Serum and ascitic phospholipase A
2 (PLA
2) activities in rat acute pancreatitis induced by sodium taurocholate (TCA) were investigated with special reference to their heat stabilities and were compared to those in serum of carrageenan induced granuloma in rats.
PLA
2 activity was measured by previously reported radiochemical method and was separated into two forms, heat stable and labile PLA
2, based on the stability to preincubation at 55°C for 5 minutes. While a heat stable PLA
2 predominantly increased in ascitic fluid, the elevations of both heat stable and labile PLA
2 activities in serum were observed in TCA induced pancreatitis.
On the other hand, serum PLA
2, which was mainly composed of heat labile form, elevated with the increase of leukocyte count in carrageenan induced granuloma rats.
These facts suggested that serum PLA
2 activity might increase in acute inflammatory changes other than in pancretic diseases. And it is assumed that PLA
2 derived from extrapancreatic origins as well as pancreatic secretory PLA
2 may also contribute to high PLA
2 level in acute pancreatitis, because pancreatic secretory PLA
2 was generally accepted to be heat stable.
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