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Volume 54, Issue 12
Displaying 1-10 of 10 articles from this issue
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Nobuhiro FUKUDA, Takashi KOJA, Isao CHINEN, Fujiya HONGO, Sadao SHIROM ...1980 Volume 54 Issue 12 Pages 1015-1019
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSInhibitory activity against bovine trypsin and α-chymotrypsin was found to exist in the Leucaena leucocephala seed.
Characterization of the crude inhibitors by disk gel electrophoresis followed by activity staining revealed that 11 trypsin inhibitors were present, four of which also had inhibitory activity against α-chymotrypsin. Purification of the crude inhibitors was accomplished first by adsorption, on DEAE-cellulose and elution from it, and then DEAE-cellulose and DEAE-Sephadex A-25 column chromatography. This partially purified protein showed four bands which closely moved having anti-trypsin and anti-α-chymotrypsin activity in disk gel electrophoresis.View full abstractDownload PDF (1620K) -
Shinsaku HAYASHIDA, Keiko TSUKAMOTO1980 Volume 54 Issue 12 Pages 1021-1025
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSHeavy infections of anti-yeast substance-producing bacteria were formed in six of 45 rice koji samples collected from sake brewery factory. The isolated anti-yeast substance-producing bacteria were identified as Bacillus subtilis, and produced an antibiotic substance toward Saccharomyces sake Kyokai No. 7 in the culture filtrate of koji extract, malt extract and synthetic medium. A typical strain, Bacillus subtilis 16-2 grew well on steamed rice, and showed anti-yeast activity within 24 hr of the koji-making process. The infected koji suppressed fermentation in the mash. The final concentration of alcohol reached 15.6% with the infected koji, whereas 20.0% with normal koji.View full abstractDownload PDF (1002K) -
Sanji MATSUSHIMA, Shigeo ISHIGURO, Shirô SUGAWARA1980 Volume 54 Issue 12 Pages 1027-1035
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe aroma of tobacco essential oil is similar to that of the tobacco leaf and has an effect on the tobacco smoke. The main components of essential oil from flue-cured, Turkish and Burley tobaccos were identified and quantitatively determined by gas chromatography and gas chromatography-mass spectrometry. The aromas of mixtures of the main compounds were evaluated by sensory tests.
The aroma of essential oil from flue-cured tobacco seemed to be formed mainly by a comabination of linolenic acid, linoleic acid, solanone, megastigmatrienone and furfuryl alcohol, while that from Turkish tobacco appeared to be formed by β-methylvaleric acid, linolenic acid and linoleic acid. Compounds contributing to the aroma of essential oil from Burley tobacco could not be determined.View full abstractDownload PDF (553K) -
Isao SAKATA, Koichi KOSHIMIZU1980 Volume 54 Issue 12 Pages 1037-1043
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA study was conducted on seasonal variations of the amount of terpenoids in Japanese peppermint (Mentha arvensis L. var. piperascens Mal.) to determine the appropriate time and the appropriate leaves to harvest. Various parts of the plant were sampled at several developmental stages, and the contents of menthyl glucoside (MG), menthone (MenCO), menthol (MenOH) and other related monoterpenes were determined by gas chromatography before and after acetylation of the methanolic extract of each sample.
The MG content was greatest at the pre-flowering stage. At the end of July MG content was 0.4 μg/mg fresh weight of the whole plant, i.e., 1/25 of MenOH content. At the sprouting stage from late April to early May, the concentration of MG in the intact plant was 0.1 μg/mg fresh weight (1/10 of concentration of MenOH) and that in fresh petals of fully opened flowers in the middle of August, 0.5 μg/mg fresh weight of petals (1/3 of the concentration of MenOH).
The MenCO content was greatest at the first half of the pre-flowering stage (0.37 μg/mg fresh weight of the whole plant) and the MenOH content greatest at the latter half of the pre-lowering stage (11.0 μg/mg fresh weight of the whole plant). Of all leaves on the plant, the first expanded leaf had the highest concentration of MenCO. The concentration of MenOH increased with the aging of the leaf until the onset of flowering, after which it rapidly decreased except for the youngest expanded leaf.
The results of the experiments showed that this herb should be harvested at the pre-flowering stage, i.e., the first half of the rainy season, and should be dried and steam distilled in the presence of acid to obtain good yields of essential oils (crude peppermint oil).View full abstractDownload PDF (480K) -
Kenji ISSHIKI, Shusaku TSUMURA, Tadao WATANABE1980 Volume 54 Issue 12 Pages 1045-1050
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDiphenyl, o-phenylphenol (OPP) and thiabendazole were analyzed simultaneously. Citrus fruits were homogenized, and ammonium acetate was added to adjust the pH to a range from 7 to 10. The three food additives were extracted with ethylacetate. They were analyzed with a FID-gas chromatograph. A glass column was packed with 10% FFAP coated on Chromosorb W (AW. DMCS., 60_??_80 mesh). Its length and inner diameter were 50cm and 3mm, respectively. Column temperature was increased from 110 to 250°C at the rate of 8°C/min, Diphenyl, OPP and thiabendazole were detected at 5.2, 11.4 and 20.8min, respectively. The minimum detectable concentration of each chemical in sample by gas chromatography was 1 ppm.
These chemicals were not detected in 10 kinds of domestic citrus fruits. On the contrary, they were detected in foreign citrus fruits. There were only a few imported citrus fruits that were free of these food additives.View full abstractDownload PDF (401K) -
Tunenori INOUE, Takayuki ASANO, Rikisaku SUEMITSU1980 Volume 54 Issue 12 Pages 1051-1054
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn order to clarify the form in which mercury is transported in a river, we improved the method to analyse low content of mercury and fractionated the river water by membrane filters. We confirmed that the membrane filter does not absorb the mercury unspecifically and therefore it does not affect the measured mercury value of river water. We also examined the bed sediment of the river. Dried bed sediment was fractionated into various particle sizes. The organic carbon content and the mercury concentration of each fractions were estimated. The following results have been obtained. (1) The concentration of mercury of the filtrate decreased with the pore size of membrane filter. (2) The dissolved mercury content, or the mercury content of river water filtered by membrane filter of the smallest pore size, is constant no matter how the total mercury content of river water is. And total mercury content depends upon the mercury content of the floating substance of the river. (3) In the bed sediments mercury is contained much more in finer particles than the larger. Mercury content of each particle size is correlated with the organic carbon content. As a result we concluded that major part of mercury is transported in the forms of both floating substance and bed sediment in the river.View full abstractDownload PDF (253K) -
Chikao NISHINO1980 Volume 54 Issue 12 Pages 1055-1066
Published: 1980
Released on J-STAGE: November 21, 2008
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Hiroshi FUKUI1980 Volume 54 Issue 12 Pages 1067-1073
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (541K) -
[in Japanese]1980 Volume 54 Issue 12 Pages 1093-1095
Published: 1980
Released on J-STAGE: November 21, 2008
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[in Japanese]1980 Volume 54 Issue 12 Pages 1096-1098
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (991K)
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