We succeeded in establishing a rectal cancer cell line RKK-YK from the primary lesion in a patient with rectal cancer. It took 36.2 hours for duplication. We were able to transplan the RKK-YK cell line to nude mice at a transplantation rate of 50%. The transplanted tumor exhibited histological features similar to those of the primary lesion. Cancer cells with two different degrees of differentiation, in which features of moderately differentiated adenocarcinoma and well-differentiated adenocarcinoma were observed together, were established. The levels of the tumor markers (CEA, CA19-9 and AFP) were elevated in the supernatant of the culture solution and the serum of the nude mice over time course. In the immunohistological examination of the transplanted tumor, anti-CEA, anti-CA19-9 and anti-AFP antibodies were positively stained. Molecular biological analysis revealed nor point mutation or deletion in K-ras gene exon 1 and 2, p53 gene exon 5 to 11 or MCC.
To study the characterization of hepatic bile mucus glycoprotein in hepatolithiasis cases, we isolated glycoprotein from the bile of patients with intrahepatic gallstones by gel filtration and ultracentrifugation. The carbohydrate chains of mucus glycoprotein were released by hydrazinolysis. The properties of the hepatolithiasis carbohydrate chains were compared with those of a control group. The mucus glycoprotein concentration in bile in hepatolithiasis patients (299.9μg/ml) was higher than that in the controls (28.0μg/ml). Both groups had no evident changes in molecular weight or amino acid composition. The quantities of N-Galactosamine and sulfate in the hepatolithiasis group were larger than in those of the control group. Carbohydrate chain lengths in the hepatolithiasis group were shorter than those in the control group. This phenomenon suggests that mucus glycoprotein of the bile in hepatolithiasis patients has a different metabolism and biological function from that of normal bile.