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Shinji OHASHI, Toshio ASAI, Shozo OKAMURA, Eiji HAMAJIMA, Hisayoshi IT ...
1991 Volume 88 Issue 7 Pages
1397-1403
Published: 1991
Released on J-STAGE: December 26, 2007
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The aim of this study was to assess the accuracy and limitation of Endoscopic ultrasonography (EUS) in the preoperative evaluation of the cardiac cancer (cancer in the area less than 2cm from the esophago-gastric junction). EUS was preoperatively performed in 20 patients with cardiac cancer. These cases were visualized by the water immersion method and balloon contact method. The results were correlated with the histology of resected specimens. EUS was accurate in assessing the depth of tumor infiltration. Over all accuracy of EUS for cardiac cancer was 90%. Recognition of ultrasonographic patterns had enabled us to judge the mode of tumor infiltration whether expansive or infiltrative. Microscopical spread over 5mm to the esophagus of the cardiac cancer could be detected by EUS. EUS was accurate in diagnosing thoracic lymph nodes (No. 110, 111, 112) metastasis of the cardiac cancer (accuracy 86%). EUS was valuable in the clinical diagnosis of the cardiac cancer.
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Kunio SUZUKI, Hajime MURAKITA, Masaki HAGINO, Fukiko SATOU, Takuji KAT ...
1991 Volume 88 Issue 7 Pages
1404-1412
Published: 1991
Released on J-STAGE: December 26, 2007
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Histo-pathological appearances of DMH-induced colonic tumor in Wistar rats were sequentially observed upto the 35th week after the drug administration. In our series, 28 tumors were successfully induced in the colonic mucosa of 19 out of 64 rats treated with DMH, and they were histologically diagnosed as adenocarcinoma. However, there were differences in the histo-pathological findings of the carcinoma between the distal colon and the proximal colon in rats. That is, the slowly growing type of well differentiated adenocarcinoma was likely to originate from the proper mucosa in the distal colon, while in the proximal colon the rapidly growing type of tumor did from atypical glands in the lymphoid follicles. Therefore, it was suggested that histogenesis and growing processes of the carcinoma were differed in the distal colon from that in the proximal colon in rats.
Second, epithelial mucosubstances and lectin-binding properties of these lesions were examined histochemically. There were differences in the lectin-binding patterns of UEA-I and PNA between carcinomas and/or atypical glands in the lymphoid follicle and normal background mucosa in rat colon. In the UEA-I staining, almost all tumors were positively stained except for one case, and in the well differentiated type a positive staining was discernible at the cell apex and secretory product in tumors, while in the undifferentiated type, it was seen at the cytoplasma and secretory product. From these histopathological and histochemical studies it may be concluded that these lectins is likely to be useful as tumor markers for the large bowel, and also effective for a diagnosis of minute carcinoma in the large bowel.
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Shigeto MORIMOTO
1991 Volume 88 Issue 7 Pages
1413-1419
Published: 1991
Released on J-STAGE: December 26, 2007
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Plasminogen activator (PA) activity of the gastric mucosa of liver cirrhotics was measured by use of Glt-Gly-Arg-MCA as the substrate. Gastroduodenal mucosal lesions were more frequently seen in cirrhotic patients with red spot of the gastric mucosa (RS (+) group) as compared to the cases without red spot (RS (-) group). RS (+) group had poor liver function as compared to RS (-) group. PA activity of the gastric mucosa in RS (+) group was higher than RS (-) and control groups.
Furthermore, the elevated PA activity was higherly assosiated with severe liver damage estimated by routine liver function test and Child's classification.
Separatively quantitative assay of PAs was performed by using Lysine-Sepharose 4B chromatography. Both the tissue type and urokinase type PA were higher in the RS (+) group than in control group.
These results suggest that accelerated tissue fibrinolytic activity is one of the causative factors of gastric mucosal bleeding in cirrhotic patients with red spot.
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Hideki HAGIWARA, Norio HAYASHI, Eiji MITA, Keiji UEDA, Tetsuo TAKEHARA ...
1991 Volume 88 Issue 7 Pages
1420-1425
Published: 1991
Released on J-STAGE: December 26, 2007
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We estimated HCV RNA in serum of 20 patients with chronic hepatitis C during interferon therapyusing a combination assay of reverse transcription and polymerase chain reaction (RT-PCR). RNA was extracted from 200μl of serum. The primers were chosen from the NS3 region of prototype HCV RNA sequence. After 40 cycles of PCR, the products were analyzed by Southern hybridization. HCV RNA was detected in 18 of 20(90%) patients before therapy. In cases with improvement of serum GPT level, HCV RNA became undetectable at 4 weeks of the therapy. However, HCV RNA reappeared within 4 weeks after the therapy in cases with relapse. In the no response group, HCV RNA did not disappear during the therapy. Interferon therapy is beneficial in improvement of viremia of HCV, and the detection of HCV RNA in serum is useful to evaluate the antiviral effect of interferon.
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Ritsuro USUI, Hideo ISE, Osamu KITAYAMA, Noriyoshi SUZUKI, Seiki MATSU ...
1991 Volume 88 Issue 7 Pages
1426-1435
Published: 1991
Released on J-STAGE: December 26, 2007
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In order to investigate the pathogenesis of black gallstones associated with hemolytic disorders, experimental and clinical studies were performed. Firstly, a one-shot injection of bilirubin and hemolyzed blood, and a continuous injection of bilirubin were performed in mongrel dogs with the aim to analyze the changes of hepatic and gallbladder bile composition. Secondly, gallbladder bile obtained intraoperatively from the patients with black gallstones associated with hemolytic disease, patients with black stones not associated with hemolytic disease and patients with gallbladder polyp were analyzed in order to compare the characteristics of hemolytic patients' bile with those of the model animals. High concentration of total bilirubin and increased proportion of bilirubin monoconjugate (BMC) fraction was observed in the bile of patients with black stone associated with hemolytic disease compared with the bile of the patients of other two groups. Bile pH, total calcium concentration and lionized calcium concentration were similar in all the groups. Total bile lipid concentration of hemolytic patients was decreased but the ratio of total bile acid to total lipid was increased. Both bolus injection of bilirubin and hemolyzed blood produced an increase of total bilirubin concentration and BMC in hepatic bile. Continuous bilirubin injection for 7 days induced an increase of total bilirubin and BMC in both hepatic and gallbladder bile. These results suggest that the conjugating capacity of the hepatocyte is surpassed by the excessive amount of bilirubin produced after hemolysis, producing therefore, an increased BMC and unconjugated bilirubin content in bile. This increased content can precipitate in bile as black gallstones.
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A prospective analysis of 70 initial cases
Kenichi IDO, Ken KIMURA, Chiaki KAWAMOTO, Shinichi SATOH, Norio ISODA, ...
1991 Volume 88 Issue 7 Pages
1436-1441
Published: 1991
Released on J-STAGE: December 26, 2007
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Laparoscopic cholecystectomy using Nd-YAG laser was performed in 70 consecutive patients with preoperatively symptomatic gallstones. Sixty-eight patients had successful completion of the Laparoscopic cholecystectomy. Mean hospital stay was 7.8 days (range: 4-12), and a diet as well as physical walking was tolerated in all 68 patients by the next day following the procedure. Mean operating time was 216 minutes in the initial 10 cases, which improved significantly to 87 minutes in the latest 28 cases. The difference of operative time did not influence any difference on the postoperative course. Nineteen of 68 patients (27.9%) required postoperative pain medication (pentazocine 15mg, i.m.). Two patients required conversion to open cholecystectomy because of a pin hole injury on the portal vein by laser quartz in the first case, and difficult dissection due to severe adohesion in the 46th case. There is a definite learning curve in this new modality. Laparoscopic cholecystectomy is a safe and effective procedure, significantly improving the quality of life of the patients with gallstones.
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Takashi MATOZAKI, Choitsu SAKAMOTO, Hogara NISHISAKI, Yoshitaka KONDA, ...
1991 Volume 88 Issue 7 Pages
1442-1446
Published: 1991
Released on J-STAGE: December 26, 2007
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The effects of cholecystokinin (CCK) and other pancreatic secretagogues on phosphatidylcholine (PC) synthesis were studied in isolated rat pancreatic acini. When acini were incubated with [
3H] choline in the presence of 1nM CCK-octapeptide (CCK8) for 60min, the incorporations of [
3H] choline to both water soluble choline metabolites and PC in acini were reduced by CCK8 to 74% and 41% of control, respectively. Pulse-chase study revealed that CCK reduced both the disappearance of phosphocholine and the synthesis of PC. Ca
2+-mobilizing secretagogues such as carbamylcholine and Ca
2+ ionophore A23187 also reduced PC synthesis to the same extent as CCK8. By contrast, neither cAMP-dependent secretagogues such as secretin and dibutyryl cAMP nor a phorbol ester had any effect on PC synthesis in acini. These results suggest that CCK inhibits PC synthesis by inducing both the reduction of choline uptake into acini and the inhibition of CTP: phosphocholine cytidylyltransferase activity. This hormonal regulation of PC synthesis via CDP-choline pathway appears to be mediated by Ca
2+-dependent pathway but not by cAMP- or protein kinase C-dependent pathway.
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Kohjiro OHTSUKA, Yosiya MISHINA, Kiyoto YAMAMOTO, Masayuki KAWAI, Jin ...
1991 Volume 88 Issue 7 Pages
1447-1453
Published: 1991
Released on J-STAGE: December 26, 2007
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Kazunari ISHII, Yoshiki HASEGAWA, Kazunori MAEDA, Yukiko NAKAHARA, Yas ...
1991 Volume 88 Issue 7 Pages
1454-1458
Published: 1991
Released on J-STAGE: December 26, 2007
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Hiroshi KANAGAWA, Satoaki MIMA, Harukazu KOUYAMA, Hitosi MIZUO, Masahi ...
1991 Volume 88 Issue 7 Pages
1459-1462
Published: 1991
Released on J-STAGE: December 26, 2007
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Masahiro IRIE, Mamoru MURAOKA, Hideki TESHIMA, Kazunori MINE, [in Japa ...
1991 Volume 88 Issue 7 Pages
1463-1468
Published: 1991
Released on J-STAGE: December 26, 2007
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Yoshinori SATOH, Hiroshi SERIZAWA, Naoki AIKAWA, Shingo HORI, Jun-ichi ...
1991 Volume 88 Issue 7 Pages
1469-1473
Published: 1991
Released on J-STAGE: December 26, 2007
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Akinori KASAHARA, Toshiaki NAGAKURA, Masahide OSHITA, Michikazu KONO, ...
1991 Volume 88 Issue 7 Pages
1474-1478
Published: 1991
Released on J-STAGE: December 26, 2007
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Toshihiko KOJIMA, Yoko OHIRA, Kathuyuki ISHIBASHI, Masahito KURODA, To ...
1991 Volume 88 Issue 7 Pages
1479-1484
Published: 1991
Released on J-STAGE: December 26, 2007
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Susumu OHWADA, Yukio MIYAMOTO, Masaaki TAKESHITA, Seiji NAKAMURA, Yosh ...
1991 Volume 88 Issue 7 Pages
1485-1489
Published: 1991
Released on J-STAGE: December 26, 2007
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Toshifumi NEKOHASHI, Susumu MORIMOTO, Jujin SATOI, Yoshinari KATO, Ryo ...
1991 Volume 88 Issue 7 Pages
1490-1494
Published: 1991
Released on J-STAGE: December 26, 2007
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Toshikazu OHNUMA, Naoharu TAKEYAMA, Hirohiko YAMASE, Masahiro YAMADA
1991 Volume 88 Issue 7 Pages
1495-1499
Published: 1991
Released on J-STAGE: December 26, 2007
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
1991 Volume 88 Issue 7 Pages
1500-1503
Published: 1991
Released on J-STAGE: December 26, 2007
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Percutaneous transhepatic embolization of shunt
Ryuhei OGAWA, Mitsuo TSUBAKIMOTO, Hiromi HASHIMOTO, Haruki NAKATSUKA, ...
1991 Volume 88 Issue 7 Pages
1504-1508
Published: 1991
Released on J-STAGE: December 26, 2007
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A case report with special reference to possible role of accessory hepato-biliary duct in stone formation
Hitoshi TAKAHASHI, Yoshikuni OKAMURA, Naoaki AKIZAWA, Shyokaku HASHIMO ...
1991 Volume 88 Issue 7 Pages
1509-1514
Published: 1991
Released on J-STAGE: December 26, 2007
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