Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 88, Issue 1
Displaying 1-20 of 20 articles from this issue
  • [in Japanese]
    1991 Volume 88 Issue 1 Pages 1-2
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Keiichi OHTSU, Shiro FUKUMOTO, Yoshihiro SHIMADA
    1991 Volume 88 Issue 1 Pages 3-11
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    In healing process of indomethacin (IND)-induced gastric mucosal lesion of rats, cell kinetics in the cells from margin of mucosal lesion, background mucosa and pyloric gland mucosa were investigated with histochemistry using bromodeoxyuridine.
    Results obtained were as follows; 1) In marginal cells of mucosal lesion, cell number of s-phase expressing the cell renewal abruptly increased 6 hours after IND injection, showed maximal value at 24 hours and kept higher value even at 72 hours, when the lesions were in scar stage.
    2) In background mucosal cells, the cell number of s-phase showed higher value at 12 hours after IND injection, although statistically not significant, and significantly lower value at 24 and 48 hours after IND injection.
    3) Some s-phase cells were observed in the surface epithelial cells covering the mucosal defect.
    4) In pyloric gland mucosal cells, there was no remarkable change in cell number of s-phase during the experimental period.
    It was concluded that in healing process of acute gastric mucosal lesion, there was a time lag between the macroscopic observation and cell kinetics in normalization of s-phase cell number.
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  • Nobuhiko HAYASHI, Sunao KAWANO, Shingo TSUJI, Harumasa YOSHIHARA, Koui ...
    1991 Volume 88 Issue 1 Pages 12-18
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Although acute gastric mucosal lesions (AGMLs) develop after the mucosal ischemia and reperfusion, precise intracellular mechanism for the development of AGMLs remains unclear. In the present paper, we investigated intracellular calcium level, lipid peroxidation, and gastric mucosal lesion in rat hemorrhagic shock model. We estimated intracellular calcium by measuring phosphorylase a activity in the gastric mucosa.
    The mucosal phosphorylase a increased during hemorrhagic shock, while it returned to be normal after the reinfusion of shed blood. Although mucosal lipid peroxide and area of AGMLs did not increase significantly during hemorrhagic shock, they increased significantly after the reinfusion. Diltiazem, a calcium antagonist, prohibited both of the increase in mucosal phosphorylase a activity during hemorrhagic shock and the increase in lipid peroxide content and area of AGMLs after the reinfusion of the shed blood. Organ reflectance spectrophotometry revealed that the gastric corpus suffered mucosal ischemia and hypoxia during the hemorrhagic shock, . The are of AGMLs had a good correlation with a level of lipid peroxidation in the gastric mucosa.
    These results suggest 1) that intracellular free calcium in gastric mucosa increases during the mucosal ischemia; 2) that a calcium antagonist prohibits the increased intracellular free calcium during mucosal ischemia; and 3) that a calcium antagonist prohibits the increased mucosal lipid peroxide and the development of AGMLs after the mucosal reperfusion. Thus it is concluded that the increased intracellular free calcium during ischemia plays a key role in the mucosal tissue injury in the ischemia-reperfusion state.
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  • An evaluation by follow-up cases
    Hiroto HORINOUCHI, Eiichi SATO, Suguru YONEZAWA, Sadao TANAKA
    1991 Volume 88 Issue 1 Pages 19-27
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Alterations of carbohydrate chain antigens were investigated immunoshitochemically in relation to histological malignant changes on 62 cases of gastric actypical epithelial lesions (adenomas) which were diagnosed as Group-III at the first biopsy and then followed up more than one year. Among 7 carbohydrate chain antigens which are related to Lewis antigens, sialyl LeX-i antigen showed the most impressive findings; The positivity percentage of the first biopsy specimens of Group-III was 6%, however, it raised to 33% in the final biopsy specimens of Group-IV, 50% in the resected specimens of border-line lesions, and 67% in the resected specimens of carcinomas. The results indicate that there exsists a close correlation between malignant change of gastric atypical epithelial lesions and alteration of carbohydrate chain in terms of sialylation.
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  • K. WADA, H. NISHISAKI, Y. KONDA, T. MATOZAKI, O. NAKANO, K. MATSUDA, M ...
    1991 Volume 88 Issue 1 Pages 28-32
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The muscarinic receptor system involved in perpsinogen secretion from isolated guinea pig gastric chief cells was investigated by assessing the effect of muscarinic receptor antagonists on carbamylcholine (CCh)-induced pepsinogen secretion. CCh stimulated pepsinogen secretion in a dose dependent manner with the maximal and the half-maximal stimulatory concentrations at 10-4 and 3×10-6M, respectively. Each of five different muscarinic receptor antagonists such as atropine, pirenzepine, 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP), AF-DX116 and scopolamine reduced pepsinogen secretion stimulated by graded concentration of CCh, but did not alter the maximum secretion. The increase in concentration of each antagonist caused parallel rightward shift of the dose response curve to CCh. Schild analysis of the inhibition of CCh-induced pepsinogen secretion by the antagonists showed that pA2 values of atropine, scopolamine and 4-DAMP are 8.8, 9.2 and 9.0, respectively. On the other hand, pA2 values of pirenzepine and AF-DX116 are 6.5 and 5.9, respectively, suggesting that the muscarinic receptore mediating pepsinogen secretion from chief cells has a intermediate affinity for pirenzepine and a low affinity for AF-DX116. These results suggest that the muscarinic acetylcholine receptor mediating pepsinogen secretion from gastric chief cells is the M3 subtype
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  • An analysis in view of cumulative risk of recurrence and reoperation
    Yuji FUNAYAMA, Iwao SASAKI, Hiroo NAITO, Yasuhiko KAMIYAMA, Michinaga ...
    1991 Volume 88 Issue 1 Pages 33-39
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Forty nine cases with Crohn's disease operated at our department were analyzed in view of cumulative risk of reoperation and recurrence.
    Cumulative risk of reoperation was 22% at five years and 40% at ten years. Cumulative risk of recurrence after "curative" resection in thirty four cases was 53% at five years and 73% at ten years. Both risks were the highest in ileal type and the lowest in colonic type. Cumulative risk of relapse in ileocolic type after non-"curative" resection was 67% at five and ten years, which showed no significant difference with cumulative risk of recurrence after "curative" resection. Cumulative risk of recurrence after "curative" resection with proximal resection margin longer than 10cm had no significant difference from the group with shorter margin. Cumulative risk of recurrence and reoperation after reoperation was a little higher than after initial operation, but they showed no statistical difference.
    In conclusion, there is no need to stick to "curative" resection, and it is enough to have the resection margin no longer than 10cm. It is essential not to spoil the quality of life by excessive resection.
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  • Validation as a measure of hepatic functional reserve
    Masatoshi KUDO, David R. Vera, Robert C. Stadalnik, Walter L. Trudeau, ...
    1991 Volume 88 Issue 1 Pages 40-50
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    [Tc-99m] Galactosyl-neoglycoalbumin (TcNGA) is a synthetic radiolabeled ligand specific to the hepatocyte receptor, hepatic binding protein (HBP), a specific receptor to serum asialoglycoprotein.
    A TcNGA study was performed on 34 humans: normal volunteers (7), chronic hepatitis (6), hepatic cirrhosis (8), and hepatocellular carcinoma superimposed on cirrhosis (13). Heart and liver time activity curves were obtained following intravenous injection of TcNGA (5mCi, 1.82×10-9mol/kg). HBP concentration ([HBP]) was calculated by curve-fitting techniques using the nonlinear three compartment model, which includes bimolecular reaction between HBP and TcNGA. [HBP] values were compared with conventional liver function tests.
    [HBP] had a good correlation with prothrombin time (n=34, r=0.694, p=0.0001) thrombotest (n=34, r=0.692, p=0.0001), hepaplastin test (n=26, r=0.787, p=0.0001), albumin (n=34, r=0.712, p=0.0001), cholinesterase (n=34, r=0.801, p=0.0001), ICGR15 (n=33, r=-0.761, p=0.0001), KICG (n=30, r=0.709, p=0.0001), ICG Rmax (n=12, r=0.735, p=0.0064) and Child-Turcotte classification score (n=34, r=-0.819, p=0.0001).
    We concluded that excellent correlations of [HBP] to conventional liver function tests suggest that in vivo receptor measurement via TcNGA kinetic analysis is a sensitive and promissing method in the estimation of hepatic functional reserve in patients with chronic liver disease.
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  • Hitoshi AMAKASU, Atsushi KANNQ, Masahide ABE, Katsuro SATO, Yoshio GOT ...
    1991 Volume 88 Issue 1 Pages 51-56
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The renal tubular damage in liver cirrhosis was evaluated by measurement of urinary N-acetyl-β-D-glucosaminidase (NAG) activity in 32 patients with various stages of liver cirrhosis. The patients were divided to three groups (A, B, and C) according to Child's criterion and urinary NAG activity and renal function parameters were compared in these groups. As a result, urinary NAG activity in group C was significantly higher than group A and group B and slight elevation of serum BUN level and low creatinin clearance value were also observed in group C. In 8 patients who developed the renal failure in their clinical course the level of urinary NAG activity was markedly elevated in the early phase of renal failure. These results suggested that the measurement of urinary NAG activity was useful for early diagnosis of renal impairment in the cirrhotic patients.
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  • Hiroaki MIYAOKA
    1991 Volume 88 Issue 1 Pages 57-64
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    The levels of beta 2-microglobulin (β2m) in peripheral blood mononuclear cells (PBMC) and livers from patients with chronic liver diseases type B were measured. β2m of liver and PBMC in chronic active hepatitis was higher than those of controls (p<0.01, p<0.01). β2m of PBMC are directly proportional to those of livers (r=0.746), β2m levels of PBMC in patients with chronic active hepatitis during the exacerbation of hepatitis was higher than those of remission of hepatitis. The levels of β2m in PBMC in vivo, was significantly increased during either inteferon α or β administration. Interferon-γ positive cells in the liver were exacted in the β2m increased group of chronic hepatitis type B. INF-γ production in the lymphocyte of livers, may play an important role in the occurence of liver injury in patients with chronic hepatitis type B.
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  • Masazumi OGAWA, Ikuta NAKANO, Hitoshi FUNATOMI, Yoshio HATTA
    1991 Volume 88 Issue 1 Pages 65-70
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Under low choline diet, chronic pancreatic injury was induced by the intraperitoneal administration of ethionine of 10mg/100g body weight, 3 times a week for sequential 8 weeks in rats and histological changes, pancreatic hydroxyproline and collagenase activity were studied. Histologically, acute inflammatory changes were observed mainly in the early days, and infiltration of fibroblastoid cells was increased on the 28th day. Then, fat droplets deposition became remarkable. Though hydroxyproline concentration increased gradually after the injection of ethionine and showed maximal level on the 28th day when the slight fibrosis was noticed, it was decreased on the 56th day when collagenous tissue was replaced by fat droplets. On the other hand, collagenase activity showed two peaks, in the early phase and on the 56th day, and it showed bottom level on the 28th day. Collagen synthesis relatively dominated over degradation on the 28th day. It is suggested that the unbalance of collagen metabolism between synthesis and degradation may result in fibrosis.
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  • Hitoshi FUNATOMI, Yoshio HATTA
    1991 Volume 88 Issue 1 Pages 71-76
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
    Heat stability of human serum phospholipase A2 (PLA2) was investigated comparing to those of porcine pancreatic PLA2 (p-PLA2) and PLA2 in human duodenal aspirate.
    PLA2 activity was measured by radiochemical method using phosphatidylcholine as a substrate.
    While the activities of PLA2 in duodenal aspirate and p-PLA2 solution were not influenced by the heat treatment of 55°C, PLA2 activity in normal human serum was remarkably decreased by the preincubation at 55°C for only 5 minutes.
    From the result of preincubation temperature, serum PLA2 activity began to decrease at 50°C and the decrease of PLA2 activity was remarkable above 55°C of preincubation temperature.
    As a conclusion, PLA2 in human serum was consisted of heat labile and stable form, and latter was thought to be derived from pancreas. Thus serum PLA2 level should be assessed with consideration of incubation temperature of PLA2 assay and whether pretreatment of heating was done or not.
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  • Eiji HAMAJIMA, Toshio ASAI, Syozo OKAMURA, Shinnji OOHASHI, Hisayoshi ...
    1991 Volume 88 Issue 1 Pages 77-81
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Hitoshi TAKAHASHI, Yoshikuni OKAMURA, Shyokaku HASHIMOTO, Akihiro KAWA ...
    1991 Volume 88 Issue 1 Pages 82-86
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Yuji MATSUBAYASHI, Yoshio IZUMI, Yumiko MORI, Kyoko ITOH, Atsuo MORIOK ...
    1991 Volume 88 Issue 1 Pages 87-90
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Toshio HOASHI, Sumio TSUDA, Tsuneyoshi YAO, Toshihiko ICHIMARU, Tsukas ...
    1991 Volume 88 Issue 1 Pages 91-95
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Ei SASAKI, Atsushi TOYONAGA, Shunji MURAYAMA, Shinichi KUBOYAMA, Sugur ...
    1991 Volume 88 Issue 1 Pages 96-99
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Satoshi ITANO, Ryuichiro MAEKAWA, Yukihiko URAMOTO, Hiroyuki KOIKAWA, ...
    1991 Volume 88 Issue 1 Pages 100-104
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Naotsuka OKAYAMA, Tadahisa MIYAMOTO, Takasi KUROKAWA, Naotomo OONO, Si ...
    1991 Volume 88 Issue 1 Pages 105-110
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Masamichi KATO, Yuji NIMURA, Junichi KAMIYA, Katsushi OKAMOTO, Shoji M ...
    1991 Volume 88 Issue 1 Pages 111-114
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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  • Noriyuki ARIMA, Yukimasa YAMASHITA, Tomoyuki MORISHITA, Takuo FUJITA, ...
    1991 Volume 88 Issue 1 Pages 115
    Published: 1991
    Released on J-STAGE: December 26, 2007
    JOURNAL FREE ACCESS
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