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Volume 48, Issue 9
Displaying 1-9 of 9 articles from this issue
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Teh-Chou CHANG, Itsuo ICHIMOTO, Hiroo UEDA1974 Volume 48 Issue 9 Pages 477-481
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe synthetic method of β-ketosulfoxides from aliphatic δ-ketoesters was studied. Methyl 2-alkyl-5, 5-ethylenedioxyhexanoates (R=H, 10 a; R=CH3, 10 b; R=isopropyl, 10 c) were treated with methyl sulfinyl carbanion to afford the corresponding β-ketosulf oxides (11 a_??_c) . The resulting 6, 6-ethylenedioxy-3-isopropyl-1-methylsulfinyl-2-heptanone (11 c) was reduced by aluminum-amalgam to give after acid-catalyzed hydrolysis 3-isopropyl-2, 6-heptadione (1). Under both acidic and basic conditions, 1 was cyclized to form piperitone (2) predominantly.View full abstractDownload PDF (321K) -
Tatsuo FUJIKAWA, Michio SHIOMI, Sunao MUKAI, Masafuto WADA1974 Volume 48 Issue 9 Pages 483-491
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDubois's phenol-sulfuric acid method for the colorimetric determination of sugar was mainly investigated concerning the influences of impurity. Comparison of recovery of lactose from its solutions (50 μg/ml) containing impurity was as follows.
1) The recovery was not disturbed much by Na+, K+, Mg2+ (as sulfate, 0.01M), Ca2+, Ba2+ (as chloride, 0.01M), Cl-, Br-, SO32-, PO43- (as Na-salt, 0.01M), acetic acid, succinic acid (0.1M), or oxalic acid (0.02M).
2) NO2-, NO3- (as Na-salt), I- (as K-salt), lactic acid, or tartaric acid disturbed strongly the recovery as a result of coloring caused by them. Cu2+, Al3+, Fe2+ (as sulfate), borate (as Na-salt), citric acid, or malic acid also exerted a considerable influence though no or few colorings were caused by them. Their maximum limits allowed were Fe2+, Al3+, borate; 0.001, Cu2+, NO3-, I-; 1×10-4, NO2-; 1×10-5, lactic acid; under 0.001, tartaric acid; 2×10-4, citric acid; 0.02, and malic acid; 0.002M.
3) The disturbances by these organic acids except lactic acid could be, however, removed by means of precipitation of these acids as Ca-salt.
4) The recovery was not exerted by metaphosphoric acid, methanol, and chloroform used as deproteinizing agent. CdSO4, HgSO4, basic lead acetate, and ethanol could not be used without exact removal of them.View full abstractDownload PDF (597K) -
Masaaki SUGIURA, Toshio SHINBO, Masayoshi KIKKAWA, Harukazu TOYODA1974 Volume 48 Issue 9 Pages 493-498
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe regenerated collagen membranes obtained from a bovine hide were treated with formaldehyde solutions, and the membrane potential and the ionic permeability of these membranes for potassium and sodium chlorides were measured. From the results obtained, the effect of cross-linking with formaldehyde on the ion permeation through the membrane was discussed. The concentrations of formaldehyde solutions used for the hardening of the collagen membranes were 0.05, 0.5 and 2.5%. The amounts of formaldehyde combined with the membranes were 0.03, 0.25 and 0.32mM/g respectively.
The membrane potential increases with an increase in the amount of combined formaldehyde, and the curve of membrane potential against pH for potassium chloride is shifted to lower pH regions: it is found from this curve that the isoelectric points of the collagen membranes treated with 0.05, 0.5 and 2.5% formaldehyde solutions are pH's 4.95, 4.8 and 4.7 respectively. The ionic permeability of formaldehyde tanned membranes becomes minimum at the isoelectric point of the membranes. However, the change in the permeability with pH decreases with an increase in the cross-linkage with formaldehyde: the permeability increases in the vicinity of the isoelectric point, while it decreases in the other regions.View full abstractDownload PDF (403K) -
Kazuki SHINOHARA, Yuji FUKUMOTO, Yaw-Kun TSENG, Yuzuru INOUE, Hirohisa ...1974 Volume 48 Issue 9 Pages 499-506
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAmino reductones (TR-AA) were prepared by the condensation reaction of triose reductone with some amino acids such as leucine, methionine and phenylalanine and their action on nucleic acids was investigated.
It was confirmed that amino reductones brought about double strand and single strand scissions of native DNA. Furthermore, they depolymerized heat-denatured DNA and RNA, too. Their action was remarkably accelerated with Cu2+. Triose reductone cleaved also nucleic acids.
From UV spectra, paper chromatogram and elemental analysis, it was found that TR-AA were rapidly oxidized and decomposed to mesoxal-dialdehyde and amino acids under the co-existence of Cu2+ or p-quinone.
TR-AA pretreated with Cu2+ or p-quinone for 5min had not DNA-splitting activity, and DNA scission with TR-AA-Cu2+ system was caused together with the rapid oxidation of TR-AA in a few minutes. However, degradation of DNA was not observed in TR-AA-p-quinone system. It was therefore suggested that the oxidation process of TR-AA with Cu2+ which resulted in DNA scission was different from that with p-quinone.View full abstractDownload PDF (514K) -
Yokichi MURAMOTO, Koji OISHI, Itsuo ICHIMOTO, Hiroo UEDA1974 Volume 48 Issue 9 Pages 507-513
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe selective alkylation of ethyl 3-oxoglutarate was achieved through its Mg-complex prepared from a normal coordinate treatment with Mg (OEt)2. The structural elucidation of this complex was substantiated by comparison of Cu and Mg chelate compounds prepared from acetylacetone and ethyl acetoacetate.
Mg-complex of ethyl 3-oxoglutarate reacted with alkyl halides (RX) in the presence of Mg (OEt)2 or NaOEt as basic catalyst, followed by hydrolysis with hydrochloric acid to give ethyl 2-R-3-oxoglutarate (yield, 78_??_80%). Moreover, the synthesis of ethyl 2-R-4-R'-3-oxoglutarate from ethyl 2-R-3-oxoglutarate can be conducted successfully by using alkyl halides (R'X) in the presence of NaOEt as catalyst (yield, 81_??_82%).View full abstractDownload PDF (485K) -
Toshiro SHIDA, Yasuo HOMMA, Tomomasa MISATO1974 Volume 48 Issue 9 Pages 515-519
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSRice blast disease-preventive activities of seventy-seven amino acid derivatives were examined by using rice seedlings of the 4- or 5-leaf stage. Among them, N-phenacetyl-L-tryptophan, N-naphtyl-L-leucine, N-naphtyl-L-valine, N-propyonyl-L-isoleucine and several N-amino acid esters such as lauryl DL-valinate HC1 and lauryl-DL-alaninate HCl showed the most constant activity. Amino acid esters which had preventive activities for rice blast had strongly inhibitory effect on spore germination of Pyricularia oryzae Cavana, while N-naphtyl-L-valine, N-naphtyl-L-leucine, N-phenacetyl-L-tryptophan and N-propyonyl-L-isoleucine had no effect. The relationship between preventive values and permeation of N-glycine fatty acid ester, DL-alanine fatty acid ester and DL-threonine fatty acid ester was discussed.View full abstractDownload PDF (376K) -
Takanori KASAI, Sadao SAKAMURA1974 Volume 48 Issue 9 Pages 521-523
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSInfrared spectra of ten pairs of α- and γ-glutamyl dipeptides and several related compounds were measured in KBr disks. The amino acids which constituted the peptides were all L-form. Carbonyl stretching vibrations of α-glutamyl peptide bonds (1685_??_1665cm-1) appeared at higher wave number than those of γ-glutamyl peptide bonds (1660_??_1635cm-1), but NH stretching vibration of α-glutamyl bond was observed at lower wave number than that of corresponding γ-glutamyl bond.View full abstractDownload PDF (186K) -
Yokichi MURAMOTO, Itsuo ICHIMOTO, Hiroo UEDA1974 Volume 48 Issue 9 Pages 525-527
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSMethanolysis and ethanolysis of ε-caprolactone, δ-valerolactone and γ-butyrolactone in the presence of magnesium alkoxide as catalyst were studied. ε-Caprolactone and δ-valerolactone were converted quantitatively to the corresponding ω-hydroxyesters, which could be isolated in the yield of 85_??_90%. However, conversion of γ-butyrolactone was less than 60%. The comparison of these results with those of acid-catalyzed alcoholysis using sulfuric acid and Amberlite IR-120 is also discussed.View full abstractDownload PDF (214K) -
1974 Volume 48 Issue 9 Pages N131
Published: 1974
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (26K)
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