巻号一覧
26 巻, 10 号
選択された号の論文の14件中1～14を表示しています
- |<
- <
- 1
- >
- >|
-
中村 道徳1952 年 26 巻 10 号 p. 497-502
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーTreatment of malt extracts with 0.1% KCN at 37° for 1 hour had no effect on the α-amylase activities, but lowered the saccharogenic activities of these solutions. This treatment had marked decreasing effect on the α-amylase activities of take-diastase extracts, and on the β-amylase activities of extracts from ungerminated wheat, barley, soybean, and sweet potato, and of solutions of purified β-amylase from sweet potato. This decreasing effect wasdepenednt on the source of the enzyme as well as on the ezyme concentration in the treating mixture. Treatment of these enzyme solutions with 0.1% HCN at pH 5.0 had no decreasing effect on both α- and β-amylase activities. Potassium carbonate in 0.1% concentration showed approxinmately the same degree of inhibition on these enzyme solutions as compared with 0.1% KCN, Moreover, these 2 reagents had approximately the same raising effect on the pH values of the treating mixtures. The above results support the view, in contrast to that of ROY and UNDERKOFLER (1), that cyanide has no inhibitory effect on both α- and β-(or saccharogenic) amylase activities of the enzyme solutions used in this investigation. The apparent inhibitory effect of sodium cyanide, which they had observed in their experiments, may be the results of combined effects of pH and enzyme concentration on the pH-heat-sta-bility properties of enzyme solutions.抄録全体を表示PDF形式でダウンロード (605K) -
(第2報)甘藷葉の同化澱粉より分別したAmylopectinに就て馬場 明, 島林 幸英1952 年 26 巻 10 号 p. 502-505
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー(1) We have obtained the purified amylopectin from the assimilation starch of sweet potato leaves by the SCHOCH's butanal precipitation method and PACSU's method, and we confirmed that the amylopectin was degraded by α-amylase (crystalline malt α-amylase pr esented to us by Dr. BALLS and WALDEN) and β-amylase (crystallized from the juice of sweef potato), by using the method of potentiometric iodine titration of degraded substrate and measuring of the reducing sugar.
(2) The degree of hydrolysis of the amylopectin by β-amylase was lower than that of the amylopectin of researve starch from sweet potato tubers.抄録全体を表示PDF形式でダウンロード (401K) -
ポリガラクチュロナーゼの特異性に就て小沢 潤二郎1952 年 26 巻 10 号 p. 505-508
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー1. 酵素によるペクチンの分解物中よりdigalacturon酸及びtrigalacturon酸をブルチン塩の結晶として分離した.之等はEHRLICHのtetragalacturon酸bより低分子のペクチン分解中間物である.
2. ペクチンを分解するcarbohydraseにはpolygalacturonase Iとpolygalacturonase IIがある.前者はペクチン酸溶液の粘度を急激に下降せしめ,polygalacturon酸の鎖の中間部を分解すると考えられる.後者による場合は粘度の下降は緩徐であり,d-galacturon酸を遊離しながら分子の末端より分解するものと考えられる.
終りに御懇篤な御指導を賜つた片桐英郎先生並に北原覚雄先生に深謝する.本報告の要旨は1952年4月26日日本農芸化学会関西支部例会に於て発表した.抄録全体を表示PDF形式でダウンロード (404K) -
(第26報) Gibberellineの化學構造(その6)瀬田 靖夫, 住木 諭介1952 年 26 巻 10 号 p. 508-509
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーIn the previous paper of this series, we assumed that the position of the carbonyl group in gibberic acid should be in its side chain but not in its mother nucleus, adjusting to the terminal methyl group, from the results of the oxidation of gibberic acid with SeO2.
In this paper, the gibberic acid was treated with hydroxylamine and its oxim was prepared. This oxim was treated with phosphorus pentachloride to perform Beckmann's rearrangement and then the reaction mixture was hydrolysed with concentrated hydrochloric acid. As the hydrolytic products, the methylamine (0.7 moles), a dibasic acid, C16H18(COOH)2, (0.3 moles) and a substance, d. p. 305_??_7° (0.2 moles) were obtained. The methylamine was identified as its picrolonate and a dibasic acid was identified from the results of the elementary analysis and the molecular weight determination. The crystalline substance decomposing at 305_??_7° was assumed its molecular formula would be C18-19H21-23O3N.
From the facts above described we confirmed that the position of the ketone group in gebberic acid must be located not in the fluorene nucleus but in one of the side chains, adjusting to the methyl group of its side chain and, moreover, the terminal radical of this side chain must be a methyl group but not a carboxyl group, as it was shown in the figure.抄録全体を表示PDF形式でダウンロード (197K) -
(第2報) 3-Acetyl-4, 5-dihydroxycoumarinの合成石井 義男1952 年 26 巻 10 号 p. 510-513
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーThe author synthesized the coumarin derivatives, substituted a hydroxy radical for the carbon atom at the 5th position of 3-acetyl-4-hydroxycoumarin, to xamine the effect on the antibacterial activity of the negative substituent in benzene nucleus of the coumarin.
The schema of the synthesis was illustrated by a chart. 2, 6-Dihydroxybenzaldehyde was prepared as a needle, m. p. 116°, from resorcin, introducing an aldehyde between the two hydroxyl groups by Gatterman's reaction, acetylating these hydroxy groups, and then oxidating the aldehyde to carboxylic group. In this oxydation, the yield of diacetoxy-benzoic acid was found to be. best when three times as much of KMnO4-were used as it was theoretically calculated. 3-Acetyl-4, 5-dihydroxycoumarin, mp. 158°, was obtained in 30% of the yield, condensing 2, 6-diacetoxybenzoic acid chloride with sodium acetoacetic acid ethyl ester in the solution of the dried ether. In this case, almost no 3-acetyl-4-hydroxy-5-acetoxycoumarin was obtained.
The antibacterial activities of these derivatives of coumarin will be published in the following paper.抄録全体を表示PDF形式でダウンロード (391K) -
(第2報)細菌發育曲線の推計學的取扱い方松山 晃1952 年 26 巻 10 号 p. 513-519
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーThe growth of the bacteria (Escherichia coli) was measured using the photoelectric turbidimeter, and the fitness to logistic curve (4) introduced by VERHURST was examined. The results were as follows;
I. (i) The variable y in the equation (4) has been defined as y=Y+ε, where Y is the bacterial turbidity (log) established so that the turbidity at the time of the inoculation may be zero, and ε is L/(1+era). Y is observable but ε is unknown.
(ii) The equation (6) of finite differences derived from the equation (4) is used to examine the graphical goodness of fitting to the logistic curve (4). The points on this diagram of finite differences (Fig. 4) are on a straight line, and this fact means the graphical good fitness.
(iii) Parameters L, r and ε are calculated diretcly from the value of P1, Q1, P2, and Q2, but the estimate of ε is adopted from the value of the L/(1+era). By means of Interpolation, a is estimated.
II. According to the method of the least squares presented by W. E. DEMING, the minimum value of the sum of the weighted squares was calculated.
In order to determine the weights in y-coordinate, the standard deviations of the errors caused by incubation and measuring were estimated experimentally (Fig. 5). As the result of chi square test in which x0_??_=minS/σ_??_=49.949, degree of freedom=8, and Pr {x2≥X02}<0.001, it cannot be allowed that the growth curve of the bacteria follows the logistic curve (4).抄録全体を表示PDF形式でダウンロード (666K) -
(第9報)花崗岩の風化 (1)原田 光1952 年 26 巻 10 号 p. 519-523
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー(1) The weathering of granites on the, outskirts of Tottori City, at t ukuwatari-macni, Okayama-ken, and at Tamayu-mura, Shimane-ken was studied. (Annual mean temperature 14.5_??_15.0°, annual rainfall 1200_??_1900min) (2) The granites decompose gradually, and the light yellowish-brown coloured sandy under-soils and the brown coloured sandy surface soils are, formed. (3) The NIGGLI's h values increase with the progress of weathering, but decrease somewhat in the surface soils, as compared with the under soils. (4) The si/al values decrease with the progress of weathering. In some cases, however, these values are less in the under soils than in the surface soils, and it indicates that the clay having the low si/al values has been transferred from the surface horizon to the under horizon. (5) More Na is leached away than K, and more Ca is removed than Mg. Among the bases, Mg is the least.Jiable to be leached out. (6), With the progress of weathering, there are the increases of complex A and complex B. In some cases, however, the complex A decreases in surface soils, as compared with the under soils. (7) The weathering products contain a small amount of silica and alumina which are soluble in alkali-solutions.抄録全体を表示PDF形式でダウンロード (506K) -
(第5報)鰯油及び鳥賊内臓油のBOUVEAULT-BLANC還元及び尿素法によるオレイルアルコールの製取に就て益山 新六1952 年 26 巻 10 号 p. 524-527
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー(1) Unsaturated long-chain fatty alcohols were prepared in good yield by the reduction of sardine and calamary oils with sodium and ethylene glycol monoethyl ether (Cellosolve) in toluene or xylene.
(2) These alcohols were fractionated by vacuum distillation and the iodine values of the fractions were observed.
(3) A new method of fractionating long-chain fatty alcohols on the basis of their degree of unsaturation has been found iri extractive crystallization with urea. Separations of sardine oil alcohol and calamary oil alcohol are shown, and the preparation of oleyl alcohol by this method is outlined.抄録全体を表示PDF形式でダウンロード (406K) -
(第4報)螢光菌及び霊菌によるα-Ketoglutaric及びPyruvic Acidの生成に就て朝井 勇宣, 相田 浩1952 年 26 巻 10 号 p. 528-533
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーThe production of α-ketoglutaric acid by Pseudomonas fluorescens has been described by LOCKWOOD and STODOLA (1946). By shaking culture we also confirmed that Pseudomonas 33 F also produced this acid and further newly found that Serratia marcescens No. 18 also produced this acid via 2-ketogluconic acid. From 7.64% glucose solution containing 0.1% ammonium sulphate and other mineral salts, Pseudomonas 33 F and Serratia marcescens No. 18 produced 1384mg and 1899mg of α-ketoglutaric acid per 100cc of culture fluid, respectively.
The identification of α-ketoglutaric acid was conducted as its Ca-salt, 2, 4-dinitrophenylhydrazone and by the paper chromatographic method. The crystal of free acid was also acquired.抄録全体を表示PDF形式でダウンロード (612K) -
河野 哲郎1952 年 26 巻 10 号 p. 533-536
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー(1) A high frequency titration apparatus was used to study the action of urease upon urea, and the reaction processes were plotted precisely from the very beginning of the reaction.
(2) The facts formerly known, the activation of a crude urease by glycine, and the inactivation by means of Ag+ or diazobenzenesulphonic acid, were confirmed.
(3) When titrated with 0.01 N HCl or H2SO4, the enzymatic product soln. gave a threestepped curve. A soln. of (NH4), CO3 and enzyme gave the same one, but that of (NH4)HCO3 and enzyme gave a two-stepped one.
(4) The method was examined quantitatively, and was found to be almost satisfactory. It can be used, of course, for the quantitative analysis of urea, with the help of urease.抄録全体を表示PDF形式でダウンロード (292K) -
(第6報) β-Metyl-cellobioside (acetate)のα-fromへの転換井上 吉之, 小野寺 幸之進, 唐澤 郁夫, 西澤 嘉彦1952 年 26 巻 10 号 p. 537-539
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーThe transformation of beta methylcellobioside heptaacetate into its alpha isomer, by the action of titanium tetrachloride, antimony pentachloride and selenium tetrachloride, was studied.
A crystalline substance, obtained as the transformed product from the reaction mixture in all of three cases mentioned above, has the melting point of 175° and the [α]D of +24.5°, and seems to be identical with the second crystalline substance described by PACSU(5), while the crystals, melting at 185° and having [α]D=+55.7°, were not found in the reaction product of our repeated experiments.
It is probable that the crystalline substance obtained (m. p. 175°; [α]D+24.5°) is alpha methyl cellobioside heptaacetate despite of the disagreement of the rotation with the value calculated by the HUDSON's second rule of isorotation. Further invetigation is being conducted.抄録全体を表示PDF形式でダウンロード (302K) -
中林 敏郎1952 年 26 巻 10 号 p. 539-541
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーAs the existence of monoglucoside of flavonoid pigment in the flower of Astragalus sinicus. L. was supposed after the study by the method of paper chromatography, I have studied its isolation and analysis. From the ethyl acetate extract, yellow needle crystals (m. p.178°C) were isolated. From the results of the qualitative and quantitative analyses, I have concluded that the structure of this pigment is kaempférol-3-glucoside and it is a new glucoside of kaempferol. So I have named this flavonoid pigment “astragalin”.抄録全体を表示PDF形式でダウンロード (293K) -
(第2報) Lactobacillus caseiによる滴定法の檢討,並に本法の消化管内容物及び糞への適用に就て吉原 一郎1952 年 26 巻 10 号 p. 542-547
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーE. E. SNELLの原報記載の培養基から出発して,定量の為の基礎的事項及び消化管内容物及び糞への適用について検討し次の結果を得た.
1. Flavinの量により異るが. L. caseiの酸生成は葡萄塘又はペルトン液とシスチンの増加により,いずれも増加し,無機塩の増加により減少する.且葡萄糖の増加はflavin量と酸生成量との間に,より完全な比例関係を成立せしめるのに有効なるものの如くである.
2. 液層の厚さ及び接瞳菌数の差により,酸生成量が異るとは認められない.
3. 試料の抽出液中のflavinは1γ/10cc以下の濃度で, pH9.0にて100watt. 72時間の照射で完全に破壊される.
. 4. 兎の糞及び小腸内容物中には,flavin以外に酸生成に影響する物質は先ずないと考えてよく,従つて本法を以て定量することが出来る.
5. 兎の盲腸,山羊の第一胃内容物中には, flavin以外に酸生成に影響する物質があり,之らの試料には本法をそのまま適用することは危険であり,尚検討を要する.
終りに実験に当り終始御懇篤な御指導と御鞭燵をいただいた神立助教授,データの整理に当つて御助力と御教示をいただいた統計数理研究所遠藤氏,本学講師増山氏に厚く感謝の意を表する.本研究は文部省科学研究費によつて行つた.抄録全体を表示PDF形式でダウンロード (655K) -
(第2報)麹酸生成否による分類(第1報補遺)小原 巖1952 年 26 巻 10 号 p. 547-551
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーIn the preceding paper, the author introduced an analytical key to the Aspergillus tamariioryae group and it was stated that the ability to produce kojic acid from various compounds in the chemically defined medium could aid species and varieties identificaticn which was difficult when based on the evaluation of the cultural and morphological characteristics.
In this paper, investigations have been made on more forty strains (Table la, b) and some changes made in the key which should include all the strains of yellowish green to brownish Aspergilli.抄録全体を表示PDF形式でダウンロード (420K)
- |<
- <
- 1
- >
- >|