Trehalose can be used as a material for foods, toiletries, and medicines for its various functions. For example, trehalose protects biological membranes structure against damage by freezing or desiccation. A water-selective pervaporation membrane made of polymide was used to remove the water from a yeast extract and to concentrate the trehalose and ethanol for trehalose crystallization instead of the conventionally method where the trehalose extracted from dried yeast using an ethanol-water solution is concentrated by distillation under reduced pressure and was crystallized by the addition of the pure ethanol. Separation, evaluated by the permeation flux and separation factor, was influenced by the feed rate and the concentration of dissolved materials. Bovine serum albumin and deoxyribonucleic acid (from salmon sperm) were used during the study of the effects of other materials present. When trehalose and these materials were in the mixture, separation was less than when only ethanol and water were present. However, the decrease in the permeation flux was made smaller by an increase in the feed rate, ultrafiltration of the extract, or treatment by activated charcoal. Trehalose could be crystallized from a yeast extract dehydrated with the pervaporation membrane when the temperature of the extract was lowered.
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