The electron microscopic localization of phosphatases was observed in adult dogs, and that of cytochrome oxidase in an old dog. In the parenchymal cells, intense activities of alkaline phosphatae and adenosine triphosphatase were demonstrated both on the plasma membranes facing the perivascular space and on their transitional portions to the lateral plasma membranes. However, they were less intense or negative on the remaining plasma membranes, suggesting that the plasma membranes of the parenchymal cells have a functional polarity.
In the parenchymal cells, activities of thiamine pyrophosphatase and inosine diphosphatase were restricted to one or two layers of cisternae or saccules at the inner face of the Golgi apparatus, and in some chief cells the latter enzyme activities were also detected on the cisternae of the granular endoplasmic reticulum. On the other hand, acid phosphatase activities were observed on almost the entire Golgi lamellae and on most of their associated vacuoles and vesicles, besides lysosomes and some lipofuscin granules. Acid phosphatase-positive GERL-like structures were found surrounded by Golgi lamellae.
In the blood vessels including the capillaries, activities of all the phosphatases tested except the acid type were positively observed, particularly on the caveolae or pinocytotic vesicles along the capillary luminal surface and the apposing plasma membranes between endothelial and smooth muscle cells.
Cytochrome oxidase activities were localized in the mitochondrial membranes including the cristae, but were variable in intensity even in each mitochondrion within a single cell as well as among the parenchymal cells. Some of the bizarre mitochondria with longitudinal cristae which were characteristic of the old dog parathyroid, exhibited strong activities on their cristae.
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