We conducted a proteomic analysis of the human intervertebral disk to elucidate the molecules that could be effective targets for the prevention and treatment of intervertebral disk degeneration. Through repeated examination, the removal of collagen and proteoglycans, which accounted for most of the proteins, was found to be necessary in order to observe any intracellular changes. Therefore we examined the protein fraction in which we eliminated polymers > 100 kDa with a filter using LC-MS/MS. We gathered intervertebral disks from cases of degenerative spondylolisthesis (6 samples), lumbar disc herniation (7 samples), and autopsy specimens (5 samples), and prepared the respective protein extracts. We performed LC-MS/MS by ABI QSTAR on these three groups and assessed the disease-specific proteomic profiles. It was suggested that activation of complement and natural immunity were related to degenerative spondylolisthesis, and that MIF, Gelsolin, MINT were related to lumbar disc herniation. A proteomic analysis of the human intervertebral disk organization was possible using LC-MS/MS. The protein expression profiles that are characteristic of diseases were elucidated.
Objective: To report the annual operation statistics of the department of Surgery at the Nihon University School of Medicine for 2013, and examine future problems. Material and Method: We investigated the kind and number of operations in the divisions of Digestive Surgery, Cardiovascular Respiratory and General Surgery, Pediatric, Breast and Endocrine Surgery at Itabashi Hospital of Nihon University School of Medicine, and Surugadai Nihon University Hospital from January to December 2013. Results: The total number of operations decreased from 2,990 in 2012 to 2,933 in 2013. The number of operations in Pediatric surgery increased, but other surgeries decreased instead due to the increasing number of conservative treatments. The operations for cancer were similar to 2012 in all divisions of the department of Surgery. Conclusion: We propose that it is important to cooperate with the doctors in internal medicine to increase the number of operations.
Background: Intra-isthmus reentry (IIR) denotes a reentry circuit within the cavotricuspid isthmus (CTI). The purpose of this study was to define the electrogram and surface ECG characteristics of IIR. Methods and Results: Three patients underwent electrophysiologic examination and were found to have IIR; the IIR in 1 patient was found during a redo procedure. Detailed electrogram mapping of the CTI and electroanatomic mapping (EAM) were performed in all 3 patients. Entrainment mapping in all patients during tachycardia proved reentry and showed that the anteroinferior CTI was outside the circuit and that the septal CTI was within the circuit. During tachycardia, fractionated or double potentials were recorded at either the septal CTI and/or the region of the coronary sinus ostium (CSos). The surface ECG showed a typical counterclockwise (CCW) pattern in 2 patients and an atypical pattern in 1 patient, and EAM showed a CCW pattern in 2 patients and a focal pattern in 1 patient. The one patient with atypical ECG pattern in spite of the CCW activation pattern showed a low voltage area at the inferolateral tricuspid annulus. Conclusions: IIR presents as a tachycardia circuit confined to the septal CTI/CSos region, and surface ECG characteristics show either typical CTI-dependent AFL or atypical AFL patterns.
A 69-year-old woman was referred to our hospital following the observation of a splenic tumor during abdominal ultrasonography at a nearby clinic, after the indication of diabetes in a medical checkup. After further inspection, malignant disease could not be denied and laparoscopic splenectomy was performed. The operative time was two hours and six minutes, with a small amount of bleeding and an uneventful postoperative course. The pathological outcome was lymphangioma of the spleen. Splenectomy is performed for splenic tumors that are difficult to differentiate. However in such circumstances, laparoscopic splenectomy is considered to be a useful and less invasive method.