Four aminoazobenzene derivatives were injected subcutaneously to pregnant mice of the Japanese dd strain and teratogenic and lethal effects on their offspring were demonstrated. The results may be summarized as follows:
1. Monomethylaminoazobenzene was administered once or twice on the 9th to 11th day of gestation in the dosage of 0.075 to 0.6 mg. per gm. of body weight and found to induce the resorption of embryos in most cases and skeletal abnormalities such as cleft palate and deviation of the toes in a few cases.
2.
m-Fluorodimethylaminoazobenzene was administered once on the 9th to 11th day of gestation in a dosage of 0.15 to 1.2 mg. per gm. of body weight and found to have similar effects to monomethylaminoazobenzene.3.0.5-0.7 mg. of
p-chlorodimethylaminoazobenzene per gm. of body weight was injected on various days in the second trimester and it was shown that this compound has a lethal effect on the embryos in many cases when applied between the 8th and the 11th day, and occasionally induces the malformations in the extremities as well as some cases of cleft palates and abnormalities of the trunk when applied between the 8th and the 14th day. The teratogenic effect was most manifest in cases treated on the 9th day.
4.0.2 mg. of dimethylaminoazobenzene per gm. of body weight was injected on various days in the second trimester of gestation and it was shown that this dye induces embryonal resorption in many casez-, and skeletal abnormalities such as deviations of the toes, polydactyly when applied between the 7th and 14th day. The terato- genic effect was most active in cases treated on the 10th day.
5. The principal teratogenic effect of those four compounds was on the skeletal system, especially on the extremities, although some cases of cleft palate, hematoma and/or abnormality of the trunk were induced by certain compounds.
6. By macroscopic and routine histological examinations, no anomaly was found in the placentas of the treated animals.
7. Monomethylaminoazobenzene was not demonstrated, by the paper chromatographic method, in the uteri, placentas and fetuses from the mothers treated with this dye.
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