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Volume 24, Issue 8
Displaying 1-19 of 19 articles from this issue
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Part 8. The Enzymic Transformation of Amylopectin into AmyloseBunji MARUO, Tsuneo KOBAYASHI, Yutaka TSUKANO, Nobuhiro YAMADA1950 Volume 24 Issue 8 Pages 347-349
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe transformation of glutinous rice starch into amylose by the action of amylosynthease was proved with certainty, using the potentiometric iodine titration, commenced by Bates, French, and Rundle3). The enzymic formation of amylose from potato amylopectin was also indicated by the same method. The rough calculations from the titration curves have shown the yields of amylose formed from amylopectin-type polysaccharides to be as great as about 75% of the subst-rates. In order to explain the high yields of amylose, the mode of action of the enzyme, previously suggested by the authors, might well be again postulated.View full abstractDownload PDF (290K) -
Part 1. On the Enzyme Activity of Internal OrgansR. SASAKI, S. ODAGIRI1950 Volume 24 Issue 8 Pages 350-352
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe activities of pepsin-, trypsin-. lipase- and amylase-like enzymes were examined on various sorts of fishes. It resulted that the internal organs of mackerel, bonito and sardine are the most suitable as raw materials of trypsin among fishes used in these experiments.View full abstractDownload PDF (220K) -
Part 2. On the Separation of Protease from the Internal Organs of FishR. SASAKI, S. ODAGIRI1950 Volume 24 Issue 8 Pages 352-354
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSEnzyme powder is prepared from internal organs of fish as follows: fresh pyloric coeca containing much protease is crushed with sand in the mortar, water is added, enzyme is extracted, and clear extract is obtained by filtration or centrifugation. After, Japanese acid clay is added to this solution, clay is separated by centrifugal separation and dried in a desiccator. The enzyme can be eluted from this prepared clay by slightly alkaline buffer solution.View full abstractDownload PDF (252K) -
Part 2. Flavors resulting from Heating. (1) Isolation of an Acetal, C10H22O3Tamotsu YOKOTSUKA1950 Volume 24 Issue 8 Pages 355-358
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSBy mesns of steam distillation of newly pressed Soy-cake, a sort of Soy-flavours (bp105mm 33.0-33.5° 150_??_170mm 43_??_44°) was isolated. Judging by the elementary analysis and the depression of freezing point, the author estimates its empirical formula as (C10H22O3+2H2O).
The value of its molecular refraction agrees with the constitutional formula ((CH3)2CHCH2CHOHCH(OC2H5)2+2H2O).View full abstractDownload PDF (373K) -
Part 3. Flavors resulting from Heating (2) The Constitutional Formula of C10H22O3 and C9H20O2Tamotsu YOKOTSUKA1950 Volume 24 Issue 8 Pages 358-362
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAs shown in the previous reports two kinds of Soy-flavors, C9H20O3 (bp120mm 33°) and C10H22O3+2H2O (bp105mm 33_??_33.5°) were isolated. Judging by their decomposition products by heat, HCl, KMnO4, or Ag2O, the author estimates the constitutional formula of the former to be isovaleraldehyde diethyl acetal (CH3)2CHCH2CH(OC2H5)2 and that of the latter to be α-hydroxy-isocaproaldehyde diethyl acetal (CH3)2CHCH2CHOHCH(OC2H5)2.View full abstractDownload PDF (465K) -
Part 3. The Transformation of β-Isopropyl-D-glucoside-tetraacetateYoshiyuki INOUYE, Konoshin ONODERA, Ikuo KARASAWA, Yoshihiko NISHISAWA1950 Volume 24 Issue 8 Pages 362-365
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS2, 3, 4, 6-Tetraacetyl-β-isopropyl-D-glucopyranoside was transformed into the isomeric alpha form, 2, 3, 4, 6-tetra acetyl-α-isopropyl-D-glucopyranoside, by the action of SbCl5 which had been found previously by the authors to be suitable for promoting the transformation of acetylated beta glucosides into their alpha isomers.
Under the influence of SbCl5 solution in chloroform at 10°, the beta form, melting at 105° and having a specific rotation in chloroform of -23.5°, was easily isomerized to the alpha form which was isolated in 64 percent yield as a crystal, having melting point 56° and specific rotation -124.7° in chloroform.
The observed value of rotation of the alpha form agrees well with the value calculated by HUDSON's second isorotatipn rule, therefore it is concluded that the oxide ring of the alpha form is the same as the ring of the beta form.View full abstractDownload PDF (376K) -
Part 1. Identification of Amino Acids by Paper ChromatographyYoichi ÔTAKA1950 Volume 24 Issue 8 Pages 366-368
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAmino acids contained in Saké are studied by paper chromatography. 15 amino acids are identified and their contents (%) in Saké are roughly calculated by comparing, the chromatography patterns of sample Saké and pure amino acids mixture. 12 amino acids were found in Saké formerly, and this time serine, glycine and valine are newly found by the present Author.View full abstractDownload PDF (260K) -
Part 1. Paper Chromatography of Copper Salts of Amino AcidKazuo MIURA1950 Volume 24 Issue 8 Pages 369-371
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSLutidine (2, 6-dimethylpyridine) was used as a solvent for the separation of copper salts on paper partition chromatograph method, for this is superior to other solvents (phenol, isoamyl alcohol, . n-butanol, s-collidine).
As the result of this experiment, the Rf values of copper salts increased with the rise of temperature, and the separation was not completed. The desirable temperature is 18_??_20°C.
For making the samples, insoluble copper salts were dissolved in pyridine, in that case, pyridine did not affect the Rf values of copper salts. The Cu-pyridine gave an obscure spot which was separated from the amino acids (copper salts).
The contents of water in lutidine influenced the Rf values of copper salts considerably, so it is desirable to saturate lutidine with water completely.View full abstractDownload PDF (242K) -
Takao ÔMURA1950 Volume 24 Issue 8 Pages 371-376
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. Labile phospate compounds containing easily acid-hydrolysable phosphate and other esters were examined in the varieties of higher plants. Except soy bean cotyledons, none of these gave positive results.
2. The labile phosphate compound in soy bean cotyledon was purified according to the methode of DOUNCE et al.(14) for the isolation of ATP from rabbit muscle. The analytical data and adsorption spectrum suggested that it was a nucleotide, but owing to the incompleteness of its purification, the structure and other properties could not yet be identified.View full abstractDownload PDF (1221K) -
Takeshi HARA, Takeo TANAKA, Yahisa KOTAKE, Tetsuo ISHIKAWA, Taro NIIDA1950 Volume 24 Issue 8 Pages 377-380
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) With an idea of blowing the air in a fermenter into broth repeatedly by rotating a special agitating tube, We planned an appratus for submerged fermentation.
(2) By this small apparatus we obtained nearly the same rapidity of fermentation which has been shown in the industrial preparation of sorbose, penicillin and streptomycin.
(3) Concerning the volume of air supplied into the fermenter, it was quite enough when the voulume of 1/2_??_1/5 of that of the broth per minute. (The minimum air requirement for this apparatus was not determined)
(4) There was no trouble about the foaming during all the period of fermentation.View full abstractDownload PDF (357K) -
Part 1. On the Nitrate Reductase of Tobacco and SilkwormHirohisa ÔMURA1950 Volume 24 Issue 8 Pages 380-382
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe fresh leaves of tobacco plants were ground and centrifuged. I can confirm the existence of a nitrate reductase in the supernatant fluid obtained. Nitrates eaten disappear in the tissues of silkworms. They are reduced to nitrites by the ground worm tissues.View full abstractDownload PDF (291K) -
Parto 3. Alkoholfermentado de AcidekstraktoSin'itirô KAWAMURA1950 Volume 24 Issue 8 Pages 383-385
Published: 1950
Released on J-STAGE: November 21, 2008
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Parto 4. Ingredienco de la Resta_??_o de Protein-ekstraktadoSin'itirô KAWAMURA1950 Volume 24 Issue 8 Pages 385-388
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS -
1950 Volume 24 Issue 8 Pages 385E-385
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (97K) -
Kazuo YAMAFUJI, Ikuko YOSHIHARA1950 Volume 24 Issue 8 Pages 388-391
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSOn the basis of experiments with sterilized silkworm eggs and heated mulberry leaves the mechanism of virus formation was. discussed in connection with the nitrogen metabolism in living tissues.View full abstractDownload PDF (471K) -
Part 1. Influence of Acetoxime on the Action of Catalase and Protease in Yeast CellsToshihiko AKITA1950 Volume 24 Issue 8 Pages 391-392
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe catalatic activity of living yeast cells is hindered by acctoxime. The relation between this hinderance and concentration of oxime was studied. The protease activity of yeast cells, however, is elevated by the same oxime.View full abstractDownload PDF (186K) -
Part 2. Action of Acetoxime on Tobacco Plants and Silkworm LarvaeToshihiko AKITA1950 Volume 24 Issue 8 Pages 393-394
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIf tobacco plants are suitably treated with an acetoxime solution, a kind of stripes appears on their leaves. It has not'yet been determined whether these striped leaves contain any infectious agents or not.
Some further experiments concerning the silkworm virus production under sterilized conditions were performed with acetoxime and the results of earlier experiments were confirmed.View full abstractDownload PDF (194K) -
T. YABUTA, Y. SUMIKI, K. FUKUNAGA, M. HORIUCHI1950 Volume 24 Issue 8 Pages 395-397
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn Part 21, the reaction of ultra-violet ray against the physiological activity of gibberellin was examined. The activity of gibberellin was determined by measuring the heights of the steals of young rice seedlings which were germinated and cultured from the seeds treated with gibberellin.
The result was as follows, although it could not be shown so quantitatively: By irradiation of ultra-violet ray during 60 minutes, the physiological activity of gibberellin disappeared quitely in the 0.0002% solution and decreasd to about a half in the 0.0004% solution.
In Part 22, rice seedlings were cultured by treating with or without gibberellin and general components of these seedlings were analyzed quantitatively in conparison with the control. The analytical results of young seedlings treated with gibberellin were as follows: The stem is taller and the root is shorter than these of the control but there was not so great a difference between total weights. Also, we could not find so great a difference between moisture, ash and total nitorgen contents. But the quantity of total carbohydrate was found to be smaller, although the quantity of reducing sugar was the same. The chlorophyll content was also found to be smaller distinctly but was not so smaller as to be expected from their external appearance with much thinner and. lighter green color.View full abstractDownload PDF (190K) -
Einosuke WADA, Kyoji YAMAZAKI1950 Volume 24 Issue 8 Pages 398
Published: 1950
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (90K)
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