Experimental animal models are necessary to evaluate food functionality and drug effectiveness on human disease. In vitro experiments using effective enzymes and primary cultured cells or established cell lines are often utilized as first screening method for some functional assay. However final in vivo evaluation for the food function and drug action using animal model is essential. In this review, some spontaneous experimental animal models for human disease, stimulus-induced experimental models in rodents, some mutation model animal or gene transgenic animal model and gene knockout model animals are picked up and presented about the outlines. Especially experimental animal models about life-style related disease for human are described showing some references. On the other hand, we are looking for alternative method for experimental animals from general opinion about protection of all nature animals. It is expected that the new alternative evaluation methods for function of foods and drugs using new gene-technology and cyto-technology without experimental animals are developed in near future.