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Volume 54, Issue 7
Displaying 1-11 of 11 articles from this issue
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Tsuya KOBAYASHI, Kazuo SHIRAI1980 Volume 54 Issue 7 Pages 517-525
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAqueous solutions of DNA bases, deoxynucleosides, deoxynucleotides, and mixtures of DNA base and deoxyribose were γ-irradiated in the presence of air or in nitrogen atmosphere. Each solution was prepared at a concentration of 5×10-4M.
UV absorption spectra were measured directly on the test solutions, while the remaining substances and unaltered liberated bases were determined after paper chromatographic separation. The UV absorption spectra were not always depressed with decreases in remaining substances when treated with higher irradiated dose level. Adenine and cytosine appeared to be more stable in free form than in deoxynucleoside or deoxynucleotide form in nitrogen atmosphere.
Ammonia and inorganic phosphate were also determined. Ammonia formation was low from thymine, thymidine and thymidine monophosphate, which have no amino group in their side chains. It will be noted that nitrogen in ring scarcely contributes to ammonia formation. Inorganic phosphate formation from nucleoside of pyrimidines was greater than from that of purines.
It was observed by CD spectrometry that the conformation change of deoxycytidine by γ-irradiation was greater than that of deoxycytidine 5'-monophosphate.View full abstractDownload PDF (644K) -
Yoshinori ITOH, Yoshiyuki MORISHITA, Kageaki AIBARA1980 Volume 54 Issue 7 Pages 527-534
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAflatoxin B1 (AFB1) is known to be stable at pH 4.0_??_5.0. When exposed to pH 1.0_??_3.0, AFB1 is converted to AFB2a, while it is degraded or inactivated at pH 9.0 or higher. Our earlier work indicated that intestinal microorganisms degrade AFB1. To elucidate the mechanisms of microbial degradation weexamined the effects of pH on AFB1 by the following methods.
Aflatoxin B1 was dissolved in buffer solutions of different pH values (3.0, 5.0, 7.0, 9.0 and 11.0), and the mixture was incubated at 37°C for 0, 24, 48 and 72 hr. The resulting products, before and after extraction with chloroform, were characterized by thin layer chromatography (TLC) and also by fluorescence analysis under ultraviolet (UV) light: Sample I represents the products before extraction; Sample II, the chloroform extract; and Sample III, the aqueous residue.
At pH 5.0 and 7.0, more than 90% of AFB1 was recovered in Samples I and II, regardless of the incubation period. After exposure to pH 9.0 for 24 hr, the recovery of AFB1 in Sample II was about 20%, while it was 0% at pH 11.0. At either pH no appreciable fluorescence was observed in Samples I and III, which upon TLC yielded an ellipsoidal spot. Though the spot was not well demarcated, it had the same Rf value and fluorescence as AFB1. This spot increased in proportion to the decrease in AFB1 recovery in Sample II. The substance of the spot was called AFB1-like substance. Further studies demonstrated that when AFB1 was kept at pH 11.0 for 1 hr, it was converted to the AFB1-like substance, which yielded the same Rf values as AFB1 when developed with eight different solvents but unlike AFB1, did not react with p-anisaldehyde nor with 0.5% Fast Blue Salt B.View full abstractDownload PDF (554K) -
Hiromu KAMEOKA, Seiji HASHIMOTO1980 Volume 54 Issue 7 Pages 535-539
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSSteam volatile oils were obtained by steam distillation of seeds of Brassica juncea, Brassica nirta and Brassica campestris. Each component was isolated from oils by column chromatography and preparative gas chromatography and was identified by IR, NMR, GC-MS and GLC (tR).
A total of 55 components were identified.
The major components were: allyl isothiocyanate, dimethyl trisulfide, 3-butenyl isothiocyanate, 3-phenylpropiononitrile and benzyl isothiocyanate.View full abstractDownload PDF (340K) -
Hitoshi OBATA, Hiromasa MIZUGUCHI, Tai TOKUYAMA1980 Volume 54 Issue 7 Pages 541-544
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSTriose reductone (TR) of enediol type reductone and five kinds of triose reductone-monoanils (enaminol type reductones) were prepared and their activities for stability on lard were compared by modified active oxygen method (AOM) procedure to examine the correlation between the chemical structure and antioxidant property. The order of reductone activity was p-nitroaniline-reductone>p-aminoacetophenone-reductone>p-aminobenzoic acid-reductone>methyl p-aminobenzoate-reductone>p-chloroaniline-reductone>TR>L-ascorbic acid (AH2). The combination of AH2 and 2(3)-butyl-4-hydroxyanisole (BHA) was observed to have slight antioxidative properties in lard, but the combinations of triose reductone-monoanils and BHA were not observed. The kinetic plot for the biomolecular rate period was linear. The AOM hours of triose reductone-monoanils were directly proportional to Hammett's value, except for p-nitroaniline-reductone.View full abstractDownload PDF (258K) -
Toro NAKAHARA, Hidetsugu SASAKI, Yukio KANDA, Hideo TOGANO1980 Volume 54 Issue 7 Pages 545-550
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAnaerobic cultivations of bacterial flora in polluted marine sediment and incubations of diluted sediments have revealed some characteristic features of H2S generation. The total amount of H2S generated at 25°C during a comparatively long period was not less than that at 35°C because of the relationship between supply and demand for hydrogen donor to sulfate-reducing bacteria, though the maximum generation rate was smaller. No generation occurred at 5 and 45°C.
The effect of the addition of hydrogen donor (lactate) depended on the phase of bacterial growth. During early cultivation period when the total number of anaerobes was not large, the addition had little effect on generation because the required amount of hydrogen donor was fully supplied by growth of co-existing anaerobes. The activity of the total anaerobe was largest at salinity of sea water, but H2S generation increased as salinity decreased, revealing the contribution of fresh-water strains. The total amount of organic substances in sediment restricts the total amount of H2S generation, consequently the quantity of organic substances and sulfide in sediment seemed to form a kind of stable equilibrium.View full abstractDownload PDF (465K) -
Kohichi TAKIZAWA, Takamoto SUZUKI, Kenroku FUKUDA1980 Volume 54 Issue 7 Pages 551-553
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe special starch paste termed “Furunori” was traditionally used in Japan for “Kakejiku, ” because the paste possesses an anti-fungal activity and an adequate adhesive power for the mounting.
In this report, the anti-fungal activity was demonstrated by the experimentally produced Furunori under the laboratory condition. It has been shown that the mold mite, Tyrophagus putrescentine growing on the surface pad of fungus in the maturation process of Furunori, participates in the development of the anti-fungal activity.View full abstractDownload PDF (194K) -
Yonosuke IKEDA1980 Volume 54 Issue 7 Pages 555-559
Published: 1980
Released on J-STAGE: November 21, 2008
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Akira KAJI1980 Volume 54 Issue 7 Pages 561-567
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (531K) -
3. Some Supplementary RemarksKei YAMANAKA1980 Volume 54 Issue 7 Pages 589-595
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (561K) -
N. Y.1980 Volume 54 Issue 7 Pages 597-598
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (214K) -
[in Japanese]1980 Volume 54 Issue 7 Pages 598-601
Published: 1980
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (2001K)
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