In order to investigate the movement of tissue fluid in the living state, dyes in solution or semicolloidal form which pass the blood vessels with ease were introduced into the mesenteric arteries of rabbits, and the movement and spread of these in the tissues after extravasation from the blood vessels were observed.
(1) Irrespective of the difference in mode and site of extravasation according to type of dye, all the extravasated dyes were found to spread in the connective tissue evenly to the surroundings, so that the figures of spread at the beginning and end were similar in shape. Spread occured not by special pathways along fibers and blood vessels. This indicates that the movement of the extravasated dye fluids in the tissue occurs by diffusion.
(2) The spread of these dye fluids was influenced by the regional blood flow. When the local blood circulation stops, the spread of dye practically ceases. When the spread reaches a definite extent, the dye disappears, and the speed of this disappearance is influenced by the blood flow.
(3) The extent of spread in the tissue of the dye extravasated from the blood vessels is small. From this it is believed that spread of tissue fluid is correspondingly limited and though expressions such as circulation or flow of tissue fluid were used, these have to be considered to be localized phenomena and not applicable to the circulation over the entire body.
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