Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 84 , Issue 8
Showing 1-23 articles out of 23 articles from the selected issue
  • Toshiki TAKEMURA, Toshikazu YOSHIKAWA, Kazuhide IWAMOTO, Wataru FUKUDA ...
    1987 Volume 84 Issue 8 Pages 1567-1572
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Endoscopic injection sclerotherapy (EIS) with human thrombin was carried out in twelve patients with esophageal varices induced by liver cirrhosis with or without hepatocellular carcinoma. The changes in blood pressure, coagulation and fibrinolysis, and serum complement systems including anaphylatoxins such as complement 3a (C3a) and complement 5a (C5a) were investigated after EIS. Immediately after EIS, systolic blood pressure was reduced. Platelet counts, plasma fibrinogen level, and serum antithrombin III (AT-III) activities decreased significantly. The serum level of fibrin and fibrinogen degradation product increased significantly. These changes were most remarkable two hours after EIS. There were no significant changes in serum CH50, serum complement 4 (C4) or complement 3 (C3). No bleeding signs or pathophysiological findings usually observed in disseminated intravascular coagulopathy were noted. The serum levels of anaphylatoxins such as C3a and C5a increased significantly at the same time of trnasient hypotension after EIS. These results suggest that anaphylatoxins play a role in transient hypotension after EIS.
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  • Shinpei IMAI, Makoto ITOH, Kohei KATSUMI, Yoshifumi YAKOYAMA, Tadahisa ...
    1987 Volume 84 Issue 8 Pages 1573-1578
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Bilateral submandibular glands were removed in rats fasted overnight and anesthetized with hexobarbital. After surgery, submandibular glands removed rats (SMR rats) and sham operated rats were allowed free access to food and water until experiment. In another SMR rat group, mouse EGF (Collaborative Research Co.) dissolved in drinking water, in addition to food, was administered daily in a dose of 10μg/kg until experiment (SMR+EGF rats). Ten days after surgery, the following studies were performed in these three rat groups.
    study 1: Gastric juice in basal state was collected each 15min for 1hr by the Ghosh-Schild's method in rats fasted overnight and anesthetized with Urethane. Gastric secretion was then maximally stimulated by i.p. administration of 10μg/kg AOC-tetrapeptide and gastric juice was collected by the same procedure as in the basal state. Acid concentration in each gastric juice collected was determined by neutralization with 0.01N NaOH. Both basal (BAO) and maximal acid output (MAO) were then calculated and expressed as μEq/hr.
    study 2: In rats fasted ovenight and anesthtized with ether, the stomach was removed and fixed in formalin. Two strips of tissue were removed from the corpus. Histologic tissue sections were then prepared and stained with PAS-alcianblue. The area of the presence of PAS-stained intramucosal mucus was evaluated on the histologic specimens using a video image processor (Olympus Co. VIP-21 CH) and expressed as the percent of the total area of the mucosa.
    study 3: One ml of 0.6N HCl was administered i.g. to rats fasted overnight. One hour later, rats were killed and the total length of gastric mucosal lesions was measured and expressed as ulcer index (mm).
    In SMR rats, BAO, PAS-stained intramucosal mucus and ulcer index were 20.4±3.5μEq/h, 2.5± 0.3% and 85±12mm, respectively. These values were significantly different from those in sham operated rats (BAO, 7.7±0.9μEq/h, mucus, 7.9±0.9%, ulcer index, 25±4mm). No significant differences were observed in MAO among three rat groups. In SMR+EGF rats, BAO was considerably reduced to 11.6±2.6μEq/h although the values were not statistically significant from those in SMR rats. The decrease of PAS-stained mucus was inhibited in SMR+EGF rats with the percent scores, 4.3±0.2%, being significantly greater than those in SMR rats. Gastric lesions induced by HCl in SMR +EGF rats were markedly inhibited almost to the same extent as in sham operated rats. The ulcer index, 44±8mm, was significantly smaller than that in SMR rats.
    We conclude that endogenous EGF derived from the submadibular glands plays an improtant role in the protection against HCl-induced mucosal lesion formation by reducing acid secretion and maintaining intramucosal mucus in the rat stomach.
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  • Motoyasu KUSANO, Toshikazu SEKIGUCHI, Toshio NISHIOKA
    1987 Volume 84 Issue 8 Pages 1579-1590
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Plasma motilin concentration, gastroduodenal motility and pH were investigated simultaneously in eight normal subjects (N) and fourteen patients with duodenal ulcer (DU).
    The following results were obtained; (1) In N group, plasma motilin increased gradually in phase II correlated with gastroduodenal motility, its peak was associated with the onset of gastric IMC and decreased already in duodenal phase III. (2) In DU group, the incidence of IMC was significantly lower than N, and plasma motilin was not correlated with gastric motility. In several DU patients, motilin peak was appeared without IMC.
    It was suggested that the increase of plasma motilin was required in order to initiate gastric IMC, but was not the final mediator. The authors concluded that gastroduodenal motor dysfunction in DU was caused by some malajustment mechanisms of gastroduodenal motility except for motilin.
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  • Kazuyo AMANO
    1987 Volume 84 Issue 8 Pages 1591-1604
    Published: 1987
    Released: December 26, 2007
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    The relationship between intestinal microflora and steatorrhoea was studied in patients with extensive gastric resection for peptic ulcer, by microbiologically analyzing intestinal contents taken from upper small intestine by use of the intestinal capsules swallowed with test meals.
    Steatorrhoea was observed in patients whose upper small intestinal contents contained more than 108/ml of bacteria. Particularly when the number of total obligate anaerobes exceeded 108/ml, the incidence of steatorrhoea was increased.
    The relationship between the bacterial counts of each bacterial group in the small intestinal contents and the percentage of faecal fat excretion against dietary intake of fat was also examined. Positive correlations were found in Bacteroidaceae, Eubacteria and Streptococci. Particularly in Bacteroidaceae, the highest values were found in detection rates, bacterial counts and a correlation coefficient in the examined patietns. The above-mentioned bacteria contained strains capable of deconjugation of conjugated bile acids.
    These results indicated that steatorrhoea was induced by disturbance of micellar formation of lipids in association with overgrowth of the bacteria capable of deconjugation of conjugated bile acids in the upper small intestine.
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  • Sumio FUJINUMA
    1987 Volume 84 Issue 8 Pages 1605-1615
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Fatty acid binding protein (FABP) were studied immunohistochemically in 12 patients with Crohn's disease, 11 patients with intestinal tuberculosis, and 28 control cases in order to clarify the disturbance of absorption and metabolism of fat in Crohn's disease. The patients of Crohn's disease consisted of 6 with intravenous hyperalimentation (IVH) and 6 without IVH. Terminal ileum of surgically resected specimens provided the material for this study. In nonulcerated mucosa, the number of FABP positive cells and the incidence of FABP positive cells in villi showed no significant difference between control cases, intestinal tuberculosis, and Crohn's disease without IVH. But they showed lowering in Crohn's disease with IVH. At the ulcer margin, they revealed no difference between intestinal tuberculosis and Crohn's disease with or without IVH.
    These results imply the evidence that fatty acid binding protein in ileal absorptive cells exist enough to participate in cellular fatty acid transport and metabolism in Crohn's disease without IVH. Moreover, the decrease of incidence of FABP positive cells in Crohn's disease with IVH might be caused by cessation of fat intake in gut.
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  • Masanori MORITA, Kazuichi OKAZAKI, Yasuro YAMAMOTO
    1987 Volume 84 Issue 8 Pages 1616-1622
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    A new gastrointestinal antigen was obtained using the monoclonal antibody (MPB11) which was produced by hybridoma cells made by fusing mouse myeloma cells (P3U-1) with the spleen cells of a BALB/c mouse that had been immunized with suspended colonic cells in an F344 rat. Using the peroxidase labeled, antibody-staining method, the presence of the antigen against MPB11 was demonstrated in the surface mucin and crypt of the stomach, the small intestine, and the colon in the rat. It was not detected in the other organs i.e. esophagus, liver, kidneys, heart, lungs and spleen. In human tissues the antigen against MPB11 was demonstrated in the mucin in the stomach and duodenum, but colonic goblet cells did not have the antigen.
    It's molecular weight was estimated at 60 kilodaltons by polyacrylamide gel electrophoresis and Western immunoblotting.
    The presence of sialoprotein in the antigenic determinant was confirmed by examination with the enzymes (exoglycosidase, neuraminidase and pronase).
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  • Jugoro TAKEUCHI, Masahiko OKUDAIRA, Akira TAKADA, Yasuyuki OHTA, Tadas ...
    1987 Volume 84 Issue 8 Pages 1623-1630
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    By the national surveillance study performed in 1978, we found a significant increase in the incidence of alcoholic liver disease between 1968 and 1977 (Jpn J Gastroenterol 76: 2178, 1979).
    In April 1986, we have done a subsequent surveillance study of nationwide 113 hospitals to see its recent, yearly incidence between 1976 and 1985. Of the total of 105482 patients with various liver diseases who admitted in the hospitals during the study period, 14917 were diagnosed as alcoholic liver disease (14254 male patients and 663 female): The yearly percentage of the patients with alcoholic liver disease was analyzed among those with various liver diseases and found to be nearly the same (about 14%) in each observation year. However, the yearly incidences of both alcoholic hepatitis and alcoholic cirrhosis increased significantly; the incidence of alcoholic hepatitis in alcoholic liver disease moved from 15.3% in 1976 to 17.4% in 1985, and alcoholic cirrhosis from 37.1% to 43.7%. In addition, in the patients with alcoholic hepatitis, it was found a significant increase in the percentage of alcoholic patients with severe, fulminant hepatitis (from 6.4 to 7.4%) during the observation period.
    The proportion of alcoholic cirrhosis to total cirrhosis was 19.1%, and its yearly incidence showed little change during the study period. On the other hand, an increse in the occurrence rate of hepatocellular carcinoma was found in patients with alcoholic cirrhosis, and it reached at 25.5% in 1985.
    The present surveillance study revealed that the increasing rate of the incidence of alcoholic liver disease is attenuating since the end of 1970th, whereas recently, a severe type of the disease becomes to be not very rare; indeed, it is increasing.
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  • Yoshiro MATSUDA, Minoru YASUHARA, Akira TAKADA
    1987 Volume 84 Issue 8 Pages 1631-1638
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The serum levels of immunoreactive prolyl hydroxylase β-subunit (IRβ-PH) and procollagen type III N-terminal aminopeptide (Pro-III-N-P), which have been generally considered to be markers of hepatic fibrogenesis, were measured in acute hepatitis (AH) in order to clarify the differences between the markers for the evaluation of hepatic fibrogenesis. The mean values of both serum marker levels of AH were significantly higher than those of the normal controls. Serum Pro-III-N-P levels were abnormally high in almost all cases of AH. Only half of the AH cases, however, showed abnormally high serum IRβ-PH levels. In almost all cases of AH, serum IRβ-PH levels decreased to nearly normallevels within 3 weeks of admission and retained normal values throughout the healing course. However, serum Pro-III-N-P levels remained abnormally high in many AH cases. These results indicated that serum IRβ-PH levels were more specific than serum Pro-III-N-P for the evaluation of collagen synthesis in the liver.
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  • Yoshiaki INUI, Sumio KAWATA, Takashi KOBATAKE, Shinji TAMURA, Shigenor ...
    1987 Volume 84 Issue 8 Pages 1639-1644
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The livers of Zucker obese (fa/fa) and lean (fa/-) rats treated with the α-glucosidase inhibitor, AO-128, for 10 weeks were biochemically and morphologically investigated. Body weight (p<0.01), triglyceride in the liver (p<0.05) and free fatty acids in portal blood (p<0.01) were decreasd in the Zucker obese rats. In addition, lipid droplets in heaptocytes were shown to be decreased markedly in the Zucker obese rats. Thus, we concluded that reduced transport of free fatty acids into hepatocytes via portal blood might be a major responsible for the decrease in lipid concentrations observed in portal blood and the liver of the Zucker obese rats.
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  • The Effects of Indomethacin on Tumor Growth
    Seiichiro ANDOH, Takahiko NOHARA, Akira NAKASE
    1987 Volume 84 Issue 8 Pages 1645-1654
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Effects of indomethacin on human hepatocellular carcinoma were studied in nude mice and in cell cultures. Transplanted tumors in nude mice well maintained the morphological appearance and AFP producing ability of the original tumor. Indomethacin was given intraperitoneally (4mg/kg B.W.). In three of 6 mice which were given indomethacin every day starting on the day of tumor inoculation, tumor reduced their sizes after periods of growth for approximately 2 weeks. In the other 3 mice, necrotic areas were increased, though tumors continued to increase their sizes. Microangiograms showed that, in this group, tumors were hypovascular and those blood vessels were small and fine. Histology revealed that the sinusoidal-network-composing endothelial cells were immature.
    In contrast, indomethacin added to culture medium (10-10M, 10-8M and 10-6M) stimulated cell proliferation in cell cultures of human hepatocellular carcinoma.
    These findings suggest a possibility that indomethacin has influence on tumor growth through inhibition of tumor neovascularization.
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  • Toshihiko KAWASAKI, Fuminori MORIYASU, Osamu NISHIDA, Nobuyuki BAN, Ta ...
    1987 Volume 84 Issue 8 Pages 1655-1660
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Blood flow directions of the portal trunk, splenic vein, and superior mesenteric vein were studied using an ultrasonic Doppler duplex system in 146 healthy adults, 133 patients with liver cirrhosis, 76 with hepatocellular carcinoma, 31 with idiopathic portal hypertension, 134 with chronic hepatitis, 18 with acute hepatitis and 142 with other diseases.
    Spontaneous hepatofugal flows in one or more vessels of the portal system were detected in 14 patients, and post-operative hepatofugal flows in the portal system were detected in 19 patients.
    Spontaneous hepatofugal flows in the portal trunk were detected in two patients with liver cirrhosis. In one of these two patients, the portal blood flow became to-and-fro after 2 months' medication, and in the other the flow became hepatopetal after 7 days' medication. Spontaneous hepatofugal flows in the portal trunk were much rarer than post-operative hepatofugal flows in the portal trunk, and this suggests there is some functional difference between spontaneous shunts and operative shunts.
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  • Intestinal Metaplasia detected in the Gallabadder Carcinoma
    Jôji ISEKI
    1987 Volume 84 Issue 8 Pages 1661-1667
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The presence of intestinal epithelial metaplasia and endocrine cells was investigated in the mucosa of gallbladder carcinoma. The material consisted of 38 gallbladder carcinomas, all confined to the wall of gallbladder. Areas of intestinal metaplasia within the tumor were found in 25 cases, and those in the mucosa surrounding the tumor were also found in 25 cases. Somatostatin-immunoreactive cells within the tumor were detected in 6 cases, and those outside the tumor in 8 cases. Gastrin-immunoreactive cells within the tumor were detected in none, and those outside the tumor in 5 cases. It seems difficult t oclarify the significance of intestinal metaplasia for the evolution of gallbladder carcinoma from analysis merely of the incidence of intestinal metaplastic changes within or outside the cancerous foci.
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  • Toshihiko SUZUKI, [in Japanese], [in Japanese], [in Japanese]
    1987 Volume 84 Issue 8 Pages 1668-1674
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    As toxic substances injure vital organs, the mortality rate of acute severe pancreatitis is still high. Many kinds of activated enzymes in the blood stream produced by inflammated pancreas may damage the vital organs. Recently, blood exchange transfusion has been used as a therapeutic procedure to remove toxic substances. To assess the therapeutic efficency of blood exchange transfusion, we investigated the survival rate and histological changes in the rats with taurocholate induced severe pancreatitis. Blood exchange transfusion significantly prolonged mean length of survival rate. Early exchange before vital organs were damaged, was more beneficial than late one. Blood exchange transfusion reduced pancreatic edema and pulmonary edama, and improved arterial oxygen pressure.
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  • Report of a case and study of growth factor receptor in colonic adenoma
    Yoko WIMA, Masae KAWAMURA, Mutsuo SHIGEMOTO, Shyuichi SATO, Teiko NAKA ...
    1987 Volume 84 Issue 8 Pages 1675-1678
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Chie TANIOKA, Ken HARUMA, Koji SUMII, Kenji TOKUMO, Hideki MATSUBARA, ...
    1987 Volume 84 Issue 8 Pages 1679-1683
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Bunzo MATSUURA, Kohichi AKAMATSU, Kohichiro KITAI, Hiroshi KIMURA, Yas ...
    1987 Volume 84 Issue 8 Pages 1684-1689
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hiroshi HASEGAWA, Tadahiro TAKADA, Hideki YASUDA, Katsuhiro UCHIYAMA, ...
    1987 Volume 84 Issue 8 Pages 1690-1694
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Morimitsu KAWAI, Takahiko IGAWA, Hiroaki MURAMOTO, Katsuo HARUKI, Sei ...
    1987 Volume 84 Issue 8 Pages 1695-1698
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Shinichi OTA, [in Japanese], [in Japanese], [in Japanese], [in Japanes ...
    1987 Volume 84 Issue 8 Pages 1699
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Takeo IWAMA, [in Japanese], [in Japanese], [in Japanese], [in Japanese ...
    1987 Volume 84 Issue 8 Pages 1700
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yoshinobu FUSE, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1987 Volume 84 Issue 8 Pages 1701
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yoshihide SAKAGAMI, [in Japanese], [in Japanese], [in Japanese], [in J ...
    1987 Volume 84 Issue 8 Pages 1702
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Tetsuya HIRANO, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
    1987 Volume 84 Issue 8 Pages 1703
    Published: 1987
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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