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Volume 49, Issue 12
Displaying 1-11 of 11 articles from this issue
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RYUZO NISHIYAMA, Michio KOZAKI1975 Volume 49 Issue 12 Pages 629-633
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSInhibitory patterns of green tea using Tamura & Tsunoda's improved medium and GYP medium for the growth of Leuconostoc mesenteroides P-60 were resembled to those of tannic acid, and the four polyphenols, (-)-epigallocatechin (EGC), (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG) and (-)-epicatechin (EC) isolated from green tea were found to be inhibitory for the growth of the lactic acid bacteria.View full abstractDownload PDF (313K) -
Yoshihide SHIMABAYASHI, Takashi MATSUMOTO1975 Volume 49 Issue 12 Pages 635-640
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSVitamin B12 binding protein was extracted with cold perchloric acid from 13 day old chick embryos. The protein was purified by successive gel filtration on Sephadexes and finally by chromatography on DEAE-cellulose.
The purified protein was shown to be homogeneous by electrophoresis on polyacrylamide gel. The molecular weight of this protein, determined by gel filtration, was approximately 105, 000.
From the extract of the protein with acetate buffer (pH 4.6) containing potassium cyanide, vitamin B12 was identified by paper chromatography and microbiological assay with Lactobacillus leichmannii. The vitamin B12 binding protein was recognized to be an acid protein from the results of isoelectric focusing and of amino acid analysis.View full abstractDownload PDF (434K) -
Isao HAYAKAWA, Seiji KAWASAKI, Danji NOMURA1975 Volume 49 Issue 12 Pages 641-646
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe relationship between the spinnability of yeast protein and viscoelastic properties was studied in comparion with commercial casein and soya protein, and following results were obtained.
1) Yeast protein which was prepared from baker's yeast by the mechanical method, called Impact-Cell-Mill method, was inferior to commercial casein, but same as soya protein in the spinnability.
This difference was derived from the purities, but not from the differences of amino acid among these materials.
2) Some relationships were confirmed in the spinnabilities and the viscoelastic properties of dopes.
Dynamic modulus (G') increased in a limited range of the oscilation speed (ω) -2<log(ω)<1, and the spinnability of these dopes were classified by the gradient values (_??_=dG'/d log(ω)).
Some differences in the shift factor (aT) were caused by the conditions of producing the dope. This effect was less in casein and much in soya protein, aT of yeast protein was ranged between those of the dopes.
3) A relaxation spectrum of ideal dope was a box type. The best spinning dope showed an ideal box type in the spectrum. Therefore, the condition of an ideal dope is considered as a simple dispersion.View full abstractDownload PDF (460K) -
Yasunobu SUKETA, Yoshio MASUI, Shuji HAYAKAWA, Yoko SUGENO, Takeo YAMA ...1975 Volume 49 Issue 12 Pages 647-652
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) The respiratory quotient of leaves of gladiolus (Gladiolus gandavensis), iris (Iris hollandica) and crocus (Crocus vernus) was decreased by HF-fumigation.
(2) The acid phosphatase activities in these plant leaves were decreased by HF-fumigation, and the sensitivity was in the following order: gladiolus>iris>crocus.
(3) The regression Y=0.897 X+0.003 of respiratory quotient (X) on the acid phosphatase activity (Y) was obtained from the data and was found to have high regression coeficient of 0.983.
(4) The oxygen uptake in gladiolus leaves was significantly increased by HF-fumigation in light period, but was slightly decreased by HF-fumigation in dark period.
(5) The protein content in gladiolus leaves was highly decreased by HF-fumigation in light period, but was not influenced by HF-fumigation in dark period.View full abstractDownload PDF (406K) -
Masato TAMARI, Masaaki HORIGUCHI, Makoto KANDATSU1975 Volume 49 Issue 12 Pages 653-659
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSWe tried to isolate microorganisms having ability to utilize the phosphorus of ciliatine for growth from feces of sheep, and succeeded to isolate two bacteria which could catabolize the carbon-phosphorus bond. They were identified as E. coli, and named strain Tm-1 and Tm-2. They grew in media of pH 4.5_??_10.5 at 37°C, and the highest rate of decomposition of ciliatine, more than 90% within 30 hr, was obtained in the range of pH 6.5_??_7.5. Though optimal growth on inorganic phosphate and ciliatine occurred at 1.2mm per ml, this organism gave comparable growth rates as well as cell yields when an equimolar amount of either Pi or ciliatine was added to the medium. It seems probable that enzymes splitting C-P bond, chemically resistant to cleavage, may be present in this microorganisms. This microorganisms shows diauxie when grown in the presence of both ciliatine and orthophosphate, the latter being used first. It was found that cells grown on the medium 2 containing ciliatine as sole source of phosphorus were capable of immediate uptake of 32P-ciliatine when suspended in the fresh medium 2 (see Fig. 4) containing 1.2mM ciliatine. However, the cells grown on the medium 1 (see Fig. 8) failed to take up ciliatine under the same conditions described above over periods as long as 4 hr. It was suspected that endogenous phosphate was utilized in preference to the phosphonate.
The mineralization of phosphonates by microorganisms may well contribute to the circulation of phosphorus in nature, because higher animals and plants seem to be unable to break the C-P bond.View full abstractDownload PDF (1213K) -
Yasushi SATO, Ryo NAKAMURA1975 Volume 49 Issue 12 Pages 661-663
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSTo establish a routine method for the detection of antibiotics in egg, both paper disk assay method and TTC test were applied. In case of TTC test, to suppress the antimicrobial action of egg white, diluted egg solutions were previously heated after the digestion with a proteolytic enzyme. In case of paper disk assay method, both whole egg and egg white were used directly, and yolk was used after being slightly diluted with water. Although egg white gave a little high value owing to its antimicrobial action, no adverse effect against paper disk assay method could be found so far as whole egg or diluted yolk was concerned. From the result of this study, paper disk assay method was recommended for the detection of antibiotics in egg.
The results of disk assay carried out on seven kinds of antibiotics dissolved in whole egg, yolk or egg white revealed that penicillin and streptomycin were detected at the level of 0.003-0.005 u/ml and 1_??_2 μg/ml, respectively.View full abstractDownload PDF (244K) -
Hideko NAKAMURA, Kenji WATANABE, Junya MIZUTANI1975 Volume 49 Issue 12 Pages 665-666
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAs taste substances in green-asparagus (Asparagus officinalis L.), taranoki-no-me (leaf buds of Aralia elata Seem.), bracken (Pteridium aquilinum var. latiusculum Und.) and Yamadori-zenmai (Osmundastrum cinnamomeum var. fokiense Tagawa), organic acids were investigated. The organic acids were determined as their n-butyl esters and N- or O-trifluoroacetyl n-butyl esters by gas chromatography on Silicone OV-22. The following results were obtained.
(1) Green-asparagus examined in this investigation contained citric acid as the major acid, amounting to 62% of total organic acids, 19% of malic acid, 16% of pyroglutamic acid and small amounts of succinic and acetic acids.
(2) In taranoki-no-me, quinic acid was dominant, occupying about 78% of total organic acids, and followed by malic and citric acids.
(3) Bracken contained 47% of shikimic acid and 26% of quinic acid.
(4) Yamadori-zenmai contained 26% of citric acid and significant amounts of pyroglutamic, quinic, malic and shikimic acids.View full abstractDownload PDF (154K) -
Tatsuo FUJIKAWA, Takahiko ANNO, Masafuto WADA1975 Volume 49 Issue 12 Pages 667-669
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSSulphated polysaccharide was isolated from thallus of Undaria pinnatifida, Wakame, with CPC and separated into two fractions, Fr-I and Fr-II (major component), by KCl-treatment of the CP-complex.
Characteristic of Fr-I was that arabinose and galactose were contained together with fucose, and the molar ratio fucose: arabinose: xylose: hexose (major component was galactose): glucuronic acid: SO4 was 100:49:15:36:94:67. However, Fr-II was probably galactofucan sulphate and the molar ratio of these components was 100:2:4:64:26:127.
From the standpoints of these components, it is considered that the type of the sulphated polysaccharide of Wakame resembles rather the type of Sargassum (Order Fucales) than the type of Laminaria (Order Laminariales).View full abstractDownload PDF (212K) -
1975 Volume 49 Issue 12 Pages N138
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (38K) -
Kanzo SAKATA1975 Volume 49 Issue 12 Pages R51-R59
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (601K) -
Keiji KAINUMA1975 Volume 49 Issue 12 Pages R61-R67
Published: 1975
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (1764K)
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