and the spleen in these patients and compared MRI with CT in the diagnostic capability. MRI was superior to plain CT in the detection of collateral vessels in liver cirrhosis and extrahepatic protal vein occlusion. MRI could also demonstrate the occluded part of the inferior vena cava in Budd-Chiari syndroem. However, MRI was almost the same as CT in the visualization of the hepatic configuration in liver cirrhosis. In liver cirrhosis, T1 values of the liver and the spleen were longer than those in normal controls, and T1 values of the liver were correlated with ICG R-15. Hepatic T1 values in Budd-Chiari syndrome were longer than those in normal controls.
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  • With Special Reference to Comparison of Bile with Chenodeoxycholic Acid Treatment and Bile with Ursodeoxycholic Acid Treatment
    Masashi YONEDA, Naoki TAMASAWA, Isao MAKINO, Kazuo TAKEBE, Rikio KOGAW ...
    1987 Volume 84 Issue 6 Pages 1289-1294
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The size of molecular aggregates (generally, micelle) formed by bile acid in human gallbladder bile was studied by utilizing disposal ultrafiltration unit (cut-off molecular weight: 10, 000 and 1, 000).
    In gallbladder bile without any bile acid treatment (n=20), 66% of total bile acid was observed in large aggregate fraction (molecular weight: above 10, 000) and 18% in small particle fraction (dimer and monomer). In bile with chenodeoxycholic acid treatment (n=8) the large fraction was increased to 73% of total bile acid and small fraction was decreased to 13%, as compared with gallbladder bile without any bile acid treatment. On the otherhand, in bile with ursodeoxycholic acid treatment (n=8), large fraction was decreased to 59% and small fraction was increased to 24%. Additionally, it should be noted that major constituent of dimer and monomer was cholic acid or ursodeoxycholic acid. Thus, distribution of size of molecular aggregates in bile with chenodeoxycholic aicd treatment was significantly different from that in bile with ursodeoxycholic acid treatment.
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  • A Study on Mechanism of Polymerization in Black Pigment, and Precipitation of Calcium Carbonate
    Yoshibumi NIITSUMA, Noriyoshi SUZUKI, Fumiaki SHINYA, Hideo ISE
    1987 Volume 84 Issue 6 Pages 1295-1303
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Black stones were analyzed by infrared absorption spectroscopy, and attempts were made at synthesis of black pigment in order to reveal the mechanism of polymerization in black pigment.
    Besides, an experimental effort was made to explain the mechanism of precipitation of calcium carbonate in black stone.
    The spectra of black pigment in black stones were the same as those of synthetic calcium bilirubinate in position of respective absorption bands, and resembled in general aspect.
    The former spectra, however, differed from the latter in the intensity of absorption. From these findings, black pigment was identified as polymers of calcium bilirubinate, and the basic structures of them were identical each other.
    Moreover, the black pigment prepared from synthetic calcium bilirubinate in alkaline solution was almost identical with the black pigment in black stones both in general properties and spectroscopic findings. These facts suggest that calcium bilirubinate preserved in alkaline solution for a long time transformed into a high molecular weight polymer, i.e. black pigment.
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  • A CASE REPORT AND REVIEW OF THE LITERATURE
    Tomoyuki SAMESHIMA, Hidehisa OOI, Hiroshi TOUZINBARA, Shuushi MARUTA, ...
    1987 Volume 84 Issue 6 Pages 1304-1309
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Mitsuro CHIBA, Pablo MORIKAWA, Hiromichi ARAKAWA, Osamu MASAMUNE
    1987 Volume 84 Issue 6 Pages 1310-1313
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Shigeo KATO, Hitoshi ASAKURA, Eiki ICHIKAWA, Shigeru KURAMOCHI, Toshih ...
    1987 Volume 84 Issue 6 Pages 1314-1319
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Shigetaka SUGIHARA, Mitsuharu SATOU, Toshiharu SUGIYAMA, Kouji OKUDA, ...
    1987 Volume 84 Issue 6 Pages 1320-1324
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Satoru TAMURA, Kazuichi OKAZAKI, Masanori MORITA, Hirochika KITAJIMA, ...
    1987 Volume 84 Issue 6 Pages 1325-1330
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Takahiko IGAWA, Makoto OOISHI, Naoyuki IBE, Tosio UENO, Ryouyo TAKEDA, ...
    1987 Volume 84 Issue 6 Pages 1331-1333
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Masashi KODAMA, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1987 Volume 84 Issue 6 Pages 1334
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • ROLE OF LEUKOTRIENES
    Shingo TSUJI, [in Japanese], [in Japanese], [in Japanese]
    1987 Volume 84 Issue 6 Pages 1335
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Akihiro NOGUCHI, [in Japanese], [in Japanese], [in Japanese], [in Japa ...
    1987 Volume 84 Issue 6 Pages 1336
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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