Saccostrea echinata (Quoy et Gaimard,1835) named Nise-magaki in Japanese is a tropical oyster species that ranges throughout the tropical zones from Japan through Australia. Owing to the topical nature, its geographical distribution in Japan was recorded only in the Yaeyama Islands below 24°20’ N in 1981. In this report, we first verified an occurrence of Nise-magaki
S. echinata in the temperate zone of Japan. Since
S. echinata is morphologically similar to Magaki
Crassostrea gigas (Thunberg, 1793), a reliable molecular taxonomical method was developed by PCR-RFLP analysis that generated distinctive fingerprints between
S. echinata and
C. gigas. A total of 58 oyster specimens were collected in December 2005 and September 2006 at the intertidal zone in Nichinan, Miyazaki, Japan (31°35’ N). PCR amplification of an apparent 530 bp fragment encoding the mitochondrial 16S ribosomal RNA gene was successfully carried out on all specimens collected, and direct digestion of the PCR products using
SacI restriction enzyme enabled to diagnose 5 specimens of
S. echinata. This result suggests a possible diffusion of tropical Nise-magaki approximately 1,000 Km to the northward along the Kuroshio Current.
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