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Mizuhiro MOGI, Masaki KITAJIMA, Tateo KIUCHI, Hayato KAWAKAMI, Hiroshi ...
1990 Volume 87 Issue 5 Pages
1131-1138
Published: 1990
Released on J-STAGE: December 26, 2007
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Many studies have postulated the role of gastric acid and mucosal microcirculation in the development of gastric ulcer. We considered tat gastric mucosal glycoprotein played one of te most important roles as the defensive factors of te gastric mucosa. The purpose of this study is to investigate te location and distribution of glycoprotein in the gastric mucosa in normal and stressful condition.
Male Wistar rats, weighing 250g were used as the experimental animals. Alternation and distribution of glycoproteine in the gastric mucosa were studied by light and electron microscopic examination employing HRP-labeled lectin such as PNA, DBA, ConA, RCA, SBA, WGA and UEA-1 as a parameter befor and after the development of stress ulcer. Around Tirty percent scalding burn of the rat body surface area was created. At 2, 5 and 24 hours after burn induction, the gastric mucosa was examined.
PNA-binding sites which were primarily localized to the generative cell zone in the control rat appeared to extend from the upper layer to the intermediate or the basal layer of the gastric mucosa in accordance with the development of stress ulcer after burn.
Most of the binding sites was detected in the parietal cells particularly along microvilli in the intracellular secretory canaliculi. The tubulovesicular system in the parietal cells became stainable with PNA in the stress-loaded rats. Alternation and binding pattern of lectin, especially of PNA may indicate the local response of the parietal cells of the gastric mucosa against the stress.
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Hogen TEI, Tadakazu SHIMODA, Masahiro IKEGAMI, Saburo OHSHIBA
1990 Volume 87 Issue 5 Pages
1139-1145
Published: 1990
Released on J-STAGE: December 26, 2007
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We had clinicopathologically studied early cancer-like adanced gastric cancer in relation to peptic ulcer (UI). Early cancer-like advanced cancers with the difficulty to distinguish from early gastric cancer were selected for materials. Result were as followed:
1) Early cancer-like advanced cancers were consisted of 133 lesions, of which 128 lesions (96.2%) had peptic ulcer in cancerous lesion (73 lesions were active stage and 55 lesions were scarring stage).
2) Early cancer-like advanced cancers were 17.0% in all advanced gastric cancers. Proper muscle cancer (in which depth of cancerous invasion is up to proper muscle coat) was more common in early cancer-like advanced cancers than in Borrmann type advanced cancers.
3) Early cancer-like advanced cancers with peptic ulcer showed wide intramucosal cancerous infiltration. Thus, average of maximum diameter was 51.8mm.
4) Those ulcer were commonly reaching to proper muscle coat or subserosa. Submucosal fibrosis was prominent and scattered proliferation of tumor cells were often seen within fibrosis.
5) Extent of cancerous infiltration in mucosa was more wide than that in submucosa.
The above findings have led us to consider that early cancer-like advanced cancers have grown because of high degreed submucosal fibrosis by the deep ulcerations due to"malignant cycle".
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Shinji OHASHI, Yoshihisa TSUKAMOTO, Hidemi GOTO, Satoshi HASE, Yasumas ...
1990 Volume 87 Issue 5 Pages
1146-1153
Published: 1990
Released on J-STAGE: December 26, 2007
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The subjects were 64 cases of gastric cancer. In all 64 cases, resected specimens (formalin fixed) were subjected to endoscopic ultrasonographic examination by the water immersion method in order to assess the horizontal spread of the invasive gastric cancer. Ultrasonographic findings were compared with the histopathological findings of resected specimens. The tumor which invaded the submucosa or deeper layers was visualized as low echogenicity region in the third layer (corresopnding to the submucosa) or deeper layers. The horizontal spread of the low echogenicity region represented that of the tumor or the associated fibrosis, but not the tumor itself. In the cases showing echo patterns which were characterisic of the peptic ulceration in the tumor focus (Type II-1, II-2, UL), the horizontal spread of cancer invasion in the submucosal layer or deeper layers (L Ca) was smaller than that of low echogenicity region depicted by ultrasonography (L U). In the other (Type II-3, III, A, B) cases, L Ca was almost comparable to L U.
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Minoru KIDA, Ko TANAKA, Fumio HATTORI, Seiji KONDOU, Wataru HARADA, At ...
1990 Volume 87 Issue 5 Pages
1154-1159
Published: 1990
Released on J-STAGE: December 26, 2007
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We histologically compared 3 types of adenoma and cancer of the large intestine using 620 adenoma specimens (509 Is type lesions, 83 Ip type lesions, and 28 flat type lesions) and 113 specimens of early stage cancer (51 Is type lesions, 39 Ip type lesions, and 23 flat type lesions) obtained during the past 5-year period at our department. More than 90% of the Is and Ip type polyps were adenoma or carcinoma in adenoma while 25.5% of the flat elevations were m or sm carcinoma. Flat elevations even less than 10mm in diameter were frequently carcinomas (26.3%) compared with the other types (both Is and Ip types, 6.7%), and all of those 10mm or more in size were carcinomas. The distribution of the flat type early cancers in the large intestine was similar to that of advanced cancer with high percentages of carcinoma at each site compared with the other types. These results suggest that the carcinogenesis and progression of flat type early stage cancer differ from those of the other types.
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Kiyokazu TAKEMURA, Takeshi OBARA, Kiyoshi OKAMURA
1990 Volume 87 Issue 5 Pages
1160-1167
Published: 1990
Released on J-STAGE: December 26, 2007
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The expression of epidermal growth factor (EGF) was studied immunohistochemically in a total of 92 human colorectal cancers to examine whether EGF was expressed in relation to its degrees of malignancy. Significantly high incidence of EGF was observed in advanced cancers than in early cancers. In the tissues of advanced cancers, tumor cells having EGF-immunoreactivity tended to be more frequently and intensely observed in deeply invasive tumor cells than in supperifical tumor cells. Regarding macroscopic and histologic types, incidence of EGF-immunoreactivity was significatly higher in infiltrating types and in differentiated types than in localized types and in poorly differentiated types, respectively. Cases with poor prognosis and in advanced stages showed high positive rate of EGF-immunoreactivity. These results suggest that the expression of EGF may serve as a biologic marker for the degree of malignancy in human colorectal cancers.
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Takeaki NAGAMINE, Shuichi SAITO, Shoji YAMADA, Mieko KANEKO, Masahiro ...
1990 Volume 87 Issue 5 Pages
1168-1174
Published: 1990
Released on J-STAGE: December 26, 2007
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In order to evaluate the clinical significance of serum biotin and biotinidase in liver disease, serum biotin levels and biotinidase activities were determined in 83 patients with various liver diseases and 10 healthy controls. Serum biotin levels and biotinidase activities were determined by a simplified lactobacillus plantarum bioassay and liquid chromatography with fluorimetric detection respectively.
Serum biotin levels in decompensated liver cirrhosis, hepatoma and fulminant hepatitis were found to be significant low compared with healthy controls, while it was significant high in autoimmune hepatitis. There was no significant difference between serum biotin levels in the other liver diseases and healthy controls.
In various liver diseases except for both acute hepatitis and alcoholic liver disease biotinidase activities were significantly reduced than in healthy controls. Serum biotinidase activities were correlated with serum albumin, prothrombin time, ChE and total cholesterol respectively, suggesting that biotinidase activities may reflect the degree of liver damage.
These results seem that biotin deficiency may occur in some cases of severe liver diseases.
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Akitoshi KAKISAKA, Shinichi KASAI, Mitsuhiro INAGAKI, Yasuyuki SUZUKI, ...
1990 Volume 87 Issue 5 Pages
1175-1182
Published: 1990
Released on J-STAGE: December 26, 2007
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We studied protective effects of Dibutyryl cyclic AMP (DBcAMP) which is a permeable form of cyclic AMP on ischemic liver failure (ischemic time: 90 minutes).
Mongrel dogs were used. A portal-systemic bypass was established by inserting a heparinized catheter between the splenic vein and the femoral vein. Acute liver failure was induced by the en bloc clamp of the porta hepatis.
In the treated group, 0.1mg/kg/min (total dose, 300mg) of DBcAMP was injected intravenously from three hours before the clamp to the end of the experiment. The control group was injected physiological saline. Effects of DBcAMP were evaluated according to survival rates, blood pressure, serum biochemical findings, hepatic blood flow, ATP levels in liver tissues and histological findings.
The survival rates were 16% in the control group, and 88% in the treated group. The mean blood pressure after reperfusion was rapidly decreased in the control group while in the treated group, it was maintained nearly 100mmHg.
Serum biochemical findings, hepatic blood flow and tissue ATP levels were significantly improved in the treated group. On histological findings, necrosis with bleeding was observed one day after ischemia in the control group while in the treated group mild chronic ischemic change without necrosis was observed on two weeks after ischemia.
In conclusion, ischemic liver failure was apparently protected by the administration of DBcAMP.
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Naohiro YAMASHITA
1990 Volume 87 Issue 5 Pages
1183-1190
Published: 1990
Released on J-STAGE: December 26, 2007
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Recently, methyl tertiary butyl ether (MTBE) has been evaluated as a new cholesterol gallstone dissolving agent. The dissolution rate of cholesterol monohydrate (ChM) by MTBE was measured and effective types of MTBE administration in vivo were studied.
When the dissolution rate of ChM by MTBE was compared with that of d-limonene and monooctanoin used clinically at present, MTBE was found to be the most excellent dissolving agent. However, the dissolving effect of MTBE can be suppressed by water (a component of wet gallstones and 97% of bile) because MTBE is insoluble in water. Therefore, MTBE mixed with a small amount of surfactants (MTBEP) was prepared. The dissolving effect of MTBE alone and MTBEP on dry and wet gallstones were measured. The dissolving effect of MTBE alone on the wet stones was markedly decreased, while MTBEP suppressed the decrease of dissolving effect on wet stones significantly. Furthermore, the dissolving effect of MTBE alone and MTBEP on gallstones in water were measured. MTBEP significantly improved the decrease of dissolving effect on stones by MTBE alone, because the addition of a small amount of a surfactant suppressed the influence of water for dissolving effect of MTBE. MTBE emulsion, newly type of solvent, was prepard. Although the dissolution rate of ChM in MTBE emulsion was low, the dissolving effect of MTBE emulsion was also better than that of MTBE in animal experiments. In this study, emulsion type can be expected to become excellent dissolving agents, because emulsion type suppressed the influence of water for dissolving effect of solvent.
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Analysis of autopsy cases
Wataru KIMURA, Akira KURODA, Yasuhiko MORIOKA
1990 Volume 87 Issue 5 Pages
1191-1202
Published: 1990
Released on J-STAGE: December 26, 2007
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Using 800 autopsy cases (422 men, 378 women, mean age 78.7) mainly consisting of the aged, we made a clinicopathological analysis of endocrine tumors of the pancreas. The results were: 1) Endocrine tumors and similar lesions were found in 24 cases (25 lesions). 20 lesions (20 cases) were determined as tumors and five lesions (five cases) as hyperplasia of Langerhans islet. 2) Prevalence of tumor was 10% (6/60) in the all-section group and 1.6% (12/738) in the three-section group. Grossly observed tumor was in a single case. 3) As for the size of tumors, 35% of them were 1, 000μm or smaller. All of the five cases of hyperplastic lesions were 1, 000μm, or smaller in size. 4) None of the cases with tumors and hyperplastic lesions showed symptoms of excessive hormone. 5) In immunohistochemical analysis, hormone production was observed in all of the 20 cases of tumor and multiple hormone production was found in 14 of them (70%). 5) Ductular or tubular structures were found in or adjacent to tumor in 12 cases (60%) and hyperplasia in one case (20%). 6) One lesion each of tumor and hyperplasia were found in a single case severally. Langerhans islets, 500μm or larger in size, were found in three lesions of tumor (15%). Futhermore, langerhans islets with the diameter of mean + 2SD or larger were found around five lesions of tumor (25%) and three lesions of hyperplasia (60%). Above findings suggest that endocrine tumors of the pancreas is hyghly prevalent and most of them do not go beyond asymptomatic tumors (non-functional in the broad sense of meaning) with no symptoms of excessive hormone though they produce some hormones. And it is indicated that some of the endocrine tumors or hyperplasias develop from totipotent stem cells of duct epithelia, and factors that promote the growth of Langerhans islets might exist.
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Yukinori SASAKI, Masao KOBARI, Ryuji NAKAMURA, Seiki MATSUNO
1990 Volume 87 Issue 5 Pages
1203-1211
Published: 1990
Released on J-STAGE: December 26, 2007
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Experimental chronic pancreatitis model were made in five dogs with chronic pancreatic fistula by injection of microspheres into peripheral pancreatic duct. Sequential changes of pancreatic exocrine and endocrine functions also with morphology were studied. Significant decrease of volume, bicarbonate output, and amylase output of pure pancreatic juice (PPJ) separately collected with stimulation of secretin were detected. While, viscosity of PPJ was significantly increased with concomitant increase of hexosamine concentration. Chronic pancreatitis was demonstrated morphologically also. These results suggest that concentrated PPJ caused by decrease in volume in PPJ and increase in viscosity of PPJ with concomitant increase in hexosamine concentration bring about the progression of chronic pancreatitis in this experimental model.
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Atsushi NONAKA, Tadao MANABE, Takahisa KYOGOKU, Koichiro TAMURA, Takay ...
1990 Volume 87 Issue 5 Pages
1212-1216
Published: 1990
Released on J-STAGE: December 26, 2007
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In order to clarify the role of free radicals in the pathogenesis of acute pancreatitis, we observed the effect of a new synthetic free radical scavenger (CV-3611) on the pathological state in the models of both caerulein and CDE-diet induced acute pancreatitis in mice. In both models of acute pancreatitis, the levels of serum amylase activity were reduced significantly by the treatment of CV-3611. Pancreatic edema formation was also reduced significantly at 3.5 and 9h after the first caerulein i.p. injection.
The 4 days survival rate in CDE-diet induced pancreatitis was significantly elevated from 41.2% to 81.3% by the treatment of CV-3611. These results indicate that this synthetic scavenger, which has a long circulation half life, high affinity to biomembrane and good cell penetration ability, is effective on the development of both severe and mild pancreatitis. The main pathogenesis of both models is suggested to be radical reactions on the biomembrane which is caused by the interreaction between endothelium and neutrophile in caerulein induced pancreatitis, and by the lipid peroxidation on the biomembrane of the organella in the cell in CDE-diet induced pancreatitis.
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Shigekazu HAYASHI, Jun-ichi KANOH, Tuyoshi FURUKAWA, Yoshihiro KAGA, A ...
1990 Volume 87 Issue 5 Pages
1217-1222
Published: 1990
Released on J-STAGE: December 26, 2007
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Yasushi ADACHI, Tsuyoshi YABANA, Tomoko KOHRI, Shingo ICHIYANAGI, Sada ...
1990 Volume 87 Issue 5 Pages
1223-1227
Published: 1990
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Kazuo NOTSUMATA, Haruhito TSUTIYA, Tadao YAMAZAKI, Masayoshi ITO, Yuki ...
1990 Volume 87 Issue 5 Pages
1228-1231
Published: 1990
Released on J-STAGE: December 26, 2007
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Yasuharu SAITO, Seiji NARUMIYA, Hirohiko AOKI, Mitsukuni MIZUNO, Akihi ...
1990 Volume 87 Issue 5 Pages
1232-1235
Published: 1990
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Review of Japanese literature
Kouji KUNIEDA, Masaru OKUHIRA, Tsuneyuki NONAKA, Kazuhiro TANI, Toshia ...
1990 Volume 87 Issue 5 Pages
1236-1242
Published: 1990
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Ken KAJIURA, Kazuhiko FUJIKI, Masahiro YAMADA, Tsuyosi TAKENAGA, Ichiz ...
1990 Volume 87 Issue 5 Pages
1243-1247
Published: 1990
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Shinichi KISO, Takeshi KASHIHARA, Atsushi OHKI, Eijiro FUJIMORI, Fusao ...
1990 Volume 87 Issue 5 Pages
1248-1252
Published: 1990
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Tomomi TABATA, Makoto USAMI, Hidekazu OHMIYA, Harumasa OHYANAGI, Youic ...
1990 Volume 87 Issue 5 Pages
1253-1257
Published: 1990
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Akitaka TANAKA, Norishige OZEKI, Yoshitugu TAKAHASHI, Shigenori ITO, Y ...
1990 Volume 87 Issue 5 Pages
1258-1262
Published: 1990
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Yukiya YOSHIDA, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
1990 Volume 87 Issue 5 Pages
1263
Published: 1990
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Hiroji NISHINO, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
1990 Volume 87 Issue 5 Pages
1264
Published: 1990
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