巻号一覧
70 巻, 7 号
選択された号の論文の6件中1～6を表示しています
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磯貝 彰1996 年 70 巻 7 号 p. 751-757
発行日: 1996/07/01
公開日: 2008/02/14
ジャーナル フリーPDF形式でダウンロード (781K) -
市原 耿民1996 年 70 巻 7 号 p. 759-766
発行日: 1996/07/01
公開日: 2008/02/14
ジャーナル フリーPDF形式でダウンロード (825K) -
西田 妙子, 西田 宏1996 年 70 巻 7 号 p. 767-772
発行日: 1996/07/01
公開日: 2008/02/14
ジャーナル フリーAn approach is proposed for the measurement of magnesium by chelatometric titration with EDTA in the presence of foreign cations (such as calcium, strontium, barium, or zinc) followed by a mathematical data treatment. Magnesium can be measured using a given equation, Y=1/(b+k)=1/(A+k-ax), where A (ml) is the amount of an EDTA solution added in the titration, a (ml/mg) is the amount of the EDTA solution equivalent to 1 mg of magnesium, x (mg) is the amount of magnesium, b (ml) is the amount of the EDTA solution equivalent to foreign cations, and k=A (ml). An approximate solution of x can be roughly estimated from the shape of the curve in the relation between Y and Y2 values obtained by substituting the arithmetical increasing values with the common difference A/8 a in the range of 0 to A/a into x for the equation. Then a more accurate approximate solution of x is graphically estimated from the rough approximate value of x by using a similar method. By the proposed method, 0.04∼1.5 mg of magnesium in synthetic sample solutions, water samples, soy sauce, and milk, in the presence of foreign cations such as less than 4 mg of calcium, can be measured with recoveries of 99∼103% and a relative standard deviation of 0.13∼8.1%(n=4).抄録全体を表示PDF形式でダウンロード (629K) -
蛭間 稔, 白兼 孝雄, 鈴木 勝1996 年 70 巻 7 号 p. 773-780
発行日: 1996/07/01
公開日: 2008/02/14
ジャーナル フリーMaltose phosphorylase was isolated to homogeneity from a cell-free extract of Enterococcus hirae IFO 3181 by chromatographies with phenyl-Sepharose CL-4 B, QAE-Sephadex A-50, hydroxylapatite, and Sephadex G-200. The enzyme was purified about 40-fold with a yield of 32%, and its specific activity was 29.4 units/mg protein. The molecular weight was estimated to be 220, 000 and 90, 000 by HPLC gel filtration on T.SKgel G 3000 SWXL and SDS-polyacrylamide gel electrophoresis, respectively. The enzyme showed optimum activity around pH 6.5-7.5 and its optimum temperature was about 45°C. The enzyme was stable over the range of pH 5.5-8.0 and retained the activity up to about 55°C. Maltose and nigerose were effective substrates for the enzyme reaction, but it could not act on the other disaccharides tested. The Km for maltose, nigerose, phosphate, and arsenate were 1.90, 1.93, 3.37, and 8.33 mM, respectively. The enzyme activity was almost completely inhibited by AgNO3 and HgCl2, and also strongly inhibited by CuSO4, ZnSO4, and p-chloromercuribenzoic acid.抄録全体を表示PDF形式でダウンロード (769K) -
大野 正博, 河東田 茂義1996 年 70 巻 7 号 p. 781-786
発行日: 1996/07/01
公開日: 2008/02/14
ジャーナル フリーThe sausage (S-) and mycelial (M-) forms of the fission yeast Hasegawaea japonica have been grown in a synthetic medium. The mycelial mutant was obtained by treatment with N-methyl-N-nitro-N-nitrosoguanidine followed by plating on solid medium at 30°C. Although the wild strain formed S-form cells, the mutant strain was stable and had M-form cells in several media. Comparative analysis of their walls showed that the M-form wall had greater quantities of alkali-insoluble glucan and smaller quantities of alkali-soluble glucan than the S-form wall had. The alkali-soluble glucan from the cell wall obtained from the S-form cells contained a larger amount of a-glucan and 1, 6-β-glucan than the fractions from the cell wall obtained from the M-form cells. The alkali-insoluble glucan from the cell wall obtained from the M-form cells contained a larger amount of 1, 3-β-glucan and α-glucan than the fractions from the cell wall obtained from the S-form cells. These results indicated that the glucan contents of alkali-soluble and -insoluble glucan were greatly affected by the shape of H japonica cells抄録全体を表示PDF形式でダウンロード (693K) -
下條 学, 正木 和好, 栗田 恵輔, 福島 和雄1996 年 70 巻 7 号 p. 787-792
発行日: 1996/07/01
公開日: 2008/02/14
ジャーナル フリーBactericidal effects of various chitosan samples from squid pens (SP-chitosan) against the Streptococcus mutans group have been investigated. Antimicrobial action of 0.0005% (5μg/ml) SP-chitosan varied greatly depending on the molecular weight of chitosan as well as the species of oral streptococci. The SP-chitosan samples with molecular weights in the range 70-426 kDa had marked antibacterial activity on all the streptococci tested. S. mutans, S. sobrinus, and S. salivarius turned out to be especially sensitive to the SP-chitosan. The bactericidal effect of the 220 kDa SP-chitosan was observed even in a short incubation time (15 sec.) against S. mutans strain GS 5, and its ED50 was 0.00007% (0.7μg/ml). In addition, its antibacterial activity against the same bacteria was greater with increasing degree of deacetylation of SP-chitosan. These results suggest that the SP-chitosan having a high molecular weight and high degree of deacetylation would be useful as an anti-caries agent.抄録全体を表示PDF形式でダウンロード (696K)
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