巻号一覧
26 巻, 6 号
選択された号の論文の13件中1～13を表示しています
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(第2報) I. 子宮收縮性物質に就て (ii)里村 幸男1952 年 26 巻 6 号 p. 277-279
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーPhysiological action of the alcoholic extracts of sclerotia and mycelial mass formed by the culture of Scl, Lib. on grain byproducts (rice-bran, ctc.), after being evaporated to small vol. and treated by a proper process, was tested pn cock's comb, frog's pupil, frog's vessel and rabbit uterus.
It was found that the extracts like ergot's alkaloid suppressed the motor action of adrenaline on the iris muscle or blood vessel of frog, and uterus-active constituent was somewhat rich in the mycelial mass formed by a mutant Sm, rather than in mycelial mass, which contains sclerotia, formed by the original strain Ss.抄録全体を表示PDF形式でダウンロード (349K) -
(第3報)自然變異の原因に關する研究(ヘテロカリオシス)坂口 謹一郎, 石谷 千代子1952 年 26 巻 6 号 p. 279-285
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー1. 麹菌の変異株間(Asp. oryzae, aXから生じたS及びF-type)及び異株間(Asp. oryzae var. magnasporus A5-3とS-type)にはanastomosisが見られる.
2. Anastomosisをした菌糸間及び発芽管の間のfusion-bridgeに於いては核分裂が盛んで膜を通して核の移動がみられる.
3. 固定した変異株間(S×F)の混合培養によつて中間株(X-type)を生じ,このX-typeのpedigree-cul-tureによつて再びparentのS及びF-typeとX-typeを分離する.
4. 上記のS及びF-typeはconidiaの直径比較的齊一で核の数は1～4であるに反し,このX-typeは明にconidiaの直径が不揃いで大きいものを含み,4～8又はそれ以上の多核性の核を含む.
5. 培養麹菌の異株間(Asp. oryzae 10株及びAsp. sojae 2株の種々の組合せ)のmix-cultureによつて,両親と異つた中間雑株を生じる.
終りにのぞみ有益な御助言にあづかつている和田教授に謹んで謝意を表する.抄録全体を表示PDF形式でダウンロード (2227K) -
サルファイト蒸解の研究第3報軒原 栄三, 田中 竜三, 大江 礼三郎1952 年 26 巻 6 号 p. 286-289
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーExtracted spruce wood meal, spruce holocellulose and rayon pulp were cooked with NH2OH•HCl solution in ethanol (pH 2.5) in the boiling water bath during 1_??_20 hrs. Acetal linkages in these materials are hydrolyzed and the liberated carbonyl groups make oxime with the production of equivalent HCl. The curves (produced HCl-reaction time) for holocellulose and rayon pulp were linear, whereas in the case of spruce wood the rate of HCl production was very rapid in the earlier stage and the curve turned to straight line after about 8 hours. Extraporating the latter to time zero, it was concluded that 1g wood meal liberated 0.3 m-mol HCl within a few earlier hours. The present authors suppose that this 0.3 m-mol HCl per g wood must be produced by cleavage of acetal linkages between carbonyl group of lignin and carbohydrates, and therefore lignin has 1 mole carbonyl group per 960g.抄録全体を表示PDF形式でダウンロード (433K) -
(第2報)被害部變色に關係ある二,三の要因に就て瓜谷 郁三, 村松 敬一郎1952 年 26 巻 6 号 p. 289-295
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーThe plant tissues usually turn brown, when they are affected by pathogenic microorganisms: Many papers have been published about this problem, but no theory supported by chemical experiments has been established. We have selected the sweet potato infected with Ceratos, tomella fimbriata, so-called “black-rotted” sweet potato, to carry out our experiments.
In this study, the authors examined, from the point of the view mentioned above, the rise and fall of activities of some enzymes and polyphenols which were contained in the injured parts as well as in the sound parts in order to observe the abnormal metabolism caused by the infection. The results summerised are as follows.
The determination methods of the activities of oxidase, peroxidase, and catalase, and the quantity of polyphenols were improved so as to be suitable for our experiments.
It was ascertained that, using the uninoculated sound potato as the control, the oxidase and peroxidase were highly activated and polyphenols were noticeably accumulated in the sound part adjacent to the injured. This abnormal metabolic phenomena were observed more distinctly. in case of sufficient supply of oxygen than in case of lack of oxygen, and more in case of Vari-ety Norin No. 1 than in case of Variety Okinawa No. 100.
As to the reason why the injured part turned brown, it was ascertained that ate first the function of the protoplasm was destroyed and then the oxidation of polyphenols by activated`.oxidase and peroxidase, occurred 'irreversively, and the quinoid substances thus formed were polymerized immediately, as, soon as the cells which were working out the abnormal metabo-lisrn mentioned above were penetrated by this fungus.
The oxxidase and peroxidase of this fungus had some effects on the browning of the tissue though the enzyme activities of-this fungus were weak.
It was observed that the polyphenolic and quinoid substances might inhibit the penetration of the fungus into the cells cooperating with the bitter substances and resins contained in the injured part. The details including the inhibitory activity, of these substances will be published in Part V of this series.
Higher activities of catalase in the injured part was found to be due to the presence of cata-:las, e in the--mycellium of this fungus grown in the injured part.抄録全体を表示PDF形式でダウンロード (759K) -
(第2報)その形成に金屬イオンが關與せざる酵素系の形成,作用に及ぼすFusarinic acidの影響玉利 勤治郎, 加治 順1952 年 26 巻 6 号 p. 295-298
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーFusarinic acidはその形成に金属イオンが関与しない酵素の形成には何等阻害作用を示さない専を明かにした.尚fusarinic acidがα-β不飽和ケトン等の場合に見られる如く-SH基を阻害する能力があるのでないかとの懸念からcysteineとの拮抗作用を試験したのであるが結果は否定的であつた.抄録全体を表示PDF形式でダウンロード (412K) -
(第3報) Fusarinic Acidと構造類似化合物の水稻生育に對する阻害作用の比較及び考察玉利 勤治郎, 加治 順1952 年 26 巻 6 号 p. 298-303
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーFusarinic acidと構造類似の各種化合物こ就て試験せる結果はchelate ring.を形成する能力ある物質は水稲種子の発芽生育阻害作用も顕著であるが,然らざる物質では阻害作用は殆ど認められなかつた.又chelationの強い物質程阻害作用も強く両者の間には極めて深い関連性がある.尚fusarinic aoidの阻害作用が分子内錯塩の形成にある事を裏書きする事実として,そのcarboxyl基をエステル化する事に依つてその阻害作用が著しく減弱する事が認められる.
本研究を遂行するに当つて御指導と御力添えを頂いた恩師東大名誉教授藪田貞治郎博士,東大教授住木諭介博士及び試験籾を分与して頂いた新潟県長岡農事試験場の各位に厚く謝意を表する.又その研究費の大部分を文部省自然科学研究費に仰いだ事を附記して茲に深く感謝する.抄録全体を表示PDF形式でダウンロード (1852K) -
サルファイト蒸解の研究第4報軒原 榮三1952 年 26 巻 6 号 p. 303-306
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーOne hundred g of, the extraction residue of spruce wood meal (with EtOH-C_??_H3and hot water) was refluxed with the solution, which is composed of 1000 cc EtOH, 67.7g conc. HCl, 21g NH2OH•HCl and 60 cc water, on the water bath during 20 hours. About 40% of lignin in the wood was removed in this process. When the filtrate and washings were poured into 5l H2O, brownish yellow flocculent amorphous substance (α) was precipitated (5.7g). After (α) was filtered off, the filtrate was neutralized to pH 3.4, concentrated in vacuo at 55° and treated continuously with ether. Ether extraction after concentration left yellow syrup (β) (1.65g). From the determinations of OCH3 and N contents of (α) and (β), it was concluded that (α) was lignoxime, having one N (i.e. one mole carbonyl group) vs. 3.5 lignin building stones, and (β) was low-molecular lignoxime, having one N vs. one lignin building stone.抄録全体を表示PDF形式でダウンロード (388K) -
(第3報)加熱後の培養基組成の影響(その一)特に糖類の效果に就て天羽 幹夫1952 年 26 巻 6 号 p. 306-313
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー(1) The influences of subculture media on the survival times of spores were studied. The, spores of Bacillus natto were suspended in neutral phosphate buffer and heated at. 100°C, and. then subcultured by various media. The yeast-extract broth (10% bakers' yeast extracted cted by nutrient both, adjusted to pH 7.0). liver-extract broth (10% pork-liver extracted by nutrient broth), and glucose-broth (1% glucose added to nutrient broth), showed survival time-twice as long as plain nutrient broth. The addition of reducing agents, cysteine and thioglycollate to nutrient broth, have shown no increasing effect on the survival time. (Table 1)
(2) The effects of adding various carbohydrates and organic acids were examined. Fructose, mannose, galactose, sucrose, maltose, and soluble starch were found to have the increasing effect on the survival time of spores of Bacillus natto to the same extent as glucose, but other eight sugars, arabinose, xylose, lactose, trehalose, mannitol, glycerol glycogen, and Cori-ester have had no effects.
The addition of pyruvate or alpha-glycerophosphate to nufttent broth also increased the, survival time, but the addition of organic acids such as lactic, acetic, and succinic had noeffects.
It was found that in a synthetic medium(9), which is composed of eighteen amino acids, nine vitamins, four purine-pyrimidine bases, and salts, the vegetative cellls of Bac. natto grew well without the addition of glucose, whereas the spores could not grow without glucose (carbohydrates). And it was also found that the kind of carbohydrates which were effective in increasing the survival time of the spores of Bacillus natto was the same with that which served, as the energy source and which enabled the spores to grow in the synthetic medium. (see Table 3 and 5). The minimum effective, concentration of glucose in increasing the survival time of the spores was between 10-5M and 10-6M, and the same order of glucose concentration also has limited the growth of spores of the strain in the synthetic medium (see Table 4 and 5).
(4) It should be noted that when subcultured by glucose broth, the survival time at each spore concentration was commonly about twice that of plain nutrient broth, throughout a wide range of spore concentration. The relation between spore concentration (N) and survival time (t) in the case of subculturing by glucose broth, can be expressed by the following general formula as reported in the previous paper(7). logN=a+blogt
(5) The discussions on the mechanisms of spore survival time increasing activities of glucose were delivered.抄録全体を表示PDF形式でダウンロード (827K) -
(第2報) Pseudomonas屬酸化細菌の生化學的研究横沢 清1952 年 26 巻 6 号 p. 313-318
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリー1. The biochemical properties of thirteen strains of the genus Pseudomonas isolated by the author were studied.
2. These strains were very weak in their oxidative abilities for ethanol.
3. These strains oxidize glucose to gluconic acid or to 2-keto-gluconic acid. In the case of shaking culture, glucose was completely oxidized in 48 to 72 hours. Five strains of them produced only gluconic acid and eight strains produced only 2-keto-glueonic acid from glucose and the Tatters oxidized gluconic acid to 2-keto-gluconic acid.
4. These strains did not oxidize sorbitol, mannitol or glycerol.
5. These strains did not oxidize maltose or lactose.抄録全体を表示PDF形式でダウンロード (551K) -
(第3報) I. 子宮收縮性物質に就て (iii)里村 幸男1952 年 26 巻 6 号 p. 318-320
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーPharmacological study on the extrats of mycelial mass formed by the culture of Sclerotinia Libertiana was performed at Pharmacological, Institute of Medical Faculty, , Kyushu University, and it was found that uterus-contracting action of the extract against rabbit uterus, both in situ and isolated, is comparable to “gynergen”.
An antagonistic substance against adrenaline disappeared in a process of preparation of the extract.
Clinical application performed at Obstetrical Institute of Medical Faculty, Kyushu University, showed good results, with only small side reactions.抄録全体を表示PDF形式でダウンロード (875K) -
(第4報) I. 子宮收縮性物質に就て (iv)里村 幸男1952 年 26 巻 6 号 p. 320-323
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーThe uterus-contracting constituent of Sclerotinia fungus extract was ascertained not to be alkaloid or biogenic amine.
Most part of the active fraction was precipitated by alcohol (60_??_95%), and the remaining liquid had a small effect.
The active fraction, which contains N and has a glycosidelike nature, lost the activity after acid hydrolysis, but it did not change after autolyzation of maceration juice.抄録全体を表示PDF形式でダウンロード (411K) -
(第1報補遺)培養條件並びに純粹分離に對する知見(其の2)山田 浩一, 藤本 義典, 船田 讓1952 年 26 巻 6 号 p. 323-325
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーTo get a pure culture of cellulose thermophiles, the agar plating method was examined using Na carboxylmethylcellulose, cellodextrin or cellobiose as the carbon source under a suitable, rH and an anaerobical condition with carbon dioxide or nitrogen gas. By this method nearly pure cultures of cellulose thermophiles were obtained, but these failed to digest cellulose and to recover their activity. The filtration method was also unsuccessful, because their sizes were not remarkably different and a homogeneous culture was not obtained owing to their short reproduction time.抄録全体を表示PDF形式でダウンロード (297K) -
佳木 諭介, 山本 勘二, 武田 強二1952 年 26 巻 6 号 p. 325-328
発行日: 1952年
公開日: 2008/11/21
ジャーナル フリーWe have synthesized the following quaternary ammonium compounds. (1-a) dimethyl-octyl-benzyl ammonium chloride, mp. 74°, plates ; (1-b) dimethyl-dodecyl-benzyl ammonium chloride, mp. 45°, scales; (1-c) dimethyl-hexadecyl-benzyl ammonium chloride, mp. 55_??_6°, needles; (1-d) dimethyl-octadecyl-benzyl ammonium chloride, mp. 150_??_8°, scales; (2) dimethyl-dodecyl-methylthioethyl ammonium. chloride, mp. 59°, plates; (3) N, N'-bisdimethyl-N, N, N'-bisdodecyl-(N-ethyl-thio-N'-ethyl)-diammonium chloride, mp. 209°, scales; (4) dimethyldodecyl-phenylthi-oethyl ammonium chloride, mp. 82.5°, needles; (5) nicotine-N, N'-bisdodecyl chloride, mp. 99°, scales.
We have examined their germicidal activities using the spores of Gibberella Fujikurol as testing microorganism and compared with that of a 10-3 solution of Uspulum (Uspulum contains 3% of methoxyethyl mercuric chloride and, therefore, the concentration of this Uspulum solution corresponds to a 3×10-4 solution of methoxyethyl mercuric chloride). The figures in the following table indicate the dilution of synthesized compounds which shows the same physiological activity as that of a 10-3 solution of Uspulum.
(1-a) 100 (1-c) 160.000 (2) 10.000 (4) 220.000
(1-b) 800.000 (1-d) 120.000 (3) 80.000 (5) 4.000抄録全体を表示PDF形式でダウンロード (338K)
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