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Volume 40, Issue 6
Displaying 1-5 of 5 articles from this issue
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The Effects of Low Protein and Low Caloric Diet on the Urinary Nitrogenous and Liver Components of Young RatsHiroo YOKOTSUKA, Kyoko KOMINE, Hiroshi NISHI1966 Volume 40 Issue 6 Pages 241-245
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe young rats were fed for 60 days on diets containing 7.5% casein at three levels of caloric intake; 100, 80 and 60% of full caloric requirement. Nitrogen balance, urinary nitrogenous and liver components were determined after 30 and 60 days from initial feeding of experimental diets. (1) The growth after 60 days was proportional to the level of caloric intake. Apparent digestibility of protein and apparent retention
Ingested N-(Fecal N+Urinary N)/Absorbed N×100
were unaffected by the caloric intake levels except some detailed points. (2) Each of the percentages of urinary amino, allantoin and creatinine nitrogens to total nitrogen among each group was compared. It was found from the data after 30 days that these values were decreased by caloric restriction. These data show the tendency that the characteristic pattern of urinary nitrogenous components of rats fed on low protein diet is lost by caloric restriction. (3) Glycogen content in dried liver was decreased and inversely protein content was increased by caloric restriction. The deposition of more than 20% of liver fat was found in all groups, and remarkable effect of caloric intake levels on the fat deposition was not found from the data after 30 days. It was found that the moisture percentages of wet liver of rats after feeding for 60 days were increased by caloric restriction.View full abstractDownload PDF (439K) -
Phenolic Substances of Red Clover Leaves and Effects of p-Coumaric, Caffeic and Chlorogenic Acids on the Digestibility of the Leaf ProteinsTakao HORIGOME, Makoto KANDATSU1966 Volume 40 Issue 6 Pages 246-251
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSOn paper chromatogram of phenolic substances in red clover leaves, three major components were found, besides free p-coumaric and caffeic acids. One of them was similar to chlorogenic acid in its colour reactions and UV-absorption spectra, although there was some doubt compared with authenic specimens of chlorogenic and isochlorogenic acids in RF value, and the second to p-coumaroylquinic acid. The third component, which probably reacted in the browning of red clover leaves, was not identified.
The effects of p-coumaric, caffeic and chlorogenic acids on digestion of leaf protein were studied with pepsin. Since the acetone powder of fresh leaves of red clover contained about 34% of protein and showed polyphenoloxidase activity, it was used as protein substrate. The digestibility of the leaf protein decreased when the acetone powder was incubated with buffer solution (pH: 3_??_8) and phenolic acid at 37°C for 3 hrs. before proteolytic digestion. Among three acids, caffeic acid was most effective.View full abstractDownload PDF (449K) -
Isolatio of the Protease-Producing Bacteria and their Bacteriological CharactersYûji FURUKAWA, Yoshitsugu FUJII, Teijirô UEMURA1966 Volume 40 Issue 6 Pages 252-256
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThirty-two strains of aerobic bacteria, isolated from eighty-seven soil samples, were tested for the protease-producing abilities. It was found that considerable differences existed in their capacities in producing D-collagenase, gelatinase and caseinase. Among three strains, which showed higher D-collagenase activity, strain Kp-931 was the strongest D-collagenase producer and thus selected as the test organism. The crude protease preparation obtained from the culture filtrate of this organism had pH optima at 8.0 to 10.0 with hide powder, at 9.0 with gelatin and at 8.0 with casein substrates. From further experiments on the protease produced into the medium at different phases of cell growth, it was observed that the maximum D-collagenase activity was found at the later stage of logarithmic growth phase while the maximum gelatinase activity was reached at the stationary phase. These results suggest that a specific enzyme which digests hide powder is produced by this aerobic organism. The strain Kp-931 was identified as Bacillus cereus from morphological and physiological studies.View full abstractDownload PDF (412K) -
Effects of Sodium Salt of Several Bile Acids on L- Glutamic Acid Formation under Excess Amount of d-BiotinTeruo HATTORI1966 Volume 40 Issue 6 Pages 257-260
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIt is well-known result that addition of several antibiotics or surface-active agents into fermenting broth causes good production of L-glutamic acid under excess amount of biotin. The author also tested the effect of sodium salts of several bile acids, surfaceactive agents, by using Micrococcus glutamicus ATCC 13058 on synthetic medium in which excess biotin was contained. As the result of the test, it was found that deoxycholate was most effective for L-glutamic acid production, chenodeoxycholate was second, and other bile salts, namely, cholate, taurocholate and glycocholate, were almost not effective.View full abstractDownload PDF (282K) -
Masaaki HORIGUCHI1966 Volume 40 Issue 6 Pages R25-R30
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (555K)
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