By the use of methylene blue resin 2 G, causes of the discrepancy between the results of the tube and tubeless tests were investigated, and the rate of disagreement between the results of the tubeless and gastric screening tests was discussed.
The results can be summarized as follows:
1. As for dye liberation in methylene blue resin solutions with various pH values, the amount of the free dye showed a sudden decrease above pH 2. From a viewpoint of the rate of urinary dye excretion after the introduction of 10 mg of methylene blue dye into the stomach, there is the great possibility that hypoacidity in the tube gastric method is interpreted as anaacidity in tubeless gastric analysis.
2. Methylene blue resin 2 G was repeatedly administered to the same cases in an attempt to study the urinary excretion of the dye. It was found that the greater the excretion was, the more marked was a fluctuation in gastric acidity. Fluctuations between hypoacidity or normal acidity and hyperacidity were noticed in 9 (60%) out of 15 cases. This can be considered to be responsible for the fact that the method in question is limited to qualitative analysis.
3. From time-to-time changes in the free dye in the stomach due to the oral administration of methylene blue resin 2 G, discrepancies were seldom caused by the shortening of the time of contact between gastric juice and resin attributable to the hyperperistalsis of the stomach. The effects of the impaired secretion of the dye from the stomach into the intestine seem rather profound.
4. Even if organic pyloric stenosis is not present, caffaine sodium benzonate administration augmented gastric secretion in hyperacidity, thereby causing pyloric occlusion in a reflexive manner. Thus this case sometimes is assessed as false negative.
5. The rete of disagreement concerning anacidity was high with the tubeless gastric analysis procedure in hepatic and renal diseases. In liver disease there was a correlation between B. S. P. values and the rate of disagreement. Even if the B. S. P. values were normal in renal disease, false negative cases were frequent. This finding is in agreementwith the report of Rodman et al.
6. That anacidity in the tube test showed normal acidity in the tubeless test seems to be attributable to great quantities of the dye liberated by intestinal juice.
7. Analyses by regions of the rate of disagreement between the results of the gasteic screening and tubeless tests showed that improvements were made possible by thoroughgoing instructions regarding the oral administration of resin and the method of urine collection. Errors in collection time can be reduced by increasing the amount of resin to be orally administered.

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Various factors of ascites production and its absorption were studied in so-called Mckee's dogs of which vena cava inferior were constricted at the part of 2 or 3 cm above the diaphragm. Observations had been made on these dogs for four weeks after the procedure of the constriction. The following results were obtained:
1) Portal venous pressure, abdominal and thoracic inferior vena cava pressure of Mckee's dogs with ascites (peritoneum closed) were considerably higher than that of the no ascites series (peritoneum opened). These pressure difference were mainly due to the results of accumulation of ascites.
2) Total flow of liver lymph was remarkably increased (approx, 10 times) in ascitic dogs than that in normal dogs.
3) Liver lymphgraphy revealed the existence of intrahepatic lymph duct and its dilatation in ascitic dogs.
4) Increased permiability of liver capillary in ascitic dogs were proved by injected various kinds of dyes.
5) The electrophoretic pattern for serum, liver lymph, and ascitic fluid were shown to be practically similar.
6) These local facters indicated that the liver and liver capsel lymph can be accounted for the source of ascitic fluid.
7) On the other hand, total protein, alubumin percentage, A/G ratio, and colloid osmotic pressure of serum were studied as general factors, They were decreased in their values. Sodium in serum increased. The factors were not primary for production of ascites, but secondary.
8) Absorbability of peritoneal cavity were studied with dyes which were injected intraperitonealy under various conditions. The dye absorption through peritoneal cavity of Mckee's dog was found to be remarkably disturbed than that in normal dog.
9) The mechanism of the accumulation of ascites would be understand as a result of unbalance between over producted liver lymph and disturbed absorption through peritoneal cavity.

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