-
[in Japanese]
1987 Volume 84 Issue 9 Pages
1735-1736
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Tsugio EBIHARA, Shohei KOYAMA, Toshiaki OSUGA
1987 Volume 84 Issue 9 Pages
1737-1739
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
Immunohistochemical reactivity of antibody against a synthetic peptide corresponding to positions 160-179 of the Ha-
ras gene product was studied in 67 (78 lesions) surgically resected stomach cancers of humans. The cytoplasma of cancer cells in 31 of 78 lesions (39.7%) was positively stained. Staining positivity of advanced type carcinoma was accentuated than that of early type carcinoma. However, there was no correlation between depth of gastric carcinoma invasion and
ras p21 expression. Immunoperoxidase staining pattern of these tumors was shown by the strong expression of
ras p21 in more superficial glands
versus the lesser amount of
ras p21 expression in deeply invasive glands. Cancer cells of well differentiated type showed to react more frequently to antibody. In nontumorous gastric mucosa, some portions of regenerating epithelial cells and intestinal metaplasia were stained with antibody.
View full abstract
-
Kiyotaka YABUKI
1987 Volume 84 Issue 9 Pages
1740-1748
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
To clarify the role of the sympathoadrenal system in gastric acid secretion, the author evaluated the effects of epinephrine on gastric acid secretion during stimulation with tetragastrin, betazole hydrochloride, bethanechol, and bombesion in dogs with a total gastric fistula. Epinephrine administration reduced gastric acid secretion dose-dependently during stimulation with these agents except betazole hydrochloride. Blood flow in the gastric mucosa did not show direct effects on inhibition of secretion induced by epinephrine. The serum gastrin value markedly increased, but gastric acid secretion decreased following epinephrine administration (2.0μg/kg/min) during stimulation with bombesin. These results suggest that epinephrine secreted from the adrenal medulla directly acts on parietal cells, inhibiting gastric acid secretion.
View full abstract
-
Tamotsu SUKO
1987 Volume 84 Issue 9 Pages
1749-1754
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
PPD and PHA skin tests were carried out on 73 patients with hepatocellular carcinoma (HCC) for the index of cellular immunity at the time of diagnosis established. Studies were mode on the relationship between clinical data and these tests. Clinical data included space occupying rate of HCC, survival time, symptoms and laboratory evaluations. PPD skin test showed an increased negativity along with increasing of tumor size. The positive PPD test had a tendency of positive correlation to the survival time and body mass index, however, inverse correlation with log (AFP), GOT and LDH was obserbed.
The survival rate in group of positive PPD tests more than 20mm skin reaction was significantly greater than that in those less than 20mm (p<0.001).
In patients with hypoalbuminemia, PPD and PHA tests showed lower reaction compared to the patients with normoalbuminemia.
Consequently it is suggested that PPD skin test is useful for prediction of prognosis in the patients with HCC.
View full abstract
-
Hitoshi NAKANO
1987 Volume 84 Issue 9 Pages
1755-1763
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
Remarkable elevation of total serum immunoglobulin M (IgM) is a characteristic feature of acute hepatitis A. The cause of this elevation was investigated and the results were obtained as follows; (1) In examination of fecal microflora in patients with acute hepatitis, no significant difference was found between type A and B hepatitis. (2) In patients with acute hepatitis A, serum level of IgM class anti-lipid A antibody activity during acute pahse was significantly higher than that of acute hepatitis B, however, it decreased uniformly during convalescent phase in all cases of hepatitis A. This change was most remarkable in a hepatitis A patient with endotoxemia. The change of serum level of IgM class anti-lipid A antibody activity was parallel with change of total serum IgM.
These results suggest that the reticuloendotherial system (RES) of the liver is markedly damaged in acute hepatitis A. If IgM antibody is producted against many gut derived bacterial antigens spilled over to the peripheral blood because of damaged liver RES, the elevation of total serum IgM in acute hepatitis A could be mostly understood.
View full abstract
-
Norio HORIIKE, Morikazu ONJI, Yasushi OGAWA, Kojiro MICHITAKA, Toshiko ...
1987 Volume 84 Issue 9 Pages
1764-1771
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
The change in HBV markers of 118 patients with chronic hepatitis type B were studied with special reference to their clinical courses. The patients were divided into 3 groups (HBeAg positive group: 85 cases. anti-HBe positive and abnormal GPT group: 16 cases. anti-HBe positive and normal GPT group: 17 cases)
Serum HBV-DNA was detected in 99%, 91%, 0%, respectively. Likewise HBV-DNA-P was in 95%, 94%, 0%, and HBcAg in livers was detected in 51%, 20%, 0%, respectively. An increase of HBV-DNA and DNA-P preceeding exacerbation of hepatitis was detected in 94% of the HBeAg positive group and in 82% of the anti-HBe positive and abnormal GPT group.
In 5 cases of the anti-HBe positive and abnormal GPT group, GPT was normalized in the successive peroid. However, in continuous abnormal GPT cases, 3 of 4 progressed to liver cirrhosis and 2 cases resulted in hepatic failure. These data suggest that patients with anti-HBe positive chronic hepatitis showing GPT fluctuation were related to HBV replication and that these cases should be observed carefuly.
View full abstract
-
Hiroaki WATANABE
1987 Volume 84 Issue 9 Pages
1772-1777
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
Urinary neopterin excretion levels were determined in 7 esophageal, 36 gastric, 10 colonic, 8 pancreatic and 18 hepatocellular carcinomas, and in 20 normal controls.
Urinary neopterin excretion increased significantly in gastric, colonic, pancreatic and hepatocellular carcinoma more than normal controls.
Percent abnormality of urinary neopterin in gastric cancer was 42% and urinary excretion increased in proportion as the stage advanced.
Urinary neopterin excretion in seven patients with gastric cancer decreased significantly after gastrectomy.
Urinary neopterin excretion level in patients with hepatocellular carcinoma was correlated positively with their size.
From these results, it may be suggested that urinary neopterin excretion level reflects both the clinical status of the patient and tumor size, and is useful for observing clinical course and judging effects of the treatment.
View full abstract
-
Yoshinobu SHIODA, Akira TOKUNAGA, Noritake TANAKA, Masahiko ONDA, Goro ...
1987 Volume 84 Issue 9 Pages
1778-1786
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
The distribution of Ulex europaeus agglutinin-1 (UEA
1) binding sites was investigated in normal and 1, 2-dimethylhydrazine (DMH) treated F344 rat bowel.
In normal rat small intestine, UEA
1 binding was distributed in the mucus of goblet cells, on the granules of Paneth cells and along the microvillous surface of epithelial cells of both crypt and villi. A faint staining was also detected along the latero-basal membrane.
The staining intensity of the lectin was rather greater in the ileum than in the jejunum. In the normal proximal colon, UEA
1 binding was observed in the mucus of columnar epithelial cells in the half lower crypt, whereas little or no staining was seen in normal distal colon.
In adenoma and cancer induced by DMH, UEA
1 was frequently and intensely positive along the apical surface of the tumors, and sometimes it also was bound to cytoplasmic site.
In the distal colon of DMH treated animals, UEA positivity of biopsies from normal appearing mucosa increased progressively with time, although UEA
1 was seldom observed at the begining of the experiment. At least one or two biopsies containing increased amount of UEA
1 binding sites were obtained from every animal before appearance of tumor. Thus the UEA
1 binding appeared to be a useful marker of the neoplasms and the high risk mucosa for cancer in DMH treated F344 rat distal colon.
View full abstract
-
Significance of Intestinal Metaplasia, Adenoma, and Dysplasia
Joji ISEKI
1987 Volume 84 Issue 9 Pages
1787-1796
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
Thity-eight gallbladder carcinomas were classified into four types on the basis of gross appearances, that is, type A: pedunculated polyp type, type B: sessile polypoid type, type C: superficially spreading type, type D: diffuse infiltrating type.
The significance of intestinal metaplasia, adenoma, and dysplasia for the evolution of gallbladder carcinoma was investiaged in each type. As a result, three modes of histogenesis of gallbladder carcinomas were suggested. In the first mode, carcinomas showing the gross appearence of type A, arise from areas of adenoma, where intestinal metaplastic changes are unusually observed. In the second mode, islands of intestinal metaplasia, or dysplastic areas accompanied with intestinal metaplasia make a considerable role in the histogenesis of carcinomas of type B and C. In the third mode, dysplastic areas which originate in the mucosa with severe chronic cholecystitis may contribute to the inception of carcinomas of type D.
View full abstract
-
Hisami ANDO, Takahiro ITO, Tetuyuki SUGITO
1987 Volume 84 Issue 9 Pages
1797-1801
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
Most literature states that choledochal cysts lack muscles and mucosa and dose not mention the presence of a muscles in the wall of the lower portion of the choledochal cyst and in the narrow portion which lies in the pancreatic tissue. The present studies were made in order to determine whether or not smooth muscle may exist as a structural component of the wall.
Specimens were obtained at laparotomy from 9 patients with choledochal cyst. These specimens were examined microscopically and the thickness of the smooth muscle bundles and mucosal layer was measured with a micrometer.
Numerous smooth muscle bundles were found in the lower part of the choledochal cyst wall. Longitudinal and circular muscles formed a layer around the wall of the duct. Longitudinal muscle bundles had 3 to 8 layers and were 10 to 50μ thick. Circular muscle bundles had 1 to 9 layers and were 60 to 250μ thick. Muscle layers were 0.7 to 1.6mm in thickness and were localized 6 to 25mm in length. In 7 patients, the lower protion of the choledochal cyst wall exibited tall columnar epithelium 0.2 to 0.7mm in height and 2 to 20mm in length. On the other hand, there was no distinct muscle layer and short columnar epithelium was present in the narrow portion.
View full abstract
-
Satoru TAMURA, Kazuichi OKAZAKI, Yasuro YAMAMOTO
1987 Volume 84 Issue 9 Pages
1802-1812
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
A new pancreatic ductal cell antigen was identified by using a monoclonal antibody SP3-1 which was produced by hybridoma cells made by fusing mouse myeloma cells (P3U-1) with spleen cells of a BALB/c mouse immunized with pig pancreatic antigen. The homogenized pig pancreas was fractionated by gel filtration through Sephadex G-100 and the void fraction was absorbed with DEAE-Sephacel and then the 3rd eluted fraction was used for immunogen. Immunohistochemically, using the peroxidase labeled antibody-staining method, the antigen against SP3-1 was demonstrated in the pancreatic ductal cell, bile ductal cell, ductal cells of the salivary gland and the esophageal gland, and the renal tubules of the pig and human. The molecular weight of the recognized antigen was estimated at 60 kilo-daltons by polyacrylamide gel electrophoresis and Western immunoblotting. The varous enzyme treatments suggested that the antigenic determinant contained protein but not sialic acid or glucose, and that isotype of SP3-1 was IgM.
View full abstract
-
Hirotake HISANO, Seiki MATSUNO, Masao KOBARI, Toshio SATO
1987 Volume 84 Issue 9 Pages
1813-1820
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
Sensitivities to anti-tumor drugs, Mytomycin C (MMC), Aclarubicin hydrochloride (ACR), Doxorubicin hydrochloride (ADR), Cisplatin and 5-fluorouracil (5FU), were examined using PK-1, PK-8, PK-9, PK-12, PK-14 and PK-16 cell lines derived from human pancreatic cancer. These cell lines showed different sensitivities to each of these anti-tumor drugs. The concentrations required for 50% growth-inhibition (IC
50) after 2 hours of exposure were 0.096-0.35μg/ml for MMC, 0.0074-0.0076μg/ml for ACR, 0.033-0.23μg/ml for ADR, 0.35-1.9μg/ml for Cisplatin and 21-42μg/ml for 5FU. The IC
50 of each anti-tumor drug decreased significantly after 48 hours of exposure. The combination of two out of three drugs, MMC, ACR and 5FU, resulted in a synergistic effect to inhibit the proliferation of pancreatic cancer cells.
View full abstract
-
T. FURUKAWA, Y. MATSUOKA, H. TAKIZAWA, T. SAITO, H. KISHIMOTO, J. FUKU ...
1987 Volume 84 Issue 9 Pages
1821-1824
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Tatsuya KUBO, Kenichi NISHIDA, Kouichi FUJITA, Nobuo OKABE, Kazuhiko T ...
1987 Volume 84 Issue 9 Pages
1825-1829
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Tsunehisa KAWASAKI, Yoshihiro FUKUDA, Yukitoki IKEHARA, Hiroshi NAKAYA ...
1987 Volume 84 Issue 9 Pages
1830-1834
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Yasushi KASAI, Masahiro SUENAGA, Akio HARADA, Toshiaki NONAMI, Akimasa ...
1987 Volume 84 Issue 9 Pages
1835-1838
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Masakazu EGAWA, Yoshinori MORIMOTO, Hidenori NAKATA, Takeshi HARA, Kik ...
1987 Volume 84 Issue 9 Pages
1839-1843
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Hidetomo NAKAMOTO, Yasutaka INAGAKI, Takeyuki MARUYAMA, Takashi MASUDA ...
1987 Volume 84 Issue 9 Pages
1844-1849
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
A CASE THAT WAS DIFFICULT TO DIAGNOSE THE ORIGINAL LESION
Seiichiro ANDOH, Seiji YANG, Masayuki ITAKURA, Seikon KIN, Junichi ONO ...
1987 Volume 84 Issue 9 Pages
1850-1854
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Toshiya KOJIMA, Tohko SHIROTA, Nobuyuki KAKEI, [in Japanese], Kensuke ...
1987 Volume 84 Issue 9 Pages
1855-1858
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Tatsuo JIBU, Hideo NAGAI, Daisuke SENBA, Yoshiyuki WADA, Akira KURODA, ...
1987 Volume 84 Issue 9 Pages
1859-1862
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Takashi KAWAI, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1987 Volume 84 Issue 9 Pages
1863
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Kuniyasu MATSUI, [in Japanese], [in Japanese], [in Japanese], [in Japa ...
1987 Volume 84 Issue 9 Pages
1864
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Kazuhiro KATAYAMA, [in Japanese], [in Japanese], [in Japanese], [in Ja ...
1987 Volume 84 Issue 9 Pages
1865
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS
-
Susumu SHIOMI, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1987 Volume 84 Issue 9 Pages
1866
Published: 1987
Released on J-STAGE: December 26, 2007
JOURNAL
FREE ACCESS