Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 87, Issue 12
Displaying 1-20 of 20 articles from this issue
  • Investigation of the histamine receptor
    Hitoshi KONDO, Norio TANI, Takeshi MIWA
    1990 Volume 87 Issue 12 Pages 2579-2586
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Investigation about the histamine receptors of gastric smooth muscle cells was done using isolated gastric smooth muscle cells of guinea pig. Isolated gastric smooth muscle cells were prepared from the stomach of guinea pig by percoll density gradient centrifugation after enzymatic digestion with collagenase and dispase. Contraction of the cells was determined by image of phase-contrast microscopy and digitizer and expressed as the mean per cent of control in cell axis length. The concentration of intracellular Ca2+([Ca2+]i) was measured with the calcium-sensitive dye fura-2. Tested drugs were added to the incubation medium containing 2mM CaCl2. Both contractile response and [Ca2+]i increased following histamine stimulation in a dose-dependent manner from 10-7 to 10-4 M, and those peaks were obtained in application of 10-4 M pyridylethylamine, a H1 receptor agonist, made an increase in [Ca2+]i as much as 10-4 M histamine. But 10-4 M dymaprit, a H2 receptor agonist, made only small increase in [Ca2+]i, whose value was similar to that in application of only 2mM CaCl2. 10-4 M pyrilamine, a H1 receptor antagonist, inhibited the response to 10-4 M histamine, and the value was similar to that in application of only 2mM CaCl2. Cimetidine, a H2 receptor antagonist, did not inhibit the response to 10-4 M histamine stimulation at all.
    From those results it is concluded that the action of histamine to the gastric smooth muscle cells of guinea pig may be mediated through H1 receptor, but not through H2 receptor.
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  • Nobuo YOSHIKAWA
    1990 Volume 87 Issue 12 Pages 2587-2593
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    To investigate the effects of 14 days administration of H2-receptor antagonist (famotidine) on gastric gastrin and somatostatin secretion, bombesin and glucagon were perfused in the isolated pancreas-duodenum deprived rat stomach. Then serum gastrin concentration, gastric mucosal gastrin and somatostatin content, and gastric mucosal G-cell and D-cell numbers were examined.
    The 14 days administration of famotidine caused the significant increase of basal gastrin secretion, antral G-cell hyperplasia, high gastrin sensitivity to the stimulation by bombesin, and the low somatostatin sensitivity to the stimulation by glucagon. These facts would suggest that 14 days famotidine administration disturbed not only gastrin secretion but also somatostatin secretion. These results may contribute, at least in part, to the high recurrense of ulcers after withdrawal of H2-receptor antagonists.
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  • Yasuo SAKAI
    1990 Volume 87 Issue 12 Pages 2594-2604
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Quantitive measurement of the liver fatty acid binding protein (L-FABP) in human gastrointestinal tract was carried out by the single radial immunodiffusion method, and its. tissue distribution was also determined. L-FABP existed only in the epithelial absorptive cells of duodenum, jejunum, ileum and colon, but not in esophagus and stomach. Jejunum showed the highest content of L-FABP at the level of 1.5% of the cytosolic protein. These findings support the concept that FABP has a role in the fatty acid absorption and transport. Intestinal L-FABP content revealed no difference between patients given ordinary diet and intravenous hyperalimentation, although earlier report by experimental animal showed that oral high fat loading increased the L-FABP concentration. L-FABP concentration had no corelation with the duration of IVH, but was well corelated with serum triglyceride and total-cholesterol levels. The colon of normal controls showed similar level of L-FABP concentration to that of distal intestine. L-FABP content in the colorectal cancer tissues and that in the specimen of the ulcerative colitis was also assayed, the former showed similar level to, and the latter showed significantly lower level than that in normal controls.
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  • Takeshi URABE
    1990 Volume 87 Issue 12 Pages 2605-2613
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    We examined serologically and immunohistochemically the new carbohydrate antigen CA-50 to clarify the mechanism of its high serum value and clinical significance in several liver diseases. The subjects included 145 patients with benign liver diseases and hepatocellular carcinoma (HCC). The serum CA-50 value was high in chronic active hepatitis with lobular disorganization, liver cirrhosis and HCC. It was not correlated with serum levels of GPT nor γ-GTP. Immunohistochemical analysis revealed that proliferated bile ductules showed mainly positive staining in all subjects, whereas hepatoma cells were negative. The proliferated bile ductules with positive staining for CA-50 were quantified by an original method. The number of the proliferated bile ductules with positive staining for CA-50 was significantly correlated with the serum CA-50 value (r=0.62, P<0.05). In the FPLC analysis, there was no significant difference between the expression pattern and molecular weight of CA-50 in liver diseases and pancreatic cancer. Also no difference in the carbohydrate structure that coexisted with CA-50 was detected in the ConA or LCA affinity column study. It was suggested that the increase of carbohydrate antigen CA-50 in several liver diseases might reflect the proliferation of bile ductules, and that the structure of CA-50 in benign liver diseases dose not differ from that of CA-50 from patients with pancreatic cancer.
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  • The measurement method in vitro and the effect of hepatocytes upon the phagocytic activity
    Shuichi NIIMOTO, Hiroshi SHIMADA, Gizou NAKAGAWARA
    1990 Volume 87 Issue 12 Pages 2614-2622
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The measurement method of the phagocytic activity of Kupffer cells (=KC) was investigated and the effect of hepatocytes (=HC) upon the phagocytic activity of KC was studied in vitro.
    Phagocytic activity of KC was measured by the uptake rate of 59Fe by KC (2×106) incubated with chondroitin sulfate 59Fe for 24 hours (=phagocytic activity rate: PAR). When KC were co-cultured with HC (2×105, 5×105, 1×106 cells) for 24 hours, the PAR increased in proportion to the increase in number of HC (4.13, 5.37, 6.28%), comparing with that of KC alone (3.36%). And when KC were cultured with the supernate of HC (5×105) which were cultured for 24 hours, this PAR (4.15%) was superior to that of KC alone, but didn't exceed that of KC co-cultured with HC (5×105).
    The PAR was remarkably suppressed by the inhibitor of glycolysis (NaF), but was not supressed so much by the inhibitor of oxidative phosphorylation (NaN3).
    The measurement method of phagocytic activity of KC in vitro was established, and it was proved that the PAR was enhanced by co-cultured HC and the supernate of clutured HC, and depended on glycolytic metabolism.
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  • Shigeki ONO, Fuminori MORIYASU, Takashi TAMADA, Toshihiko KAWASAKI, Yu ...
    1990 Volume 87 Issue 12 Pages 2623-2628
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    We examined the 1H-NMR spectra of the lipid region of human plasma according to the method of Fossel et al. as the specific blood test for cancer. The study group included 20 patients with hepatocellular carcinoma (HCC), 10 patients with liver cirrhosis(LC)and 10 normal controls (NC). We measured the linewidths of methyl and methylene resonances of lipoprotein lipids at a proton resonance frequency of 400 MHz using a JNM GX400 FT NMR spectrometer. The mean linewidth values and the standard deviation of methyl were as follows: 41.3±7.2Hz (NC), 34.8±6.9Hz (LC), 29.6±5.7Hz (HCC). There was thus a significant difference between NC and HCC. On the other hand, there was no significant difference between the three groups in the case of methylene. Although the spectrum in the HCC and LC groups had a blunt head but narrow body, that of the NC group had a pointed head but wide body, and the linewidth values thus showed wide dispersion. This difference in spectral patterns between the two groups was characteristic. Linewidth values, however, did not correlated well with the laboratory data.
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  • Naoaki TANNO, Chiyuki NAKANOME, Hideo YAMAZAKI, Masayoshi SASAKI, Waic ...
    1990 Volume 87 Issue 12 Pages 2629-2634
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    We have carried out a comparative study of the diameter of main pancreatic duct (MPD) on endoscopic retrograde pancreatography (ERP) with the frequency of detection of MPD by computed tomography (CT) in order to clarify the importance of MPD appearance on CT in the pancreatic and biliary diseases. The normal MPD on ERP was demonstrated by CT in a low frequency. MPD was most frequently observed in the pancreatic body on CT. The dilatation of MPD on ERP was found in both moderate and advanced pancreatitis group. However, the significant demonstration of MPD by CT was found in advanced group alone. We observed that CT finding of dilated duct correlated with that on ERP in advanced group alone.
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  • Nobuhiko SOYAMA, Harumasa OHYANAGI, Yoichi SAITOH
    1990 Volume 87 Issue 12 Pages 2635-2641
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Lymphocytes obtained from regional lymph nodes and spleen in the patients with gastrointestinal carcinoma were fused with the human B lymphoblastoid cell line GC01 and human hybridomas producing human monoclonal antibody (MoAb) were derived. Human MoAb No. 235 (IgM) derived from spleen cell of a gastric cancer patient reacted with adanocarcinoma of stomach, colon, and pancreas in the new immunohistochemical assay, modified direct immunoperoxidase method, and reacted with KATO III cells in cultured cell lines. The antigenetic determinant of this antibody was suspected to be protein moiety after enzyme treatment. The competitive binding inhibition assay indicated that its epitope was different from anti-CEA monoclonal antibodies (KM10, A10, B9, AH3, JA4) and KM01.
    These findings suggested the possible use of human MoAb No. 235 for clinical application of targeting cancer chemotherapy in the future.
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  • Shinji HASEGAWA, Akinori KASAHARA, Atsushi OKUNO, Michikazu KONO, Shin ...
    1990 Volume 87 Issue 12 Pages 2642-2645
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Toshio ONIZUKA, Miyuki SHIMIZU, Hiroshi HOSHINO, Osamu TAKAHARA, Atsus ...
    1990 Volume 87 Issue 12 Pages 2646-2650
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Tetsuo YAMAMOTO, Michiko SHABANA, Yasuo NOSAKA, Haruhiko KOJO, Kunihik ...
    1990 Volume 87 Issue 12 Pages 2651-2655
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Ryushi TAZAWA, Yuji OKA, Yuichi NAKAMURA, Atsushi TAKAHASHI
    1990 Volume 87 Issue 12 Pages 2656-2659
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Hiroshi ISHII, Kazushige ARAI, Tsutomu KAETSU, Tsukasa ITOU, Tohru SAT ...
    1990 Volume 87 Issue 12 Pages 2660-2664
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Hiroyuki KURODA, Junko TAJIMA, Yuhji SAKAKIBARA, Taijirou ISHIYAMA, Ma ...
    1990 Volume 87 Issue 12 Pages 2665-2668
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Masafumi KOMATSU, Mitsuo GOTO, Shin-ichi ISHIDA, Makoto NIIZAWA, Hidea ...
    1990 Volume 87 Issue 12 Pages 2669-2674
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Yoji ANAMI, Yuji NIMURA, Nobuki KAMEOKA, Junichi KAMIYA, Shoji MAEDA, ...
    1990 Volume 87 Issue 12 Pages 2675-2679
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • M Masuda, M Maruyama, A Tanaka, S Kawamoto, Y Fujita, T Sanayama
    1990 Volume 87 Issue 12 Pages 2680-2684
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Yukio YAMADA, Hiroaki ISHIKAWA, Daisuke YASUDA, Takuya TSUTSUMI, Tetsu ...
    1990 Volume 87 Issue 12 Pages 2685-2689
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Masamitsu HARADA, [in Japanese], [in Japanese], [in Japanese]
    1990 Volume 87 Issue 12 Pages 2690
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Masatoshi KUDO, Shusuke TOMITA, Hiroshi KASHIDA, Jun MIMURA, Yoshihiro ...
    1990 Volume 87 Issue 12 Pages 2691
    Published: 1990
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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