Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 86 , Issue 5
Showing 1-21 articles out of 21 articles from the selected issue
  • Masaki YOSHIDA, Ichiro IMOTO, Shiro SUZUKI
    1989 Volume 86 Issue 5 Pages 1025-1030
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We investiaged the distribution and prevalence of Campylobacter pylori in the stomach and duodenum. In this study, 500 biopsy specimens were obtained from 245 patients. In each case, biopsy specimens were taken from more than 2 sites. C. pylori was detected by culture, urease test and acridine-orange stain. C. pylori was not detected on the intestinal metaplasia, gastric cancer tissue and duodenal mucosa without gastric metaplasia. In 21% of cases, C. pylori was detected in only one site. Because of the patchy distribution of C. pylori, more than 2 biopsy specimens from different sites were needed to avoid sampling error. Detection rate of C. pylori was almost equal in antrum, angle and body as well as in male and female.
    H2 receptor antagonists did not affect the detection rate of C. pylori. According to the endoscopic diagnosis of the biopsied site, C.pylori was detected in 87% of gastric ulcer, 60% of duodenal ulcer (duodenal mucosa with gastric metaplasia), 73% of chronic gastritis and 62% of endoscopically normal gastric mucosa.
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  • Tetsuo ARAKAWA, Takashi FUKUDA, Atsushi NAKAMURA, Hiroshi SATOH, [in J ...
    1989 Volume 86 Issue 5 Pages 1031-1036
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Damage caused by ethanol and its prevention by 16, 16-dimethyl PGE2 were tested in fractions rich in surface epithelial cells (F1), rich in chief cells (F2), and rich in parietal cells (F3) isolated from rat stomach in vitro. Ethanol caused cell damage in F1 in a dose-related way and the concentration of ethanol for induction of 50% cell damage was 14.93%. There was no difference in cell damage caused by 15% ethanol between the three different cell fractions. 16, 16-Dimethyl PGE2 (10-6M) significantly inhibited the damage caused by 15% ethanol in all the three fractions. These results suggest that 16, 16-dimethyl PGE2 has direct protective effect on gastric cells and this action is not limited to the specific cell type.
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  • Fumiaki KOIZUMI, Takeshi KAWAMURA, Akira ISHIMORI
    1989 Volume 86 Issue 5 Pages 1037-1043
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Correlation between gastric emptying time and both plasma gastrin and pancreatic polypeptide (PP) levels after meal was studied in 9 normal and 5 streptozotocin (STZ) diabetic dogs. Moreover, the effects of exogenous insulin at doses of 0.2 and 0.4U/kg on gastric emptying and plasma hormone levels were also studied in STZ diabetic dogs. The time required to reach the peak of plasma acetaminophen level, which is the indirect indicator of gastric emptying rate, was significantlty delayed in STZ diabetic dogs than in control dogs. Plasma gastrin response was delayed slightly in STZ diabetic dogs, but the plasma levels were not significantly different between the two groups. Plasma PP levels both in the basal and after postprandial state were significantly higher in STZ dogs than in normal dogs. Infusion of insulin did not affect plasma levels of acetaminophen and gastrin, while it produced a dose-dependent suppression of postprandial PP rise in STZ diabetic dogs. The foregoing data indicate that high level of plasma PP observed in STZ dogs did not correlate with gastric emptying but with insulin deficiency.
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  • Hisao TAJIRI, Daizo SAITO, Shigeaki YOSHIDA, Hajime YAMAGUCHI, Yanao O ...
    1989 Volume 86 Issue 5 Pages 1044-1050
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    A comparative study of local interstitial hyperthermia induced by low power Nd:YAG laser (laser hyperthermia) and microwave hyperthermia was carried out in vivo and in vitro in an attempt to clarify the therapeutic effect and the mechanism. Both therapies, after seven days, caused marked amounts of coagulated necroses in the transplanted tumors of human pancreatic carcinoma. Immeditely after those therapies, electron microscopic examination of the tumor tissues showed membranous, cytoplasmic, nuclear and mitochondrial damage; however, this damage was much more extensive in laser hyperthermia compared to microwave hyperthermia. The cytocidal effects of heating alone, laser hyperthermia and microwave hyperthermia were also studied in vitro. Of the three, laser hyperthermia demonstrated the most notable decrease in viability. Thus, in the mechanism of laser hyperthermia, it seems that the immediate effect is cellular damage caused by both heat energy and Nd:YAG laser light followed by subsequent damage of tumor vessels.
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  • Kunihiko KOYAMA, Akira AOIKE, Tomohide HOSOKAWA, Keiya NAKAMURA, Kiich ...
    1989 Volume 86 Issue 5 Pages 1051-1057
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Immune functions generally decline with aging. However, the onset and the rate of the functional decline may be different in each lymphoid compartments. We studied the effects of aging on the murine Peyer's patch cells, which is a part of gut-associated lymphoid tissue (GALT). Peyer's patch cells of 21-months old aged mice retained a greater capacity to proliferate in the response to mitogens, and non-specific immunoglobuline synthesis in vitro. But Peyer's patch cells of 29-months old aged mice showed a distinct functional defect. So, it is suggested that Peyer's patch cells maintain immune functions untill cetain point of aging and rapidly lose their functions. It is reasonable that GALT maintains the immune functions until certain point of aging, because GALT is one of the first line of defence mechanism and always contact with many dietary and bacterial antigens.
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  • Yumiko TAGUCHI
    1989 Volume 86 Issue 5 Pages 1058-1069
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Sixteen surgical resected cases of "enterocolitis unclassified" diagnosed on the first pathological examination were re-examined. Their incidence was 3.0% of non-neoplastic bowel diseases registered in our department.
    On the re-examination including additional microscopic sections, histological reconstruction, special stainings and precise clinical data of each lesion, the 16 lesions were macroscopically divided to nine types and re-diagnosed as follows; (1) definite diseases: ulcerative colitis and phlegmonous enterocolitis, (2) probable diseases: intestinal tuberculosis and infective enterocolitis, (3) possible diseases: healed ischemic colitis, healed ischemic enteritis, healed infective colitis, and healed Crohn's disease or healed ulcerative colitis, (4) enterocolitis unclassified in a narrow sense.
    In conclusion, patho-morphologically the 16 lesions of enterocolitis unclassified might be sub-divided into 13 lesions with the similarity to the already known inflammatory bowel diseases and hree lesions with enterocolitis unclassified in a narrow sense.
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  • Kazuhiro TASHIRO, Masao KASAHARA, Katsuhiko KAMEI, Yoko TERASHIMA
    1989 Volume 86 Issue 5 Pages 1070-1078
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    This study was performed to elucidate immunohistochemical localization of OAT in the gut mucosa and liver of rats using the ABC method. In the normal rat gut mucosa, strong immunoreactivity of OAT as fine granules was preasent in the surface epithelial cells and apical portion of intestinal glands. In the liver, this reaction was observed in the range of zone 2 to 3 of the pericentral vein and periportal vein. The changes in the intestinal glands of rats with a hyperprotein intake were observed to peak at 2 weeks, but in the liver a strongly positive diffuse reaction thruoghout whole lobules was detected at 4 weeks. This immunoreactive morphological localization of OAT suggests the location of absorption of amino acids in the samll intestine and the distribution of the urea cycle enzyme OAT in the liver. The relationship between absorption of amino acids in the small intestine and the distributionof OAT in the liver was assumed to facilitate feedback repression. The changes in OAT in the mitochondria showed many diverse patterns by immunochenical electron microscopy, and these reactions are presumed to facilitate various types of metabolism in a single cells.
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  • Hitoshi OKADA
    1989 Volume 86 Issue 5 Pages 1079-1088
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Prolyl hydroxylase (PH) is an enzyme acting in early stage of collagen synthesis. We have emphasized the significance of the measurement of serum PH (SIRPH) in relation to liver fibrosis mainly in patients with alcoholic liver disease (ALD). In this study, we determined the localization and positivity of PH by tissue PH stain method (Avidin Biotin Complex method) to clarify the differences in fibrosis between ALD (25 cases) and non-alcoholic liver diseases (non-ALD, 47 cases). Tissue PH was found to be positive in liver cells around Glisson's sheath in early stage of fibrosis, and then in liver cells left within septa and also in mesenchymal cells in the sinusoidal wall as fibrosis progressed.
    Although there were basically no marked differences between ALD and non-ALD, ALD tended to show stronger tissue PH positivity for a degree of fibrosis, PH positivity in parenchymal cells was especially remarkable around pericellular fibrosis in ALD. These results clearly reflected the important role of liver parenchymal cells in progression of fibrosis.
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  • Masao ARAI, Fumio OKUNO, Hiromasa ISHII, Yoshiaki HIRANO, Kazufumi SUJ ...
    1989 Volume 86 Issue 5 Pages 1089-1095
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Endotoxemia frequently appears in severe type of alcoholic liver disease. However, we have little knowledge how endotoxin influences the progression of alcoholic liver injury. Thus, to study the causal mechanism for the progression to the sever type of alcoholic liver diseases, endotoxin (0.2mg/100g BW, E. Coli O26:B6) was intravenously injected in chronic ehtanol-fed rats and controls, and then, rats were sacrificed after 16 hours of endotoxin treatment. The elevation of serum GOT and GPT activities induced by endotoxin was significantly higher in chronic ethanol-fed rats than controls, and these biochemical changes were well correlated with the grade of necrosis of liver histology. Furthermore, in chronic ethanol-fed rats, endotoxin treatment tremendously increased blood BUN and creatinine levels and produced the degeneration of renal tubuli with neutrophil infiltration into glomerulus. These experimental findings are very similar to the severe type of alcoholic liver disease. On the other hand, endotoxin significantly decreased serum values of CH50 in chronic ethanol-fed rats, but not in controls. Such alterations of CH50 induced by endotoxin were well correlated with the several parameters indicating the injury of liver and kidney. Therefore, the present study may indicate that chronic ethanol ingestion aggravates endotoxin-induced organ injury, and that the activation of complement system may associate with such progression of organ injury.
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  • Shigeki ONO, Fuminori MORIYASU, Nabuyuki BAN, Takashi TAMADA, Toshihik ...
    1989 Volume 86 Issue 5 Pages 1096-1102
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We used magnetic resonance imaging (MRI) at 0.2 T and angiography to evaluate hepatocellularcarcinoma (HCC) in 43 patietns who had not received therapy. Tumor detection rate by MRI was 84%, T2-weighted spin echo imaging being the best detection method, though one case could only be detected by T1-weighted imaging (Inversion Recovery method).
    The signal intensity of the tumor relative to the surrounding liver parenchyma was studied using MRI, and tumor staining using angiography. Where there was a high signal intensity in the T2-weighted spin echo images there was a corresponding tendency for tumor staining to be marked.
    MRI demonstrated the inner structure-which resembles random high-intensity threads on a lower-intensity background-of hypervascular tumors. Tumors with capsules appeared with the capsule clearly dilineated irrespective of the signal intensity of the tumor itself.
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  • Yoko NOMURA
    1989 Volume 86 Issue 5 Pages 1103-1113
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Nucleation time (NT) and growth time (GT) were measured in gallbladder bile of patients with cholesterol gallstones. NT was significantly shortened (NT<10 days) in pure cholesterol stones but was moderately shortened (11_??_NT_??_21) in mixed and combination stones. GT also was accelerated (GT<7 days) in cholesterol stones. NT was shortened in increased biliary total protein, but on the contrary, was shortened in decreased apo A-I. NT of bile by UDCA therapy but not CDCA was extended. This suggests that increased apo A-I during UDCA therapy might imply extension of NT. The strong negative correlation between GT and CSI of bile suggests that CSI plays an important role in crystal growth.
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  • Shigeki LEE, Naotaka FUJITA, Go KOBAYASHI, Katsumi KIMURA, Hiromitsu W ...
    1989 Volume 86 Issue 5 Pages 1114-1119
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Superselective angiography of the cystic artery was attempted with 18 patients for the purpose of improving angiographic diagnosis of gallbladder diseases. The success rate of cystic angiography using a fine catheter (5 Fr. size) and a specially coated guidewire was 72% (13/18). In comparison with usual hepatic angiography findings obtained by cystic angiography proved easier to evaluate, because the angiograms thus obtained avoided visualization of the hepatic artery and showed good opacification of the fine branches of the cystic artery.
    It is concluded that angiographic diagnosis of gallbladder diseases can be improved by use of superselective cystic angiography.
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  • Susumu NAKATA
    1989 Volume 86 Issue 5 Pages 1120-1124
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Endoscopical lithotomy was carried out in 14 patients with cholecystolithiasis. 6 patients were men and 8 were women (age 40-78). 4 of them underwent endoscopical lithotomy because of their inoperability. The other patients refused surgical operation and wished the procedure.
    Percutaneous Transhepatic Cholecystodrainage (PTCCD) was performed at the right intercostal space under ultrasonical guide and was dilated to 16 Fr in diameter. After 1-2 weeks, lithotomy by using Percutaneous Transhepatic Cholecystoscopy (PTCCS) started. Nd-YAG laser and/or Electrohydraulic lithotripter were used to fragment stones. Lithotomy was successfully finished without serious complications in all cases.
    In conclusions, the therapeutic advantage of PTCCS may be expected for cholecystolithiasis.
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  • Takaharu OISHI, Masao NAKAGAWA, Tadao BAMBA, Shiro HOSODA
    1989 Volume 86 Issue 5 Pages 1125-1135
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Pancreatic injury was induced to rats with intraperitoneal injection of ethionine 60mg per 100g BW twice or three times weekly for 6 weeks. These rats were given 100mg/kg of Camostat mesilate (CM) via a gastric tube daily for 14 days. CM administration resulted in an increase of pancreatic wet weight, hypertrophy and hyperplasia of acinar cells, and an increase of exorine pancreatic function. Acini prepared from CM and ethionine-treated rats exhibited increased response to caerulein, but decreased sensitivity to caerulein. The plasma CCK level in rats with CM administration 24 hours later was higher than that without CM administration. However, there were no significant changes in plasma CCK and secretin level thereafter.
    We concluded that CM had a trophic effect on the pancrease with ethionine-induced pancreatic injury, and CCK was considered playing the same role in injured pancreas as the normal rat pancreas. Studies using CCK receptor antagonist are needed for further clarification.
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  • Akihiro FUNAKOSHI, Masayuki FURUKAWA, Yukio YAMADA, Hideyuki WAKASUGI, ...
    1989 Volume 86 Issue 5 Pages 1136-1140
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We tested the new radioimmunoassay method of serum phospholipase A2 (PLA2). In healthy individuals, serum PLA2 concentrations were 301±65.6ng/dl (mean±SD), and in patients with acute pancreatitis, significant elevations of serum PLA2 concentrations were observed. In clinical course of acute pancreatitis, serum PLA2 was maintained high level more longer than serum amylase and elastase 1. In patients with chronic pancreatitis, serum PLA2 concentration were low at a stage of severe exocrine dysfunction, and high at a stage of acute exacerbation. In patients with pancreatic cancer, serum PLA2 concentration were changed in accord with severity of disease states. After endoscopic retrograde pancreatography, serum PLA2 levels immediately elevated significantly, and returned to basal levels 24 hours later. Serum PLA2 concentrations were within normal range in patients with other malignant tumors, diabetes mellitus, chronic liver diseases, and hypertension, whereas in patients with chronic renal failure serum PLA2 concentrations were elevated. These results suggest that measurement of serum PLA2 can be clinically useful for diagnosis of pancreatitis and monitoring of mild and severe stage of pancreatitis.
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  • Hiroyasu KAWAKAMI, Norio SAWABU, Yasuhiro TAKEMORI, Takashi OKAI, Yosh ...
    1989 Volume 86 Issue 5 Pages 1141-1148
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to evaluate the usefulness of sialyl SSEA-1(SLX) as a tumor marker of digestive cancers, serum levels of the antigen were determined in 334 patients with malignancies and 196 patients with benign diseases. The results indicated that positivity of the antigen in sera from malignant patients was highest in pancreatic cancer (58%) and biliary tract cancer (56%). False positive incidence of SLX in sera from benign diseases was as low as 6%, revealing low false positivity. Comparison with other tumor markers such as CA19-9, CA-50, CEA and ST-439 showed that positivity of SLX was as high as that of CEA, whereas it was lower than that of CA19-9 or CA-50. On the other hand, false positivity of SLX as well as ST-439 was lowest, and accuracy of SLX was no less high than that of CA19-9 or CA-50. In sera of pancreatic and biliary tract cancer, positive incidences of CA19-9, CEA and ST-439 were 80%, 64% and 53%, respectively, and the diagnostic effeciency increased by combined assay of SLX with CA19-9 (88%), CEA (81%) and ST-439 (71%).
    SLX appeares to be no less useful than the other recently developed carbohydrate antigens or CEA as serum tumor marker for pancreatico-biliary cancer.
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  • Hidechika HASHIMURA, Michiyo TANAKA, Kouki KAWAI, Ryuji FUKUSHIMA, Kiy ...
    1989 Volume 86 Issue 5 Pages 1149-1153
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hiroto TAKEMOTO, Toshihito SEKI, Yoshitsugu KUBOTA, Masaru OKUHIRA, Sh ...
    1989 Volume 86 Issue 5 Pages 1154-1158
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Tadanori NAGAI, Ryuzo KOYAMA, Yutaka SASAGAWA, Wataru NISIMURA, Kazuno ...
    1989 Volume 86 Issue 5 Pages 1159-1163
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Akihito TORII, Hiroyuki SUENAGA, Yuichi SUZUKI, Tetsuya OKUDA, Yasuhir ...
    1989 Volume 86 Issue 5 Pages 1164-1167
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Susumu TAZUMA, [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1989 Volume 86 Issue 5 Pages 1168
    Published: 1989
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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