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Volume 52, Issue 4
Displaying 1-10 of 10 articles from this issue
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The New Biological Action of Colchicine and VinblastineSchokichi ABE1978 Volume 52 Issue 4 Pages 151-157
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSBlepharismas, a kind of protozoa, were morphologically transformed into spherocells by the action of colchicine, vinlastine and cytochalasin B. But, it was deduced that this morphological transformation was induced by changing the functions of microfilament.
Colchicine and vinblastine had two kinds of actions for blepharismas. The one action was by their own respective biochemical actions which was induced by stereospecific mechanism to the microtubule. But, this action did not have any relations to morphological changes of blepharisma. The other action was such action as ligands to Mn (II) ion which took place by non-stereospecific mechanisms to take off Mn (II) ion from blepharisma. By this action, colchicine and vinblastine made morphological transformations of blepharisma into spherocell as usual other amines.
The discussions were made in this paper on the relationships between the spherocell forming activity of these drugs and their chemical structures.View full abstractDownload PDF (1366K) -
Toyohiko NAKAMURA, Takanori KUROKAWA, Seiichiro NAKATSU, Seinosuke UED ...1978 Volume 52 Issue 4 Pages 159-166
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAn extracellular inulase from Aspergillus niger was purified by Sephadex column chromatography, and was obtained in the crystalline form by ammonium sulfate precipitation. This enzyme hydrolyzed inulin, but not sucrose and raffinose.
The main products from inulin were inulotriose, inulotetraose and inulopentaose, whose amounts were much in his order. The enzyme acted scarcely on inulo-oligosaccharides smaller than pentasaccharide, and the pattern of action seems to be endowise.
The enzyme showed the maximum activity at pH 5.3 and 45°C, and was activated by divalent metal ions such as Mn2+, whereas was markedly inhibited by Hg2+, Ag+, and PCMB. The Km value for inulin was 1.25×10-3M.View full abstractDownload PDF (2169K) -
Metabolic Intermediate and Stereochemical Structure of the Metabolic ProductsOsamu NAKAJIMA, Ryozo IRIYE, Tetsugo HAYASHI1978 Volume 52 Issue 4 Pages 167-174
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSPseudomonas putida strain YK-2 was reported to convert (-)-menthone to (-)-menthol, (+)-isomenthone, 3, 7-dimethy1-3, 6-epoxyoctanoic acid, 3, 7-dimethy1-6-oxooctanoic acid and 3, 7-dimethy1-6-hydroxyoctanoic acid.
One of the metabolic intermediate in the conversion of (-)-menthone was proved to be 3, 7-dimethy1-6-octanolide, which was assumed to be the key compound of the ring fission of (-)-menthone.
So the initial reaction of the metabolism of (-)-menthone was supposed not to be the oxidation of the side chain but the oxygenation between C3-C4 linkage.
Stereochemical Studies of some of above metabolites were also carried out.View full abstractDownload PDF (390K) -
Kinjiro SUKEGAWA, Hiroshi UCHIKAWA, Minoru KATO1978 Volume 52 Issue 4 Pages 175-180
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSTrypsin-like enzyme was separated (0.23mg/g cheese) from three months ripening Gouda cheese by the methods of ammonium sulfate fractionation, gel filtration on Sephadex G-75 and affinity chromatography on a column of 4-aminobenzamidine bound to succinylated aminododecyl cellulose, and this enzyme was found to be almost homogeneous by polyacrylamide disc gel electrophoresis. The trypsin-like enzyme from cheese had an optimum pH at 8.0 and an optimum temperature at 55°C for casein as substrate in 0.1M phosphate buffer, and was stable at pH values from 8.0 to 9.5. These properties were generally similar to bovine trypsin. The two forms of enzyme were found to differ in specific activity toward casein, the bovine trypsin being 12 times more active than the trypsin-like enzyme from cheese, and trypsin-like enzyme moves more slowly than bovine trypsin by polyacrylamide disc gel electrophoresis.View full abstractDownload PDF (1041K) -
Yasuo KONDO1978 Volume 52 Issue 4 Pages 181-186
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSChanges of the total lipid contents and the neutral lipid fractions at different sprouting stages of black matpe up to 7 days were investigated.
The total lipid contents were very small amount and the neutral lipid fractions were lower than polar lipid fractions at each stage of sprouts. Both fractions gradually decreased during germination.
Among the neutral lipid fractions, it had changed remarkably that triglycerides decreased and free sterols and free fatty acids increased after germination. The major component acids in neutral lipid fractions, triglycerides and sterolesters were linolenic and linoleic acids at each period. The former had a tendency to decrease and the latter inclined to increase, whereas the ratio of saturated acids in the total component acids increased during germination.
At each stage of sprouts, it was characterized that the principal molecular species of triglyceride was trilinolenin.View full abstractDownload PDF (1234K) -
Masaaki KUWAHARA1978 Volume 52 Issue 4 Pages 187-189
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIsotopically labeled nicotinic acid and nicotinamide were incorporated into the metabolites of nicotinic acid-dependent pathway (Preiss-Handlerpathway) of the NAD biosynthesis by resting cells of Arthrobacter globiformis. Azaserine and adenosine markedly stimulated the accumulation of NAD in the cells. Radioactive nicotinic acid and nicotinamide were also incorporated into an unknown compound when the cells were incubated in the presence of azaserine.
Cell-free extract of the organism showed the NAD synthetase actÍvÍty, which required ammonium ion and ATP for the amidation of deamido-NAD. Adenosine inhibited the enzyme activity. The organism possessed nicotinamidase, suggesting deamidation is the first step in the biosynthesis of NAD from nicotÍnamÍde. The activity was inhibited by NAD, NADP and NMN.View full abstractDownload PDF (248K) -
Kazuyasu UMEMOTO, Tôru NAGASAWA1978 Volume 52 Issue 4 Pages 191-193
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThis report deals with the examination of the essential oil of Mentha gentilis L. grown in Sanyo area which contains (+)-pulegone (1), (-)-menthone (2) and (+)-neomenthol (3) as the major constituents. The composition of these components in each oil harvested at the blooming time of the plant in the above area and our university was shown as the followings: the former contained 1 (28.9%), 2 (31.0%) and 36 (22.6%), and the latter, 1 (42.6%), 2 (30.8%) and 3 (9.5%). To clarify this discrepancy, the oils collected in the different seasons were examined: the oil from the plant cultivated in our university was shown to contain 1 (44.0%), 2 (28.1%) and 3 (11.2%) in August, and 1 (22.7%), 2 (38.3%) and 3 (28.9%) in September, and 1 (1.8%), 2 (42.4%) and 3 (44.6%) in October, respectively. On the basis of the above fact, it has been found that this gentilis shows different composition of the major components when cultivated at the different places, although the biosynthetic process as pulegone→menthone→neomenthol of the strain is not changed.View full abstractDownload PDF (212K) -
1978 Volume 52 Issue 4 Pages N58
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (56K) -
Hajime NOGUCHI1978 Volume 52 Issue 4 Pages R39-R47
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (731K) -
N. Y1978 Volume 52 Issue 4 Pages N51-N52
Published: 1978
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (165K)
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